TY  - JOUR
AU  - Valenta Šobot, Ana
AU  - Janić, Marijana
AU  - Popović, Iva
AU  - Lazarević-Pašti, Tamara
AU  - Momić, Tatjana
AU  - Krstić, Aleksandar D.
AU  - Filipović Tričković, Jelena
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13334
AB  - Traditional medicine has used sage (Salvia officinalis L.) preparations for centuries to prevent and treat various inflammatory and oxidative stress-induced conditions. The aim of this in vitro study was to determine the bioactive properties of a sage leave extract obtained with environmentally friendly aqueous extraction and lyophilisation in primary human peripheral blood cells. To that end we measured the total phenolic and flavonoid content (TPC and TFC, respectively) with gas chromatography-mass spectrometry (GC-MS). Non-cytotoxic concentrations determined with the trypan blue assay were used to assess the antioxidant (DPPH, ABTS, and PAB assay), antigenotoxic (CBMN assay), immunomodulatory (IL-1β and TNF-α), and neuroprotective effects (AChE inhibition). The extract contained high TPC (162 mg GAE/g of dry extract) and TFC (39.47 mg QE/g of dry extract) concentrations, while β-thujone content was unexpectedly low (below 0.9 %). Strong radical-scavenging activity combined with glutathione reductase activation led to a decrease in basal and H2 O 2 - induced oxidative stress and DNA damage. A decrease in TNF-α and increase in IL-1β levels suggest complex immunomodulatory response that could contribute to antioxidant and, together with mild AChE inhibition, neuroprotective effects. Overall, this study has demonstrated that aqueous sage leave extract reduces the levels of thujone, 1,8-cineole, pinene, and terpene ketones that could be toxic in high concentrations, while maintaining high concentrations of biologically active protective compounds which have a potential to prevent and/ or treat inflammatory and oxidative stress-related conditions.
AB  - Salvia officinalis L. stoljećima se koristi u tradicionalnoj medicini za prevenciju i liječenje raznih upalnih i oksidacijskim stresom izazvanih poremećaja. U ovoj studiji željeli smo ekstrahirati kaduljino lišće korištenjem ekološki prihvatljivog, “zelenog” pristupa vodenom ekstrakcijom i liofilizacijom te odrediti njegova bioaktivna svojstva u primarnim ljudskim perifernim krvnim stanicama. Ukupni sadržaj fenola i flavonoida i GC-MS korišteni su za karakterizaciju ekstrakta. Necitotoksične koncentracije, određene tripan plavim testom, analizirane su za procjenu antioksidacijskih (DPPH, ABTS i PAB test), antigenotoksičnih (CBMN test), imunomodulacijskih (IL-1β i TNF-α) i neuroprotektivnih učinaka (AChE inhibicija). Ekstrakt je sadržavao visoku koncentraciju ukupnih fenola (162 mg GAE/g liofilizata) i flavonoida (39,47 mg QE/g liofilizata), dok je sadržaj β-tujona bio neočekivano nizak (niži od 0,9 %). Snažna aktivnost hvatanja radikala u kombinaciji s aktivacijom glutation reduktaze dovela je do smanjenja bazalnog i H2 O 2 induciranog oksidacijskog stresa i oštećenja DNA. Smanjenje TNF-α i povišenje razine IL-1β sugeriraju kompleksan imunomodulatorni odgovor koji bi mogao pridonijeti antioksidacijskim i, zajedno s blagom inhibicijom AChE, neuroprotektivnim učincima. Sveukupno, ova je studija pokazala da vodena ekstrakcija kaduljina lišća smanjuje toksične spojeve kao što su tujon, 1,8-cineol, pinen i terpenski ketoni, a održava visoku koncentraciju biološki aktivnih zaštitnih spojeva u ekstraktu, što bi moglo imati potencijal za prevenciju i/ili liječenje oksidacijskih i upalnih poremećaja.
T2  - Arhiv za higijenu rada i toksikologiju
T1  - Aqueous sage leave extract attenuates inflammation and oxidant-induced genotoxicity in human peripheral blood mononuclear cells
T1  - Vodeni ekstrakt kaduljina lišća umanjuje upalu i oksidacijsku genotoksičnost u ljudskim mononuklearnim stanicama periferne krvi
VL  - 75
IS  - InPress
SP  - 105
EP  - 114
DO  - 10.2478/aiht-2024-75-3836
ER  - 

@article{
author = "Valenta Šobot, Ana and Janić, Marijana and Popović, Iva and Lazarević-Pašti, Tamara and Momić, Tatjana and Krstić, Aleksandar D. and Filipović Tričković, Jelena",
year = "2024",
abstract = "Traditional medicine has used sage (Salvia officinalis L.) preparations for centuries to prevent and treat various inflammatory and oxidative stress-induced conditions. The aim of this in vitro study was to determine the bioactive properties of a sage leave extract obtained with environmentally friendly aqueous extraction and lyophilisation in primary human peripheral blood cells. To that end we measured the total phenolic and flavonoid content (TPC and TFC, respectively) with gas chromatography-mass spectrometry (GC-MS). Non-cytotoxic concentrations determined with the trypan blue assay were used to assess the antioxidant (DPPH, ABTS, and PAB assay), antigenotoxic (CBMN assay), immunomodulatory (IL-1β and TNF-α), and neuroprotective effects (AChE inhibition). The extract contained high TPC (162 mg GAE/g of dry extract) and TFC (39.47 mg QE/g of dry extract) concentrations, while β-thujone content was unexpectedly low (below 0.9 %). Strong radical-scavenging activity combined with glutathione reductase activation led to a decrease in basal and H2 O 2 - induced oxidative stress and DNA damage. A decrease in TNF-α and increase in IL-1β levels suggest complex immunomodulatory response that could contribute to antioxidant and, together with mild AChE inhibition, neuroprotective effects. Overall, this study has demonstrated that aqueous sage leave extract reduces the levels of thujone, 1,8-cineole, pinene, and terpene ketones that could be toxic in high concentrations, while maintaining high concentrations of biologically active protective compounds which have a potential to prevent and/ or treat inflammatory and oxidative stress-related conditions., Salvia officinalis L. stoljećima se koristi u tradicionalnoj medicini za prevenciju i liječenje raznih upalnih i oksidacijskim stresom izazvanih poremećaja. U ovoj studiji željeli smo ekstrahirati kaduljino lišće korištenjem ekološki prihvatljivog, “zelenog” pristupa vodenom ekstrakcijom i liofilizacijom te odrediti njegova bioaktivna svojstva u primarnim ljudskim perifernim krvnim stanicama. Ukupni sadržaj fenola i flavonoida i GC-MS korišteni su za karakterizaciju ekstrakta. Necitotoksične koncentracije, određene tripan plavim testom, analizirane su za procjenu antioksidacijskih (DPPH, ABTS i PAB test), antigenotoksičnih (CBMN test), imunomodulacijskih (IL-1β i TNF-α) i neuroprotektivnih učinaka (AChE inhibicija). Ekstrakt je sadržavao visoku koncentraciju ukupnih fenola (162 mg GAE/g liofilizata) i flavonoida (39,47 mg QE/g liofilizata), dok je sadržaj β-tujona bio neočekivano nizak (niži od 0,9 %). Snažna aktivnost hvatanja radikala u kombinaciji s aktivacijom glutation reduktaze dovela je do smanjenja bazalnog i H2 O 2 induciranog oksidacijskog stresa i oštećenja DNA. Smanjenje TNF-α i povišenje razine IL-1β sugeriraju kompleksan imunomodulatorni odgovor koji bi mogao pridonijeti antioksidacijskim i, zajedno s blagom inhibicijom AChE, neuroprotektivnim učincima. Sveukupno, ova je studija pokazala da vodena ekstrakcija kaduljina lišća smanjuje toksične spojeve kao što su tujon, 1,8-cineol, pinen i terpenski ketoni, a održava visoku koncentraciju biološki aktivnih zaštitnih spojeva u ekstraktu, što bi moglo imati potencijal za prevenciju i/ili liječenje oksidacijskih i upalnih poremećaja.",
journal = "Arhiv za higijenu rada i toksikologiju",
title = "Aqueous sage leave extract attenuates inflammation and oxidant-induced genotoxicity in human peripheral blood mononuclear cells, Vodeni ekstrakt kaduljina lišća umanjuje upalu i oksidacijsku genotoksičnost u ljudskim mononuklearnim stanicama periferne krvi",
volume = "75",
number = "InPress",
pages = "105-114",
doi = "10.2478/aiht-2024-75-3836"
}

Valenta Šobot, A., Janić, M., Popović, I., Lazarević-Pašti, T., Momić, T., Krstić, A. D.,& Filipović Tričković, J.. (2024). Aqueous sage leave extract attenuates inflammation and oxidant-induced genotoxicity in human peripheral blood mononuclear cells. in Arhiv za higijenu rada i toksikologiju, 75(InPress), 105-114.
https://doi.org/10.2478/aiht-2024-75-3836

Valenta Šobot A, Janić M, Popović I, Lazarević-Pašti T, Momić T, Krstić AD, Filipović Tričković J. Aqueous sage leave extract attenuates inflammation and oxidant-induced genotoxicity in human peripheral blood mononuclear cells. in Arhiv za higijenu rada i toksikologiju. 2024;75(InPress):105-114.
doi:10.2478/aiht-2024-75-3836 .

Valenta Šobot, Ana, Janić, Marijana, Popović, Iva, Lazarević-Pašti, Tamara, Momić, Tatjana, Krstić, Aleksandar D., Filipović Tričković, Jelena, "Aqueous sage leave extract attenuates inflammation and oxidant-induced genotoxicity in human peripheral blood mononuclear cells" in Arhiv za higijenu rada i toksikologiju, 75, no. InPress (2024):105-114,
https://doi.org/10.2478/aiht-2024-75-3836 . .

