TY  - JOUR
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Keta, Otilija D.
AU  - Privitera G.
AU  - Cirrone, G. A. P.
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10506
AB  - Deregulation of apoptosis commonly occurs in melanoma cells and could be a reason for resistance. The effectiveness of different treatments depends on their ability to activate this process. In this study the effects of combined treatments with fotemustine (FM) and proton irradiation on the regulators of apoptosis were analyzed. Sub-confluent HTB140 human melanoma cells were treated with FM (100, 250 µM) 24 h prior to irradiation (12, 16 Gy). Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak. Flow cytometric analysis of apoptosis and the Western blot analysis of apoptotic regulators were performed 6 or 48 h after treatments. Percent of apoptotic nuclei increased after applied treatments, reaching the level of 4 to 41 %. Induction of apoptosis was associated with p53 and Bax up regulation and Bcl-2 down regulation. The obtained results imply that analyzed treatments induce apoptosis through the activation of the mitochondrial apoptotic pathway, with better pro-apoptotic effects achieved by combined treatments.
T2  - INFN-LNS Activity report 2009
T1  - Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation
SP  - 234
EP  - 237
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10506
ER  - 

@article{
author = "Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Privitera G. and Cirrone, G. A. P. and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Deregulation of apoptosis commonly occurs in melanoma cells and could be a reason for resistance. The effectiveness of different treatments depends on their ability to activate this process. In this study the effects of combined treatments with fotemustine (FM) and proton irradiation on the regulators of apoptosis were analyzed. Sub-confluent HTB140 human melanoma cells were treated with FM (100, 250 µM) 24 h prior to irradiation (12, 16 Gy). Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak. Flow cytometric analysis of apoptosis and the Western blot analysis of apoptotic regulators were performed 6 or 48 h after treatments. Percent of apoptotic nuclei increased after applied treatments, reaching the level of 4 to 41 %. Induction of apoptosis was associated with p53 and Bax up regulation and Bcl-2 down regulation. The obtained results imply that analyzed treatments induce apoptosis through the activation of the mitochondrial apoptotic pathway, with better pro-apoptotic effects achieved by combined treatments.",
journal = "INFN-LNS Activity report 2009",
title = "Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation",
pages = "234-237",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10506"
}

Korićanac, L., Žakula, J., Keta, O. D., Privitera G., Cirrone, G. A. P., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2010). Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation. in INFN-LNS Activity report 2009, 234-237.
https://hdl.handle.net/21.15107/rcub_vinar_10506

Korićanac L, Žakula J, Keta OD, Privitera G., Cirrone GAP, Cuttone G, Petrović IM, Ristić-Fira A. Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation. in INFN-LNS Activity report 2009. 2010;:234-237.
https://hdl.handle.net/21.15107/rcub_vinar_10506 .

Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Privitera G., Cirrone, G. A. P., Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation" in INFN-LNS Activity report 2009 (2010):234-237,
https://hdl.handle.net/21.15107/rcub_vinar_10506 .

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Romano, F.
AU  - Cirrone, G. A. P.
AU  - Cuttone, Giacomo
AU  - Petrović, Ivan M.
AU  - Ristić-Fira, Aleksandra
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10625
AB  - Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation
T2  - INFN-LNS Activity report 2009
T1  - Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let
SP  - 238
EP  - 241
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10625
ER  - 

@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Romano, F. and Cirrone, G. A. P. and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra",
year = "2010",
abstract = "Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation",
journal = "INFN-LNS Activity report 2009",
title = "Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let",
pages = "238-241",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10625"
}

Žakula, J., Korićanac, L., Keta, O. D., Romano, F., Cirrone, G. A. P., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2010). Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let. in INFN-LNS Activity report 2009, 238-241.
https://hdl.handle.net/21.15107/rcub_vinar_10625

Žakula J, Korićanac L, Keta OD, Romano F, Cirrone GAP, Cuttone G, Petrović IM, Ristić-Fira A. Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let. in INFN-LNS Activity report 2009. 2010;:238-241.
https://hdl.handle.net/21.15107/rcub_vinar_10625 .

Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Romano, F., Cirrone, G. A. P., Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Apoptosis of HTBL40 melanoma cells induced by carbon ions of different let" in INFN-LNS Activity report 2009 (2010):238-241,
https://hdl.handle.net/21.15107/rcub_vinar_10625 .

TY  - JOUR
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Keta, Otilija D.
AU  - Romano, F.
AU  - Cirrone, G. A. P.
AU  - Cuttone, Giacomo
AU  - Ristić-Fira, Aleksandra
AU  - Petrović, Ivan M.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11379
AB  - Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation.
T2  - INFN-LNS Activity report 2009
T1  - Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET
SP  - 238
EP  - 241
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11379
ER  - 

@article{
author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Romano, F. and Cirrone, G. A. P. and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.",
year = "2010",
abstract = "Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation.",
journal = "INFN-LNS Activity report 2009",
title = "Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET",
pages = "238-241",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11379"
}

Žakula, J., Korićanac, L., Keta, O. D., Romano, F., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2010). Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET. in INFN-LNS Activity report 2009, 238-241.
https://hdl.handle.net/21.15107/rcub_vinar_11379

Žakula J, Korićanac L, Keta OD, Romano F, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET. in INFN-LNS Activity report 2009. 2010;:238-241.
https://hdl.handle.net/21.15107/rcub_vinar_11379 .

Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Romano, F., Cirrone, G. A. P., Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET" in INFN-LNS Activity report 2009 (2010):238-241,
https://hdl.handle.net/21.15107/rcub_vinar_11379 .