TY  - JOUR
AU  - Korićanać, Goran
AU  - Hricovíniová, Jana
AU  - Romić, Snježana
AU  - Dobrocsyova, Viktoria
AU  - Stojiljković, Mojca
AU  - Kršková, Katarína
AU  - Ivković, Tamara
AU  - Zorad, Štefan
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13298
AB  - The aim of this study was to improve insulin sensitivity in fructose-treated animals by ingestion of flavonoid quercetin. Several signs of insulin resistance have been developed in rats by drinking 10% fructose solution for 9 weeks. The effect of 6-week-gavage-administrated quercetin (20 mg/kg/day in 1% methyl cellulose solution) was monitored. Rats of the control groups received methyl cellulose vehicle as well. The most striking result of the quercetin treatment was the normalization of the fructose solution drinking to the level of drinking water intake. In addition, quercetin supplementation considerably decreased the plasma glucose and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index in rats consuming fructose. Surprisingly, fructose ingestion did not elevate plasma uric acid, thiobarbituric acid reactive substances, nitrotyrosine, or advanced glycation end products fluorescence. Instead, a reduction of the above parameters was observed. In summary, these results indicate that quercetin supplementation reduces fructose drinking and decreases plasma glucose and the HOMA-IR index. Furthermore, methyl cellulose, in combination with fructose, causes uric acid – lowering, antioxidant and anti-glycation effects. Thus, methyl cellulose possibly shifts fructose metabolism in favor of the utilization of antioxidant features of fructose. Our results call for using methyl cellulose in sweetened beverages and other sweetened food.
T2  - General physiology and biophysics
T1  - Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Unexpected uric acid and TBARS lowering effect of methyl cellulose vehicle and fructose combination
VL  - 43
IS  - 3
SP  - 221
EP  - 230
DO  - 10.1016/j.freeradbiomed.2023.03.190
ER  - 

@article{
author = "Korićanać, Goran and Hricovíniová, Jana and Romić, Snježana and Dobrocsyova, Viktoria and Stojiljković, Mojca and Kršková, Katarína and Ivković, Tamara and Zorad, Štefan",
year = "2024",
abstract = "The aim of this study was to improve insulin sensitivity in fructose-treated animals by ingestion of flavonoid quercetin. Several signs of insulin resistance have been developed in rats by drinking 10% fructose solution for 9 weeks. The effect of 6-week-gavage-administrated quercetin (20 mg/kg/day in 1% methyl cellulose solution) was monitored. Rats of the control groups received methyl cellulose vehicle as well. The most striking result of the quercetin treatment was the normalization of the fructose solution drinking to the level of drinking water intake. In addition, quercetin supplementation considerably decreased the plasma glucose and Homeostatic Model Assessment for Insulin Resistance (HOMA-IR) index in rats consuming fructose. Surprisingly, fructose ingestion did not elevate plasma uric acid, thiobarbituric acid reactive substances, nitrotyrosine, or advanced glycation end products fluorescence. Instead, a reduction of the above parameters was observed. In summary, these results indicate that quercetin supplementation reduces fructose drinking and decreases plasma glucose and the HOMA-IR index. Furthermore, methyl cellulose, in combination with fructose, causes uric acid – lowering, antioxidant and anti-glycation effects. Thus, methyl cellulose possibly shifts fructose metabolism in favor of the utilization of antioxidant features of fructose. Our results call for using methyl cellulose in sweetened beverages and other sweetened food.",
journal = "General physiology and biophysics",
title = "Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Unexpected uric acid and TBARS lowering effect of methyl cellulose vehicle and fructose combination",
volume = "43",
number = "3",
pages = "221-230",
doi = "10.1016/j.freeradbiomed.2023.03.190"
}

Korićanać, G., Hricovíniová, J., Romić, S., Dobrocsyova, V., Stojiljković, M., Kršková, K., Ivković, T.,& Zorad, Š.. (2024). Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Unexpected uric acid and TBARS lowering effect of methyl cellulose vehicle and fructose combination. in General physiology and biophysics, 43(3), 221-230.
https://doi.org/10.1016/j.freeradbiomed.2023.03.190

Korićanać G, Hricovíniová J, Romić S, Dobrocsyova V, Stojiljković M, Kršková K, Ivković T, Zorad Š. Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Unexpected uric acid and TBARS lowering effect of methyl cellulose vehicle and fructose combination. in General physiology and biophysics. 2024;43(3):221-230.
doi:10.1016/j.freeradbiomed.2023.03.190 .

Korićanać, Goran, Hricovíniová, Jana, Romić, Snježana, Dobrocsyova, Viktoria, Stojiljković, Mojca, Kršková, Katarína, Ivković, Tamara, Zorad, Štefan, "Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Unexpected uric acid and TBARS lowering effect of methyl cellulose vehicle and fructose combination" in General physiology and biophysics, 43, no. 3 (2024):221-230,
https://doi.org/10.1016/j.freeradbiomed.2023.03.190 . .

TY  - CONF
AU  - Hricovíniová, Jana
AU  - Korićanac, Goran
AU  - Romić, Snježana
AU  - Kršková, Katarína
AU  - Bozorgnia, Mahdi
AU  - Zorad, Štefan
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13299
AB  - The excessive fructose consumption is linked with metabolic changes and serious health problems. Overconsumption of fructose-sweetened food products mainly sugar-sweetened beverages is associated with body-weight gain, obesity, inflammation and cardiometabolic disturbances. The majority of fructose is metabolized in liver and an excess of fructose is converted to fat. The triglycerides are taken up by the adipose tissue for storage– consequently the fructose fed rats display enhancement of the adipose tissue mass and elevated blood uric acid. High fructose intake increases oxidation stress. Quercetin is one of the most commonly occurring bioactive flavonoids in human foods. It is present in tea, apples, broccoli, onion berries and red grapes. The quercetin is known for its beneficial health effects including mainly anti-inflammatory and antioxidant properties. In the presented study we adopted rats with fully developed fructose induced insulin resistance (9 weeks of 10% fructose treatment) which were then continuously treated with combination of fructose and 20 mg/kg/day quercetin for additional 6 weeks. Quercetin was delivered by gavage as solubilized in 1% methyl cellulose. Our results showed that quercetin treatment lead to the normalization of the fructose solution drinking to the level of drinking water intake as well as the decrease of plasma glucose in rats consuming fructose. Moreover, a significant decrease of plasma uric acid, oxidative stress biomarkers (TBARS, nitrotyrosine), AGEs fluorescence and hemoglobin leakage was induced by combination of fructose with methyl cellulose. Thus, methyl cellulose with fructose had positive impacts like antioxidant, anti-glycation and erythrocyteprotective effects. We might speculate that methyl cellulose possibly shifts the fructose metabolism in favour of utilization of antioxidant features of fructose. Moreover, in vitro experiments showed that fructose and methyl cellulose had a moderate antioxidant activity with additive effect showed in the ferric reducing antioxidant power assay.
PB  - Elsevier BV
C3  - Free Radical Biology and Medicine
T1  - Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Antioxidant effects of fructose - methyl cellulose combination
VL  - 201
IS  - Supplement 1
SP  - 47
EP  - 47
DO  - 10.1016/j.freeradbiomed.2023.03.190
ER  - 

@conference{
author = "Hricovíniová, Jana and Korićanac, Goran and Romić, Snježana and Kršková, Katarína and Bozorgnia, Mahdi and Zorad, Štefan",
year = "2023",
abstract = "The excessive fructose consumption is linked with metabolic changes and serious health problems. Overconsumption of fructose-sweetened food products mainly sugar-sweetened beverages is associated with body-weight gain, obesity, inflammation and cardiometabolic disturbances. The majority of fructose is metabolized in liver and an excess of fructose is converted to fat. The triglycerides are taken up by the adipose tissue for storage– consequently the fructose fed rats display enhancement of the adipose tissue mass and elevated blood uric acid. High fructose intake increases oxidation stress. Quercetin is one of the most commonly occurring bioactive flavonoids in human foods. It is present in tea, apples, broccoli, onion berries and red grapes. The quercetin is known for its beneficial health effects including mainly anti-inflammatory and antioxidant properties. In the presented study we adopted rats with fully developed fructose induced insulin resistance (9 weeks of 10% fructose treatment) which were then continuously treated with combination of fructose and 20 mg/kg/day quercetin for additional 6 weeks. Quercetin was delivered by gavage as solubilized in 1% methyl cellulose. Our results showed that quercetin treatment lead to the normalization of the fructose solution drinking to the level of drinking water intake as well as the decrease of plasma glucose in rats consuming fructose. Moreover, a significant decrease of plasma uric acid, oxidative stress biomarkers (TBARS, nitrotyrosine), AGEs fluorescence and hemoglobin leakage was induced by combination of fructose with methyl cellulose. Thus, methyl cellulose with fructose had positive impacts like antioxidant, anti-glycation and erythrocyteprotective effects. We might speculate that methyl cellulose possibly shifts the fructose metabolism in favour of utilization of antioxidant features of fructose. Moreover, in vitro experiments showed that fructose and methyl cellulose had a moderate antioxidant activity with additive effect showed in the ferric reducing antioxidant power assay.",
publisher = "Elsevier BV",
journal = "Free Radical Biology and Medicine",
title = "Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Antioxidant effects of fructose - methyl cellulose combination",
volume = "201",
number = "Supplement 1",
pages = "47-47",
doi = "10.1016/j.freeradbiomed.2023.03.190"
}

Hricovíniová, J., Korićanac, G., Romić, S., Kršková, K., Bozorgnia, M.,& Zorad, Š.. (2023). Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Antioxidant effects of fructose - methyl cellulose combination. in Free Radical Biology and Medicine
Elsevier BV., 201(Supplement 1), 47-47.
https://doi.org/10.1016/j.freeradbiomed.2023.03.190

Hricovíniová J, Korićanac G, Romić S, Kršková K, Bozorgnia M, Zorad Š. Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Antioxidant effects of fructose - methyl cellulose combination. in Free Radical Biology and Medicine. 2023;201(Supplement 1):47-47.
doi:10.1016/j.freeradbiomed.2023.03.190 .

Hricovíniová, Jana, Korićanac, Goran, Romić, Snježana, Kršková, Katarína, Bozorgnia, Mahdi, Zorad, Štefan, "Quercetin decreases fructose drinking in model of fructose-induced insulin resistance. Antioxidant effects of fructose - methyl cellulose combination" in Free Radical Biology and Medicine, 201, no. Supplement 1 (2023):47-47,
https://doi.org/10.1016/j.freeradbiomed.2023.03.190 . .