TY  - JOUR
AU  - Valenta Šobot, Ana
AU  - Drakulić, Dunja
AU  - Todorović, Ana
AU  - Janić, Marijana
AU  - Božović, Ana
AU  - Todorović, Lidija
AU  - Filipović Tričković, Jelena
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13330
AB  - Gentiopicroside (Gp) and swertiamarin (Sm), secoiridoid glycosides commonly found in plants of the Gentianaceae family, differ in one functional group. They exhibit promising cytotoxic effects in cancer cell lines and overall protective outcomes, marking them as promising molecules for developing novel pharmaceuticals. To investigate potential variations in cellular sensitivity to compounds of similar molecular structures, we analyzed the mode of Gp and Sm induced cell death in human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment. The lowest tested concentration that significantly reduces cell viability, 50 μ M, was applied. Oxidative stress parameters were estimated by measuring the levels of prooxidative/antioxidative balance, lipid peroxidation products, and 8-oxo-7,8-dihydro-2-deoxyguanosine, while gene expression of DNA repair enzymes was evaluated by employing quantitative real-time PCR. Cellular morphology was analyzed by fluorescent microscopy, and immunoblot analysis of apoptosis and necroptosis-related proteins was used to assess the type of cell death induced by the treatments. The discriminatory impact of Gp/Sm treatments on apoptosis and necroptosis- induced cell death was evaluated by monitoring the cell survival in co-treatment with specific cell death inhibitors. Obtained results show greater cytotoxicity of Gp than Sm suggesting that variations in the molecular structures of the tested compounds can substantially affect their biological effects. Gp/Sm co-treatment with apoptosis and necroptosis inhibitors revealed a distinct, albeit non-specific mechanism of PBMCs cell death. Although the therapeutic may not directly cause a specific type of cell death, its extent can be pivotal in assessing the safety of therapeutic application and developing phytopharmaceuticals with improved features. Since phytopharmaceuticals affect all exposed cells, identification of cytotoxic mechanisms on PBMCs after Gp and Sm treatment is important for addressing the formulation and dosage of potential phytopharmaceuticals.
T2  - Chemico-Biological Interactions
T1  - Gentiopicroside and swertiamarin induce non-selective oxidative stress-mediated cytotoxic effects in human peripheral blood mononuclear cells
VL  - 398
SP  - 111103
DO  - 10.1016/j.cbi.2024.111103
ER  - 

@article{
author = "Valenta Šobot, Ana and Drakulić, Dunja and Todorović, Ana and Janić, Marijana and Božović, Ana and Todorović, Lidija and Filipović Tričković, Jelena",
year = "2024",
abstract = "Gentiopicroside (Gp) and swertiamarin (Sm), secoiridoid glycosides commonly found in plants of the Gentianaceae family, differ in one functional group. They exhibit promising cytotoxic effects in cancer cell lines and overall protective outcomes, marking them as promising molecules for developing novel pharmaceuticals. To investigate potential variations in cellular sensitivity to compounds of similar molecular structures, we analyzed the mode of Gp and Sm induced cell death in human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment. The lowest tested concentration that significantly reduces cell viability, 50 μ M, was applied. Oxidative stress parameters were estimated by measuring the levels of prooxidative/antioxidative balance, lipid peroxidation products, and 8-oxo-7,8-dihydro-2-deoxyguanosine, while gene expression of DNA repair enzymes was evaluated by employing quantitative real-time PCR. Cellular morphology was analyzed by fluorescent microscopy, and immunoblot analysis of apoptosis and necroptosis-related proteins was used to assess the type of cell death induced by the treatments. The discriminatory impact of Gp/Sm treatments on apoptosis and necroptosis- induced cell death was evaluated by monitoring the cell survival in co-treatment with specific cell death inhibitors. Obtained results show greater cytotoxicity of Gp than Sm suggesting that variations in the molecular structures of the tested compounds can substantially affect their biological effects. Gp/Sm co-treatment with apoptosis and necroptosis inhibitors revealed a distinct, albeit non-specific mechanism of PBMCs cell death. Although the therapeutic may not directly cause a specific type of cell death, its extent can be pivotal in assessing the safety of therapeutic application and developing phytopharmaceuticals with improved features. Since phytopharmaceuticals affect all exposed cells, identification of cytotoxic mechanisms on PBMCs after Gp and Sm treatment is important for addressing the formulation and dosage of potential phytopharmaceuticals.",
journal = "Chemico-Biological Interactions",
title = "Gentiopicroside and swertiamarin induce non-selective oxidative stress-mediated cytotoxic effects in human peripheral blood mononuclear cells",
volume = "398",
pages = "111103",
doi = "10.1016/j.cbi.2024.111103"
}

Valenta Šobot, A., Drakulić, D., Todorović, A., Janić, M., Božović, A., Todorović, L.,& Filipović Tričković, J.. (2024). Gentiopicroside and swertiamarin induce non-selective oxidative stress-mediated cytotoxic effects in human peripheral blood mononuclear cells. in Chemico-Biological Interactions, 398, 111103.
https://doi.org/10.1016/j.cbi.2024.111103

Valenta Šobot A, Drakulić D, Todorović A, Janić M, Božović A, Todorović L, Filipović Tričković J. Gentiopicroside and swertiamarin induce non-selective oxidative stress-mediated cytotoxic effects in human peripheral blood mononuclear cells. in Chemico-Biological Interactions. 2024;398:111103.
doi:10.1016/j.cbi.2024.111103 .

Valenta Šobot, Ana, Drakulić, Dunja, Todorović, Ana, Janić, Marijana, Božović, Ana, Todorović, Lidija, Filipović Tričković, Jelena, "Gentiopicroside and swertiamarin induce non-selective oxidative stress-mediated cytotoxic effects in human peripheral blood mononuclear cells" in Chemico-Biological Interactions, 398 (2024):111103,
https://doi.org/10.1016/j.cbi.2024.111103 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Popović, Iva A.
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Jiménez, Maria Victoria
AU  - Algarra, Manuel
AU  - Dučić, Tanja
AU  - Stepić, Milutin
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13296
AB  - The literature data emphasize that nanoparticles might improve the beneficial effects of near-infrared light (NIR) on wound healing. This study investigates the mechanisms of the synergistic wound healing potential of NIR light and silver metal–organic frameworks combined with nitrogen- and sulfur-doped carbon dots (AgMOFsN-CDs and AgMOFsS-CDs, respectively), which was conducted by testing the fibroblasts viability, scratch assays, biochemical analysis, and synchrotron-based Fourier transform infrared (SR-FTIR) cell spectroscopy and imaging. Our findings reveal that the combined treatment of AgMOFsN-CDs and NIR light significantly increases cell viability to nearly 150% and promotes cell proliferation, with reduced interleukin-1 levels, suggesting an anti-inflammatory response. SR-FTIR spectroscopy shows this combined treatment results in unique protein alterations, including increased α-helix structures and reduced cross-β. Additionally, protein synthesis was enhanced upon the combined treatment. The likely mechanism behind the observed changes is the charge-specific interaction of N-CDs from the AgMOFsN-CDs with proteins, enhanced by NIR light due to the nanocomposite’s optical characteristics. Remarkably, the complete wound closure in the in vitro scratch assay was achieved exclusively with the combined NIR and AgMOFsN-CDs treatment, demonstrating the promising application of combined AgMOFsN-CDs with NIR light photodynamic therapy in regenerative nanomedicine and tissue engineering.
T2  - Pharmaceutics
T1  - Synergistic Enhancement of Targeted Wound Healing by Near-Infrared Photodynamic Therapy and Silver Metal–Organic Frameworks Combined with S- or N-Doped Carbon Dots
VL  - 16
IS  - 5
SP  - 671
DO  - 10.3390/pharmaceutics16050671
ER  - 

@article{
author = "Nešić, Maja D. and Popović, Iva A. and Žakula, Jelena and Korićanac, Lela and Filipović Tričković, Jelena and Valenta Šobot, Ana and Jiménez, Maria Victoria and Algarra, Manuel and Dučić, Tanja and Stepić, Milutin",
year = "2024",
abstract = "The literature data emphasize that nanoparticles might improve the beneficial effects of near-infrared light (NIR) on wound healing. This study investigates the mechanisms of the synergistic wound healing potential of NIR light and silver metal–organic frameworks combined with nitrogen- and sulfur-doped carbon dots (AgMOFsN-CDs and AgMOFsS-CDs, respectively), which was conducted by testing the fibroblasts viability, scratch assays, biochemical analysis, and synchrotron-based Fourier transform infrared (SR-FTIR) cell spectroscopy and imaging. Our findings reveal that the combined treatment of AgMOFsN-CDs and NIR light significantly increases cell viability to nearly 150% and promotes cell proliferation, with reduced interleukin-1 levels, suggesting an anti-inflammatory response. SR-FTIR spectroscopy shows this combined treatment results in unique protein alterations, including increased α-helix structures and reduced cross-β. Additionally, protein synthesis was enhanced upon the combined treatment. The likely mechanism behind the observed changes is the charge-specific interaction of N-CDs from the AgMOFsN-CDs with proteins, enhanced by NIR light due to the nanocomposite’s optical characteristics. Remarkably, the complete wound closure in the in vitro scratch assay was achieved exclusively with the combined NIR and AgMOFsN-CDs treatment, demonstrating the promising application of combined AgMOFsN-CDs with NIR light photodynamic therapy in regenerative nanomedicine and tissue engineering.",
journal = "Pharmaceutics",
title = "Synergistic Enhancement of Targeted Wound Healing by Near-Infrared Photodynamic Therapy and Silver Metal–Organic Frameworks Combined with S- or N-Doped Carbon Dots",
volume = "16",
number = "5",
pages = "671",
doi = "10.3390/pharmaceutics16050671"
}

Nešić, M. D., Popović, I. A., Žakula, J., Korićanac, L., Filipović Tričković, J., Valenta Šobot, A., Jiménez, M. V., Algarra, M., Dučić, T.,& Stepić, M.. (2024). Synergistic Enhancement of Targeted Wound Healing by Near-Infrared Photodynamic Therapy and Silver Metal–Organic Frameworks Combined with S- or N-Doped Carbon Dots. in Pharmaceutics, 16(5), 671.
https://doi.org/10.3390/pharmaceutics16050671

Nešić MD, Popović IA, Žakula J, Korićanac L, Filipović Tričković J, Valenta Šobot A, Jiménez MV, Algarra M, Dučić T, Stepić M. Synergistic Enhancement of Targeted Wound Healing by Near-Infrared Photodynamic Therapy and Silver Metal–Organic Frameworks Combined with S- or N-Doped Carbon Dots. in Pharmaceutics. 2024;16(5):671.
doi:10.3390/pharmaceutics16050671 .

