Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes
Апстракт
This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential bene...fits of polyphenolic compounds from raspberry seeds as efficient antioxidants. (C) 2009 Elsevier Ltd. All rights reserved.
Кључне речи:
Rubus idaeus / CBMN assay / LC/UV/ESI-TOF MS / Flavanol / EllagitanninИзвор:
Food and Chemical Toxicology, 2009, 47, 11, 2853-2859Финансирање / пројекти:
- Serbian Ministry of Science and Environmental Protection [142053]
DOI: 10.1016/j.fct.2009.09.006
ISSN: 0278-6915
PubMed: 19748543
WoS: 000272013400026
Scopus: 2-s2.0-70350344384
Колекције
Институција/група
VinčaTY - JOUR AU - Godevac, Dejan AU - Tešević, Vele V. AU - Vajs, Vlatka AU - Milosavljević, Slobodan AU - Stanković, Miroslava PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3828 AB - This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential benefits of polyphenolic compounds from raspberry seeds as efficient antioxidants. (C) 2009 Elsevier Ltd. All rights reserved. T2 - Food and Chemical Toxicology T1 - Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes VL - 47 IS - 11 SP - 2853 EP - 2859 DO - 10.1016/j.fct.2009.09.006 ER -
@article{ author = "Godevac, Dejan and Tešević, Vele V. and Vajs, Vlatka and Milosavljević, Slobodan and Stanković, Miroslava", year = "2009", abstract = "This study addresses in vitro effects of raspberry (Rubus idaeus) seed extracts (RSE) on the frequency of micronuclei. We evaluated the effects of three different extracts (50%, 80%, and 100% methanol) in doses of 1.4, 4.2, and 8.4 mu g/mL, per 5 mL culture using cytochalasin-B micronucleus (CBMN) assay in peripheral human lymphocytes. The frequency of MN was scored in binucleated (BN) cells. The nuclear proliferation index was also calculated. The distribution of polyphenolic compounds in RSEs was determined using LC/UV/ESI-TOF MS. The identified 37 compounds comprised flavanol monomers and oligomers, as well as varieties of ellagitannin components. Treatment of lymphocytes with RSEs induced a significant decrease in the frequency of micronuclei by 80%. These results demonstrate that the constituents of RSEs may be important in the prevention of oxidative lymphocyte damage by reactive oxygen species and may also reduce the level of DNA damage. These findings support the potential benefits of polyphenolic compounds from raspberry seeds as efficient antioxidants. (C) 2009 Elsevier Ltd. All rights reserved.", journal = "Food and Chemical Toxicology", title = "Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes", volume = "47", number = "11", pages = "2853-2859", doi = "10.1016/j.fct.2009.09.006" }
Godevac, D., Tešević, V. V., Vajs, V., Milosavljević, S.,& Stanković, M.. (2009). Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes. in Food and Chemical Toxicology, 47(11), 2853-2859. https://doi.org/10.1016/j.fct.2009.09.006
Godevac D, Tešević VV, Vajs V, Milosavljević S, Stanković M. Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes. in Food and Chemical Toxicology. 2009;47(11):2853-2859. doi:10.1016/j.fct.2009.09.006 .
Godevac, Dejan, Tešević, Vele V., Vajs, Vlatka, Milosavljević, Slobodan, Stanković, Miroslava, "Antioxidant properties of raspberry seed extracts on micronucleus distribution in peripheral blood lymphocytes" in Food and Chemical Toxicology, 47, no. 11 (2009):2853-2859, https://doi.org/10.1016/j.fct.2009.09.006 . .