TY  - JOUR
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Stanišić, Jelena
AU  - Romić, Snježana Đ.
AU  - Ćulafić, Tijana
AU  - Ivković, Tamara
AU  - Stojiljković, Mojca D.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10747
AB  - Background and Aim: Excessive fructose consumption along with a sedentary lifestyle provokes metabolic disorders and cardiovascular diseases. Fructose overload causes cardiac insulin resistance and increases reliance on fatty acid (FA) uptake and catabolism. The cardiometabolic benefits of exercise training have long been appreciated. The goal of the presented study is to shed a new light to the preventive role of exercise training on cardiac lipid metabolism in fructose-fed rats. Methods: Male Wistar rats were divided into control (C), sedentary fructose (F), and exercised fructose (EF) groups. Fructose was given as a 10% fructose solution in drinking water for 9 weeks. Low-intensity exercise training was applied for 9 weeks. The protein expression and subcellular localization of Lipin1, peroxisome proliferator-activated receptor α (PPARα), and peroxisome proliferator-activated receptor-γcoactivator 1 α (PGC1) were analyzed in the heart using Western blot. Cardiac forkhead box transcription factor 1 (FOXO1) and sirtuin 1 (SIRT1) protein levels were also evaluated. Gene expression of long-chain acyl-CoA dehydrogenase was analyzed by quantitative polymerase chain reaction. Results: Exercise training has augmented the expression of main regulators of FA oxidation in the heart and achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1 compared with the fructose group (P = 0.0422, P = 0.000045, P = 0.00958, respectively). In addition, Lipin1, FOXO1, and SIRT1 were increased in nuclear extract after exercise compared with the control group (P = 0.000043, P = 0.0417, P = 0.0329, respectively). In cardiac lysate, low-intensity exercise caused significantly increased protein level of PPARα, PGC1, FOXO1, and SIRT1 compared with control (P = 0.0377, P = 0.0275, P = 0.0096, P = 0.0282, respectively) and PGC1 level compared with the fructose group (P = 0.0417). Conclusion: The obtained results imply that the heart with a metabolic burden additionally relies on FA as an energy substrate after low-intensity running. © Copyright 2023, Mary Ann Liebert, Inc., publishers 2023.
T2  - Metabolic Syndrome and Related Disorders
T1  - Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats
VL  - 21
IS  - 2
SP  - 122
EP  - 131
DO  - 10.1089/met.2022.0078
ER  - 

@article{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Stanišić, Jelena and Romić, Snježana Đ. and Ćulafić, Tijana and Ivković, Tamara and Stojiljković, Mojca D.",
year = "2023",
abstract = "Background and Aim: Excessive fructose consumption along with a sedentary lifestyle provokes metabolic disorders and cardiovascular diseases. Fructose overload causes cardiac insulin resistance and increases reliance on fatty acid (FA) uptake and catabolism. The cardiometabolic benefits of exercise training have long been appreciated. The goal of the presented study is to shed a new light to the preventive role of exercise training on cardiac lipid metabolism in fructose-fed rats. Methods: Male Wistar rats were divided into control (C), sedentary fructose (F), and exercised fructose (EF) groups. Fructose was given as a 10% fructose solution in drinking water for 9 weeks. Low-intensity exercise training was applied for 9 weeks. The protein expression and subcellular localization of Lipin1, peroxisome proliferator-activated receptor α (PPARα), and peroxisome proliferator-activated receptor-γcoactivator 1 α (PGC1) were analyzed in the heart using Western blot. Cardiac forkhead box transcription factor 1 (FOXO1) and sirtuin 1 (SIRT1) protein levels were also evaluated. Gene expression of long-chain acyl-CoA dehydrogenase was analyzed by quantitative polymerase chain reaction. Results: Exercise training has augmented the expression of main regulators of FA oxidation in the heart and achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1 compared with the fructose group (P = 0.0422, P = 0.000045, P = 0.00958, respectively). In addition, Lipin1, FOXO1, and SIRT1 were increased in nuclear extract after exercise compared with the control group (P = 0.000043, P = 0.0417, P = 0.0329, respectively). In cardiac lysate, low-intensity exercise caused significantly increased protein level of PPARα, PGC1, FOXO1, and SIRT1 compared with control (P = 0.0377, P = 0.0275, P = 0.0096, P = 0.0282, respectively) and PGC1 level compared with the fructose group (P = 0.0417). Conclusion: The obtained results imply that the heart with a metabolic burden additionally relies on FA as an energy substrate after low-intensity running. © Copyright 2023, Mary Ann Liebert, Inc., publishers 2023.",
journal = "Metabolic Syndrome and Related Disorders",
title = "Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats",
volume = "21",
number = "2",
pages = "122-131",
doi = "10.1089/met.2022.0078"
}

Kostić, M., Korićanac, G., Tepavčević, S., Stanišić, J., Romić, S. Đ., Ćulafić, T., Ivković, T.,& Stojiljković, M. D.. (2023). Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats. in Metabolic Syndrome and Related Disorders, 21(2), 122-131.
https://doi.org/10.1089/met.2022.0078

Kostić M, Korićanac G, Tepavčević S, Stanišić J, Romić SĐ, Ćulafić T, Ivković T, Stojiljković MD. Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats. in Metabolic Syndrome and Related Disorders. 2023;21(2):122-131.
doi:10.1089/met.2022.0078 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Stanišić, Jelena, Romić, Snježana Đ., Ćulafić, Tijana, Ivković, Tamara, Stojiljković, Mojca D., "Low-Intensity Exercise Affects Cardiac Fatty Acid Oxidation by Increasing the Nuclear Content of PPARα, FOXO1, and Lipin1 in Fructose-Fed Rats" in Metabolic Syndrome and Related Disorders, 21, no. 2 (2023):122-131,
https://doi.org/10.1089/met.2022.0078 . .

TY  - JOUR
AU  - Ivković, Tamara
AU  - Tepavčević, Snežana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Ćulafić, Tijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10924
AB  - Cholecalciferol improves insulin signaling and glucose metabolism in the heart and reduces
circulating non-esterified fatty acids. Cholecalciferol effects on the cardiac fatty acid (FA) metabolism
and the consequences on calcium handling were examined. Blood lipid profile was determined.
Western blot and qRT-PCR were used to examine protein and mRNA expression. Cholecalciferoltreated rats had increased acetyl CoA carboxylase 2 protein expression and decreased expression of
malonyl CoA decarboxylase. In addition, the expression of uncoupling protein 3 was elevated. Also,
the level of peroxisome proliferator-activated receptor-gamma coactivator in the nucleus of heart
cells was increased along with the level of sarcoplasmic/endoplasmic reticulum Ca2+ATPase in the
microsomal fraction. In parallel, the L-type calcium channel and ryanodine receptor expression was
reduced. In the heart of healthy rats, cholecalciferol affects proteins regulating malonyl CoA availability and intracellular Ca2+ handling proteins.
T2  - General physiology and biophysics
T1  - Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level
VL  - 42
IS  - 03
SP  - 241
EP  - 250
DO  - 10.4149/gpb_2023005
ER  - 

@article{
author = "Ivković, Tamara and Tepavčević, Snežana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Stanišić, Jelena and Korićanac, Goran and Ćulafić, Tijana",
year = "2023",
abstract = "Cholecalciferol improves insulin signaling and glucose metabolism in the heart and reduces
circulating non-esterified fatty acids. Cholecalciferol effects on the cardiac fatty acid (FA) metabolism
and the consequences on calcium handling were examined. Blood lipid profile was determined.
Western blot and qRT-PCR were used to examine protein and mRNA expression. Cholecalciferoltreated rats had increased acetyl CoA carboxylase 2 protein expression and decreased expression of
malonyl CoA decarboxylase. In addition, the expression of uncoupling protein 3 was elevated. Also,
the level of peroxisome proliferator-activated receptor-gamma coactivator in the nucleus of heart
cells was increased along with the level of sarcoplasmic/endoplasmic reticulum Ca2+ATPase in the
microsomal fraction. In parallel, the L-type calcium channel and ryanodine receptor expression was
reduced. In the heart of healthy rats, cholecalciferol affects proteins regulating malonyl CoA availability and intracellular Ca2+ handling proteins.",
journal = "General physiology and biophysics",
title = "Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level",
volume = "42",
number = "03",
pages = "241-250",
doi = "10.4149/gpb_2023005"
}

Ivković, T., Tepavčević, S., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Stanišić, J., Korićanac, G.,& Ćulafić, T.. (2023). Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level. in General physiology and biophysics, 42(03), 241-250.
https://doi.org/10.4149/gpb_2023005

Ivković T, Tepavčević S, Romić SĐ, Stojiljković MD, Kostić M, Stanišić J, Korićanac G, Ćulafić T. Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level. in General physiology and biophysics. 2023;42(03):241-250.
doi:10.4149/gpb_2023005 .

Ivković, Tamara, Tepavčević, Snežana, Romić, Snježana Đ., Stojiljković, Mojca D., Kostić, Milan, Stanišić, Jelena, Korićanac, Goran, Ćulafić, Tijana, "Cholecalciferol affects cardiac proteins regulating malonyl-CoA availability and intracellular calcium level" in General physiology and biophysics, 42, no. 03 (2023):241-250,
https://doi.org/10.4149/gpb_2023005 . .

TY  - JOUR
AU  - Tepavčević, Snežana
AU  - Romić, Snježana
AU  - Zec, Manja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca
AU  - Ivković, Tamara
AU  - Pantelić, Marija
AU  - Kostić, Milan
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11987
AB  - The transport of cations in the cardiomyocytes, crucial for the functioning of the heart, can be affected by walnut diet due to the high content of polyunsaturated fatty acids. Healthy and metabolically compromised rats (drinking 10% fructose solution) were subjected to a diet supplemented with 2.4 g of walnuts for 6 weeks to investigate the effect on proteins involved in cation transport in the heart cells. Fructose increased the level of the a1 subunit of Na+/K+-ATPase and the phosphorylation of extracellular signal-regulated kinase 1/2 in the heart of control and walnut-eating rats, while elevated L-type calcium channel a (LTCCa), sodium–calcium exchanger 1 (NCX1), and Maxi Ka level were observed only in rats that did not consume walnuts. However, walnuts significantly increased the cardiac content of LTCC, NCX1, and Maxi Ka, as well as Kir6.1 and SUR2B subunits of KATP channel, but only in fructose-naive rats. In animals that drank fructose, a significant increasing effect of walnuts was observed only in Akt kinase phosphorylation, which may be a part of the antiarrhythmic mechanism of decreasing cation currents in cardiomyocytes. The walnut diet-induced increase in LTCC and NCX1 expression in healthy rats may indicate intense cardiac calcium turnover, whereas the effect on Kir6.1 and SUR2B subunits suggests stimulation of KATP channel transport in the cardiac vasculature. The effects of walnuts on the cation-handling proteins in the heart, mostly limited to healthy animals, suggest the possible use of a walnut-supplemented diet in the prevention rather than the treatment of cardiological channelopathies.
T2  - Journal of Medicinal Food
T1  - Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats
VL  - 26
IS  - 11
SP  - 849
EP  - 857
DO  - 10.1089/jmf.2022.0157
ER  - 