Nešić, Maja D., Popović, Iva A., Žakula, Jelena, Korićanac, Lela, Filipović Tričković, Jelena, Valenta Šobot, Ana, Jiménez, Maria Victoria, Algarra, Manuel, Dučić, Tanja, Stepić, Milutin, "Synergistic Enhancement of Targeted Wound Healing by Near-Infrared Photodynamic Therapy and Silver Metal–Organic Frameworks Combined with S- or N-Doped Carbon Dots" in Pharmaceutics, 16, no. 5 (2024):671,
https://doi.org/10.3390/pharmaceutics16050671 . .

TY  - CONF
AU  - Butulija, Svetlana
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Todorović, Bratislav
AU  - Petrović, Sanja
AU  - Ilić, Bojana
AU  - Zmejkoski, Danica
AU  - Matović, Branko
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13167
PB  - Belgrade : Vinča Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade
PB  - Belgrade : Serbian Society for Innovative Materials in Extreme Conditions (SIM-EXTREME)
C3  - IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts
T1  - Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes
SP  - 73
EP  - 73
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13167
ER  - 

@conference{
author = "Butulija, Svetlana and Filipović Tričković, Jelena and Valenta Šobot, Ana and Todorović, Bratislav and Petrović, Sanja and Ilić, Bojana and Zmejkoski, Danica and Matović, Branko",
year = "2024",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade, Belgrade : Serbian Society for Innovative Materials in Extreme Conditions (SIM-EXTREME)",
journal = "IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts",
title = "Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes",
pages = "73-73",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13167"
}

Butulija, S., Filipović Tričković, J., Valenta Šobot, A., Todorović, B., Petrović, S., Ilić, B., Zmejkoski, D.,& Matović, B.. (2024). Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes. in IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts
Belgrade : Vinča Institute of Nuclear Sciences - National Institute of thе Republic of Serbia, University of Belgrade., 73-73.
https://hdl.handle.net/21.15107/rcub_vinar_13167

Butulija S, Filipović Tričković J, Valenta Šobot A, Todorović B, Petrović S, Ilić B, Zmejkoski D, Matović B. Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes. in IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts. 2024;:73-73.
https://hdl.handle.net/21.15107/rcub_vinar_13167 .

Butulija, Svetlana, Filipović Tričković, Jelena, Valenta Šobot, Ana, Todorović, Bratislav, Petrović, Sanja, Ilić, Bojana, Zmejkoski, Danica, Matović, Branko, "Bacterial Cellulose-Cerium Oxide Hydrogel for Tailored Redox Balance in Biomedical Extremes" in IMEC2024 - 2nd International Conference on Innovative Materials in Extreme Conditions : Book of abstracts (2024):73-73,
https://hdl.handle.net/21.15107/rcub_vinar_13167 .

TY  - CONF
AU  - Janić, Marijana
AU  - Valenta Šobot, Ana
AU  - Momić, Tatjana
AU  - Filipović Tričković, Jelena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13333
AB  - Sage (Salvia officinalis L.), a common member of the family Lamiaceae has been used as traditional herbal medicine against many diseases. It is reported that the plant has a variety of pharmacological effects, including anti- inflammatory, antibacterial, antiviral, hypoglycaemic, fungistatic, anticancer, antioxidative and antimutagenic effects. These effects are mainly due to the presence of phenolic compounds such as carnosic, rosmarinic, caffeic, salvianolic acids and flavonoids (luteolin-7-O-glucoside, quercetin and rutin). It is known that polyphenols are extracted with polar solvents, commonly with methanol, ethanol and their mixtures with water. Hence water is a polar solvent, it also can be considered as a potentially green solvent since it is non-toxic to health and the environment. In this study, we determined the total phenolic content (TPC) and total flavonoid content (TFC) in lyophilised extracts prepared with water extraction of sage leaves. The TPC of sage extracts (0.5 mg/mL, 0.25 mg/mL and 0.125 mg/mL) was determined using a colorimetric assay, with Folin–Ciocalteu reagent. TPC was expressed as mg of gallic acid equivalents (GAE) per g of dried extract (de). The TFC of sage extracts (1.75 mg/mL, 1.25 mg/mL and 0.75 mg/mL) was measured by the spectrophotometric method, based on the formation of a flavonoid-aluminium complex. TFC was expressed as mg of quercetin equivalents (QE) per g of dried extract (de). The results were presented as a mean value ± standard deviation (SD) of triplicate measurements. Our findings showed that TPC is in the range from 155 ± 5.55 to 166 ± 2.95 mg GAE/g de. TFC was in range from 31.7 ± 3.61 to 46.6 ± 2.54 mg QE/g de. Compared with published results where extractions of polyphenols from sage were carried out with methanol as an effective and toxic solvent, results obtained in our study demonstrated a high extraction yield of polyphenols from sage using water as a polar and non-toxic solvent.
PB  - Novi Sad : Faculty of Technical Sciences
C3  - DISC2023 : 3rd DIFENEW International Student Conference : Abstract book
T1  - Water as a Green Solvent for the Extraction of Polyphenols from Sage
SP  - 12
EP  - 12
ER  - 

@conference{
author = "Janić, Marijana and Valenta Šobot, Ana and Momić, Tatjana and Filipović Tričković, Jelena",
year = "2023",
abstract = "Sage (Salvia officinalis L.), a common member of the family Lamiaceae has been used as traditional herbal medicine against many diseases. It is reported that the plant has a variety of pharmacological effects, including anti- inflammatory, antibacterial, antiviral, hypoglycaemic, fungistatic, anticancer, antioxidative and antimutagenic effects. These effects are mainly due to the presence of phenolic compounds such as carnosic, rosmarinic, caffeic, salvianolic acids and flavonoids (luteolin-7-O-glucoside, quercetin and rutin). It is known that polyphenols are extracted with polar solvents, commonly with methanol, ethanol and their mixtures with water. Hence water is a polar solvent, it also can be considered as a potentially green solvent since it is non-toxic to health and the environment. In this study, we determined the total phenolic content (TPC) and total flavonoid content (TFC) in lyophilised extracts prepared with water extraction of sage leaves. The TPC of sage extracts (0.5 mg/mL, 0.25 mg/mL and 0.125 mg/mL) was determined using a colorimetric assay, with Folin–Ciocalteu reagent. TPC was expressed as mg of gallic acid equivalents (GAE) per g of dried extract (de). The TFC of sage extracts (1.75 mg/mL, 1.25 mg/mL and 0.75 mg/mL) was measured by the spectrophotometric method, based on the formation of a flavonoid-aluminium complex. TFC was expressed as mg of quercetin equivalents (QE) per g of dried extract (de). The results were presented as a mean value ± standard deviation (SD) of triplicate measurements. Our findings showed that TPC is in the range from 155 ± 5.55 to 166 ± 2.95 mg GAE/g de. TFC was in range from 31.7 ± 3.61 to 46.6 ± 2.54 mg QE/g de. Compared with published results where extractions of polyphenols from sage were carried out with methanol as an effective and toxic solvent, results obtained in our study demonstrated a high extraction yield of polyphenols from sage using water as a polar and non-toxic solvent.",
publisher = "Novi Sad : Faculty of Technical Sciences",
journal = "DISC2023 : 3rd DIFENEW International Student Conference : Abstract book",
title = "Water as a Green Solvent for the Extraction of Polyphenols from Sage",
pages = "12-12"
}

Janić, M., Valenta Šobot, A., Momić, T.,& Filipović Tričković, J.. (2023). Water as a Green Solvent for the Extraction of Polyphenols from Sage. in DISC2023 : 3rd DIFENEW International Student Conference : Abstract book
Novi Sad : Faculty of Technical Sciences., 12-12.

Janić M, Valenta Šobot A, Momić T, Filipović Tričković J. Water as a Green Solvent for the Extraction of Polyphenols from Sage. in DISC2023 : 3rd DIFENEW International Student Conference : Abstract book. 2023;:12-12..

Janić, Marijana, Valenta Šobot, Ana, Momić, Tatjana, Filipović Tričković, Jelena, "Water as a Green Solvent for the Extraction of Polyphenols from Sage" in DISC2023 : 3rd DIFENEW International Student Conference : Abstract book (2023):12-12.

TY  - CONF
AU  - Filipović Tričković, Jelena G.
AU  - Kokunešoski, Maja
AU  - Momić, Tatjana
AU  - Jovanović, Ivan G.
AU  - Anastasov, Marina
AU  - Stojanović, Dragana
AU  - Valenta Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11409
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book
T1  - Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures
T1  - Procena toksičnosti mezoporoznih nanočestica silicijum dioksida pri različitim postupcima ekstrakcije
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11409
ER  - 

@conference{
author = "Filipović Tričković, Jelena G. and Kokunešoski, Maja and Momić, Tatjana and Jovanović, Ivan G. and Anastasov, Marina and Stojanović, Dragana and Valenta Šobot, Ana",
year = "2023",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book",
title = "Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures, Procena toksičnosti mezoporoznih nanočestica silicijum dioksida pri različitim postupcima ekstrakcije",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11409"
}

Filipović Tričković, J. G., Kokunešoski, M., Momić, T., Jovanović, I. G., Anastasov, M., Stojanović, D.,& Valenta Šobot, A.. (2023). Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book.
https://hdl.handle.net/21.15107/rcub_vinar_11409

Filipović Tričković JG, Kokunešoski M, Momić T, Jovanović IG, Anastasov M, Stojanović D, Valenta Šobot A. Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_11409 .

