TY  - JOUR
AU  - Milosavljević, Slobodan
AU  - Đorđević, Iris
AU  - Mandić, Boris
AU  - Tešević, Vele V.
AU  - Stanković, Miroslava
AU  - Todorović, Nina
AU  - Novaković, Miroslav M.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10107
AB  - In continuation of our study on Cotinus coggygria from Serbia, 10 known flavonoids (1-10) were isolated from the methylene chloride/ methanol extract of the heartwood. They were tested for in vitro protective effect against chromosome aberrations in peripheral human lymphocytes, using the cytokinesis-block micronucleus assay. All tested compounds (in minimal doses of 1 μg/mL) exerted a beneficial effect by decreasing DNA damage of human lymphocytes in the range of 24.2% to 54.5%, better than the radio protectant control, amifostine. Functional groups, such as 3′,4′-dihydroxyphenyl (catechol), 5-OH, 3-OH, and 4-keto in flavonoids (3-keto in aurones), which play a key role in antioxidant activity, are proposed to be responsible for the DNA protective activity of the tested compounds.
T2  - Natural Product Communications
T1  - Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA
VL  - 16
IS  - 12
SP  - 1
EP  - 8
DO  - 10.1177/1934578X211067289
ER  - 

@article{
author = "Milosavljević, Slobodan and Đorđević, Iris and Mandić, Boris and Tešević, Vele V. and Stanković, Miroslava and Todorović, Nina and Novaković, Miroslav M.",
year = "2021",
abstract = "In continuation of our study on Cotinus coggygria from Serbia, 10 known flavonoids (1-10) were isolated from the methylene chloride/ methanol extract of the heartwood. They were tested for in vitro protective effect against chromosome aberrations in peripheral human lymphocytes, using the cytokinesis-block micronucleus assay. All tested compounds (in minimal doses of 1 μg/mL) exerted a beneficial effect by decreasing DNA damage of human lymphocytes in the range of 24.2% to 54.5%, better than the radio protectant control, amifostine. Functional groups, such as 3′,4′-dihydroxyphenyl (catechol), 5-OH, 3-OH, and 4-keto in flavonoids (3-keto in aurones), which play a key role in antioxidant activity, are proposed to be responsible for the DNA protective activity of the tested compounds.",
journal = "Natural Product Communications",
title = "Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA",
volume = "16",
number = "12",
pages = "1-8",
doi = "10.1177/1934578X211067289"
}

Milosavljević, S., Đorđević, I., Mandić, B., Tešević, V. V., Stanković, M., Todorović, N.,& Novaković, M. M.. (2021). Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA. in Natural Product Communications, 16(12), 1-8.
https://doi.org/10.1177/1934578X211067289

Milosavljević S, Đorđević I, Mandić B, Tešević VV, Stanković M, Todorović N, Novaković MM. Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA. in Natural Product Communications. 2021;16(12):1-8.
doi:10.1177/1934578X211067289 .

Milosavljević, Slobodan, Đorđević, Iris, Mandić, Boris, Tešević, Vele V., Stanković, Miroslava, Todorović, Nina, Novaković, Miroslav M., "Flavonoids of the Heartwood of Cotinus coggygria Scop. Showing Protective Effect on Human Lymphocyte DNA" in Natural Product Communications, 16, no. 12 (2021):1-8,
https://doi.org/10.1177/1934578X211067289 . .

TY  - JOUR
AU  - Zlatanović, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Zrnzevic, Ivana
AU  - Đorđević, Aleksandra
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1466
AB  - This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.
T2  - Journal of the Serbian Chemical Society
T1  - Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract
VL  - 82
IS  - 2
SP  - 141
EP  - 150
DO  - 10.2298/JSC161019014Z
ER  - 

@article{
author = "Zlatanović, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Zrnzevic, Ivana and Đorđević, Aleksandra and Stojanović, Gordana",
year = "2017",
abstract = "This paper reports for the first time the effect of an acetone extract of Umbilicaria crustulosa on the micronucleus distribution of human lymphocytes, and on the cholinesterase activity and antioxidant activity by the cupric ion reducing antioxidant capacity (CUPRAC) method. Additionally, the total phenolic compounds (TPC) and the antioxidant properties were estimated via DPPH, ABTS and TRP assays. Moreover, the antibacterial activity against two Gram-positive and three Gram-negative bacteria were determined. Acetone extract of U. crustulosa at concentration of 1 and 2 mu g mL(-1) decreased a frequency of micronuclei (MN) by 10.8 and 16.8 %, respectively, acting more or slightly less than the synthetic protector amifostine (AMF, WR-2721, 11.4 %, at concentration of 1 mu g mL(-1)). The tested extract did not inhibit cholinesterase activity nor did it exhibit activity toward the examined bacteria. The extract reduced the concentration of DPPH and ABTS radicals by 88.7 and 96.2 %, respectively. Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.6197+/-0.0166 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 19.7641+/-1.6546 mu g trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 350.4188 +/-14.587 mu g gallic acid equivalents (GAE) per mg of dry extract. The results of the present study showed that U. crustulosa acetone extract is a promising candidate for in vivo experiments considering its antioxidant activity and protective effect on human lymphocytes.",
journal = "Journal of the Serbian Chemical Society",
title = "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract",
volume = "82",
number = "2",
pages = "141-150",
doi = "10.2298/JSC161019014Z"
}

Zlatanović, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Zrnzevic, I., Đorđević, A.,& Stojanović, G.. (2017). Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society, 82(2), 141-150.
https://doi.org/10.2298/JSC161019014Z

Zlatanović I, Stanković M, Stankov Jovanović V, Mitić V, Zrnzevic I, Đorđević A, Stojanović G. Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract. in Journal of the Serbian Chemical Society. 2017;82(2):141-150.
doi:10.2298/JSC161019014Z .

Zlatanović, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Zrnzevic, Ivana, Đorđević, Aleksandra, Stojanović, Gordana, "Biological activities of Umbilicaria crustulosa (Ach.) Frey acetone extract" in Journal of the Serbian Chemical Society, 82, no. 2 (2017):141-150,
https://doi.org/10.2298/JSC161019014Z . .

