Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation
Само за регистроване кориснике
2010
Аутори
Korićanac, LelaŽakula, Jelena
Keta, Otilija D.
Privitera G.
Cirrone, G. A. P.
Cuttone, Giacomo
Petrović, Ivan M.
Ristić-Fira, Aleksandra
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Deregulation of apoptosis commonly occurs in melanoma cells and could be a reason for resistance. The effectiveness of different treatments depends on their ability to activate this process. In this study the effects of combined treatments with fotemustine (FM) and proton irradiation on the regulators of apoptosis were analyzed. Sub-confluent HTB140 human melanoma cells were treated with FM (100, 250 µM) 24 h prior to irradiation (12, 16 Gy). Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak. Flow cytometric analysis of apoptosis and the Western blot analysis of apoptotic regulators were performed 6 or 48 h after treatments. Percent of apoptotic nuclei increased after applied treatments, reaching the level of 4 to 41 %. Induction of apoptosis was associated with p53 and Bax up regulation and Bcl-2 down regulation. The obtained results imply that analyzed treatments induce apoptosis through the activation of the mitochondrial apoptotic pathway, w...ith better pro-apoptotic effects achieved by combined treatments.
Извор:
INFN-LNS Activity report 2009, 2010, 234-237Финансирање / пројекти:
- Сигнални путеви деловања стероидних хормона и утицај ендогених и егзогених фактора на модулацију процеса у ћелијама сисара (RS-MESTD-MPN2006-2010-143044)
- Истраживања екотоксиколошких аспеката деловања ксенобиотика и биотичких агенаса на популације мишоликих глодара (RS-MESTD-MPN2006-2010-143038)
- INFN-LNS, Italy
Институција/група
VinčaTY - JOUR AU - Korićanac, Lela AU - Žakula, Jelena AU - Keta, Otilija D. AU - Privitera G. AU - Cirrone, G. A. P. AU - Cuttone, Giacomo AU - Petrović, Ivan M. AU - Ristić-Fira, Aleksandra PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10506 AB - Deregulation of apoptosis commonly occurs in melanoma cells and could be a reason for resistance. The effectiveness of different treatments depends on their ability to activate this process. In this study the effects of combined treatments with fotemustine (FM) and proton irradiation on the regulators of apoptosis were analyzed. Sub-confluent HTB140 human melanoma cells were treated with FM (100, 250 µM) 24 h prior to irradiation (12, 16 Gy). Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak. Flow cytometric analysis of apoptosis and the Western blot analysis of apoptotic regulators were performed 6 or 48 h after treatments. Percent of apoptotic nuclei increased after applied treatments, reaching the level of 4 to 41 %. Induction of apoptosis was associated with p53 and Bax up regulation and Bcl-2 down regulation. The obtained results imply that analyzed treatments induce apoptosis through the activation of the mitochondrial apoptotic pathway, with better pro-apoptotic effects achieved by combined treatments. T2 - INFN-LNS Activity report 2009 T1 - Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation SP - 234 EP - 237 UR - https://hdl.handle.net/21.15107/rcub_vinar_10506 ER -
@article{ author = "Korićanac, Lela and Žakula, Jelena and Keta, Otilija D. and Privitera G. and Cirrone, G. A. P. and Cuttone, Giacomo and Petrović, Ivan M. and Ristić-Fira, Aleksandra", year = "2010", abstract = "Deregulation of apoptosis commonly occurs in melanoma cells and could be a reason for resistance. The effectiveness of different treatments depends on their ability to activate this process. In this study the effects of combined treatments with fotemustine (FM) and proton irradiation on the regulators of apoptosis were analyzed. Sub-confluent HTB140 human melanoma cells were treated with FM (100, 250 µM) 24 h prior to irradiation (12, 16 Gy). Cells were irradiated in the middle of the therapeutic 62 MeV proton spread out Bragg peak. Flow cytometric analysis of apoptosis and the Western blot analysis of apoptotic regulators were performed 6 or 48 h after treatments. Percent of apoptotic nuclei increased after applied treatments, reaching the level of 4 to 41 %. Induction of apoptosis was associated with p53 and Bax up regulation and Bcl-2 down regulation. The obtained results imply that analyzed treatments induce apoptosis through the activation of the mitochondrial apoptotic pathway, with better pro-apoptotic effects achieved by combined treatments.", journal = "INFN-LNS Activity report 2009", title = "Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation", pages = "234-237", url = "https://hdl.handle.net/21.15107/rcub_vinar_10506" }
Korićanac, L., Žakula, J., Keta, O. D., Privitera G., Cirrone, G. A. P., Cuttone, G., Petrović, I. M.,& Ristić-Fira, A.. (2010). Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation. in INFN-LNS Activity report 2009, 234-237. https://hdl.handle.net/21.15107/rcub_vinar_10506
Korićanac L, Žakula J, Keta OD, Privitera G., Cirrone GAP, Cuttone G, Petrović IM, Ristić-Fira A. Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation. in INFN-LNS Activity report 2009. 2010;:234-237. https://hdl.handle.net/21.15107/rcub_vinar_10506 .
Korićanac, Lela, Žakula, Jelena, Keta, Otilija D., Privitera G., Cirrone, G. A. P., Cuttone, Giacomo, Petrović, Ivan M., Ristić-Fira, Aleksandra, "Alteration of p53 and Bax/ Bcl-2 ratio by fotemustine and proton irradiation" in INFN-LNS Activity report 2009 (2010):234-237, https://hdl.handle.net/21.15107/rcub_vinar_10506 .