Filipović Tričković, Jelena G., Kokunešoski, Maja, Momić, Tatjana, Jovanović, Ivan G., Anastasov, Marina, Stojanović, Dragana, Valenta Šobot, Ana, "Toxicity assessment of mesoporous silica nanoparticles under different extraction procedures" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book (2023),
https://hdl.handle.net/21.15107/rcub_vinar_11409 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Drakulić, Dunja
AU  - Kokunešoski, Maja
AU  - Momić, Tatjana
AU  - Filipović Tričković, Jelena G.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11410
C3  - 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book
T1  - Loganic acid induces apoptosis in human peripheral blood mononuclear cells
T1  - Loganska kiselina indukuje apoptozu u mononuklearnim ćelijama periferne krvi čoveka
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11410
ER  - 

@conference{
author = "Valenta Šobot, Ana and Drakulić, Dunja and Kokunešoski, Maja and Momić, Tatjana and Filipović Tričković, Jelena G.",
year = "2023",
journal = "13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book",
title = "Loganic acid induces apoptosis in human peripheral blood mononuclear cells, Loganska kiselina indukuje apoptozu u mononuklearnim ćelijama periferne krvi čoveka",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11410"
}

Valenta Šobot, A., Drakulić, D., Kokunešoski, M., Momić, T.,& Filipović Tričković, J. G.. (2023). Loganic acid induces apoptosis in human peripheral blood mononuclear cells. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book.
https://hdl.handle.net/21.15107/rcub_vinar_11410

Valenta Šobot A, Drakulić D, Kokunešoski M, Momić T, Filipović Tričković JG. Loganic acid induces apoptosis in human peripheral blood mononuclear cells. in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book. 2023;.
https://hdl.handle.net/21.15107/rcub_vinar_11410 .

Valenta Šobot, Ana, Drakulić, Dunja, Kokunešoski, Maja, Momić, Tatjana, Filipović Tričković, Jelena G., "Loganic acid induces apoptosis in human peripheral blood mononuclear cells" in 13th international congress of the Serbian society of toxicology & 1st toxSEE regional conference : abstract book (2023),
https://hdl.handle.net/21.15107/rcub_vinar_11410 .

TY  - CONF
AU  - Popović, I.
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Ralić, V.
AU  - Abu el Rub, Anamarija
AU  - Algarra, M.
AU  - Petković, M.
AU  - Stepić, Milutin
AU  - Nešić, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11808
AB  - In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
T1  - Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing
SP  - 78
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11808
ER  - 

@conference{
author = "Popović, I. and Valenta Šobot, Ana and Filipović Tričković, Jelena and Korićanac, Lela and Žakula, Jelena and Ralić, V. and Abu el Rub, Anamarija and Algarra, M. and Petković, M. and Stepić, Milutin and Nešić, M.",
year = "2023",
abstract = "In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade",
title = "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing",
pages = "78-78",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11808"
}

Popović, I., Valenta Šobot, A., Filipović Tričković, J., Korićanac, L., Žakula, J., Ralić, V., Abu el Rub, A., Algarra, M., Petković, M., Stepić, M.,& Nešić, M.. (2023). Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808

Popović I, Valenta Šobot A, Filipović Tričković J, Korićanac L, Žakula J, Ralić V, Abu el Rub A, Algarra M, Petković M, Stepić M, Nešić M. Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade. 2023;:78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

Popović, I., Valenta Šobot, Ana, Filipović Tričković, Jelena, Korićanac, Lela, Žakula, Jelena, Ralić, V., Abu el Rub, Anamarija, Algarra, M., Petković, M., Stepić, Milutin, Nešić, M., "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing" in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade (2023):78-78,
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

TY  - CONF
AU  - Nešić, M. D.
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Popović, I.
AU  - Soto, J.
AU  - Algarra, M.
AU  - Ralić, V.
AU  - Abu el Rub, Anamarija
AU  - Matijević, M.
AU  - Stepić, Milutin
AU  - Petković, M.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11809
AB  - Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
T1  - Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles
SP  - 79
EP  - 79
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11809
ER  - 

@conference{
author = "Nešić, M. D. and Filipović Tričković, Jelena and Valenta Šobot, Ana and Žakula, Jelena and Korićanac, Lela and Popović, I. and Soto, J. and Algarra, M. and Ralić, V. and Abu el Rub, Anamarija and Matijević, M. and Stepić, Milutin and Petković, M.",
year = "2023",
abstract = "Carbon Dots (CDs) are biocompatible, fluorescent, water-soluble, and stable nanoparticles with a high potential to be used for vast biomedical applications [1,2]. We explore the application of CDs produced from natural sources, black carrots, as anti-cancer and imaging agents. These nanoparticles suppress cell growth of three different cancer cell lines, cervical (HeLa), pancreatic (PANC-1), and melanoma (A375) cell lines in vitro. However, the cytotoxic effect against A375 cells stands out, with only 20% of viable cells left after treatment (Fig.1(a)), antimetastatic potential, and a selectivity index higher than two, which indicates that the efficacy against melanoma cells is significantly greater than the toxicity against non-malignant cells (MRC-5). Furthermore, after the cellular uptake, green fluorescence was visible in the cytosol of A375 cells (Fig. 1 (b)). On the other hand, the DAPI stain for DNA was visible as a blue light in the cell nucleus. Moreover, cells with a higher intensity of green fluorescence in the nucleus, Fig. 1 (c) indicated with arrows, were the cells with condensed chromatin in the mitotic phase of the cell cycle (Fig. 1 (d) and (e)), which indicates that CDs interact with chromatin and that they could be used as a marker of cells mitosis and proliferation. In summary, we have demonstrated the great anti-cancer potential of black carrot CDs, for image-guided anti-cancer therapy of melanoma that can be used to recognize cell proliferation.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade",
title = "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles",
pages = "79-79",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11809"
}

Nešić, M. D., Filipović Tričković, J., Valenta Šobot, A., Žakula, J., Korićanac, L., Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, A., Matijević, M., Stepić, M.,& Petković, M.. (2023). Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade
Belgrade : Vinča Institute of Nuclear Sciences., 79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809

Nešić MD, Filipović Tričković J, Valenta Šobot A, Žakula J, Korićanac L, Popović I, Soto J, Algarra M, Ralić V, Abu el Rub A, Matijević M, Stepić M, Petković M. Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles. in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade. 2023;:79-79.
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

Nešić, M. D., Filipović Tričković, Jelena, Valenta Šobot, Ana, Žakula, Jelena, Korićanac, Lela, Popović, I., Soto, J., Algarra, M., Ralić, V., Abu el Rub, Anamarija, Matijević, M., Stepić, Milutin, Petković, M., "Anti-cancer and imaging potential of fluorescent black carrot Carbon Dot nanoparticles" in PHOTONICA2023 : 9th International School and Conference on Photonics : book of abstracts; August 28 - September 1, 2023; Belgrade (2023):79-79,
https://hdl.handle.net/21.15107/rcub_vinar_11809 .

TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Momčilović, Miloš
AU  - Joksić, Gordana
AU  - Živković, Sanja
AU  - Ilić, Bojana
AU  - Ognjanović, Miloš
AU  - Novaković, Mirjana
AU  - Valenta-Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11376
AB  - Silver nanoparticles (AgNPs) have been recognized for widespread biological applications due to their antimicrobial and anti-inflammatory effect, especially in dentistry and for wound healing. Many features determine their beneficial or toxic potential, such as their synthesis type, size, morphology, coating, and concentration. Most synthesis types rely on the use of synthetic chemicals, which contributes to their toxicity. We present an environmentally friendly method for “green” synthesis of AgNPs from the silver target by pulsed laser ablation in liquid (PLAL) using citrate as the stabilizing agent. Since AgNPs already have many dental applications, we examined their antibacterial effect against supragingival biofilm-forming bacteria and bacterial strains known to cause resistant dental infections. Their impact on human fibroblast cells’ cytotoxicity, proliferation (measured by XTT and Ki-67 immunofluorescence), pro/antioxidant balance, and lipid peroxidation (measured by PAB and LPP) was evaluated. AgNPs1 (21 nm) and AgNPs2 (15 nm) spherical nanoparticles with good overall stability were obtained. The highest tested dose of smaller nanoparticles (AgNPs2) displays not only an effective antibacterial effect against the tested oral bacteria strains but also a pro-oxidant and cytotoxic effect on fibroblast cells. Lower doses do not affect bacterial survival but increase the cell proliferation and metabolic activity and show an antioxidative effect, suggesting that different concentrations display a substantially opposite effect. Compared to larger AgNPs1, smaller AgNPs2 possess more potent biological effects, indicating that size plays a pivotal role in their activity. Such opposite outcomes could be important for their medical application, and high concentrations could be used for the inhibition of dental biofilm formation and resistant dental infections as well as proliferative conditions, while low doses could be beneficial in the treatment of atrophic and inflammatory disorders. Finally, we showed that silver-targeted PLAL, using citrate as a stabilizing agent, produces biologically potent nanoparticles that could have many applications depending on their size and concentration.
T2  - Nanomaterials and Nanotechnology
T1  - Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects
VL  - 2023
SP  - e9854356
DO  - 10.1155/2023/9854356
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Momčilović, Miloš and Joksić, Gordana and Živković, Sanja and Ilić, Bojana and Ognjanović, Miloš and Novaković, Mirjana and Valenta-Šobot, Ana",
year = "2023",
abstract = "Silver nanoparticles (AgNPs) have been recognized for widespread biological applications due to their antimicrobial and anti-inflammatory effect, especially in dentistry and for wound healing. Many features determine their beneficial or toxic potential, such as their synthesis type, size, morphology, coating, and concentration. Most synthesis types rely on the use of synthetic chemicals, which contributes to their toxicity. We present an environmentally friendly method for “green” synthesis of AgNPs from the silver target by pulsed laser ablation in liquid (PLAL) using citrate as the stabilizing agent. Since AgNPs already have many dental applications, we examined their antibacterial effect against supragingival biofilm-forming bacteria and bacterial strains known to cause resistant dental infections. Their impact on human fibroblast cells’ cytotoxicity, proliferation (measured by XTT and Ki-67 immunofluorescence), pro/antioxidant balance, and lipid peroxidation (measured by PAB and LPP) was evaluated. AgNPs1 (21 nm) and AgNPs2 (15 nm) spherical nanoparticles with good overall stability were obtained. The highest tested dose of smaller nanoparticles (AgNPs2) displays not only an effective antibacterial effect against the tested oral bacteria strains but also a pro-oxidant and cytotoxic effect on fibroblast cells. Lower doses do not affect bacterial survival but increase the cell proliferation and metabolic activity and show an antioxidative effect, suggesting that different concentrations display a substantially opposite effect. Compared to larger AgNPs1, smaller AgNPs2 possess more potent biological effects, indicating that size plays a pivotal role in their activity. Such opposite outcomes could be important for their medical application, and high concentrations could be used for the inhibition of dental biofilm formation and resistant dental infections as well as proliferative conditions, while low doses could be beneficial in the treatment of atrophic and inflammatory disorders. Finally, we showed that silver-targeted PLAL, using citrate as a stabilizing agent, produces biologically potent nanoparticles that could have many applications depending on their size and concentration.",
journal = "Nanomaterials and Nanotechnology",
title = "Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects",
volume = "2023",
pages = "e9854356",
doi = "10.1155/2023/9854356"
}

Filipović Tričković, J. G., Momčilović, M., Joksić, G., Živković, S., Ilić, B., Ognjanović, M., Novaković, M.,& Valenta-Šobot, A.. (2023). Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects. in Nanomaterials and Nanotechnology, 2023, e9854356.
https://doi.org/10.1155/2023/9854356

Filipović Tričković JG, Momčilović M, Joksić G, Živković S, Ilić B, Ognjanović M, Novaković M, Valenta-Šobot A. Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects. in Nanomaterials and Nanotechnology. 2023;2023:e9854356.
doi:10.1155/2023/9854356 .