TY  - JOUR
AU  - Zrnzevic, Ivana
AU  - Stanković, Miroslava
AU  - Stankov Jovanović, Vesna
AU  - Mitić, Violeta
AU  - Đorđević, Aleksandra
AU  - Zlatanović, Ivana
AU  - Stojanović, Gordana
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1656
AB  - In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.
T2  - EXCLI Journal
T1  - Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity
VL  - 16
SP  - 679
EP  - 687
DO  - 10.17179/excli2017-301
ER  - 

@article{
author = "Zrnzevic, Ivana and Stanković, Miroslava and Stankov Jovanović, Vesna and Mitić, Violeta and Đorđević, Aleksandra and Zlatanović, Ivana and Stojanović, Gordana",
year = "2017",
abstract = "In the present investigation, effects of Ramalina capitata acetone extract on micronucleus distribution on human lymphocytes, on cholinesterase activity and antioxidant activity (by the CUPRAC method) were examined, for the first time as well as its HPLC profile. Additionally, total phenolic compounds (TPC), antioxidant properties (estimated via DPPH, ABTS and TRP assays) and antibacterial activity were determined. The predominant phenolic compounds in this extract were evernic, everninic and obtusatic acids. Acetone extract of R. capitata at concentration of 2 mu g mL(-1) decreased a frequency of micronuclei (MN) for 14.8 %. The extract reduces the concentration of DPPH and ABTS radicals for 21.2 and 36.1 % (respectively). Values for total reducing power (TRP) and cupric reducing capacity (CUPRAC) were 0.4624 +/- 0.1064 mu g ascorbic acid equivalents (AAE) per mg of dry extract, and 6.1176 +/- 0.2964 mu g Trolox equivalents (TE) per mg of dry extract, respectively. The total phenol content was 670.6376 +/- 66.554 mu g galic acid equivalents (GAE) per mg of dry extract. Tested extract at concentration of 2 mg mL(-1) exhibited inhibition effect (5.2 %) on pooled human serum cholinesterase. The antimicrobial assay showed that acetone extract had inhibition effect towards Gram-positive strains. The results of manifested antioxidant activity, reducing the number of micronuclei in human lymphocytes, and antibacterial activity recommends R. capitata extract for further in vivo studies.",
journal = "EXCLI Journal",
title = "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity",
volume = "16",
pages = "679-687",
doi = "10.17179/excli2017-301"
}

Zrnzevic, I., Stanković, M., Stankov Jovanović, V., Mitić, V., Đorđević, A., Zlatanović, I.,& Stojanović, G.. (2017). Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal, 16, 679-687.
https://doi.org/10.17179/excli2017-301

Zrnzevic I, Stanković M, Stankov Jovanović V, Mitić V, Đorđević A, Zlatanović I, Stojanović G. Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity. in EXCLI Journal. 2017;16:679-687.
doi:10.17179/excli2017-301 .

Zrnzevic, Ivana, Stanković, Miroslava, Stankov Jovanović, Vesna, Mitić, Violeta, Đorđević, Aleksandra, Zlatanović, Ivana, Stojanović, Gordana, "Ramalina Capitata (Ach.) Nyl. Acetone Extract: Hplc Analysis, Genotoxicity, Cholinesterase, Antioxidant and Antibacterial Activity" in EXCLI Journal, 16 (2017):679-687,
https://doi.org/10.17179/excli2017-301 . .

TY  - JOUR
AU  - Kostić, Danijela A.
AU  - Stanković, Miroslava
AU  - Milovanović, Vesna M.
AU  - Milovanović, N.
AU  - Stojanović, Gordana S.
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8009
AB  - The contents of phenols, flavonoids anthocyans and antioxidant activity of the water (WE), methanol (ME) and methanol-water (MWE) (50/50, v/v) extracts of M. sylvestris fresh and dry petals were determined. Further, for all above mentioned extracts, in vitro effect of the frequency of micronuclei (MN) on peripheral human lymphocytes was evaluated using cytochalasin-B micronucleus (CBMN) assay. Total phenol content (mg of gallic acid equivalent/g of petals) varied from 13.20 (WE) to 29.20 (ME) of fresh petals and for dry petals from 80 (WE) to 142 (ME). The content of flavonoids (mg of catechin equivalent /g of petals) ranged from 47.50 (WE) to 101.20 (ME) of fresh petals and for dry petals from 439 (WE) to 814 (ME). The anthocyans content (mg of cyanidin-3-O-glucoside/g of petals) ranged from 0.40 (WE) to 0.72 (ME) of fresh petals and for dry petals from 3.13 (WE) to 6.16 (ME). All extracts showed high antioxidant activity (more than 67%). ME of fresh petals showed a greater decrease in the frequency of MN (from 23.9 to 29.3%) in all tested concentrations (0.5; 1.0 and 2.0 µg/ml) compared to standard radio of protectant to Amifostine (20.8%). © 2017, Scibulcom Ltd. All rights reserved.
T2  - Oxidation Communications
T1  - Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte
VL  - 40
IS  - 2
SP  - 602
EP  - 612
UR  - https://hdl.handle.net/21.15107/rcub_vinar_8009
ER  - 

@article{
author = "Kostić, Danijela A. and Stanković, Miroslava and Milovanović, Vesna M. and Milovanović, N. and Stojanović, Gordana S.",
year = "2017",
abstract = "The contents of phenols, flavonoids anthocyans and antioxidant activity of the water (WE), methanol (ME) and methanol-water (MWE) (50/50, v/v) extracts of M. sylvestris fresh and dry petals were determined. Further, for all above mentioned extracts, in vitro effect of the frequency of micronuclei (MN) on peripheral human lymphocytes was evaluated using cytochalasin-B micronucleus (CBMN) assay. Total phenol content (mg of gallic acid equivalent/g of petals) varied from 13.20 (WE) to 29.20 (ME) of fresh petals and for dry petals from 80 (WE) to 142 (ME). The content of flavonoids (mg of catechin equivalent /g of petals) ranged from 47.50 (WE) to 101.20 (ME) of fresh petals and for dry petals from 439 (WE) to 814 (ME). The anthocyans content (mg of cyanidin-3-O-glucoside/g of petals) ranged from 0.40 (WE) to 0.72 (ME) of fresh petals and for dry petals from 3.13 (WE) to 6.16 (ME). All extracts showed high antioxidant activity (more than 67%). ME of fresh petals showed a greater decrease in the frequency of MN (from 23.9 to 29.3%) in all tested concentrations (0.5; 1.0 and 2.0 µg/ml) compared to standard radio of protectant to Amifostine (20.8%). © 2017, Scibulcom Ltd. All rights reserved.",
journal = "Oxidation Communications",
title = "Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte",
volume = "40",
number = "2",
pages = "602-612",
url = "https://hdl.handle.net/21.15107/rcub_vinar_8009"
}

Kostić, D. A., Stanković, M., Milovanović, V. M., Milovanović, N.,& Stojanović, G. S.. (2017). Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte. in Oxidation Communications, 40(2), 602-612.
https://hdl.handle.net/21.15107/rcub_vinar_8009

Kostić DA, Stanković M, Milovanović VM, Milovanović N, Stojanović GS. Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte. in Oxidation Communications. 2017;40(2):602-612.
https://hdl.handle.net/21.15107/rcub_vinar_8009 .

