Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET
Само за регистроване кориснике
2010
Аутори
Žakula, JelenaKorićanac, Lela
Keta, Otilija D.
Romano, F.
Cirrone, G. A. P.
Cuttone, Giacomo
Ristić-Fira, Aleksandra
Petrović, Ivan M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regula...tion.
Извор:
INFN-LNS Activity report 2009, 2010, 238-241Финансирање / пројекти:
- Сигнални путеви деловања стероидних хормона и утицај ендогених и егзогених фактора на модулацију процеса у ћелијама сисара (RS-MESTD-MPN2006-2010-143044)
- Истраживања екотоксиколошких аспеката деловања ксенобиотика и биотичких агенаса на популације мишоликих глодара (RS-MESTD-MPN2006-2010-143038)
- INFN-LNS, Italy
Колекције
Институција/група
VinčaTY - JOUR AU - Žakula, Jelena AU - Korićanac, Lela AU - Keta, Otilija D. AU - Romano, F. AU - Cirrone, G. A. P. AU - Cuttone, Giacomo AU - Ristić-Fira, Aleksandra AU - Petrović, Ivan M. PY - 2010 UR - https://vinar.vin.bg.ac.rs/handle/123456789/11379 AB - Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation. T2 - INFN-LNS Activity report 2009 T1 - Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET SP - 238 EP - 241 UR - https://hdl.handle.net/21.15107/rcub_vinar_11379 ER -
@article{ author = "Žakula, Jelena and Korićanac, Lela and Keta, Otilija D. and Romano, F. and Cirrone, G. A. P. and Cuttone, Giacomo and Ristić-Fira, Aleksandra and Petrović, Ivan M.", year = "2010", abstract = "Exposure to irradiation can trigger the p53 tumor suppressor to induce cell growth arrest or apoptosis. This study was conducted in order to evaluate the ability of carbon ions to induce apoptosis. The HTB140 melanoma cells were irradiated at three positions along the Bragg curve of the 62 MeV/u 12C ion beams. In this way the cells were exposed to different high linear energy transfer (LET) values. The percentage of apoptotic cells was evaluated by flow-cytometry and the corresponding apoptotic indexes were calculated. The expression of apoptosis-associated proteins, p53 and Bax was estimated by Western blot analyses. A dose dependent increase of apoptosis was noticed in all irradiation positions. When moving along the Bragg curve, i.e., with the raise of LET, the level of apoptosis increased, but was somewhat attenuated for the highest LET value. The corresponding apoptotic indexes ranged from 2.45 to 7.71. Moreover, the induction of apoptosis was associated with p53 and Bax up regulation.", journal = "INFN-LNS Activity report 2009", title = "Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET", pages = "238-241", url = "https://hdl.handle.net/21.15107/rcub_vinar_11379" }
Žakula, J., Korićanac, L., Keta, O. D., Romano, F., Cirrone, G. A. P., Cuttone, G., Ristić-Fira, A.,& Petrović, I. M.. (2010). Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET. in INFN-LNS Activity report 2009, 238-241. https://hdl.handle.net/21.15107/rcub_vinar_11379
Žakula J, Korićanac L, Keta OD, Romano F, Cirrone GAP, Cuttone G, Ristić-Fira A, Petrović IM. Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET. in INFN-LNS Activity report 2009. 2010;:238-241. https://hdl.handle.net/21.15107/rcub_vinar_11379 .
Žakula, Jelena, Korićanac, Lela, Keta, Otilija D., Romano, F., Cirrone, G. A. P., Cuttone, Giacomo, Ristić-Fira, Aleksandra, Petrović, Ivan M., "Apoptosis of HTB140 melanoma cells induced by carbon ions of different LET" in INFN-LNS Activity report 2009 (2010):238-241, https://hdl.handle.net/21.15107/rcub_vinar_11379 .