Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats
Само за регистроване кориснике
2019
Аутори
Bošković, MajaBundalo, Maja M.
Živković, Maja
Stanišić, Jelena
Kostić, Milan
Korićanac, Goran
Stanković, Aleksandra
Чланак у часопису (Објављена верзија)
,
© 2018 Published by Elsevier Ltd.
Метаподаци
Приказ свих података о документуАпстракт
Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The pr...oposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.
Кључне речи:
Metabolic syndrome / Fructose / Estradiol / Heart / miRNAИзвор:
Journal of Functional Foods, 2019, 52, 690-698Финансирање / пројекти:
- Улога стероидних хормона у неуроендокриној адаптацији на стрес и патофизиологији метаболичког синдрома - молекуларни механизми и клиничке импликације (RS-41009)
- Генетска основа хуманих васкуларних и инфламаторних болести (RS-175085)
DOI: 10.1016/j.jff.2018.11.053
ISSN: 1756-4646
WoS: 000455691800073
Scopus: 2-s2.0-85057766748
Институција/група
VinčaTY - JOUR AU - Bošković, Maja AU - Bundalo, Maja M. AU - Živković, Maja AU - Stanišić, Jelena AU - Kostić, Milan AU - Korićanac, Goran AU - Stanković, Aleksandra PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7984 AB - Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu. T2 - Journal of Functional Foods T1 - Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats VL - 52 SP - 690 EP - 698 DO - 10.1016/j.jff.2018.11.053 ER -
@article{ author = "Bošković, Maja and Bundalo, Maja M. and Živković, Maja and Stanišić, Jelena and Kostić, Milan and Korićanac, Goran and Stanković, Aleksandra", year = "2019", abstract = "Harmful effects of fructose-rich diet (FRD) were predominantly observed in males, suggesting protective effects of estrogens. Little is known about AMPK/sirtuin-1 (SIRT1)/forkhead box O3 (FoxO3a)/manganese superoxide dismutase (MnSOD)/catalase signaling in the heart in state of metabolic syndrome and oxidative stress induced by fructose over-consumption. We investigated the effect of 10% FRD on expression of AMPK-SIRT1-FoxO3a-MnSOD/catalase axis in myocardium and potentially beneficial effect of 17β-estradiol replacement. The expression of NADPH oxidase 4 (Nox4) and miRNA-155, unfavorable regulators of this axis, were also investigated. FRD significantly increased AMPK and decreased FoxO3a activity, decreased SIRT1, MnSOD and Nox4 protein expression while E2 reverted these changes, except for Nox4, and increased catalase protein level. E2 diminished Nox4 and MnSOD mRNA level in FRD ovariectomized rats. These results suggest independent response of AMPK and SIRT to FRD treatment. The proposed signaling in the heart should be further investigated in the prooxidative and antioxidative milieu.", journal = "Journal of Functional Foods", title = "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats", volume = "52", pages = "690-698", doi = "10.1016/j.jff.2018.11.053" }
Bošković, M., Bundalo, M. M., Živković, M., Stanišić, J., Kostić, M., Korićanac, G.,& Stanković, A.. (2019). Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats. in Journal of Functional Foods, 52, 690-698. https://doi.org/10.1016/j.jff.2018.11.053
Bošković M, Bundalo MM, Živković M, Stanišić J, Kostić M, Korićanac G, Stanković A. Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats. in Journal of Functional Foods. 2019;52:690-698. doi:10.1016/j.jff.2018.11.053 .
Bošković, Maja, Bundalo, Maja M., Živković, Maja, Stanišić, Jelena, Kostić, Milan, Korićanac, Goran, Stanković, Aleksandra, "Estradiol ameliorates antioxidant axis SIRT1-FoxO3a-MnSOD/catalase in the heart of fructose-fed ovariectomized rats" in Journal of Functional Foods, 52 (2019):690-698, https://doi.org/10.1016/j.jff.2018.11.053 . .