Kostić, Danijela A., Stanković, Miroslava, Milovanović, Vesna M., Milovanović, N., Stojanović, Gordana S., "Malva sylvestris l. Petals extracts: Composition, antioxidant activity and effect on micronucleus distribution in human lymphocyte" in Oxidation Communications, 40, no. 2 (2017):602-612,
https://hdl.handle.net/21.15107/rcub_vinar_8009 .

TY  - JOUR
AU  - Stojanović, Gordana
AU  - Stanković, Miroslava
AU  - Stojanovic, Igor Z.
AU  - Palic, Ivan
AU  - Milovanović, Vesna
AU  - Rancic, Sofija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5969
AB  - Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.
T2  - Natural Product Communications
T1  - Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes
VL  - 9
IS  - 4
SP  - 503
EP  - 504
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5969
ER  - 

@article{
author = "Stojanović, Gordana and Stanković, Miroslava and Stojanovic, Igor Z. and Palic, Ivan and Milovanović, Vesna and Rancic, Sofija",
year = "2014",
abstract = "Three lichen secondary metabolites atranorin (1), evernic acid (2), and usnic acid (3), were evaluated for their in vitro clastogenic and antiproliferative effects on human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay at concentrations of 2 mu g/mL, 4 mu g/mL and 6 mu g/mL of final culture solution. The frequency of micronucleus (MN) was scored in binucleated cells, and cytokinesis-block proliferation index (CBPI) was calculated. Among the tested compounds, 3 exhibited the most prominent effect decreasing the frequency of MN in the range of 42.5% - 48.9%, that is about double of the positive control amifostin WR-2721 that reduces MN frequency for 22.0%. The effect of evernic acid was approximately equal to action of amifostin (23.2% -32.9%). Atranorin at concentrations of 2 mu g/mL and 4 mu g/mL decreasing the frequency of MN only for 11.1% and 1.8%, while in concentration of 6 mu g/mL increases the frequency of MN for 9.6 %. The comparable CBPI values of the investigated compounds and control suggested that they did not show a statistically significant inhibitory effect on lymphocyte cell proliferation at applied concentrations.",
journal = "Natural Product Communications",
title = "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes",
volume = "9",
number = "4",
pages = "503-504",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5969"
}

Stojanović, G., Stanković, M., Stojanovic, I. Z., Palic, I., Milovanović, V.,& Rancic, S.. (2014). Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications, 9(4), 503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969

Stojanović G, Stanković M, Stojanovic IZ, Palic I, Milovanović V, Rancic S. Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes. in Natural Product Communications. 2014;9(4):503-504.
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

Stojanović, Gordana, Stanković, Miroslava, Stojanovic, Igor Z., Palic, Ivan, Milovanović, Vesna, Rancic, Sofija, "Clastogenic Effect of Atranorin, Evernic acid, and Usnic Acid on Human Lymphocytes" in Natural Product Communications, 9, no. 4 (2014):503-504,
https://hdl.handle.net/21.15107/rcub_vinar_5969 .

TY  - JOUR
AU  - Stojanovic, Igor Z.
AU  - Stanković, Miroslava
AU  - Jovanovic, Olga
AU  - Petrovic, Goran
AU  - Smelcerovic, Andrija
AU  - Stojanović, Gordana
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5302
AB  - Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.
T2  - Natural Product Communications
T1  - Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes
VL  - 8
IS  - 1
SP  - 109
EP  - 112
UR  - https://hdl.handle.net/21.15107/rcub_vinar_5302
ER  - 

@article{
author = "Stojanovic, Igor Z. and Stanković, Miroslava and Jovanovic, Olga and Petrovic, Goran and Smelcerovic, Andrija and Stojanović, Gordana",
year = "2013",
abstract = "Three lichen depsidones, physodalic acid (1), physodic acid (2), and 3-hydroxy physodic acid (3), were isolated from Hypogymnia physodes diethyl ether extract using column chromatography, and their structures determined by comparing their UV, H-1 and C-13 NMR spectroscopic and MS data with those given in the literature, as well as with data computed by CHEM draw ultra 11 software. The contents of 1, 2 and 3 were determined in the methanol (ME), acetone (AE), and diethyl ether (EE) extracts using reversed-phase high performance liquid chromatography with photodiode array detection. The extracts, isolated depsidones 1-3 and EE fraction F23 (consisting of 90% 2 and 3, in the ratio 5.5: 1) were evaluated for their in vitro effects on chromosome aberrations in peripheral human lymphocytes using the cytochalasin-B blocked micronucleus (CBMN) assay in doses of 1 mu g/mL and 2 mu g/mL of final culture solution. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. It was found that 1, 2, 3, F23, and EE at 1.0 mu g/mL exerted a beneficial effect on lymphocyte cells giving a significant decrease of the frequency of MN in comparison with the positive control Amifostin WR-2721. Among the tested depsidones at a concentration of 1 mu g/mL, 3 exhibited the most prominent effect decreasing the frequency of MN by 30.3%, followed by 2 (28.2%) and 1 (22.0%). The extracts were less effective than the isolated depsidones.",
journal = "Natural Product Communications",
title = "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes",
volume = "8",
number = "1",
pages = "109-112",
url = "https://hdl.handle.net/21.15107/rcub_vinar_5302"
}

Stojanovic, I. Z., Stanković, M., Jovanovic, O., Petrovic, G., Smelcerovic, A.,& Stojanović, G.. (2013). Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications, 8(1), 109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302

Stojanovic IZ, Stanković M, Jovanovic O, Petrovic G, Smelcerovic A, Stojanović G. Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes. in Natural Product Communications. 2013;8(1):109-112.
https://hdl.handle.net/21.15107/rcub_vinar_5302 .

Stojanovic, Igor Z., Stanković, Miroslava, Jovanovic, Olga, Petrovic, Goran, Smelcerovic, Andrija, Stojanović, Gordana, "Effect of Hypogymnia physodes Extracts and their Depsidones on Micronucleus Distribution in Human Lymphocytes" in Natural Product Communications, 8, no. 1 (2013):109-112,
https://hdl.handle.net/21.15107/rcub_vinar_5302 .