@article{
author = "Tepavčević, Snežana and Romić, Snježana and Zec, Manja and Ćulafić, Tijana and Stojiljković, Mojca and Ivković, Tamara and Pantelić, Marija and Kostić, Milan and Stanišić, Jelena and Korićanac, Goran",
year = "2023",
abstract = "The transport of cations in the cardiomyocytes, crucial for the functioning of the heart, can be affected by walnut diet due to the high content of polyunsaturated fatty acids. Healthy and metabolically compromised rats (drinking 10% fructose solution) were subjected to a diet supplemented with 2.4 g of walnuts for 6 weeks to investigate the effect on proteins involved in cation transport in the heart cells. Fructose increased the level of the a1 subunit of Na+/K+-ATPase and the phosphorylation of extracellular signal-regulated kinase 1/2 in the heart of control and walnut-eating rats, while elevated L-type calcium channel a (LTCCa), sodium–calcium exchanger 1 (NCX1), and Maxi Ka level were observed only in rats that did not consume walnuts. However, walnuts significantly increased the cardiac content of LTCC, NCX1, and Maxi Ka, as well as Kir6.1 and SUR2B subunits of KATP channel, but only in fructose-naive rats. In animals that drank fructose, a significant increasing effect of walnuts was observed only in Akt kinase phosphorylation, which may be a part of the antiarrhythmic mechanism of decreasing cation currents in cardiomyocytes. The walnut diet-induced increase in LTCC and NCX1 expression in healthy rats may indicate intense cardiac calcium turnover, whereas the effect on Kir6.1 and SUR2B subunits suggests stimulation of KATP channel transport in the cardiac vasculature. The effects of walnuts on the cation-handling proteins in the heart, mostly limited to healthy animals, suggest the possible use of a walnut-supplemented diet in the prevention rather than the treatment of cardiological channelopathies.",
journal = "Journal of Medicinal Food",
title = "Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats",
volume = "26",
number = "11",
pages = "849-857",
doi = "10.1089/jmf.2022.0157"
}

Tepavčević, S., Romić, S., Zec, M., Ćulafić, T., Stojiljković, M., Ivković, T., Pantelić, M., Kostić, M., Stanišić, J.,& Korićanac, G.. (2023). Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats. in Journal of Medicinal Food, 26(11), 849-857.
https://doi.org/10.1089/jmf.2022.0157

Tepavčević S, Romić S, Zec M, Ćulafić T, Stojiljković M, Ivković T, Pantelić M, Kostić M, Stanišić J, Korićanac G. Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats. in Journal of Medicinal Food. 2023;26(11):849-857.
doi:10.1089/jmf.2022.0157 .

Tepavčević, Snežana, Romić, Snježana, Zec, Manja, Ćulafić, Tijana, Stojiljković, Mojca, Ivković, Tamara, Pantelić, Marija, Kostić, Milan, Stanišić, Jelena, Korićanac, Goran, "Effects of Walnut-Rich Diet on Cation-Handling Proteins in the Heart of Healthy and Metabolically Compromised Male Rats" in Journal of Medicinal Food, 26, no. 11 (2023):849-857,
https://doi.org/10.1089/jmf.2022.0157 . .

TY  - JOUR
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Stanišić, Jelena
AU  - Romić, Snježana
AU  - Ćulafić, Tijana
AU  - Ivković, Tamara
AU  - Stojiljković, Mojca
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13109
AB  - Context: Excessive fructose consumption causes ectopic lipid storage leading to metabolic disorders and cardiovascular diseases associated with defective substrate utilisation in the heart. Objective: Examining the preventive impact of low-intensity exercise on alterations related to fructose-rich diet (FRD) on cardiac fatty acid (FA) transport and metabolism. Materials and methods: Male Wistar rats were divided into control and two groups that received 10% fructose for 9 weeks, one of which was additionally exposed to exercise. Results: FRD elevated plasma and cardiac TAG, FATP1 in plasma membrane, Lipin 1 in microsomes and HSL mRNA, while mitochondrial CPT1 was decreased. Exercise decreased plasma free FA level, raised CD36 in plasma membrane and FATP1 in lysate, mitochondrial CPT1 and decreased microsomal Lipin 1 in fructose-fed rats. Conclusions: FRD changed plasma lipids and augmented partitioning of FA to TAG storage in the heart, whereas exercise in FRD rats switched metabolism of FA towards β-oxidation.
T2  - Archives of Physiology and Biochemistry
T1  - Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats
VL  - 129
IS  - 4
SP  - 922
EP  - 932
DO  - 10.1080/13813455.2021.1886118
ER  - 

@article{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Stanišić, Jelena and Romić, Snježana and Ćulafić, Tijana and Ivković, Tamara and Stojiljković, Mojca",
year = "2023",
abstract = "Context: Excessive fructose consumption causes ectopic lipid storage leading to metabolic disorders and cardiovascular diseases associated with defective substrate utilisation in the heart. Objective: Examining the preventive impact of low-intensity exercise on alterations related to fructose-rich diet (FRD) on cardiac fatty acid (FA) transport and metabolism. Materials and methods: Male Wistar rats were divided into control and two groups that received 10% fructose for 9 weeks, one of which was additionally exposed to exercise. Results: FRD elevated plasma and cardiac TAG, FATP1 in plasma membrane, Lipin 1 in microsomes and HSL mRNA, while mitochondrial CPT1 was decreased. Exercise decreased plasma free FA level, raised CD36 in plasma membrane and FATP1 in lysate, mitochondrial CPT1 and decreased microsomal Lipin 1 in fructose-fed rats. Conclusions: FRD changed plasma lipids and augmented partitioning of FA to TAG storage in the heart, whereas exercise in FRD rats switched metabolism of FA towards β-oxidation.",
journal = "Archives of Physiology and Biochemistry",
title = "Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats",
volume = "129",
number = "4",
pages = "922-932",
doi = "10.1080/13813455.2021.1886118"
}

Kostić, M., Korićanac, G., Tepavčević, S., Stanišić, J., Romić, S., Ćulafić, T., Ivković, T.,& Stojiljković, M.. (2023). Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats. in Archives of Physiology and Biochemistry, 129(4), 922-932.
https://doi.org/10.1080/13813455.2021.1886118

Kostić M, Korićanac G, Tepavčević S, Stanišić J, Romić S, Ćulafić T, Ivković T, Stojiljković M. Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats. in Archives of Physiology and Biochemistry. 2023;129(4):922-932.
doi:10.1080/13813455.2021.1886118 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Stanišić, Jelena, Romić, Snježana, Ćulafić, Tijana, Ivković, Tamara, Stojiljković, Mojca, "Low-intensity exercise diverts cardiac fatty acid metabolism from triacylglycerol synthesis to beta oxidation in fructose-fed rats" in Archives of Physiology and Biochemistry, 129, no. 4 (2023):922-932,
https://doi.org/10.1080/13813455.2021.1886118 . .

TY  - JOUR
AU  - Prodanović, Radiša
AU  - Kirovski, Danijela
AU  - Vujanac, Ivan
AU  - Đorđević, Ana D.
AU  - Romić, Snježana Đ.
AU  - Pantelić, Marija
AU  - Korićanac, Goran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10356
AB  - A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance, though it is evident that dry cow muscles may contain increased triglyceride content. The current study was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ), glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows. Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin 1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to this disposition.
T2  - Research in Veterinary Science
T1  - Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle
VL  - 150
SP  - 189
EP  - 194
DO  - 10.1016/j.rvsc.2022.04.012
ER  - 

@article{
author = "Prodanović, Radiša and Kirovski, Danijela and Vujanac, Ivan and Đorđević, Ana D. and Romić, Snježana Đ. and Pantelić, Marija and Korićanac, Goran",
year = "2022",
abstract = "A number of alterations have been identified in lipid metabolism within adipose tissue and liver in obesity. Less is known about the capacity of skeletal muscle for the metabolism of fatty acids in obesity-related insulin resistance, though it is evident that dry cow muscles may contain increased triglyceride content. The current study was therefore undertaken to evaluate the skeletal muscle expression of proteins of the fatty acid metabolism in dry cows with different body condition scores (BCS). Sixteen Holstein-Friesian close-up cows were divided into 2 equal groups based on their BCS as optimal (3.25 ≤ BCS ≤ 3.5) and high (4.0 ≤ BCS ≤ 4.25). Blood samples collection and skeletal muscle biopsies were carried out at day 10 before calving. Blood serum was assayed for concentration of resistin using a bovine specific ELISA. Protein expression of insulin receptor beta subunit (IRβ), glucose transporter 4 (GLUT4), fatty acid translocase (FAT/CD36), fatty acid transporter 1 (FATP1), carnitine palmitoyltransferase 1 (CPT1), AMP-acitvated protein kinase (AMPK) and lipin 1 were analyzed in semitendinosus muscle by immunoblot. Resistin differed non-significantly between high-BCS and optimal-BCS cows. Insulin-resistant lipid metabolism in obese cows was paralleled with increased skeletal muscle expression of lipin 1 and GLUT4, and decreased expression of IRβ and FATP1. These data suggest that in obesity-related insulin resistance, metabolic capacity in dry cow skeletal muscles appears to be organized towards the synthesis of signaling intermediates rather than fatty acids oxidation and that altered fatty acid uptake does not contribute to this disposition.",
journal = "Research in Veterinary Science",
title = "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle",
volume = "150",
pages = "189-194",
doi = "10.1016/j.rvsc.2022.04.012"
}

Prodanović, R., Kirovski, D., Vujanac, I., Đorđević, A. D., Romić, S. Đ., Pantelić, M.,& Korićanac, G.. (2022). Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science, 150, 189-194.
https://doi.org/10.1016/j.rvsc.2022.04.012

Prodanović R, Kirovski D, Vujanac I, Đorđević AD, Romić SĐ, Pantelić M, Korićanac G. Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle. in Research in Veterinary Science. 2022;150:189-194.
doi:10.1016/j.rvsc.2022.04.012 .

Prodanović, Radiša, Kirovski, Danijela, Vujanac, Ivan, Đorđević, Ana D., Romić, Snježana Đ., Pantelić, Marija, Korićanac, Goran, "Obesity-related prepartal insulin resistance in dairy cows is associated with increased lipin 1 and decreased FATP 1 expression in skeletal muscle" in Research in Veterinary Science, 150 (2022):189-194,
https://doi.org/10.1016/j.rvsc.2022.04.012 . .