Filipović Tričković, Jelena G., Momčilović, Miloš, Joksić, Gordana, Živković, Sanja, Ilić, Bojana, Ognjanović, Miloš, Novaković, Mirjana, Valenta-Šobot, Ana, "Laser Ablated Citrate-Stabilized Silver Nanoparticles Display Size and Concentration Dependant Biological Effects" in Nanomaterials and Nanotechnology, 2023 (2023):e9854356,
https://doi.org/10.1155/2023/9854356 . .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Šaponjić, Aleksandra
AU  - Baščarević, Zvezdana
AU  - Jovanović, Ivan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11280
AB  - Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using
Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica.
The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al.
Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained
after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher
temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled
water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres
of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive
X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and
Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic
effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from
particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated
with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical
damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium
had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.
PB  - Association of Metallurgical Engineers of Serbia (AMES)
C3  - MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina
T1  - Preliminary cytotoxicity testing of newly synthesised SBA-15 material
SP  - 60
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11280
ER  - 

@conference{
author = "Kokunešoski, Maja and Šaponjić, Aleksandra and Baščarević, Zvezdana and Jovanović, Ivan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana",
year = "2023",
abstract = "Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using
Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica.
The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al.
Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained
after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher
temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled
water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres
of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive
X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and
Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic
effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from
particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated
with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical
damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium
had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.",
publisher = "Association of Metallurgical Engineers of Serbia (AMES)",
journal = "MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina",
title = "Preliminary cytotoxicity testing of newly synthesised SBA-15 material",
pages = "60",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11280"
}

Kokunešoski, M., Šaponjić, A., Baščarević, Z., Jovanović, I., Filipović Tričković, J. G.,& Valenta-Šobot, A.. (2023). Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina
Association of Metallurgical Engineers of Serbia (AMES)., 60.
https://hdl.handle.net/21.15107/rcub_vinar_11280

Kokunešoski M, Šaponjić A, Baščarević Z, Jovanović I, Filipović Tričković JG, Valenta-Šobot A. Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina. 2023;:60.
https://hdl.handle.net/21.15107/rcub_vinar_11280 .

Kokunešoski, Maja, Šaponjić, Aleksandra, Baščarević, Zvezdana, Jovanović, Ivan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, "Preliminary cytotoxicity testing of newly synthesised SBA-15 material" in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina (2023):60,
https://hdl.handle.net/21.15107/rcub_vinar_11280 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Drakulić, Dunja
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Momić, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11582
AB  - Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
T1  - Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells
SP  - 47
EP  - 47
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11582
ER  - 

@conference{
author = "Valenta Šobot, Ana and Filipović Tričković, Jelena and Drakulić, Dunja and Leskovac, Andreja and Petrović, Sandra and Momić, Tatjana",
year = "2022",
abstract = "Sweroside (Sw) is a secondary metabolite commonly found in plants belonging to the Gentianaceae family [1]. This iridoid compound is well-known for its anti-inflammatory [2], antidiabetic [3] and antitumor properties [4], which have been studied in pathological model systems. In transformed cell lines, Sw displays an antitumor effect by apoptosis activation [5]. Since healthy cells are also exposed to cancer therapy applied in vivo, our goal was to determine Sw’s cytotoxic concentration in primary human peripheral blood mononuclear cells (PBMCs) after 48 h of treatment with a range of concentration from 20 μM to 130 μM Sw in vitro, and to analyze whether the cytotoxic effect was due to activated apoptosis. According to the obtained results of the trypan blue dye exclusion test, 48 h of treatment with 50 μM Sw and higher concentrations led to a significant decrease in cell number. The DNA fragmentation assay indicated that following 50 μM Sw treatment, cells are dying in an apoptosis-like manner since the level of DNA fragments was 3.5 times higher than in the untreated control. The type of cell death was confirmed by immunoblot analysis of apoptosis- specific protein markers, which revealed the elevation of cleaved caspase-3 and PARP1 89 kDa fragments. Our findings showed that like in transformed cell lines, Sw in healthy cells can also activate apoptosis. A potential difference in sensitivity to Sw treatment between healthy and transformed cells could justify Sw treatment in anticancer therapy.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts",
title = "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells",
pages = "47-47",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11582"
}

Valenta Šobot, A., Filipović Tričković, J., Drakulić, D., Leskovac, A., Petrović, S.,& Momić, T.. (2022). Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
Belgrade : University of Belgrade, Faculty of Agriculture., 47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582

Valenta Šobot A, Filipović Tričković J, Drakulić D, Leskovac A, Petrović S, Momić T. Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts. 2022;:47-47.
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

Valenta Šobot, Ana, Filipović Tričković, Jelena, Drakulić, Dunja, Leskovac, Andreja, Petrović, Sandra, Momić, Tatjana, "Sweroside displays a cytotoxic effect by activating apoptosis in human peripheral blood mononuclear cells" in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts (2022):47-47,
https://hdl.handle.net/21.15107/rcub_vinar_11582 .

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Momić, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11583
AB  - Quercetin (Q) is one of the most common and well researched antioxidant flavonoids, which usually occurs in plant-based foods, and medicinal plants. It was shown that quercetin exerts many beneficial effects on human health, including prevention of cancer and heart diseases. Quercetin was found to be toxic toward various types of cancer cell, still, at higher concentrations it was also shown toxic toward normal human cells [1,2]. One of the approaches to overcome this shortcoming offers nanotechnology which enables the novel perspective of phytochemical usage in contemporary medicine [3]. The strategy of binding quercetin to the gold nanoparticles during their synthesis was used, which resulted in quercetin capped gold nanoparticles (NPQ) [4]. Trypan blue exclusion test [5] was used to evaluate peripheral blood mononuclear cells (PBMC) viability after their exposure to either NPQ or free Q during 24, 48 and 72 h, at 37 °C, in the range of quercetin concentrations from 5 to 50 μg/mL. A significant reduction in the cell count was observed in PBMC cultures treated with 10, 20, and 50 μg/mL of free Q, for all exposure times. The treatments of increasing concentrations and exposure times lowered the cells viability, resulting in 63% of the viable cells, following 72 h of the treatment with 50 μg/mL of free Q. Although NPQ treatments affected the cells viability in a concentration- and time-dependent manner the treatment with 50 μg/mL of NPQ for 72 h, had a milder effect on PBMC cultures than free Q, resulting in 81% of the viable cells (Figure 1). According to the obtained results, NPQ were shown less toxic toward PBMC than free Q.
PB  - Belgrade : University of Belgrade, Faculty of Agriculture
C3  - 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
T1  - Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells
SP  - 64
EP  - 64
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11583
ER  - 

@conference{
author = "Valenta Šobot, Ana and Filipović Tričković, Jelena and Leskovac, Andreja and Petrović, Sandra and Momić, Tatjana",
year = "2022",
abstract = "Quercetin (Q) is one of the most common and well researched antioxidant flavonoids, which usually occurs in plant-based foods, and medicinal plants. It was shown that quercetin exerts many beneficial effects on human health, including prevention of cancer and heart diseases. Quercetin was found to be toxic toward various types of cancer cell, still, at higher concentrations it was also shown toxic toward normal human cells [1,2]. One of the approaches to overcome this shortcoming offers nanotechnology which enables the novel perspective of phytochemical usage in contemporary medicine [3]. The strategy of binding quercetin to the gold nanoparticles during their synthesis was used, which resulted in quercetin capped gold nanoparticles (NPQ) [4]. Trypan blue exclusion test [5] was used to evaluate peripheral blood mononuclear cells (PBMC) viability after their exposure to either NPQ or free Q during 24, 48 and 72 h, at 37 °C, in the range of quercetin concentrations from 5 to 50 μg/mL. A significant reduction in the cell count was observed in PBMC cultures treated with 10, 20, and 50 μg/mL of free Q, for all exposure times. The treatments of increasing concentrations and exposure times lowered the cells viability, resulting in 63% of the viable cells, following 72 h of the treatment with 50 μg/mL of free Q. Although NPQ treatments affected the cells viability in a concentration- and time-dependent manner the treatment with 50 μg/mL of NPQ for 72 h, had a milder effect on PBMC cultures than free Q, resulting in 81% of the viable cells (Figure 1). According to the obtained results, NPQ were shown less toxic toward PBMC than free Q.",
publisher = "Belgrade : University of Belgrade, Faculty of Agriculture",
journal = "1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts",
title = "Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells",
pages = "64-64",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11583"
}

Valenta Šobot, A., Filipović Tričković, J., Leskovac, A., Petrović, S.,& Momić, T.. (2022). Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts
Belgrade : University of Belgrade, Faculty of Agriculture., 64-64.
https://hdl.handle.net/21.15107/rcub_vinar_11583

Valenta Šobot A, Filipović Tričković J, Leskovac A, Petrović S, Momić T. Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells. in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts. 2022;:64-64.
https://hdl.handle.net/21.15107/rcub_vinar_11583 .