TY  - JOUR
AU  - Godevac, Dejan
AU  - Tešević, Vele V.
AU  - Vajs, Vlatka
AU  - Milosavljević, Slobodan
AU  - Zdunic, Gordana
AU  - Ordevic, Boban
AU  - Stanković, Miroslava
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4923
AB  - On the basis of LC/UV/MS analysis, 35 compounds from the extracts of seeds of black, red, and white currants (2 cultivars of each) were identified. Black currants cultivars contained protocatechuic acid and p-hydroxybenzoic acid, and traces of nitril containing phenolic acids. The presence of synapoyl glucoside was characteristic for cv. Malling Jewel. Sesquiterpenoid glycosides and carboxymethylindol glycosides were present mainly in white and red currant cultivars. Blackcurrant seeds contained higher amounts of flavonoids, especially rutin, isoquercetin, and taxifolin. The currant seed extracts were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. The frequency of MN was scored in binucleated cells, and the cultivars of black currants showed the best antioxidant potential. At a concentration of only 0.17 mg/mL, extract of the seed cv. Malling Jewel effected a decrease in the frequency of MN of 60% compared with control cell cultures. Practical Application: Our results provide evidence of protective effects of currant seed extracts and isolated pure compounds on cytogenetic damages in human lymphocytes. Thus, currant seed extracts could exert beneficial effects in quite a few diseases, for many of the biological actions have been attributed to their antioxidant properties.
T2  - Journal of Food Science
T1  - Chemical Composition of Currant Seed Extracts and Their Protective Effect on Human Lymphocytes DNA
VL  - 77
IS  - 7
SP  - C779
EP  - C783
DO  - 10.1111/j.1750-3841.2012.02756.x
ER  - 

@article{
author = "Godevac, Dejan and Tešević, Vele V. and Vajs, Vlatka and Milosavljević, Slobodan and Zdunic, Gordana and Ordevic, Boban and Stanković, Miroslava",
year = "2012",
abstract = "On the basis of LC/UV/MS analysis, 35 compounds from the extracts of seeds of black, red, and white currants (2 cultivars of each) were identified. Black currants cultivars contained protocatechuic acid and p-hydroxybenzoic acid, and traces of nitril containing phenolic acids. The presence of synapoyl glucoside was characteristic for cv. Malling Jewel. Sesquiterpenoid glycosides and carboxymethylindol glycosides were present mainly in white and red currant cultivars. Blackcurrant seeds contained higher amounts of flavonoids, especially rutin, isoquercetin, and taxifolin. The currant seed extracts were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. The frequency of MN was scored in binucleated cells, and the cultivars of black currants showed the best antioxidant potential. At a concentration of only 0.17 mg/mL, extract of the seed cv. Malling Jewel effected a decrease in the frequency of MN of 60% compared with control cell cultures. Practical Application: Our results provide evidence of protective effects of currant seed extracts and isolated pure compounds on cytogenetic damages in human lymphocytes. Thus, currant seed extracts could exert beneficial effects in quite a few diseases, for many of the biological actions have been attributed to their antioxidant properties.",
journal = "Journal of Food Science",
title = "Chemical Composition of Currant Seed Extracts and Their Protective Effect on Human Lymphocytes DNA",
volume = "77",
number = "7",
pages = "C779-C783",
doi = "10.1111/j.1750-3841.2012.02756.x"
}

Godevac, D., Tešević, V. V., Vajs, V., Milosavljević, S., Zdunic, G., Ordevic, B.,& Stanković, M.. (2012). Chemical Composition of Currant Seed Extracts and Their Protective Effect on Human Lymphocytes DNA. in Journal of Food Science, 77(7), C779-C783.
https://doi.org/10.1111/j.1750-3841.2012.02756.x

Godevac D, Tešević VV, Vajs V, Milosavljević S, Zdunic G, Ordevic B, Stanković M. Chemical Composition of Currant Seed Extracts and Their Protective Effect on Human Lymphocytes DNA. in Journal of Food Science. 2012;77(7):C779-C783.
doi:10.1111/j.1750-3841.2012.02756.x .

Godevac, Dejan, Tešević, Vele V., Vajs, Vlatka, Milosavljević, Slobodan, Zdunic, Gordana, Ordevic, Boban, Stanković, Miroslava, "Chemical Composition of Currant Seed Extracts and Their Protective Effect on Human Lymphocytes DNA" in Journal of Food Science, 77, no. 7 (2012):C779-C783,
https://doi.org/10.1111/j.1750-3841.2012.02756.x . .

TY  - JOUR
AU  - Godevac, Dejan
AU  - Tešević, Vele V.
AU  - Vajs, Vlatka
AU  - Milosavljević, Slobodan
AU  - Stanković, Miroslava
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4492
AB  - The tentative identification of seed extracts from 3 cultivars of blackberry (blackberry seed extracts [BSEs]) constituents was performed by LC/UV/MS technique. The identified compounds belonged to ellagitannins, galic acid derivatives, and ellagic acid derivatives. Two ellagitannins, Lambertianin C and Sanguiniin H-6, and an ellagic acid derivative, 4-alpha-L-arabinofuranosylellagic acid, were isolated using semipreparative High-performance liquid chromatography. The structure elucidations were based on high resolution-mass spectrometry and nuclear magnetic resonance studies. The BSEs and 3 isolated pure compounds were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (MN) assay. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. Among the tested extracts, the seeds of cv. Thornfree at concentration of 1 mu g/mL exhibit the most prominent effect decreasing the frequency of MN by 62.4%, when compared with the controls cell cultures. Antioxidant potential of pure ellagitannins cannot explain the strong effect of BSEs. The assumption was that better antioxidant effect of BSEs result from synergistic effects of individual compounds contained in the extracts and/or some minor components possessed strong activity.
T2  - Journal of Food Science
T1  - Blackberry Seed Extracts and Isolated Polyphenolic Compounds Showing Protective Effect on Human Lymphocytes DNA
VL  - 76
IS  - 7
SP  - C1039
EP  - C1043
DO  - 10.1111/j.1750-3841.2011.02305.x
ER  - 

@article{
author = "Godevac, Dejan and Tešević, Vele V. and Vajs, Vlatka and Milosavljević, Slobodan and Stanković, Miroslava",
year = "2011",
abstract = "The tentative identification of seed extracts from 3 cultivars of blackberry (blackberry seed extracts [BSEs]) constituents was performed by LC/UV/MS technique. The identified compounds belonged to ellagitannins, galic acid derivatives, and ellagic acid derivatives. Two ellagitannins, Lambertianin C and Sanguiniin H-6, and an ellagic acid derivative, 4-alpha-L-arabinofuranosylellagic acid, were isolated using semipreparative High-performance liquid chromatography. The structure elucidations were based on high resolution-mass spectrometry and nuclear magnetic resonance studies. The BSEs and 3 isolated pure compounds were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (MN) assay. The frequency of MN was scored in binucleated cells, and nuclear proliferation index was calculated. Among the tested extracts, the seeds of cv. Thornfree at concentration of 1 mu g/mL exhibit the most prominent effect decreasing the frequency of MN by 62.4%, when compared with the controls cell cultures. Antioxidant potential of pure ellagitannins cannot explain the strong effect of BSEs. The assumption was that better antioxidant effect of BSEs result from synergistic effects of individual compounds contained in the extracts and/or some minor components possessed strong activity.",
journal = "Journal of Food Science",
title = "Blackberry Seed Extracts and Isolated Polyphenolic Compounds Showing Protective Effect on Human Lymphocytes DNA",
volume = "76",
number = "7",
pages = "C1039-C1043",
doi = "10.1111/j.1750-3841.2011.02305.x"
}

Godevac, D., Tešević, V. V., Vajs, V., Milosavljević, S.,& Stanković, M.. (2011). Blackberry Seed Extracts and Isolated Polyphenolic Compounds Showing Protective Effect on Human Lymphocytes DNA. in Journal of Food Science, 76(7), C1039-C1043.
https://doi.org/10.1111/j.1750-3841.2011.02305.x

Godevac D, Tešević VV, Vajs V, Milosavljević S, Stanković M. Blackberry Seed Extracts and Isolated Polyphenolic Compounds Showing Protective Effect on Human Lymphocytes DNA. in Journal of Food Science. 2011;76(7):C1039-C1043.
doi:10.1111/j.1750-3841.2011.02305.x .