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Popović, Tamara
AU  - Zec, Manja
AU  - Stojiljković, Mojca D.
AU  - Ćulafić, Tijana
AU  - Bošković, Maja
AU  - Korićanac, Goran
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10391
AB  - Walnut consumption mostly has a positive implication for cardiovascular health. Walnut diet effects on the cardiac fatty acid (FA) metabolism of healthy rats and those with fructose diet-induced metabolic burden were analysed. Both walnuts and fructose increased CD36 transporter level and the nuclear content of some/all of Lipin 1/PPARα/PGC-1 complex partners, as well as cytosolic and nuclear FOXO1. However, fructose, independently of walnuts, increased the content of palmitic (PA), oleic, and vaccenic acid (VA), while in walnut-fed rats failed to increase palmitoleic acid (POA) level and the POA/PA ratio, as well as total MUFA content. In opposite, walnuts reduced the level of PA and VA and increased alpha-linolenic, eicosapentaenoic and docosapentaenoic acid level, regardless of fructose. In conclusion, both fructose and walnuts stimulated the uptake and oxidation of FA in the heart, but the walnuts, opposite to fructose, favourably altered cardiac FA profile in healthy and metabolically compromised rats.
T2  - International Journal of Food Sciences and Nutrition
T1  - Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats
SP  - 1
EP  - 14
DO  - 10.1080/09637486.2022.2107186
ER  - 

@article{
author = "Romić, Snježana Đ. and Tepavčević, Snežana and Popović, Tamara and Zec, Manja and Stojiljković, Mojca D. and Ćulafić, Tijana and Bošković, Maja and Korićanac, Goran",
year = "2022",
abstract = "Walnut consumption mostly has a positive implication for cardiovascular health. Walnut diet effects on the cardiac fatty acid (FA) metabolism of healthy rats and those with fructose diet-induced metabolic burden were analysed. Both walnuts and fructose increased CD36 transporter level and the nuclear content of some/all of Lipin 1/PPARα/PGC-1 complex partners, as well as cytosolic and nuclear FOXO1. However, fructose, independently of walnuts, increased the content of palmitic (PA), oleic, and vaccenic acid (VA), while in walnut-fed rats failed to increase palmitoleic acid (POA) level and the POA/PA ratio, as well as total MUFA content. In opposite, walnuts reduced the level of PA and VA and increased alpha-linolenic, eicosapentaenoic and docosapentaenoic acid level, regardless of fructose. In conclusion, both fructose and walnuts stimulated the uptake and oxidation of FA in the heart, but the walnuts, opposite to fructose, favourably altered cardiac FA profile in healthy and metabolically compromised rats.",
journal = "International Journal of Food Sciences and Nutrition",
title = "Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats",
pages = "1-14",
doi = "10.1080/09637486.2022.2107186"
}

Romić, S. Đ., Tepavčević, S., Popović, T., Zec, M., Stojiljković, M. D., Ćulafić, T., Bošković, M.,& Korićanac, G.. (2022). Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats. in International Journal of Food Sciences and Nutrition, 1-14.
https://doi.org/10.1080/09637486.2022.2107186

Romić SĐ, Tepavčević S, Popović T, Zec M, Stojiljković MD, Ćulafić T, Bošković M, Korićanac G. Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats. in International Journal of Food Sciences and Nutrition. 2022;:1-14.
doi:10.1080/09637486.2022.2107186 .

Romić, Snježana Đ., Tepavčević, Snežana, Popović, Tamara, Zec, Manja, Stojiljković, Mojca D., Ćulafić, Tijana, Bošković, Maja, Korićanac, Goran, "Consumption of walnuts suppresses the conversion of palmitic to palmitoleic acid and enhances omega-3 fatty acid metabolism in the heart of fructose-fed rats" in International Journal of Food Sciences and Nutrition (2022):1-14,
https://doi.org/10.1080/09637486.2022.2107186 . .

TY  - JOUR
AU  - Bošković, Maja
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Zec, Manja
AU  - Debeljak-Martačić, Jasmina
AU  - Stanković, Aleksandra
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10485
AB  - Increased fructose consumption has been linked with chronic inflammation and metabolic syndrome (MetS). Activation of the renin-angiotensin system (RAS) and NF-κB have been detected in MetS. Walnuts are a rich source of polyunsaturated omega-3 fatty acids (n-3 PUFA) that were suggested to exert anti-inflammatory effects related to cardio-metabolic health. We hypothesized that walnut supplementation has the capacity to revert unfavorable fructose-rich diet (FRD)-induced activation of cardiac RAS and NF-κB in male rats. Due to the lack of similar studies, we investigated the effects of walnut supplementation (6 weeks) on the expression of four RAS molecules (ACE, ACE2, AT1R, and AT2R) and NF-κB in rat heart after FRD (10% w/v, 9 weeks). In addition, we followed the changes in the n-6/n-3 PUFA ratio in the total pool of heart lipids after both treatments to elucidate the walnut effects on fatty acids in the heart. 36 animals (9 per group) participated in the experiment. FRD significantly increased the ACE protein level in the heart (p < 0.001). Walnut supplementation significantly increased the ACE2 protein level in the heart of FRD (p < 0.001). In addition, walnut supplementation showed a significant main effect on the arachidonic acid/eicosapentaenoic acid ratio (p = 0.004). Walnut supplementation significantly reduced this ratio, in comparison with both, the control group (C vs. FW, p < 0.05) and the FRD group (F vs. FW, p < 0.05). However, walnut treatment failed to revert the significant effect of fructose (p < 0.001) on the elevation of NF-κB protein level. Our results suggest a beneficial effect of walnut supplementation on ACE2 protein level and n-6/n-3 PUFA level in the heart of the animal model of MetS. Such results highlight the approach of omega-3-rich walnut supplementation in the stimulation of endogenous production of favorable molecules in the heart which could be an affordable nutritional treatment formaintenance of cardio-metabolic health.
T2  - Frontiers in Physiology
T1  - Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart
VL  - 13
DO  - 10.3389/fphys.2022.942459
ER  - 

@article{
author = "Bošković, Maja and Živković, Maja and Korićanac, Goran and Tepavčević, Snežana and Zec, Manja and Debeljak-Martačić, Jasmina and Stanković, Aleksandra",
year = "2022",
abstract = "Increased fructose consumption has been linked with chronic inflammation and metabolic syndrome (MetS). Activation of the renin-angiotensin system (RAS) and NF-κB have been detected in MetS. Walnuts are a rich source of polyunsaturated omega-3 fatty acids (n-3 PUFA) that were suggested to exert anti-inflammatory effects related to cardio-metabolic health. We hypothesized that walnut supplementation has the capacity to revert unfavorable fructose-rich diet (FRD)-induced activation of cardiac RAS and NF-κB in male rats. Due to the lack of similar studies, we investigated the effects of walnut supplementation (6 weeks) on the expression of four RAS molecules (ACE, ACE2, AT1R, and AT2R) and NF-κB in rat heart after FRD (10% w/v, 9 weeks). In addition, we followed the changes in the n-6/n-3 PUFA ratio in the total pool of heart lipids after both treatments to elucidate the walnut effects on fatty acids in the heart. 36 animals (9 per group) participated in the experiment. FRD significantly increased the ACE protein level in the heart (p < 0.001). Walnut supplementation significantly increased the ACE2 protein level in the heart of FRD (p < 0.001). In addition, walnut supplementation showed a significant main effect on the arachidonic acid/eicosapentaenoic acid ratio (p = 0.004). Walnut supplementation significantly reduced this ratio, in comparison with both, the control group (C vs. FW, p < 0.05) and the FRD group (F vs. FW, p < 0.05). However, walnut treatment failed to revert the significant effect of fructose (p < 0.001) on the elevation of NF-κB protein level. Our results suggest a beneficial effect of walnut supplementation on ACE2 protein level and n-6/n-3 PUFA level in the heart of the animal model of MetS. Such results highlight the approach of omega-3-rich walnut supplementation in the stimulation of endogenous production of favorable molecules in the heart which could be an affordable nutritional treatment formaintenance of cardio-metabolic health.",
journal = "Frontiers in Physiology",
title = "Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart",
volume = "13",
doi = "10.3389/fphys.2022.942459"
}

Bošković, M., Živković, M., Korićanac, G., Tepavčević, S., Zec, M., Debeljak-Martačić, J.,& Stanković, A.. (2022). Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart. in Frontiers in Physiology, 13.
https://doi.org/10.3389/fphys.2022.942459

Bošković M, Živković M, Korićanac G, Tepavčević S, Zec M, Debeljak-Martačić J, Stanković A. Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart. in Frontiers in Physiology. 2022;13.
doi:10.3389/fphys.2022.942459 .

Bošković, Maja, Živković, Maja, Korićanac, Goran, Tepavčević, Snežana, Zec, Manja, Debeljak-Martačić, Jasmina, Stanković, Aleksandra, "Walnut supplementation after fructose-rich diet is associated with a beneficial fatty acid ratio and increased ACE2 expression in the rat heart" in Frontiers in Physiology, 13 (2022),
https://doi.org/10.3389/fphys.2022.942459 . .

TY  - CONF
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stanišić, Jelena
AU  - Ivković, Tamara
AU  - Pantelić, Marija
AU  - Stojiljković, Mojca D.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11009
AB  - Excessive dietary fructose consumption in parallel with limited physical activity contributes to the global increase in prevalence of metabolic disorders. Metabolic syndrome represents a collection of cardiometabolic risk factors that includes obesity, insulin resistance, hypertension, and dyslipidemia, and it is undoubtedly linked to increased risk for two global maladies, type 2 diabetes, and cardiovascular diseases. Fructose-rich diet is accompanied by the development of insulin resistance in the heart, and it could change the use of cardiac energy substrates towards increased fatty acid (FA) uptake, and catabolism. Exercise may be beneficial in prevention and treatment of the metabolic syndrome. The aim of this study was to analyse the impact of low-intensity exercise on protein expression of nuclear transcription factors involved in regulating FA β- oxidation in a heart of fructose fed rats. Male Wistar rats were divided into control group, and two groups that received 10% fructose for 9 weeks, one which was sedentary and one which was additionally exposed to low intensity exercise. The protein expression of important transcriptional regulators of fatty acid β-oxidation PPARα, and FOXO1, and coregulators Lipin1, PGC-1, and SIRT1 are analyzed in cardiac lysate and/or nuclear fraction by Western blot. Gene expression of ACADL, the enzyme that catalyzes the initial step of mitochondrial β-oxidation, was quantified by real-time PCR. Fructose-rich diet decreased nuclear PPARα compared to control. Exercise increased nuclear PPARα, nuclear FOXO1, lysate PGC1, and nuclear Lipin1 in fructose-fed rats compared to sedentary fructose-fed rats. Exercise increased lysate PPARα, lysate and nuclear FOXO1, lysate PGC1, lysate and nuclear SIRT1, and nuclear Lipin1 in fructose-fed rats compared to control. In conclusion, running at low intensity is accompanied by increased expression of key regulators of fatty acid oxidation. The results indicate that exercise achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1.
PB  - Belgrade : Faculty of Chemistry : Serbian Biochemical Society
C3  - Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
T1  - Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats
SP  - 86
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11009
ER  - 

@conference{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Ćulafić, Tijana and Romić, Snježana Đ. and Stanišić, Jelena and Ivković, Tamara and Pantelić, Marija and Stojiljković, Mojca D.",
year = "2022",
abstract = "Excessive dietary fructose consumption in parallel with limited physical activity contributes to the global increase in prevalence of metabolic disorders. Metabolic syndrome represents a collection of cardiometabolic risk factors that includes obesity, insulin resistance, hypertension, and dyslipidemia, and it is undoubtedly linked to increased risk for two global maladies, type 2 diabetes, and cardiovascular diseases. Fructose-rich diet is accompanied by the development of insulin resistance in the heart, and it could change the use of cardiac energy substrates towards increased fatty acid (FA) uptake, and catabolism. Exercise may be beneficial in prevention and treatment of the metabolic syndrome. The aim of this study was to analyse the impact of low-intensity exercise on protein expression of nuclear transcription factors involved in regulating FA β- oxidation in a heart of fructose fed rats. Male Wistar rats were divided into control group, and two groups that received 10% fructose for 9 weeks, one which was sedentary and one which was additionally exposed to low intensity exercise. The protein expression of important transcriptional regulators of fatty acid β-oxidation PPARα, and FOXO1, and coregulators Lipin1, PGC-1, and SIRT1 are analyzed in cardiac lysate and/or nuclear fraction by Western blot. Gene expression of ACADL, the enzyme that catalyzes the initial step of mitochondrial β-oxidation, was quantified by real-time PCR. Fructose-rich diet decreased nuclear PPARα compared to control. Exercise increased nuclear PPARα, nuclear FOXO1, lysate PGC1, and nuclear Lipin1 in fructose-fed rats compared to sedentary fructose-fed rats. Exercise increased lysate PPARα, lysate and nuclear FOXO1, lysate PGC1, lysate and nuclear SIRT1, and nuclear Lipin1 in fructose-fed rats compared to control. In conclusion, running at low intensity is accompanied by increased expression of key regulators of fatty acid oxidation. The results indicate that exercise achieves its effect by increasing the nuclear content of PPARα, Lipin1, and FOXO1.",
publisher = "Belgrade : Faculty of Chemistry : Serbian Biochemical Society",
journal = "Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia",
title = "Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats",
pages = "86",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11009"
}