Valenta Šobot, Ana, Filipović Tričković, Jelena, Leskovac, Andreja, Petrović, Sandra, Momić, Tatjana, "Nanotechnology approach for diminishing quercetin toxicity toward peripheral blood mononuclear cells" in 1-EuSPMF - 1st European symposium on phytochemicals in medicine and food : Book of abstracts (2022):64-64,
https://hdl.handle.net/21.15107/rcub_vinar_11583 .

TY  - CONF
AU  - Tasić, T.
AU  - Milanković, Vedran
AU  - Kokunešoski, Maja
AU  - Šaponjić, Aleksandra
AU  - Valenta-Šobot, Ana
AU  - Grce, Ana
AU  - Lazarević-Pašti, Tamara
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11285
AB  - Organophosphate pesticides are primarily used in agriculture despite their high
neurotoxicity to humans and the environment. Malathion is used in agriculture as a means
of controlling the development of pests, but also in the protection of public health, to
control mosquitoes and lice. However, this organophosphate compound is hazardous to
human health. We used wood-waste-derived activated porous carbon material as an
adsorbent for malathion removal from water. The kinetics of the malathion adsorption
process on investigated carbon material at different temperatures was studied. The results
showed that the experimental data are a good fit with the pseudo-second-order model.
The calculated rate constants were 0.009 and 0.007 g mg−1 min−1
at 20 and 40 °C,
respectively. The equilibrium was reached after 60 minutes at both investigated
temperatures. It was shown that 1 g of the studied material could adsorb up to 186.22 mg
of malathion at 20 °C.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts
T1  - Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water
SP  - 135
EP  - 135
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11285
ER  - 

@conference{
author = "Tasić, T. and Milanković, Vedran and Kokunešoski, Maja and Šaponjić, Aleksandra and Valenta-Šobot, Ana and Grce, Ana and Lazarević-Pašti, Tamara",
year = "2022",
abstract = "Organophosphate pesticides are primarily used in agriculture despite their high
neurotoxicity to humans and the environment. Malathion is used in agriculture as a means
of controlling the development of pests, but also in the protection of public health, to
control mosquitoes and lice. However, this organophosphate compound is hazardous to
human health. We used wood-waste-derived activated porous carbon material as an
adsorbent for malathion removal from water. The kinetics of the malathion adsorption
process on investigated carbon material at different temperatures was studied. The results
showed that the experimental data are a good fit with the pseudo-second-order model.
The calculated rate constants were 0.009 and 0.007 g mg−1 min−1
at 20 and 40 °C,
respectively. The equilibrium was reached after 60 minutes at both investigated
temperatures. It was shown that 1 g of the studied material could adsorb up to 186.22 mg
of malathion at 20 °C.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts",
title = "Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water",
pages = "135-135",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11285"
}

Tasić, T., Milanković, V., Kokunešoski, M., Šaponjić, A., Valenta-Šobot, A., Grce, A.,& Lazarević-Pašti, T.. (2022). Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts
Society of Physical Chemists of Serbia., 135-135.
https://hdl.handle.net/21.15107/rcub_vinar_11285

Tasić T, Milanković V, Kokunešoski M, Šaponjić A, Valenta-Šobot A, Grce A, Lazarević-Pašti T. Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water. in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts. 2022;:135-135.
https://hdl.handle.net/21.15107/rcub_vinar_11285 .

Tasić, T., Milanković, Vedran, Kokunešoski, Maja, Šaponjić, Aleksandra, Valenta-Šobot, Ana, Grce, Ana, Lazarević-Pašti, Tamara, "Wood-Waste-Derived Activated Porous Carbon Material for Pesticide Removal From Water" in PHYSICAL CHEMISTRY 2022 : 16th International Conference on Fundamental and Applied Aspects of Physical Chemistry : Book of abstracts (2022):135-135,
https://hdl.handle.net/21.15107/rcub_vinar_11285 .

TY  - JOUR
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Joksić, Ivana
AU  - Joksić, Gordana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10258
AB  - AbstractBiološki učinci ionizirajućega zračenja (IZ) pripisuju se oštećenjima DNA i indirektnim učincima kroz povećanu proizvodnju reaktivnih vrsta kisika. Iako se telomere rabe kao pokazatelji radioosjetljivosti, o njihovu ponašanju kao odgovoru na ionizirajuće zračenje u uvjetima profesionalne izloženosti i dalje se raspravlja. U ovom radu željeli smo istražiti duljinu i strukturu telomera u bolničkih radnika koji su profesionalno izloženi ionizirajućem zračenju te povezati te nalaze s oksidacijskim biomolekulama i kromosomskim aberacijama. Uzorci krvi izloženih ispitanika i zdravih kontrola uzeti su za analizu tijekom rutinskoga godišnjeg zdravstvenog pregleda. Osim kromosomskih aberacija, u uzorcima plazme izmjereni su i parametri oksidacijskoga stresa [prooksidacijska/antioksidacijska ravnoteža (PAB), lipidna peroksidacija i 8-okso-dG], a procjena duljine i strukture telomera provedena je metodom Q-FISH na metafaznim kromosomima. Analiza kromosomskih aberacija pokazala je da od 34 ispitanika njih 14 ima kromosomske aberacije (skupina 1), a 20 nije imalo aberacije (skupina 2). Nije bilo značajne razlike u spolu ili dobi ni u duljini telomera između skupina. Međutim, incidencija lomljivih telomera bila je značajno veća u objema skupinama ispitanika izloženih IZ-u u usporedbi s kontrolnim ispitanicima. Produkti peroksidacije lipida i 8-okso-dG također su bili značajno viši u objema skupinama. Učestalost lomljivih telomera u pozitivnoj je korelaciji (statistički značajna) s razinama 8-okso-dG u
T2  - Archives of Industrial Hygiene and Toxicology
T1  - Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation
VL  - 73
IS  - 1
SP  - 23
EP  - 30
DO  - 10.2478/aiht-2022-73-3609
ER  - 

@article{
author = "Filipović Tričković, Jelena G. and Valenta-Šobot, Ana and Joksić, Ivana and Joksić, Gordana",
year = "2022",
abstract = "AbstractBiološki učinci ionizirajućega zračenja (IZ) pripisuju se oštećenjima DNA i indirektnim učincima kroz povećanu proizvodnju reaktivnih vrsta kisika. Iako se telomere rabe kao pokazatelji radioosjetljivosti, o njihovu ponašanju kao odgovoru na ionizirajuće zračenje u uvjetima profesionalne izloženosti i dalje se raspravlja. U ovom radu željeli smo istražiti duljinu i strukturu telomera u bolničkih radnika koji su profesionalno izloženi ionizirajućem zračenju te povezati te nalaze s oksidacijskim biomolekulama i kromosomskim aberacijama. Uzorci krvi izloženih ispitanika i zdravih kontrola uzeti su za analizu tijekom rutinskoga godišnjeg zdravstvenog pregleda. Osim kromosomskih aberacija, u uzorcima plazme izmjereni su i parametri oksidacijskoga stresa [prooksidacijska/antioksidacijska ravnoteža (PAB), lipidna peroksidacija i 8-okso-dG], a procjena duljine i strukture telomera provedena je metodom Q-FISH na metafaznim kromosomima. Analiza kromosomskih aberacija pokazala je da od 34 ispitanika njih 14 ima kromosomske aberacije (skupina 1), a 20 nije imalo aberacije (skupina 2). Nije bilo značajne razlike u spolu ili dobi ni u duljini telomera između skupina. Međutim, incidencija lomljivih telomera bila je značajno veća u objema skupinama ispitanika izloženih IZ-u u usporedbi s kontrolnim ispitanicima. Produkti peroksidacije lipida i 8-okso-dG također su bili značajno viši u objema skupinama. Učestalost lomljivih telomera u pozitivnoj je korelaciji (statistički značajna) s razinama 8-okso-dG u",
journal = "Archives of Industrial Hygiene and Toxicology",
title = "Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation",
volume = "73",
number = "1",
pages = "23-30",
doi = "10.2478/aiht-2022-73-3609"
}

Filipović Tričković, J. G., Valenta-Šobot, A., Joksić, I.,& Joksić, G.. (2022). Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation. in Archives of Industrial Hygiene and Toxicology, 73(1), 23-30.
https://doi.org/10.2478/aiht-2022-73-3609

Filipović Tričković JG, Valenta-Šobot A, Joksić I, Joksić G. Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation. in Archives of Industrial Hygiene and Toxicology. 2022;73(1):23-30.
doi:10.2478/aiht-2022-73-3609 .

Filipović Tričković, Jelena G., Valenta-Šobot, Ana, Joksić, Ivana, Joksić, Gordana, "Telomere fragility in radiology workers occupationally exposed to low doses of ionising radiation" in Archives of Industrial Hygiene and Toxicology, 73, no. 1 (2022):23-30,
https://doi.org/10.2478/aiht-2022-73-3609 . .

TY  - CONF
AU  - Butulija, Svetlana
AU  - Filipović Tričković, Jelena G.
AU  - Valenta, Ana
AU  - Radovanović, Željko
AU  - Ćetenović, Bojana
AU  - Zmejkoski, Danica
AU  - Todorović, Bratislav
AU  - Matović, Branko
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13331
AB  - Bacterial cellulose (BC) is a promising natural polymer with a range of characteristics such as biocompatibility, microporosity, transparency, conformability, elasticity, the ability to preserve a moist environment, as well as absorb exudates in wounds. That is why BC is an attractive material in biomedical applications, especially in skin tissue repairing, but its lack of antimicrobial activity limits its performance. To overcome this shortage, BC was combined with cerium (IV)-oxide (CeO2) nanoparticles. Series of bacterial cellulose-CeO2 (BC-CeO2) composite materials were prepared by soaking bacterial cellulose in different concentrations of cerium-oxide nanoparticle water suspensions. All composites had antibacterial activity, and toxicity, as well as genotoxicity of BC- CeO2, had been examined. The BC-CeO2/0.1M composite stands out as it did not show the cytotoxic, nor the genotoxic effect on peripheral blood lymphocytes, and additionally protected the cells from the genotoxic effect in H2O2 treated cultures. Redox parameters in blood plasma samples were concentration and time-dependent for PAB and LPP assay. The results of the survey imply that BC- CeO2 nanocomposite might be a promising wound healing material.
PB  - Belgrade : Materials Research Society of Serbia – MRS-Serbia
C3  - YUCOMAT 2021 : 22nd Annual Conference YUCOMAT 2021 : Programme and the Book of abstracts
T1  - Synthesis, characterization and possible application of bacterial cellulose-ceria composite
SP  - 84
EP  - 84
ER  - 