Godevac, Dejan, Tešević, Vele V., Vajs, Vlatka, Milosavljević, Slobodan, Stanković, Miroslava, "Blackberry Seed Extracts and Isolated Polyphenolic Compounds Showing Protective Effect on Human Lymphocytes DNA" in Journal of Food Science, 76, no. 7 (2011):C1039-C1043,
https://doi.org/10.1111/j.1750-3841.2011.02305.x . .

TY  - JOUR
AU  - Aljancic, Ivana
AU  - Stanković, Miroslava
AU  - Tešević, Vele V.
AU  - Vujisić, Ljubodrag
AU  - Vajs, Vlatka
AU  - Milosavljević, Slobodan
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4002
AB  - This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.
T2  - Natural Product Communications
T1  - Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species
VL  - 5
IS  - 5
SP  - 729
EP  - 732
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4002
ER  - 

@article{
author = "Aljancic, Ivana and Stanković, Miroslava and Tešević, Vele V. and Vujisić, Ljubodrag and Vajs, Vlatka and Milosavljević, Slobodan",
year = "2010",
abstract = "This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.",
journal = "Natural Product Communications",
title = "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species",
volume = "5",
number = "5",
pages = "729-732",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4002"
}

Aljancic, I., Stanković, M., Tešević, V. V., Vujisić, L., Vajs, V.,& Milosavljević, S.. (2010). Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications, 5(5), 729-732.
https://hdl.handle.net/21.15107/rcub_vinar_4002

Aljancic I, Stanković M, Tešević VV, Vujisić L, Vajs V, Milosavljević S. Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications. 2010;5(5):729-732.
https://hdl.handle.net/21.15107/rcub_vinar_4002 .

Aljancic, Ivana, Stanković, Miroslava, Tešević, Vele V., Vujisić, Ljubodrag, Vajs, Vlatka, Milosavljević, Slobodan, "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species" in Natural Product Communications, 5, no. 5 (2010):729-732,
https://hdl.handle.net/21.15107/rcub_vinar_4002 .

TY  - JOUR
AU  - Vučković, Ivan
AU  - Vajs, Vlatka
AU  - Stanković, Miroslava
AU  - Tešević, Vele V.
AU  - Milosavljević, Slobodan
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3964
AB  - A new prenylated flavanonol named seselinonol (1) was isolated from the roots of Seseli annuum, together with the well-known biologically active polyacetylenes falcarinol (2) and falcarindiol (3), and the prenylated furanocoumarin phellopterin (4). Its structure was elucidated by extensive spectroscopic analysis, including HR-ESI-MS, ID- and 2D-NMR. Seselinonol and phellopterin were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. The new compound exerted a beneficial effect by decreasing DNA damage of human lymphocytes.
T2  - Chemistry and Biodiversity
T1  - A New Prenylated Flavanonol from Seseli annuum Roots Showing Protective Effect on Human Lymphocytes DNA
VL  - 7
IS  - 3
SP  - 698
EP  - 704
DO  - 10.1002/cbdv.200900067
ER  - 

@article{
author = "Vučković, Ivan and Vajs, Vlatka and Stanković, Miroslava and Tešević, Vele V. and Milosavljević, Slobodan",
year = "2010",
abstract = "A new prenylated flavanonol named seselinonol (1) was isolated from the roots of Seseli annuum, together with the well-known biologically active polyacetylenes falcarinol (2) and falcarindiol (3), and the prenylated furanocoumarin phellopterin (4). Its structure was elucidated by extensive spectroscopic analysis, including HR-ESI-MS, ID- and 2D-NMR. Seselinonol and phellopterin were tested for in vitro protective effect on chromosome aberrations in peripheral human lymphocytes using cytochalasin-B blocked micronucleus (CBMN) assay. The new compound exerted a beneficial effect by decreasing DNA damage of human lymphocytes.",
journal = "Chemistry and Biodiversity",
title = "A New Prenylated Flavanonol from Seseli annuum Roots Showing Protective Effect on Human Lymphocytes DNA",
volume = "7",
number = "3",
pages = "698-704",
doi = "10.1002/cbdv.200900067"
}

Vučković, I., Vajs, V., Stanković, M., Tešević, V. V.,& Milosavljević, S.. (2010). A New Prenylated Flavanonol from Seseli annuum Roots Showing Protective Effect on Human Lymphocytes DNA. in Chemistry and Biodiversity, 7(3), 698-704.
https://doi.org/10.1002/cbdv.200900067

Vučković I, Vajs V, Stanković M, Tešević VV, Milosavljević S. A New Prenylated Flavanonol from Seseli annuum Roots Showing Protective Effect on Human Lymphocytes DNA. in Chemistry and Biodiversity. 2010;7(3):698-704.
doi:10.1002/cbdv.200900067 .

Vučković, Ivan, Vajs, Vlatka, Stanković, Miroslava, Tešević, Vele V., Milosavljević, Slobodan, "A New Prenylated Flavanonol from Seseli annuum Roots Showing Protective Effect on Human Lymphocytes DNA" in Chemistry and Biodiversity, 7, no. 3 (2010):698-704,
https://doi.org/10.1002/cbdv.200900067 . .