Kostić, M., Korićanac, G., Tepavčević, S., Ćulafić, T., Romić, S. Đ., Stanišić, J., Ivković, T., Pantelić, M.,& Stojiljković, M. D.. (2022). Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
Belgrade : Faculty of Chemistry : Serbian Biochemical Society., 86.
https://hdl.handle.net/21.15107/rcub_vinar_11009

Kostić M, Korićanac G, Tepavčević S, Ćulafić T, Romić SĐ, Stanišić J, Ivković T, Pantelić M, Stojiljković MD. Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia. 2022;:86.
https://hdl.handle.net/21.15107/rcub_vinar_11009 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Ćulafić, Tijana, Romić, Snježana Đ., Stanišić, Jelena, Ivković, Tamara, Pantelić, Marija, Stojiljković, Mojca D., "Improvement of lipid metabolism regulation by low-intensity exercise in fructose-fed rats" in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia (2022):86,
https://hdl.handle.net/21.15107/rcub_vinar_11009 .

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Ivković, Tamara
AU  - Romić, Snježana Đ.
AU  - Zec, Manja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Korićanac, Goran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9103
AB  - Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.
T2  - International Journal of Food Sciences and Nutrition
T1  - Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2
VL  - 72
IS  - 3
SP  - 324
EP  - 334
DO  - 10.1080/09637486.2020.1796931
ER  - 

@article{
author = "Stanišić, Jelena and Ivković, Tamara and Romić, Snježana Đ. and Zec, Manja and Ćulafić, Tijana and Stojiljković, Mojca D. and Korićanac, Goran",
year = "2021",
abstract = "Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.",
journal = "International Journal of Food Sciences and Nutrition",
title = "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2",
volume = "72",
number = "3",
pages = "324-334",
doi = "10.1080/09637486.2020.1796931"
}

Stanišić, J., Ivković, T., Romić, S. Đ., Zec, M., Ćulafić, T., Stojiljković, M. D.,& Korićanac, G.. (2021). Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences and Nutrition, 72(3), 324-334.
https://doi.org/10.1080/09637486.2020.1796931

Stanišić J, Ivković T, Romić SĐ, Zec M, Ćulafić T, Stojiljković MD, Korićanac G. Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences and Nutrition. 2021;72(3):324-334.
doi:10.1080/09637486.2020.1796931 .

Stanišić, Jelena, Ivković, Tamara, Romić, Snježana Đ., Zec, Manja, Ćulafić, Tijana, Stojiljković, Mojca D., Korićanac, Goran, "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2" in International Journal of Food Sciences and Nutrition, 72, no. 3 (2021):324-334,
https://doi.org/10.1080/09637486.2020.1796931 . .

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Ivković, Tamara
AU  - Romić, Snježana Đ.
AU  - Zec, Manja
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Korićanac, Goran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9110
AB  - Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.
T2  - International Journal of Food Sciences and Nutrition
T1  - Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2
VL  - 72
IS  - 3
SP  - 324
EP  - 334
DO  - 10.1080/09637486.2020.1796931
ER  - 

@article{
author = "Stanišić, Jelena and Ivković, Tamara and Romić, Snježana Đ. and Zec, Manja and Ćulafić, Tijana and Stojiljković, Mojca D. and Korićanac, Goran",
year = "2021",
abstract = "Consumption of walnuts is beneficial for cardiovascular health. To study walnut effects on proteins involved in vascular tone regulation, control and fructose-fed rats were subjected to walnut diet for 6 weeks. In contrast with increased energy intake and body mass gain, aortic protein level of L-type calcium channel alpha subunit was decreased and the level of SUR2B subunit of ATP-sensitive K + channel was increased in healthy rats subjected to walnuts, together with improved Akt phosphorylation. Upon the walnut diet in rats subjected to fructose overload, the rise in energy intake and body mass gain, was followed by an increase in blood insulin. Although SUR2B level was elevated, the level of sodium-calcium exchanger NCX1 and inducible nitric oxide synthase were reduced and increased, respectively. In summary, walnut consumption was accompanied with moderate beneficial vascular effect in healthy rats, while an effect of walnut in rats with metabolic disturbances was rather controversial.",
journal = "International Journal of Food Sciences and Nutrition",
title = "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2",
volume = "72",
number = "3",
pages = "324-334",
doi = "10.1080/09637486.2020.1796931"
}

Stanišić, J., Ivković, T., Romić, S. Đ., Zec, M., Ćulafić, T., Stojiljković, M. D.,& Korićanac, G.. (2021). Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences and Nutrition, 72(3), 324-334.
https://doi.org/10.1080/09637486.2020.1796931

Stanišić J, Ivković T, Romić SĐ, Zec M, Ćulafić T, Stojiljković MD, Korićanac G. Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2. in International Journal of Food Sciences and Nutrition. 2021;72(3):324-334.
doi:10.1080/09637486.2020.1796931 .

Stanišić, Jelena, Ivković, Tamara, Romić, Snježana Đ., Zec, Manja, Ćulafić, Tijana, Stojiljković, Mojca D., Korićanac, Goran, "Beneficial effect of walnuts on vascular tone is associated with Akt signalling, voltage-dependent calcium channel LTCC and ATP-sensitive potassium channel Kv1.2" in International Journal of Food Sciences and Nutrition, 72, no. 3 (2021):324-334,
https://doi.org/10.1080/09637486.2020.1796931 . .

TY  - JOUR
AU  - Bošković, Maja
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanišić, Jelena
AU  - Zec, Manja
AU  - Krga, Irena S.
AU  - Stanković, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9809
AB  - The benefits of walnut (Juglans regia) consumption for metabolic health are known, but the molecular background underlying their putative antioxidant and anti-inflammatory/immunomodulatory effects is underexplored. We assessed that walnut supplementation (6 weeks) reverted unfavorable changes of the SIRT1/FoxO3a/MnSOD/catalase axis in the heart induced by fructose-rich diet (FRD). Intriguingly, Nox4 was increased by both FRD and walnut supplementation. FRD increased the cytosolic fraction and decreased the nuclear fraction of the uniquely elucidated ChREBP in the heart. The ChREBP nuclear fraction was decreased in control rats subjected to walnuts. In addition, walnut consumption was associated with a reduction in systolic BP in FRD and a decrease in fatty acid AA/EPA and AA/DHA ratios in plasma. In summary, the protective effect of walnut supplementation was detected in male rats following the fructose-induced decrease in antioxidative/anti-inflammatory capacity of cardiac tissue and increase in plasma predictors of low-grade inflammation. The current results provide a novel insight into the relationship between nutrients, cellular energy homeostasis, and the modulators of inflammatory/immune response in metabolic syndrome, emphasizing the heart and highlighting a track for translation into nutrition and dietary therapeutic approaches against metabolic disease.
T2  - Oxidative Medicine and Cellular Longevity
T1  - Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet
VL  - 2021
SP  - e5543025
DO  - 10.1155/2021/5543025
ER  - 

@article{
author = "Bošković, Maja and Živković, Maja and Korićanac, Goran and Stanišić, Jelena and Zec, Manja and Krga, Irena S. and Stanković, Aleksandra",
year = "2021",
abstract = "The benefits of walnut (Juglans regia) consumption for metabolic health are known, but the molecular background underlying their putative antioxidant and anti-inflammatory/immunomodulatory effects is underexplored. We assessed that walnut supplementation (6 weeks) reverted unfavorable changes of the SIRT1/FoxO3a/MnSOD/catalase axis in the heart induced by fructose-rich diet (FRD). Intriguingly, Nox4 was increased by both FRD and walnut supplementation. FRD increased the cytosolic fraction and decreased the nuclear fraction of the uniquely elucidated ChREBP in the heart. The ChREBP nuclear fraction was decreased in control rats subjected to walnuts. In addition, walnut consumption was associated with a reduction in systolic BP in FRD and a decrease in fatty acid AA/EPA and AA/DHA ratios in plasma. In summary, the protective effect of walnut supplementation was detected in male rats following the fructose-induced decrease in antioxidative/anti-inflammatory capacity of cardiac tissue and increase in plasma predictors of low-grade inflammation. The current results provide a novel insight into the relationship between nutrients, cellular energy homeostasis, and the modulators of inflammatory/immune response in metabolic syndrome, emphasizing the heart and highlighting a track for translation into nutrition and dietary therapeutic approaches against metabolic disease.",
journal = "Oxidative Medicine and Cellular Longevity",
title = "Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet",
volume = "2021",
pages = "e5543025",
doi = "10.1155/2021/5543025"
}

Bošković, M., Živković, M., Korićanac, G., Stanišić, J., Zec, M., Krga, I. S.,& Stanković, A.. (2021). Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet. in Oxidative Medicine and Cellular Longevity, 2021, e5543025.
https://doi.org/10.1155/2021/5543025

Bošković M, Živković M, Korićanac G, Stanišić J, Zec M, Krga IS, Stanković A. Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet. in Oxidative Medicine and Cellular Longevity. 2021;2021:e5543025.
doi:10.1155/2021/5543025 .

Bošković, Maja, Živković, Maja, Korićanac, Goran, Stanišić, Jelena, Zec, Manja, Krga, Irena S., Stanković, Aleksandra, "Walnut Supplementation Restores the SIRT1-FoxO3a-MnSOD/Catalase Axis in the Heart, Promotes an Anti-Inflammatory Fatty Acid Profile in Plasma, and Lowers Blood Pressure on Fructose-Rich Diet" in Oxidative Medicine and Cellular Longevity, 2021 (2021):e5543025,
https://doi.org/10.1155/2021/5543025 . .

TY  - JOUR
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Ivković, Tamara
AU  - Tepavčević, Snežana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9919
AB  - We previously reported that low-intensity exercise prevented cardiac insulin resistance induced by a fructose-rich diet (FRD). To examine whether low-intensity exercise could prevent the disturbances of key molecules of cardiac glucose metabolism induced by FRD in male and ovariectomized (ovx) female rats, animals were exposed to 10% fructose solution (SF) or underwent both fructose diet and exercise (EF). Exercise prevented a decrease in cardiac GSK-3β phosphorylation induced by FRD in males (p <.001 vs. SF). It also prevented a decrease in PFK-2 phosphorylation in ovx females (p <.001 vs. SF) and increased the expression of PFK-2 in males (p <.05 vs. control). Exercise did not prevent a decrease in plasma membrane GLUT1 and GLUT4 levels in ovx females on FRD. The only effect of exercise on glucose transporters that could be indicated as beneficial is an augmented GLUT4 protein expression in males (p <.05 vs. control). Obtained results suggest that low-intensity exercise prevents harmful effects of FRD towards cardiac glycogenesis in males and glycolysis in ovx females. Practical applications: Low-intensity exercise, equivalent to brisk walking, was able to prevent disturbances in cardiac glycolysis regulation in ovx female and the glycogen synthesis pathway in male rats. In terms of human health, although molecular mechanisms of beneficial effects of exercise on cardiac glucose metabolism vary between genders, low-intensity running may be a useful non-pharmacological approach in the prevention of cardiac metabolic disorders in both men and postmenopausal women. © 2021 Wiley Periodicals LLC.
T2  - Journal of Food Biochemistry
T1  - The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet
VL  - 45
IS  - 10
DO  - 10.1111/jfbc.13930
ER  - 

@article{
author = "Stanišić, Jelena and Korićanac, Goran and Ćulafić, Tijana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Ivković, Tamara and Tepavčević, Snežana",
year = "2021",
abstract = "We previously reported that low-intensity exercise prevented cardiac insulin resistance induced by a fructose-rich diet (FRD). To examine whether low-intensity exercise could prevent the disturbances of key molecules of cardiac glucose metabolism induced by FRD in male and ovariectomized (ovx) female rats, animals were exposed to 10% fructose solution (SF) or underwent both fructose diet and exercise (EF). Exercise prevented a decrease in cardiac GSK-3β phosphorylation induced by FRD in males (p <.001 vs. SF). It also prevented a decrease in PFK-2 phosphorylation in ovx females (p <.001 vs. SF) and increased the expression of PFK-2 in males (p <.05 vs. control). Exercise did not prevent a decrease in plasma membrane GLUT1 and GLUT4 levels in ovx females on FRD. The only effect of exercise on glucose transporters that could be indicated as beneficial is an augmented GLUT4 protein expression in males (p <.05 vs. control). Obtained results suggest that low-intensity exercise prevents harmful effects of FRD towards cardiac glycogenesis in males and glycolysis in ovx females. Practical applications: Low-intensity exercise, equivalent to brisk walking, was able to prevent disturbances in cardiac glycolysis regulation in ovx female and the glycogen synthesis pathway in male rats. In terms of human health, although molecular mechanisms of beneficial effects of exercise on cardiac glucose metabolism vary between genders, low-intensity running may be a useful non-pharmacological approach in the prevention of cardiac metabolic disorders in both men and postmenopausal women. © 2021 Wiley Periodicals LLC.",
journal = "Journal of Food Biochemistry",
title = "The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet",
volume = "45",
number = "10",
doi = "10.1111/jfbc.13930"
}

Stanišić, J., Korićanac, G., Ćulafić, T., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Ivković, T.,& Tepavčević, S.. (2021). The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet. in Journal of Food Biochemistry, 45(10).
https://doi.org/10.1111/jfbc.13930

Stanišić J, Korićanac G, Ćulafić T, Romić SĐ, Stojiljković MD, Kostić M, Ivković T, Tepavčević S. The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet. in Journal of Food Biochemistry. 2021;45(10).
doi:10.1111/jfbc.13930 .

Stanišić, Jelena, Korićanac, Goran, Ćulafić, Tijana, Romić, Snježana Đ., Stojiljković, Mojca D., Kostić, Milan, Ivković, Tamara, Tepavčević, Snežana, "The effects of low‐intensity exercise on cardiac glycogenesis and glycolysis in male and ovariectomized female rats on a fructose‐rich diet" in Journal of Food Biochemistry, 45, no. 10 (2021),
https://doi.org/10.1111/jfbc.13930 . .

TY  - JOUR
AU  - Ivković, Tamara
AU  - Čulafić, Tijana
AU  - Tepavčević, Snežana
AU  - Romić, Snježana Đ.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10054
AB  - Context The evidence on potential cross-talk of vitamin D and insulin in the regulation of cardiac metabolism is very scanty.Objective Cholecalciferol was administered to male Wistar rats for six weeks to study its effects on cardiac glucose metabolism regulation.Materials and methods An expression, phosphorylation and/or subcellular localisation of insulin signalling molecules, glucose transport and metabolism key proteins were studied.Results Circulating non-esterified fatty acids (NEFA) level was lower after cholecalciferol administration. Cholecalciferol decreased cardiac insulin receptor substrate 1 Ser307 phosphorylation, while insulin-stimulated Akt Thr308 phosphorylation was increased. Cardiac 6-phosphofructo-2-kinase protein, hexokinase 2 mRNA level and insulin-stimulated glycogen synthase kinase 3β Ser9 phosphorylation were also increased. Finally, FOXO1 transcription factor cytosolic level was reduced.Conclusion Vitamin D-related improvement of insulin signalling and insulin regulation of glucose metabolism in the rat heart is accompanied by the decrease of blood NEFA level and dysregulation of cardiac FOXO1 signalling.
T2  - Archives of Physiology and Biochemistry
T1  - Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart
SP  - 1
EP  - 9
DO  - 10.1080/13813455.2021.2001020
ER  - 

@article{
author = "Ivković, Tamara and Čulafić, Tijana and Tepavčević, Snežana and Romić, Snježana Đ. and Stojiljković, Mojca D. and Kostić, Milan and Stanišić, Jelena and Korićanac, Goran",
year = "2021",
abstract = "Context The evidence on potential cross-talk of vitamin D and insulin in the regulation of cardiac metabolism is very scanty.Objective Cholecalciferol was administered to male Wistar rats for six weeks to study its effects on cardiac glucose metabolism regulation.Materials and methods An expression, phosphorylation and/or subcellular localisation of insulin signalling molecules, glucose transport and metabolism key proteins were studied.Results Circulating non-esterified fatty acids (NEFA) level was lower after cholecalciferol administration. Cholecalciferol decreased cardiac insulin receptor substrate 1 Ser307 phosphorylation, while insulin-stimulated Akt Thr308 phosphorylation was increased. Cardiac 6-phosphofructo-2-kinase protein, hexokinase 2 mRNA level and insulin-stimulated glycogen synthase kinase 3β Ser9 phosphorylation were also increased. Finally, FOXO1 transcription factor cytosolic level was reduced.Conclusion Vitamin D-related improvement of insulin signalling and insulin regulation of glucose metabolism in the rat heart is accompanied by the decrease of blood NEFA level and dysregulation of cardiac FOXO1 signalling.",
journal = "Archives of Physiology and Biochemistry",
title = "Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart",
pages = "1-9",
doi = "10.1080/13813455.2021.2001020"
}

Ivković, T., Čulafić, T., Tepavčević, S., Romić, S. Đ., Stojiljković, M. D., Kostić, M., Stanišić, J.,& Korićanac, G.. (2021). Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart. in Archives of Physiology and Biochemistry, 1-9.
https://doi.org/10.1080/13813455.2021.2001020

Ivković T, Čulafić T, Tepavčević S, Romić SĐ, Stojiljković MD, Kostić M, Stanišić J, Korićanac G. Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart. in Archives of Physiology and Biochemistry. 2021;:1-9.
doi:10.1080/13813455.2021.2001020 .

Ivković, Tamara, Čulafić, Tijana, Tepavčević, Snežana, Romić, Snježana Đ., Stojiljković, Mojca D., Kostić, Milan, Stanišić, Jelena, Korićanac, Goran, "Cholecalciferol ameliorates insulin signalling and insulin regulation of enzymes involved in glucose metabolism in the rat heart" in Archives of Physiology and Biochemistry (2021):1-9,
https://doi.org/10.1080/13813455.2021.2001020 . .

TY  - JOUR
AU  - Romić, Snježana Đ.
AU  - Đorđević, Ana
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Stojiljković, Mojca D.
AU  - Bursać, Biljana
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Gligorovska, Ljupka
AU  - Korićanac, Goran
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8849
AB  - Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.
T2  - Food and Function
T1  - Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats
VL  - 11
IS  - 2
SP  - 1455
EP  - 1466
DO  - 10.1039/C9FO02306B
ER  - 

@article{
author = "Romić, Snježana Đ. and Đorđević, Ana and Tepavčević, Snežana and Ćulafić, Tijana and Stojiljković, Mojca D. and Bursać, Biljana and Stanišić, Jelena and Kostić, Milan and Gligorovska, Ljupka and Korićanac, Goran",
year = "2020",
abstract = "Both a diet rich in fructose and chronic stress exposure induce metabolic and cardiovascular disturbances. The aim of this study was to examine the effects of the fructose-rich diet and chronic stress, separately and in combination, on insulin signaling and molecules regulating glycogen synthesis and ion transport in the heart, and to reveal whether these effects coincide with changes in glucocorticoid receptor (GR) activation. Male Wistar rats were subjected to 10% fructose in drinking water and/or to chronic unpredictable stress for 9 weeks. Protein expression and/or phosphorylation of the insulin receptor (IR), protein tyrosine phosphatase 1B, insulin receptor substrate 1 (IRS1), protein kinase B (Akt), extracellular signal-regulated kinase 1/2 (ERK1/2), glycogen synthase kinase-3β (GSK-3β) and Na+/K+-ATPase α-subunits in cardiac tissue were analyzed by western blot. GR distribution between cytosolic and nuclear fractions was also analyzed. The fructose-rich diet decreased the level of pERK1/2 (Thr202/Tyr204) and pGSK-3β (Ser9) independently of stress, while chronic stress increased the IRS1 content and prevented the fructose diet-induced decrease of the pAkt (Ser473) level. The fructose-rich diet in combination with chronic stress reduced the protein content of cardiac IR and attenuated IRS1 upregulation. Separate treatments increased the protein content of Na+/K+-ATPase α1- and α2-subunits, while after combined treatment the α2 content was at the control level and the α1 content was lower than the control level. The effect of combined treatment on cardiac IR and α2-subunit expression could be mediated by increased GR nuclear accumulation. Our study provides new insights into the effects of chronic stress and a combination of the fructose diet and chronic stress on the studied molecules in the heart.",
journal = "Food and Function",
title = "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats",
volume = "11",
number = "2",
pages = "1455-1466",
doi = "10.1039/C9FO02306B"
}

Romić, S. Đ., Đorđević, A., Tepavčević, S., Ćulafić, T., Stojiljković, M. D., Bursać, B., Stanišić, J., Kostić, M., Gligorovska, L.,& Korićanac, G.. (2020). Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats. in Food and Function, 11(2), 1455-1466.
https://doi.org/10.1039/C9FO02306B

Romić SĐ, Đorđević A, Tepavčević S, Ćulafić T, Stojiljković MD, Bursać B, Stanišić J, Kostić M, Gligorovska L, Korićanac G. Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats. in Food and Function. 2020;11(2):1455-1466.
doi:10.1039/C9FO02306B .