TY  - JOUR
AU  - Tešević, Vele V.
AU  - Nikicevic, Ninoslav
AU  - Milosavljević, Slobodan
AU  - Bajic, Danica
AU  - Vajs, Vlatka
AU  - Vučković, Ivan
AU  - Vujisić, Ljubodrag
AU  - Dordevic, Iris
AU  - Stanković, Miroslava
AU  - Velickovic, Milovan
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3651
AB  - In this study, volatile compounds were analyzed in five samples of home-made spirit beverage made by the distillation of fermented fruits of cornelian cherry (Cornus mas L.). The major volatile compounds, besides ethanol, identified and quantified were: methanol, acetaldehyde, I-propanol, ethyl acetate, 2-methyl-1-propanol, 1-butanol, amyl alcohols, 1-hexanol and 2-phenyl-ethanol, The minor volatiles were submitted to liquid liquid extraction with dichloromethane and analyzed by gas chromatography and gas chromatography/ /mass spectrometry (GC/MS). A total of 84 compounds were identified. The most abundant compounds were straight-chain free fatty acids, ethyl esters of C(6)-C(18) acids, limonene, 2-phenylethanol and 4-ethylphenol. Most of the compounds found in the Drenja spirits investigated in this study arc similar to those present in other alcoholic beverages.
T2  - Journal of the Serbian Chemical Society
T1  - Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry
VL  - 74
IS  - 2
SP  - 117
EP  - 128
DO  - 10.2298/JSC0902117T
ER  - 

@article{
author = "Tešević, Vele V. and Nikicevic, Ninoslav and Milosavljević, Slobodan and Bajic, Danica and Vajs, Vlatka and Vučković, Ivan and Vujisić, Ljubodrag and Dordevic, Iris and Stanković, Miroslava and Velickovic, Milovan",
year = "2009",
abstract = "In this study, volatile compounds were analyzed in five samples of home-made spirit beverage made by the distillation of fermented fruits of cornelian cherry (Cornus mas L.). The major volatile compounds, besides ethanol, identified and quantified were: methanol, acetaldehyde, I-propanol, ethyl acetate, 2-methyl-1-propanol, 1-butanol, amyl alcohols, 1-hexanol and 2-phenyl-ethanol, The minor volatiles were submitted to liquid liquid extraction with dichloromethane and analyzed by gas chromatography and gas chromatography/ /mass spectrometry (GC/MS). A total of 84 compounds were identified. The most abundant compounds were straight-chain free fatty acids, ethyl esters of C(6)-C(18) acids, limonene, 2-phenylethanol and 4-ethylphenol. Most of the compounds found in the Drenja spirits investigated in this study arc similar to those present in other alcoholic beverages.",
journal = "Journal of the Serbian Chemical Society",
title = "Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry",
volume = "74",
number = "2",
pages = "117-128",
doi = "10.2298/JSC0902117T"
}

Tešević, V. V., Nikicevic, N., Milosavljević, S., Bajic, D., Vajs, V., Vučković, I., Vujisić, L., Dordevic, I., Stanković, M.,& Velickovic, M.. (2009). Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry. in Journal of the Serbian Chemical Society, 74(2), 117-128.
https://doi.org/10.2298/JSC0902117T

Tešević VV, Nikicevic N, Milosavljević S, Bajic D, Vajs V, Vučković I, Vujisić L, Dordevic I, Stanković M, Velickovic M. Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry. in Journal of the Serbian Chemical Society. 2009;74(2):117-128.
doi:10.2298/JSC0902117T .

Tešević, Vele V., Nikicevic, Ninoslav, Milosavljević, Slobodan, Bajic, Danica, Vajs, Vlatka, Vučković, Ivan, Vujisić, Ljubodrag, Dordevic, Iris, Stanković, Miroslava, Velickovic, Milovan, "Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry" in Journal of the Serbian Chemical Society, 74, no. 2 (2009):117-128,
https://doi.org/10.2298/JSC0902117T . .

TY  - JOUR
AU  - Godevac, Dejan
AU  - Tešević, Vele V.
AU  - Vajs, Vlatka
AU  - Milosavljević, Slobodan
AU  - Stanković, Miroslava
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3828
AB  - This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential benefits of polyphenolic compounds from raspberry seeds as efficient antioxidants. (C) 2009 Elsevier Ltd. All rights reserved.
T2  - Food and Chemical Toxicology
T1  - Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes
VL  - 47
IS  - 11
SP  - 2853
EP  - 2859
DO  - 10.1016/j.fct.2009.09.006
ER  - 

@article{
author = "Godevac, Dejan and Tešević, Vele V. and Vajs, Vlatka and Milosavljević, Slobodan and Stanković, Miroslava",
year = "2009",
abstract = "This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential benefits of polyphenolic compounds from raspberry seeds as efficient antioxidants. (C) 2009 Elsevier Ltd. All rights reserved.",
journal = "Food and Chemical Toxicology",
title = "Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes",
volume = "47",
number = "11",
pages = "2853-2859",
doi = "10.1016/j.fct.2009.09.006"
}

Godevac, D., Tešević, V. V., Vajs, V., Milosavljević, S.,& Stanković, M.. (2009). Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes. in Food and Chemical Toxicology, 47(11), 2853-2859.
https://doi.org/10.1016/j.fct.2009.09.006

Godevac D, Tešević VV, Vajs V, Milosavljević S, Stanković M. Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes. in Food and Chemical Toxicology. 2009;47(11):2853-2859.
doi:10.1016/j.fct.2009.09.006 .

Godevac, Dejan, Tešević, Vele V., Vajs, Vlatka, Milosavljević, Slobodan, Stanković, Miroslava, "Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes" in Food and Chemical Toxicology, 47, no. 11 (2009):2853-2859,
https://doi.org/10.1016/j.fct.2009.09.006 . .

TY  - JOUR
AU  - Stanković, Miroslava
AU  - Horvat, Anica
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3505
AB  - This study addresses the in vitro protective effects of proanthocyanidins and quercetin on the frequency of micronuclei with reference to the antioxidant status of cultured human lymphocytes also subjected to 2 Gy of gamma-radiation. Treatment of lymphocytes with proanthocyanidins induced a significant decrease in the frequency of micronuclei and reduction of malonyldialdehyde production, as well as increased catalase and glutathione S-transferase activity Quercetin induces a higher incidence of micronuclei and production of malonyldialdehyde. The seven-fold increase of micronuclei frequency induced by a therapeutic dose of gamma-radiation was decreased in the presence of proanthocyanidins. These results demonstrate that proanthocyanidins may be important in the prevention of ROS-induced oxidative lymphocyte damage by decreasing DNA damage, lowering membrane lipid peroxidation, and increasing the activity AOP enzymes, as well as by reducing the level of gamma-radiation-induced DNA damage. Our results support the potential benefits of proanthocyanidins as efficient antioxidants and radioprotectors.
T2  - Archives of Biological Sciences
T1  - Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes
VL  - 60
IS  - 3
SP  - 367
EP  - 377
DO  - 10.2298/ABS0803367S
ER  - 

@article{
author = "Stanković, Miroslava and Horvat, Anica",
year = "2008",
abstract = "This study addresses the in vitro protective effects of proanthocyanidins and quercetin on the frequency of micronuclei with reference to the antioxidant status of cultured human lymphocytes also subjected to 2 Gy of gamma-radiation. Treatment of lymphocytes with proanthocyanidins induced a significant decrease in the frequency of micronuclei and reduction of malonyldialdehyde production, as well as increased catalase and glutathione S-transferase activity Quercetin induces a higher incidence of micronuclei and production of malonyldialdehyde. The seven-fold increase of micronuclei frequency induced by a therapeutic dose of gamma-radiation was decreased in the presence of proanthocyanidins. These results demonstrate that proanthocyanidins may be important in the prevention of ROS-induced oxidative lymphocyte damage by decreasing DNA damage, lowering membrane lipid peroxidation, and increasing the activity AOP enzymes, as well as by reducing the level of gamma-radiation-induced DNA damage. Our results support the potential benefits of proanthocyanidins as efficient antioxidants and radioprotectors.",
journal = "Archives of Biological Sciences",
title = "Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes",
volume = "60",
number = "3",
pages = "367-377",
doi = "10.2298/ABS0803367S"
}

Stanković, M.,& Horvat, A.. (2008). Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes. in Archives of Biological Sciences, 60(3), 367-377.
https://doi.org/10.2298/ABS0803367S

Stanković M, Horvat A. Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes. in Archives of Biological Sciences. 2008;60(3):367-377.
doi:10.2298/ABS0803367S .