Romić, Snježana Đ., Đorđević, Ana, Tepavčević, Snežana, Ćulafić, Tijana, Stojiljković, Mojca D., Bursać, Biljana, Stanišić, Jelena, Kostić, Milan, Gligorovska, Ljupka, Korićanac, Goran, "Effects of a fructose-rich diet and chronic stress on insulin signaling and regulation of glycogen synthase kinase-3 beta and the sodium–potassium pump in the hearts of male rats" in Food and Function, 11, no. 2 (2020):1455-1466,
https://doi.org/10.1039/C9FO02306B . .

TY  - JOUR
AU  - Zec, Manja M.
AU  - Krga, Irena S.
AU  - Takić, Marija M.
AU  - Debeljak-Martačić, Jasmina D.
AU  - Korićanac, Goran
AU  - Ranković, Slavica G.
AU  - Popović, Tamara B.
AU  - Pantelić, Marija
AU  - Glibetić, Maria D.
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9731
AB  - Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.
PB  - Amer Chemical Soc, Washington
T2  - ACS Omega
T1  - Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats
VL  - 5
IS  - 43
SP  - 28136
EP  - 28145
DO  - 10.1021/acsomega.0c03784
ER  - 

@article{
author = "Zec, Manja M. and Krga, Irena S. and Takić, Marija M. and Debeljak-Martačić, Jasmina D. and Korićanac, Goran and Ranković, Slavica G. and Popović, Tamara B. and Pantelić, Marija and Glibetić, Maria D.",
year = "2020",
abstract = "Increased dietary, blood, and tissue n-6/n-3 fatty acid ratios are associated with obesity and metabolic syndrome. Due to Westernized dietary patterns, the increasing n-6/n-3 ratio is of growing concern worldwide, and dietary strategies aimed at its lowering are of public health importance. Walnuts are rich in dietary fats, and their consumption promotes cardiometabolic health. This study aimed to examine the effect of 6-week walnut consumption on tissue-specific n-6/n-3 ratio and fatty acid metabolic conversion in fructose-fed rats with a cluster of metabolic disorders. Male Wistar rats were fed a standard diet with or without 10% fructose in drinking water for 9 weeks. Diets of half of the animals were then supplemented with walnuts (2.4 g/day) for 6 weeks, upon which fatty acid profiles were determined in plasma, liver, adipose tissue, and kidney total lipids. Results showed that walnuts induced significant decreases in the n-6/n-3 content of total lipid pool in plasma and examined tissues, irrespective of metabolic burden. Walnut intervention decreased plasma and liver palmitoleic/palmitic, arachidonic/linoleic, and docosahexaenoic/alpha-linolenic acid ratios. It also modulated individual fatty acid levels by reducing arachidonic and palmitic acid and increasing alpha-linolenic, eicosapentaenoic, and docosapentaenoic acid in plasma and most tissues. Our study demonstrated that 6-week consumption of walnuts favorably modulated n-6/n-3 plasma and tissue ratio in male Wistar rats regardless of high-fructose feeding, underscoring the promising potential of walnuts in both prevention and treatment of the metabolic syndrome.",
publisher = "Amer Chemical Soc, Washington",
journal = "ACS Omega",
title = "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats",
volume = "5",
number = "43",
pages = "28136-28145",
doi = "10.1021/acsomega.0c03784"
}

Zec, M. M., Krga, I. S., Takić, M. M., Debeljak-Martačić, J. D., Korićanac, G., Ranković, S. G., Popović, T. B., Pantelić, M.,& Glibetić, M. D.. (2020). Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega
Amer Chemical Soc, Washington., 5(43), 28136-28145.
https://doi.org/10.1021/acsomega.0c03784

Zec MM, Krga IS, Takić MM, Debeljak-Martačić JD, Korićanac G, Ranković SG, Popović TB, Pantelić M, Glibetić MD. Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats. in ACS Omega. 2020;5(43):28136-28145.
doi:10.1021/acsomega.0c03784 .

Zec, Manja M., Krga, Irena S., Takić, Marija M., Debeljak-Martačić, Jasmina D., Korićanac, Goran, Ranković, Slavica G., Popović, Tamara B., Pantelić, Marija, Glibetić, Maria D., "Walnut Consumption Induces Tissue-Specific Omega-6/Omega-3 Decrease in High-Fructose-Fed Wistar Rats" in ACS Omega, 5, no. 43 (2020):28136-28145,
https://doi.org/10.1021/acsomega.0c03784 . .

TY  - JOUR
AU  - Bošković, Maja
AU  - Bundalo, Maja M.
AU  - Živković, Maja
AU  - Stanišić, Jelena
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7984
AB  - Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.
T2  - Journal of Functional Foods
T1  - Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats
VL  - 52
SP  - 690
EP  - 698
DO  - 10.1016/j.jff.2018.11.053
ER  - 

@article{
author = "Bošković, Maja and Bundalo, Maja M. and Živković, Maja and Stanišić, Jelena and Kostić, Milan and Korićanac, Goran and Stanković, Aleksandra",
year = "2019",
abstract = "Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.",
journal = "Journal of Functional Foods",
title = "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats",
volume = "52",
pages = "690-698",
doi = "10.1016/j.jff.2018.11.053"
}

Bošković, M., Bundalo, M. M., Živković, M., Stanišić, J., Kostić, M., Korićanac, G.,& Stanković, A.. (2019). Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats. in Journal of Functional Foods, 52, 690-698.
https://doi.org/10.1016/j.jff.2018.11.053

Bošković M, Bundalo MM, Živković M, Stanišić J, Kostić M, Korićanac G, Stanković A. Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats. in Journal of Functional Foods. 2019;52:690-698.
doi:10.1016/j.jff.2018.11.053 .

Bošković, Maja, Bundalo, Maja M., Živković, Maja, Stanišić, Jelena, Kostić, Milan, Korićanac, Goran, Stanković, Aleksandra, "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats" in Journal of Functional Foods, 52 (2019):690-698,
https://doi.org/10.1016/j.jff.2018.11.053 . .

TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7804
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 

@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods.",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}

Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655

Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55.
doi:10.1111/jpn.12655 .

Pantelić, Marija, Jovanović, Ljubomir J., Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Ćulafić, Tijana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" in Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 . .

TY  - JOUR
AU  - Pantelić, Marija
AU  - Jovanović, Ljubomir J.
AU  - Prodanović, Radiša
AU  - Vujanac, Ivan
AU  - Đurić, Miloje
AU  - Ćulafić, Tijana
AU  - Vranješ-Đurić, Sanja
AU  - Korićanac, Goran
AU  - Kirovski, Danijela
PY  - 2018
UR  - http://doi.wiley.com/10.1111/jpn.12655
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7835
AB  - Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH
T2  - Journal of Animal Physiology and Animal Nutrition
T1  - The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows
VL  - 102
IS  - 1
SP  - 41
EP  - 55
DO  - 10.1111/jpn.12655
ER  - 

@article{
author = "Pantelić, Marija and Jovanović, Ljubomir J. and Prodanović, Radiša and Vujanac, Ivan and Đurić, Miloje and Ćulafić, Tijana and Vranješ-Đurić, Sanja and Korićanac, Goran and Kirovski, Danijela",
year = "2018",
abstract = "Thirty days before expected time of parturition, 20 Holstein cows were divided into −Cr and +Cr groups. From day 25 before parturition (BP) up to day 30 after parturition (AP), +Cr cows received 10 mg of Cr (chromium-enriched yeast) daily. Muscle and adipose tissue samples were taken at days −30, −10, +7 and +10 related to parturition, when body condition score (BCS) was also determined. Hepatic tissue samples were taken at days −10 and +7. Tissue samples were used for determination of the insulin signalling pathway protein expressions. Intravenous glucose tolerance test (IVGTT) was performed at days −28, −7, +10 and +30. Milk yield was recorded during first 14 weeks AP. Milk composition was obtained at days 7 and 28 AP. At day 10 BP, protein content of β-subunit of insulin receptor (IRβ) was significantly higher (p ˂ 0.05) in muscle, and phosphorylation of insulin receptor substrate 1 at serine 307 (pIRS-1 Ser307) was significantly lower (p ˂ 0.05) in hepatic tissue of +Cr group. After parturition, pIRS-1 Ser307 was significantly lower in muscle tissue at days 7 and 28 (p ˂ 0.05 and p ˂ 0.001, respectively), while phosphorylation of Akt at serine 473 (pAkt Ser473) was significantly higher (p ˂ 0.01) in hepatic tissue at day 7 AP in +Cr group. Chromium had opposite effect on insulin kinetics during IVGTTs obtained BP and AP. Insulin secretion was significantly reduced at day 7 BP and significantly enhanced at day 10 AP, when NEFA concentration was also significantly increased. Milk yield and ECM value were depressed in +Cr group. DMI and BCS were significantly enhanced in +Cr group at day 7 BP. In conclusion, chromium modulates insulin signalling pathway in dairy cows, but targeted signalling molecules are different in antepartal then post-partal period, probably due to duration of exposure to chromium and different energy status between those periods. © 2017 Blackwell Verlag GmbH",
journal = "Journal of Animal Physiology and Animal Nutrition",
title = "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows",
volume = "102",
number = "1",
pages = "41-55",
doi = "10.1111/jpn.12655"
}

Pantelić, M., Jovanović, L. J., Prodanović, R., Vujanac, I., Đurić, M., Ćulafić, T., Vranješ-Đurić, S., Korićanac, G.,& Kirovski, D.. (2018). The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition, 102(1), 41-55.
https://doi.org/10.1111/jpn.12655

Pantelić M, Jovanović LJ, Prodanović R, Vujanac I, Đurić M, Ćulafić T, Vranješ-Đurić S, Korićanac G, Kirovski D. The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows. in Journal of Animal Physiology and Animal Nutrition. 2018;102(1):41-55.
doi:10.1111/jpn.12655 .

Pantelić, Marija, Jovanović, Ljubomir J., Prodanović, Radiša, Vujanac, Ivan, Đurić, Miloje, Ćulafić, Tijana, Vranješ-Đurić, Sanja, Korićanac, Goran, Kirovski, Danijela, "The impact of the chromium supplementation on insulin signalling pathway in different tissues and milk yield in dairy cows" in Journal of Animal Physiology and Animal Nutrition, 102, no. 1 (2018):41-55,
https://doi.org/10.1111/jpn.12655 . .