Stanković, Miroslava, Horvat, Anica, "Properties of grape seed Proanthocyanidins and quercetin in human lymphocytes" in Archives of Biological Sciences, 60, no. 3 (2008):367-377,
https://doi.org/10.2298/ABS0803367S . .

TY  - JOUR
AU  - Stanković, Miroslava
AU  - Tešević, Vele V.
AU  - Vajs, Vlatka
AU  - Todorović, Nina
AU  - Milosavljević, Slobodan
AU  - Godevac, Dejan
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3481
AB  - The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and (13)C-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS.
T2  - Planta Medica
T1  - Antioxidant properties of grape seed extract on human lymphocyte oxidative defence
VL  - 74
IS  - 7
SP  - 730
EP  - 735
DO  - 10.1055/s-2008-1074521
ER  - 

@article{
author = "Stanković, Miroslava and Tešević, Vele V. and Vajs, Vlatka and Todorović, Nina and Milosavljević, Slobodan and Godevac, Dejan",
year = "2008",
abstract = "The distribution of polyphenolic compounds in a grape (Vitis vinifera) seed extract (GSE) was determined using LC/ESI-TOF MS, HPLC/DAD, and (13)C-NMR. The 17 identified compounds comprised gallic and protocatechuic acid, catetchin and epicatechin monomers, procyanidin oligomers, and procyanidin gallates. This study addresses the in vitro effects of grape seed extract (GSE) on the frequency of micronuclei with reference to the antioxidant status in human lymphocytes. To establish the most effective protective support, we used four different concentrations of GSE, in the range 1-6 mu g/mL. Treatment of lymphocytes with GSE at a concentration of 2.5 mu g/mL induced a significant decrease in the frequency of micronuclei by 40%, reduction of malonyldialdehyde production by 30%, while a concentration of 5 mu g/mL increased catalase and glutathione S-transferase activity by 10 % and 15 %, respectively. These results demonstrate that GSE may be effective in the prevention of oxidative lymphocyte damage by ROS.",
journal = "Planta Medica",
title = "Antioxidant properties of grape seed extract on human lymphocyte oxidative defence",
volume = "74",
number = "7",
pages = "730-735",
doi = "10.1055/s-2008-1074521"
}

Stanković, M., Tešević, V. V., Vajs, V., Todorović, N., Milosavljević, S.,& Godevac, D.. (2008). Antioxidant properties of grape seed extract on human lymphocyte oxidative defence. in Planta Medica, 74(7), 730-735.
https://doi.org/10.1055/s-2008-1074521

Stanković M, Tešević VV, Vajs V, Todorović N, Milosavljević S, Godevac D. Antioxidant properties of grape seed extract on human lymphocyte oxidative defence. in Planta Medica. 2008;74(7):730-735.
doi:10.1055/s-2008-1074521 .

Stanković, Miroslava, Tešević, Vele V., Vajs, Vlatka, Todorović, Nina, Milosavljević, Slobodan, Godevac, Dejan, "Antioxidant properties of grape seed extract on human lymphocyte oxidative defence" in Planta Medica, 74, no. 7 (2008):730-735,
https://doi.org/10.1055/s-2008-1074521 . .

TY  - CONF
AU  - Stanković, Miroslava
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11697
AB  - U ovom radu je praćena promena učestalosti mikronukleusa(MN) i aktivnost antioksidativnih enzima katalaze(CAT) i glutation S transferaze (GST), kao i produkcija malondialdehida(MDA) u neozračenim i ozračenim ćelijskim kulturama humanih limfocita tretiranih 0,87μM flavonoidima (procijanidolom, kvercetinom, luteolinom i kemferolom). Rezultati su pokazali da najbolji antioksidativni efekat na ćelijske kulture humanih limfocita pokazuje procijanidol, antioksidativni efekat luteolina i kemferola je slabiji, dok kvercetin pokazuje i prooksidativnu aktivnost.
AB  - Phenolic components (flavonoids), may be important in prevention of DNA damage. We have tested the hypothesis that the flavonoids are protective against the DNA-damaging by γ-radiation in vitro. The study confirms strong antioxidant activity and radioprotective properties of flavonoids.
PB  - Beograd : Institut za nuklearne nauke "Vinča" : Društvo za zaštitu od zračenja Srbije i Crne Gore
C3  - 24. simpozijum DZZSCG : zbornik radova
T1  - Antioksidantna aktivnost flavonoida (procijanidola, kvercetina, kemferola i luteolina) u humanim limfocitima ozračenim in vitro
T1  - The antioxidants activity of flavonoids (procyanidol, quercetin, luteolin and kaempherol) in human lymphocytes exposed to ionizing radiation in vitro
SP  - 201
EP  - 205
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11697
ER  - 

@conference{
author = "Stanković, Miroslava and Leskovac, Andreja and Petrović, Sandra",
year = "2007",
abstract = "U ovom radu je praćena promena učestalosti mikronukleusa(MN) i aktivnost antioksidativnih enzima katalaze(CAT) i glutation S transferaze (GST), kao i produkcija malondialdehida(MDA) u neozračenim i ozračenim ćelijskim kulturama humanih limfocita tretiranih 0,87μM flavonoidima (procijanidolom, kvercetinom, luteolinom i kemferolom). Rezultati su pokazali da najbolji antioksidativni efekat na ćelijske kulture humanih limfocita pokazuje procijanidol, antioksidativni efekat luteolina i kemferola je slabiji, dok kvercetin pokazuje i prooksidativnu aktivnost., Phenolic components (flavonoids), may be important in prevention of DNA damage. We have tested the hypothesis that the flavonoids are protective against the DNA-damaging by γ-radiation in vitro. The study confirms strong antioxidant activity and radioprotective properties of flavonoids.",
publisher = "Beograd : Institut za nuklearne nauke "Vinča" : Društvo za zaštitu od zračenja Srbije i Crne Gore",
journal = "24. simpozijum DZZSCG : zbornik radova",
title = "Antioksidantna aktivnost flavonoida (procijanidola, kvercetina, kemferola i luteolina) u humanim limfocitima ozračenim in vitro, The antioxidants activity of flavonoids (procyanidol, quercetin, luteolin and kaempherol) in human lymphocytes exposed to ionizing radiation in vitro",
pages = "201-205",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11697"
}