TY  - CONF
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Stojiljković, Mirjana
AU  - Ćulafić, Tijana
AU  - Kostić, Mirjana M.
AU  - Romić, Snježana Đ.
AU  - Pantelić, Marija
AU  - Tepavčević, Snežana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7920
C3  - FEBS OPEN BIO
T1  - Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats
VL  - 8
IS  - Supplement 1
SP  - 224
DO  - 10.1002/2211-5463.12453
ER  - 

@conference{
author = "Stanišić, Jelena and Korićanac, Goran and Stojiljković, Mirjana and Ćulafić, Tijana and Kostić, Mirjana M. and Romić, Snježana Đ. and Pantelić, Marija and Tepavčević, Snežana",
year = "2018",
journal = "FEBS OPEN BIO",
title = "Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats",
volume = "8",
number = "Supplement 1",
pages = "224",
doi = "10.1002/2211-5463.12453"
}

Stanišić, J., Korićanac, G., Stojiljković, M., Ćulafić, T., Kostić, M. M., Romić, S. Đ., Pantelić, M.,& Tepavčević, S.. (2018). Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats. in FEBS OPEN BIO, 8(Supplement 1), 224.
https://doi.org/10.1002/2211-5463.12453

Stanišić J, Korićanac G, Stojiljković M, Ćulafić T, Kostić MM, Romić SĐ, Pantelić M, Tepavčević S. Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats. in FEBS OPEN BIO. 2018;8(Supplement 1):224.
doi:10.1002/2211-5463.12453 .

Stanišić, Jelena, Korićanac, Goran, Stojiljković, Mirjana, Ćulafić, Tijana, Kostić, Mirjana M., Romić, Snježana Đ., Pantelić, Marija, Tepavčević, Snežana, "Low intensity exercise prevents disturbances in insulin regulation of alpha subunits of Na plus /K plus -ATPase in the heart of fructose-fed female rats" in FEBS OPEN BIO, 8, no. Supplement 1 (2018):224,
https://doi.org/10.1002/2211-5463.12453 . .

TY  - CONF
AU  - Kostić, Milan
AU  - Korićanac, Goran
AU  - Tepavčević, Snežana
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Stanišić, Jelena
AU  - Pantelić, Marija
AU  - Stojiljković, Mirjana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7922
C3  - FEBS OPEN BIO
T1  - Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet
VL  - 8
IS  - Supplement 1
SP  - 223
DO  - 10.1002/2211-5463.12453
ER  - 

@conference{
author = "Kostić, Milan and Korićanac, Goran and Tepavčević, Snežana and Ćulafić, Tijana and Romić, Snježana Đ. and Stanišić, Jelena and Pantelić, Marija and Stojiljković, Mirjana",
year = "2018",
journal = "FEBS OPEN BIO",
title = "Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet",
volume = "8",
number = "Supplement 1",
pages = "223",
doi = "10.1002/2211-5463.12453"
}

Kostić, M., Korićanac, G., Tepavčević, S., Ćulafić, T., Romić, S. Đ., Stanišić, J., Pantelić, M.,& Stojiljković, M.. (2018). Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet. in FEBS OPEN BIO, 8(Supplement 1), 223.
https://doi.org/10.1002/2211-5463.12453

Kostić M, Korićanac G, Tepavčević S, Ćulafić T, Romić SĐ, Stanišić J, Pantelić M, Stojiljković M. Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet. in FEBS OPEN BIO. 2018;8(Supplement 1):223.
doi:10.1002/2211-5463.12453 .

Kostić, Milan, Korićanac, Goran, Tepavčević, Snežana, Ćulafić, Tijana, Romić, Snježana Đ., Stanišić, Jelena, Pantelić, Marija, Stojiljković, Mirjana, "Moderate physical activity alters cardiac lipid metabolism of male rats on high fructose diet" in FEBS OPEN BIO, 8, no. Supplement 1 (2018):223,
https://doi.org/10.1002/2211-5463.12453 . .

TY  - CONF
AU  - Stanišić, Jelena
AU  - Korićanac, Goran
AU  - Stojiljković, Mojca D.
AU  - Ćulafić, Tijana
AU  - Romić, Snježana Đ.
AU  - Kostić, Milan
AU  - Pantelić, M.
AU  - Tepavčević, Snežana
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7945
C3  - Atherosclerosis
T1  - Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise
VL  - 275
SP  - e197
DO  - 10.1016/j.atherosclerosis.2018.06.607
ER  - 

@conference{
author = "Stanišić, Jelena and Korićanac, Goran and Stojiljković, Mojca D. and Ćulafić, Tijana and Romić, Snježana Đ. and Kostić, Milan and Pantelić, M. and Tepavčević, Snežana",
year = "2018",
journal = "Atherosclerosis",
title = "Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise",
volume = "275",
pages = "e197",
doi = "10.1016/j.atherosclerosis.2018.06.607"
}

Stanišić, J., Korićanac, G., Stojiljković, M. D., Ćulafić, T., Romić, S. Đ., Kostić, M., Pantelić, M.,& Tepavčević, S.. (2018). Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise. in Atherosclerosis, 275, e197.
https://doi.org/10.1016/j.atherosclerosis.2018.06.607

Stanišić J, Korićanac G, Stojiljković MD, Ćulafić T, Romić SĐ, Kostić M, Pantelić M, Tepavčević S. Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise. in Atherosclerosis. 2018;275:e197.
doi:10.1016/j.atherosclerosis.2018.06.607 .

Stanišić, Jelena, Korićanac, Goran, Stojiljković, Mojca D., Ćulafić, Tijana, Romić, Snježana Đ., Kostić, Milan, Pantelić, M., Tepavčević, Snežana, "Disturbances in cardiac insulin signaling and nitric oxide synthase in ovariectomized rats on fructose diet can be prevented by low intensity exercise" in Atherosclerosis, 275 (2018):e197,
https://doi.org/10.1016/j.atherosclerosis.2018.06.607 . .

TY  - CONF
AU  - Bošković, Maja
AU  - Bundalo, Maja M.
AU  - Stojiljković, Mojca D.
AU  - Kostić, Milan
AU  - Živković, Maja
AU  - Korićanac, Goran
AU  - Stanković, Aleksandra
AU  - Životić, Ivan
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7179
C3  - Atherosclerosis
T1  - Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress
VL  - 263
SP  - E192
EP  - E192
DO  - 10.1016/j.atherosclerosis.2017.06.616
ER  - 

@conference{
author = "Bošković, Maja and Bundalo, Maja M. and Stojiljković, Mojca D. and Kostić, Milan and Živković, Maja and Korićanac, Goran and Stanković, Aleksandra and Životić, Ivan",
year = "2017",
journal = "Atherosclerosis",
title = "Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress",
volume = "263",
pages = "E192-E192",
doi = "10.1016/j.atherosclerosis.2017.06.616"
}

Bošković, M., Bundalo, M. M., Stojiljković, M. D., Kostić, M., Živković, M., Korićanac, G., Stanković, A.,& Životić, I.. (2017). Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress. in Atherosclerosis, 263, E192-E192.
https://doi.org/10.1016/j.atherosclerosis.2017.06.616

Bošković M, Bundalo MM, Stojiljković MD, Kostić M, Živković M, Korićanac G, Stanković A, Životić I. Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress. in Atherosclerosis. 2017;263:E192-E192.
doi:10.1016/j.atherosclerosis.2017.06.616 .

Bošković, Maja, Bundalo, Maja M., Stojiljković, Mojca D., Kostić, Milan, Živković, Maja, Korićanac, Goran, Stanković, Aleksandra, Životić, Ivan, "Estradiol Protects Ovariectomized Female Rats Against Fructose-Rich Diet Induced Oxidative Stress" in Atherosclerosis, 263 (2017):E192-E192,
https://doi.org/10.1016/j.atherosclerosis.2017.06.616 . .

TY  - JOUR
AU  - Bundalo, Maja M.
AU  - Romić, Snježana Đ.
AU  - Tepavčević, Snežana
AU  - Stojiljković, Mojca D.
AU  - Stanković, Aleksandra
AU  - Živković, Maja
AU  - Korićanac, Goran
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1726
AB  - Increased intake of fructose in humans and laboratory animals is demonstrated to be a risk factor for development of metabolic disorders (insulin resistance, metabolic syndrome, type 2 diabetes) and cardiovascular diseases. On the other hand, estradiol is emphasized as a cardioprotective agent. The main goal of this review is to summarize recent findings on damaging cardiac effects of fructose-rich diet in females, mostly experimental animals, and to evaluate protective capacity of estradiol. Published results of our and other research groups indicate mostly detrimental effects of fructose-rich diet on cardiac insulin signaling molecules, glucose and fatty acid metabolism, nitric oxide production and ion transport, as well as renin-angiotensin system and inflammation. Some of these processes are involved in cardiac insulin signal transmission, others are regulated by insulin or have an influence on insulin action. Administration of estradiol to ovariectomized female rats, exposed to increased intake of fructose, was mostly beneficial to the heart, but sometimes it was ineffective or even detrimental, depending on the particular processes. We believe that these data, carefully translated to human population, could be useful for clinicians dealing with postmenopausal women susceptible to metabolic diseases and hormone replacement therapy.
T2  - European Journal of Pharmacology
T1  - Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?
VL  - 811
SP  - 141
EP  - 147
DO  - 10.1016/j.ejphar.2017.06.003
ER  - 

@article{
author = "Bundalo, Maja M. and Romić, Snježana Đ. and Tepavčević, Snežana and Stojiljković, Mojca D. and Stanković, Aleksandra and Živković, Maja and Korićanac, Goran",
year = "2017",
abstract = "Increased intake of fructose in humans and laboratory animals is demonstrated to be a risk factor for development of metabolic disorders (insulin resistance, metabolic syndrome, type 2 diabetes) and cardiovascular diseases. On the other hand, estradiol is emphasized as a cardioprotective agent. The main goal of this review is to summarize recent findings on damaging cardiac effects of fructose-rich diet in females, mostly experimental animals, and to evaluate protective capacity of estradiol. Published results of our and other research groups indicate mostly detrimental effects of fructose-rich diet on cardiac insulin signaling molecules, glucose and fatty acid metabolism, nitric oxide production and ion transport, as well as renin-angiotensin system and inflammation. Some of these processes are involved in cardiac insulin signal transmission, others are regulated by insulin or have an influence on insulin action. Administration of estradiol to ovariectomized female rats, exposed to increased intake of fructose, was mostly beneficial to the heart, but sometimes it was ineffective or even detrimental, depending on the particular processes. We believe that these data, carefully translated to human population, could be useful for clinicians dealing with postmenopausal women susceptible to metabolic diseases and hormone replacement therapy.",
journal = "European Journal of Pharmacology",
title = "Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?",
volume = "811",
pages = "141-147",
doi = "10.1016/j.ejphar.2017.06.003"
}

Bundalo, M. M., Romić, S. Đ., Tepavčević, S., Stojiljković, M. D., Stanković, A., Živković, M.,& Korićanac, G.. (2017). Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?. in European Journal of Pharmacology, 811, 141-147.
https://doi.org/10.1016/j.ejphar.2017.06.003

Bundalo MM, Romić SĐ, Tepavčević S, Stojiljković MD, Stanković A, Živković M, Korićanac G. Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?. in European Journal of Pharmacology. 2017;811:141-147.
doi:10.1016/j.ejphar.2017.06.003 .

Bundalo, Maja M., Romić, Snježana Đ., Tepavčević, Snežana, Stojiljković, Mojca D., Stanković, Aleksandra, Živković, Maja, Korićanac, Goran, "Fructose-rich diet and insulin action in female rat heart: Estradiol friend or foe?" in European Journal of Pharmacology, 811 (2017):141-147,
https://doi.org/10.1016/j.ejphar.2017.06.003 . .