Stanković, M., Leskovac, A.,& Petrović, S.. (2007). Antioksidantna aktivnost flavonoida (procijanidola, kvercetina, kemferola i luteolina) u humanim limfocitima ozračenim in vitro. in 24. simpozijum DZZSCG : zbornik radova
Beograd : Institut za nuklearne nauke "Vinča" : Društvo za zaštitu od zračenja Srbije i Crne Gore., 201-205.
https://hdl.handle.net/21.15107/rcub_vinar_11697

Stanković M, Leskovac A, Petrović S. Antioksidantna aktivnost flavonoida (procijanidola, kvercetina, kemferola i luteolina) u humanim limfocitima ozračenim in vitro. in 24. simpozijum DZZSCG : zbornik radova. 2007;:201-205.
https://hdl.handle.net/21.15107/rcub_vinar_11697 .

Stanković, Miroslava, Leskovac, Andreja, Petrović, Sandra, "Antioksidantna aktivnost flavonoida (procijanidola, kvercetina, kemferola i luteolina) u humanim limfocitima ozračenim in vitro" in 24. simpozijum DZZSCG : zbornik radova (2007):201-205,
https://hdl.handle.net/21.15107/rcub_vinar_11697 .

TY  - JOUR
AU  - Milovanović, Vesna
AU  - Radulovic, Niko
AU  - Todorović, Zoran
AU  - Stanković, Miroslava
AU  - Stojanović, Gordana
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3279
AB  - The hydro-alcoholic extracts of five Equisetum species, E. arvense L., E. sylvaticum L., E. fluviatile L., E. palustre L. and E. telmateia Ehrh., growing-wild in Serbia were evaluated for their genotoxicity, antimicrobial activity, antioxidant capacity and the results related to the total phenol content and HPLC flavonoid profiles. The total phenol content was 92-349 mu mol expressed as equivalents of chlorogenic acid per g of dried plant material. Main identified compounds were kaempferol-, quercetin- glycosides and caffeic acid derivatives. E. telmateia extract showed the greatest antioxidant capacity. Almost all tested microorganisms demonstrated some degree of sensitivity to the examined extracts. All tested extracts at 62.5 mu g/ml showed higher incidence of micronucleus formation than in the control sample. The obtained data allowed mutual comparison of examined species and their assessment as possible sources of antioxidants, antimicrobials and/or genotoxic substances.
T2  - Plant Foods for Human Nutrition
T1  - Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species
VL  - 62
IS  - 3
SP  - 113
EP  - 119
DO  - 10.1007/s11130-007-0050-z
ER  - 

@article{
author = "Milovanović, Vesna and Radulovic, Niko and Todorović, Zoran and Stanković, Miroslava and Stojanović, Gordana",
year = "2007",
abstract = "The hydro-alcoholic extracts of five Equisetum species, E. arvense L., E. sylvaticum L., E. fluviatile L., E. palustre L. and E. telmateia Ehrh., growing-wild in Serbia were evaluated for their genotoxicity, antimicrobial activity, antioxidant capacity and the results related to the total phenol content and HPLC flavonoid profiles. The total phenol content was 92-349 mu mol expressed as equivalents of chlorogenic acid per g of dried plant material. Main identified compounds were kaempferol-, quercetin- glycosides and caffeic acid derivatives. E. telmateia extract showed the greatest antioxidant capacity. Almost all tested microorganisms demonstrated some degree of sensitivity to the examined extracts. All tested extracts at 62.5 mu g/ml showed higher incidence of micronucleus formation than in the control sample. The obtained data allowed mutual comparison of examined species and their assessment as possible sources of antioxidants, antimicrobials and/or genotoxic substances.",
journal = "Plant Foods for Human Nutrition",
title = "Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species",
volume = "62",
number = "3",
pages = "113-119",
doi = "10.1007/s11130-007-0050-z"
}

Milovanović, V., Radulovic, N., Todorović, Z., Stanković, M.,& Stojanović, G.. (2007). Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species. in Plant Foods for Human Nutrition, 62(3), 113-119.
https://doi.org/10.1007/s11130-007-0050-z

Milovanović V, Radulovic N, Todorović Z, Stanković M, Stojanović G. Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species. in Plant Foods for Human Nutrition. 2007;62(3):113-119.
doi:10.1007/s11130-007-0050-z .

Milovanović, Vesna, Radulovic, Niko, Todorović, Zoran, Stanković, Miroslava, Stojanović, Gordana, "Antioxidant, antimicrobial and genotoxicity screening of hydro-alcoholic extracts of five serbian Equisetum species" in Plant Foods for Human Nutrition, 62, no. 3 (2007):113-119,
https://doi.org/10.1007/s11130-007-0050-z . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Stanković, Miroslava
AU  - Radovanović, Sandra
AU  - Dragić, Milena
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11734
T2  - Archive of Oncology
T1  - The use of cytochalasin block (CB) micronucleus test to identify clastogenic and antiproliferative action of tiazofurin
VL  - 9
IS  - 1
SP  - 47
EP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11734
ER  - 

@article{
author = "Joksić, Gordana and Stanković, Miroslava and Radovanović, Sandra and Dragić, Milena",
year = "2001",
journal = "Archive of Oncology",
title = "The use of cytochalasin block (CB) micronucleus test to identify clastogenic and antiproliferative action of tiazofurin",
volume = "9",
number = "1",
pages = "47-48",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11734"
}

Joksić, G., Stanković, M., Radovanović, S.,& Dragić, M.. (2001). The use of cytochalasin block (CB) micronucleus test to identify clastogenic and antiproliferative action of tiazofurin. in Archive of Oncology, 9(1), 47-48.
https://hdl.handle.net/21.15107/rcub_vinar_11734

Joksić G, Stanković M, Radovanović S, Dragić M. The use of cytochalasin block (CB) micronucleus test to identify clastogenic and antiproliferative action of tiazofurin. in Archive of Oncology. 2001;9(1):47-48.
https://hdl.handle.net/21.15107/rcub_vinar_11734 .

Joksić, Gordana, Stanković, Miroslava, Radovanović, Sandra, Dragić, Milena, "The use of cytochalasin block (CB) micronucleus test to identify clastogenic and antiproliferative action of tiazofurin" in Archive of Oncology, 9, no. 1 (2001):47-48,
https://hdl.handle.net/21.15107/rcub_vinar_11734 .