@conference{
author = "Butulija, Svetlana and Filipović Tričković, Jelena G. and Valenta, Ana and Radovanović, Željko and Ćetenović, Bojana and Zmejkoski, Danica and Todorović, Bratislav and Matović, Branko",
year = "2021",
abstract = "Bacterial cellulose (BC) is a promising natural polymer with a range of characteristics such as biocompatibility, microporosity, transparency, conformability, elasticity, the ability to preserve a moist environment, as well as absorb exudates in wounds. That is why BC is an attractive material in biomedical applications, especially in skin tissue repairing, but its lack of antimicrobial activity limits its performance. To overcome this shortage, BC was combined with cerium (IV)-oxide (CeO2) nanoparticles. Series of bacterial cellulose-CeO2 (BC-CeO2) composite materials were prepared by soaking bacterial cellulose in different concentrations of cerium-oxide nanoparticle water suspensions. All composites had antibacterial activity, and toxicity, as well as genotoxicity of BC- CeO2, had been examined. The BC-CeO2/0.1M composite stands out as it did not show the cytotoxic, nor the genotoxic effect on peripheral blood lymphocytes, and additionally protected the cells from the genotoxic effect in H2O2 treated cultures. Redox parameters in blood plasma samples were concentration and time-dependent for PAB and LPP assay. The results of the survey imply that BC- CeO2 nanocomposite might be a promising wound healing material.",
publisher = "Belgrade : Materials Research Society of Serbia – MRS-Serbia",
journal = "YUCOMAT 2021 : 22nd Annual Conference YUCOMAT 2021 : Programme and the Book of abstracts",
title = "Synthesis, characterization and possible application of bacterial cellulose-ceria composite",
pages = "84-84"
}

Butulija, S., Filipović Tričković, J. G., Valenta, A., Radovanović, Ž., Ćetenović, B., Zmejkoski, D., Todorović, B.,& Matović, B.. (2021). Synthesis, characterization and possible application of bacterial cellulose-ceria composite. in YUCOMAT 2021 : 22nd Annual Conference YUCOMAT 2021 : Programme and the Book of abstracts
Belgrade : Materials Research Society of Serbia – MRS-Serbia., 84-84.

Butulija S, Filipović Tričković JG, Valenta A, Radovanović Ž, Ćetenović B, Zmejkoski D, Todorović B, Matović B. Synthesis, characterization and possible application of bacterial cellulose-ceria composite. in YUCOMAT 2021 : 22nd Annual Conference YUCOMAT 2021 : Programme and the Book of abstracts. 2021;:84-84..

Butulija, Svetlana, Filipović Tričković, Jelena G., Valenta, Ana, Radovanović, Željko, Ćetenović, Bojana, Zmejkoski, Danica, Todorović, Bratislav, Matović, Branko, "Synthesis, characterization and possible application of bacterial cellulose-ceria composite" in YUCOMAT 2021 : 22nd Annual Conference YUCOMAT 2021 : Programme and the Book of abstracts (2021):84-84.

TY  - CONF
AU  - Valenta Šobot, Ana
AU  - Drakulić, Dunja
AU  - Savić, Jasmina
AU  - Joksić, Gordana
AU  - Filipović-Tričković, Jelena
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13332
PB  - Belgrade : Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia
C3  - Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
T1  - Yellow Gentian Root Extract and its Monoterpene Compounds Exhibit Anticancer Potential
SP  - 123
EP  - 126
ER  - 

@conference{
author = "Valenta Šobot, Ana and Drakulić, Dunja and Savić, Jasmina and Joksić, Gordana and Filipović-Tričković, Jelena",
year = "2021",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia",
journal = "Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021",
title = "Yellow Gentian Root Extract and its Monoterpene Compounds Exhibit Anticancer Potential",
pages = "123-126"
}

Valenta Šobot, A., Drakulić, D., Savić, J., Joksić, G.,& Filipović-Tričković, J.. (2021). Yellow Gentian Root Extract and its Monoterpene Compounds Exhibit Anticancer Potential. in Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021
Belgrade : Vinča Institute of Nuclear Sciences - National Institute of the Republic of Serbia., 123-126.

Valenta Šobot A, Drakulić D, Savić J, Joksić G, Filipović-Tričković J. Yellow Gentian Root Extract and its Monoterpene Compounds Exhibit Anticancer Potential. in Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021. 2021;:123-126..

Valenta Šobot, Ana, Drakulić, Dunja, Savić, Jasmina, Joksić, Gordana, Filipović-Tričković, Jelena, "Yellow Gentian Root Extract and its Monoterpene Compounds Exhibit Anticancer Potential" in Proceedings - 7th Workshop Specific Methods for Food Safety and Quality, September 22nd, 2021, Belgrade, Serbia [is an online satellite event of] 15th International Conference on Fundamental and Applied Aspects of Physical Chemistry - Physical Chemistry 2021 (2021):123-126.

TY  - JOUR
AU  - Matijević, Milica
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Radoičić, Marija B.
AU  - Liang, Xinyue
AU  - Mi, Lan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Stanković, Maja N.
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9908
AB  - In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.
T2  - Photochemical and Photobiological Sciences
T1  - Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles
VL  - 20
IS  - 8
SP  - 1087
EP  - 1098
DO  - 10.1007/s43630-021-00082-2
ER  - 

@article{
author = "Matijević, Milica and Žakula, Jelena and Korićanac, Lela and Radoičić, Marija B. and Liang, Xinyue and Mi, Lan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana and Stanković, Maja N. and Nakarada, Đura and Mojović, Miloš and Petković, Marijana and Stepić, Milutin and Nešić, Maja D.",
year = "2021",
abstract = "In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.",
journal = "Photochemical and Photobiological Sciences",
title = "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles",
volume = "20",
number = "8",
pages = "1087-1098",
doi = "10.1007/s43630-021-00082-2"
}

Matijević, M., Žakula, J., Korićanac, L., Radoičić, M. B., Liang, X., Mi, L., Filipović Tričković, J. G., Valenta-Šobot, A., Stanković, M. N., Nakarada, Đ., Mojović, M., Petković, M., Stepić, M.,& Nešić, M. D.. (2021). Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences, 20(8), 1087-1098.
https://doi.org/10.1007/s43630-021-00082-2

Matijević M, Žakula J, Korićanac L, Radoičić MB, Liang X, Mi L, Filipović Tričković JG, Valenta-Šobot A, Stanković MN, Nakarada Đ, Mojović M, Petković M, Stepić M, Nešić MD. Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences. 2021;20(8):1087-1098.
doi:10.1007/s43630-021-00082-2 .

Matijević, Milica, Žakula, Jelena, Korićanac, Lela, Radoičić, Marija B., Liang, Xinyue, Mi, Lan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, Stanković, Maja N., Nakarada, Đura, Mojović, Miloš, Petković, Marijana, Stepić, Milutin, Nešić, Maja D., "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles" in Photochemical and Photobiological Sciences, 20, no. 8 (2021):1087-1098,
https://doi.org/10.1007/s43630-021-00082-2 . .

TY  - CONF
AU  - Valenta-Šobot, Ana
AU  - Filipović Tričković, Jelena G.
AU  - Šaponjić, Aleksandra
AU  - Ilić, Svetlana
AU  - Grce, Ana
AU  - Katnić, Đurica
AU  - Kokunešoski, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11293
AB  - Mesoporous silica renders chemical and mechanical stability under biological conditions, but due to its physicochemical properties, could be potentially harmful to exposed cells. The aim of the current study was to estimate SBA-15 (concentration of 50, 100 and 250 µg/mL) impact on human peripheral blood mononuclear cells to induce cyto- and genotoxicity in vitro after 72 h treatment, as well as lipid peroxidation in serum samples ex vivo. SBA-15 mesoporous silica treatment impact on cell viability was performed by XTT assay, lipid peroxidation was estimated by determining malondialdehyde and 4-hydroxyalkenals levels and genotoxicity by micronucleus assay. SBA-15 treatments displayed genotoxic potential at the lowest concentration, while highest tested concentration led to decrease of cell viability and increase of lipid peroxidation. Chemical modification of SBA-15 material that could influence its physicochemical properties could be useful way to lower its toxicity.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
T1  - Mesoporous silica decrease cell viability in vitro in dose dependent manner
VL  - II
SP  - 442
EP  - 444
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11293
ER  - 

@conference{
author = "Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Šaponjić, Aleksandra and Ilić, Svetlana and Grce, Ana and Katnić, Đurica and Kokunešoski, Maja",
year = "2021",
abstract = "Mesoporous silica renders chemical and mechanical stability under biological conditions, but due to its physicochemical properties, could be potentially harmful to exposed cells. The aim of the current study was to estimate SBA-15 (concentration of 50, 100 and 250 µg/mL) impact on human peripheral blood mononuclear cells to induce cyto- and genotoxicity in vitro after 72 h treatment, as well as lipid peroxidation in serum samples ex vivo. SBA-15 mesoporous silica treatment impact on cell viability was performed by XTT assay, lipid peroxidation was estimated by determining malondialdehyde and 4-hydroxyalkenals levels and genotoxicity by micronucleus assay. SBA-15 treatments displayed genotoxic potential at the lowest concentration, while highest tested concentration led to decrease of cell viability and increase of lipid peroxidation. Chemical modification of SBA-15 material that could influence its physicochemical properties could be useful way to lower its toxicity.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry",
title = "Mesoporous silica decrease cell viability in vitro in dose dependent manner",
volume = "II",
pages = "442-444",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11293"
}

Valenta-Šobot, A., Filipović Tričković, J. G., Šaponjić, A., Ilić, S., Grce, A., Katnić, Đ.,& Kokunešoski, M.. (2021). Mesoporous silica decrease cell viability in vitro in dose dependent manner. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., II, 442-444.
https://hdl.handle.net/21.15107/rcub_vinar_11293

Valenta-Šobot A, Filipović Tričković JG, Šaponjić A, Ilić S, Grce A, Katnić Đ, Kokunešoski M. Mesoporous silica decrease cell viability in vitro in dose dependent manner. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry. 2021;II:442-444.
https://hdl.handle.net/21.15107/rcub_vinar_11293 .

Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Šaponjić, Aleksandra, Ilić, Svetlana, Grce, Ana, Katnić, Đurica, Kokunešoski, Maja, "Mesoporous silica decrease cell viability in vitro in dose dependent manner" in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry, II (2021):442-444,
https://hdl.handle.net/21.15107/rcub_vinar_11293 .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Baščarević, Zvezdana
AU  - Ilić, Svetlana
AU  - Valenta-Šobot, Ana
AU  - Grce, Ana
AU  - Pošarac-Marković, Milica
AU  - Šaponjić, Đorđe
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11290
AB  - The template method synthesis of both SBA-15 materials with elongated and spherical particles was performed using a surfactant Pluronic P123. The HCl (p.a.) was used to synthesize material with elongated particles connected in chain structures grouped into shapes resembling sheaves of wheat. In the synthesis of spherical SBA-15 with diameters ranging from 0.5 to 2 μm, a spent HCl solution which was obtained after chemical treatment of clay was used where the dominant presence of the spheres was confirmed by the SEM method. In addition to the methods mentioned above, XRD, EDS and FTIR methods characterize SBA-15 materials.
PB  - Society of Physical Chemists of Serbia
C3  - PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
T1  - The influence of hydrochloric acid on the features of SBA-15 particles
VL  - II
SP  - 438
EP  - 441
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11290
ER  - 

@conference{
author = "Kokunešoski, Maja and Baščarević, Zvezdana and Ilić, Svetlana and Valenta-Šobot, Ana and Grce, Ana and Pošarac-Marković, Milica and Šaponjić, Đorđe",
year = "2021",
abstract = "The template method synthesis of both SBA-15 materials with elongated and spherical particles was performed using a surfactant Pluronic P123. The HCl (p.a.) was used to synthesize material with elongated particles connected in chain structures grouped into shapes resembling sheaves of wheat. In the synthesis of spherical SBA-15 with diameters ranging from 0.5 to 2 μm, a spent HCl solution which was obtained after chemical treatment of clay was used where the dominant presence of the spheres was confirmed by the SEM method. In addition to the methods mentioned above, XRD, EDS and FTIR methods characterize SBA-15 materials.",
publisher = "Society of Physical Chemists of Serbia",
journal = "PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry",
title = "The influence of hydrochloric acid on the features of SBA-15 particles",
volume = "II",
pages = "438-441",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11290"
}

Kokunešoski, M., Baščarević, Z., Ilić, S., Valenta-Šobot, A., Grce, A., Pošarac-Marković, M.,& Šaponjić, Đ.. (2021). The influence of hydrochloric acid on the features of SBA-15 particles. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., II, 438-441.
https://hdl.handle.net/21.15107/rcub_vinar_11290

Kokunešoski M, Baščarević Z, Ilić S, Valenta-Šobot A, Grce A, Pošarac-Marković M, Šaponjić Đ. The influence of hydrochloric acid on the features of SBA-15 particles. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry. 2021;II:438-441.
https://hdl.handle.net/21.15107/rcub_vinar_11290 .

Kokunešoski, Maja, Baščarević, Zvezdana, Ilić, Svetlana, Valenta-Šobot, Ana, Grce, Ana, Pošarac-Marković, Milica, Šaponjić, Đorđe, "The influence of hydrochloric acid on the features of SBA-15 particles" in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry, II (2021):438-441,
https://hdl.handle.net/21.15107/rcub_vinar_11290 .

TY  - CONF
AU  - Valenta-Šobot, Ana
AU  - Filipović Tričković, Jelena G.
AU  - Šaponjić, Aleksandra
AU  - Grce, Ana
AU  - Kokunešoski, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11310
PB  - Academy of Sciences and Arts of the Republic of Srpska
C3  - „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
T1  - Prolonged exposure to mesoporous silica decrease cell viability in vitro
SP  - 53
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11310
ER  - 

@conference{
author = "Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Šaponjić, Aleksandra and Grce, Ana and Kokunešoski, Maja",
year = "2021",
publisher = "Academy of Sciences and Arts of the Republic of Srpska",
journal = "„Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts",
title = "Prolonged exposure to mesoporous silica decrease cell viability in vitro",
pages = "53",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11310"
}

Valenta-Šobot, A., Filipović Tričković, J. G., Šaponjić, A., Grce, A.,& Kokunešoski, M.. (2021). Prolonged exposure to mesoporous silica decrease cell viability in vitro. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
Academy of Sciences and Arts of the Republic of Srpska., 53.
https://hdl.handle.net/21.15107/rcub_vinar_11310

Valenta-Šobot A, Filipović Tričković JG, Šaponjić A, Grce A, Kokunešoski M. Prolonged exposure to mesoporous silica decrease cell viability in vitro. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts. 2021;:53.
https://hdl.handle.net/21.15107/rcub_vinar_11310 .

Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Šaponjić, Aleksandra, Grce, Ana, Kokunešoski, Maja, "Prolonged exposure to mesoporous silica decrease cell viability in vitro" in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts (2021):53,
https://hdl.handle.net/21.15107/rcub_vinar_11310 .

TY  - CONF
AU  - Katnić, Đurica
AU  - Kokunešoski, Maja
AU  - Šaponjić, Aleksandra
AU  - Valenta-Šobot, Ana
AU  - Pošarac-Marković, Milica
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11308
PB  - Academy of Sciences and Arts of the Republic of Srpska
C3  - „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
T1  - Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution
SP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11308
ER  - 

@conference{
author = "Katnić, Đurica and Kokunešoski, Maja and Šaponjić, Aleksandra and Valenta-Šobot, Ana and Pošarac-Marković, Milica",
year = "2021",
publisher = "Academy of Sciences and Arts of the Republic of Srpska",
journal = "„Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts",
title = "Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution",
pages = "49",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11308"
}

Katnić, Đ., Kokunešoski, M., Šaponjić, A., Valenta-Šobot, A.,& Pošarac-Marković, M.. (2021). Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
Academy of Sciences and Arts of the Republic of Srpska., 49.
https://hdl.handle.net/21.15107/rcub_vinar_11308

Katnić Đ, Kokunešoski M, Šaponjić A, Valenta-Šobot A, Pošarac-Marković M. Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts. 2021;:49.
https://hdl.handle.net/21.15107/rcub_vinar_11308 .

Katnić, Đurica, Kokunešoski, Maja, Šaponjić, Aleksandra, Valenta-Šobot, Ana, Pošarac-Marković, Milica, "Influence of synthesis conditions on adsorption capacity of SBA-15/alginate adsorbent for removal ions of nickel(II) and lead(II) from aqueous solution" in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts (2021):49,
https://hdl.handle.net/21.15107/rcub_vinar_11308 .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Baščarević, Zvezdana
AU  - Ilić, Svetlana
AU  - Valenta-Šobot, Ana
AU  - Šaponjić, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11306
PB  - Academy of Sciences and Arts of the Republic of Srpska
C3  - „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
T1  - Synthesis and characterisation of SBA-15 with spherical particles
SP  - 48
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11306
ER  - 

@conference{
author = "Kokunešoski, Maja and Baščarević, Zvezdana and Ilić, Svetlana and Valenta-Šobot, Ana and Šaponjić, Aleksandra",
year = "2021",
publisher = "Academy of Sciences and Arts of the Republic of Srpska",
journal = "„Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts",
title = "Synthesis and characterisation of SBA-15 with spherical particles",
pages = "48",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11306"
}

Kokunešoski, M., Baščarević, Z., Ilić, S., Valenta-Šobot, A.,& Šaponjić, A.. (2021). Synthesis and characterisation of SBA-15 with spherical particles. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts
Academy of Sciences and Arts of the Republic of Srpska., 48.
https://hdl.handle.net/21.15107/rcub_vinar_11306

Kokunešoski M, Baščarević Z, Ilić S, Valenta-Šobot A, Šaponjić A. Synthesis and characterisation of SBA-15 with spherical particles. in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts. 2021;:48.
https://hdl.handle.net/21.15107/rcub_vinar_11306 .

Kokunešoski, Maja, Baščarević, Zvezdana, Ilić, Svetlana, Valenta-Šobot, Ana, Šaponjić, Aleksandra, "Synthesis and characterisation of SBA-15 with spherical particles" in „Contemporary Materials 2021“ : 14th International Scientific Conference : programme and book of abstracts (2021):48,
https://hdl.handle.net/21.15107/rcub_vinar_11306 .

TY  - CONF
AU  - Kokunešoski, Maja
AU  - Bašćarević, Zvezdana
AU  - Ilić, Svetlana
AU  - Valenta-Šobot, Ana
AU  - Grce, Ana
AU  - Pošarac-Marković, Milica B.
AU  - Šaponjić, Aleksandra
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10799
AB  - The synthesis of SBA-15 material with spherical particles is performed by the template method by using only a surfactant Pluronic P123 under acidic conditions. In the synthesis of SBA-15 with spherical particles, an HCl solution was used after specialised chemical treatment of clay purification. The dominant presence of the spheres with diameters up to around 2 μm was confirmed by the scanning electron microscopy(SEM) method. In contrast, the Energy-dispersive X-ray spectroscopy(EDS) confirmed that the spheres consisted only of SiO2 in composition. In addition to the methods mentioned above, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy(FTIR) methods were used to characterise SBA- 15 materials. Application of HCl solution after chemical treatment of clay purification represents the application of technology in the synthesis of spherical SBA-15.
PB  - Belgrade : Serbian Ceramic Society
C3  - Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
T1  - Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant
SP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10799
ER  - 

@conference{
author = "Kokunešoski, Maja and Bašćarević, Zvezdana and Ilić, Svetlana and Valenta-Šobot, Ana and Grce, Ana and Pošarac-Marković, Milica B. and Šaponjić, Aleksandra",
year = "2021",
abstract = "The synthesis of SBA-15 material with spherical particles is performed by the template method by using only a surfactant Pluronic P123 under acidic conditions. In the synthesis of SBA-15 with spherical particles, an HCl solution was used after specialised chemical treatment of clay purification. The dominant presence of the spheres with diameters up to around 2 μm was confirmed by the scanning electron microscopy(SEM) method. In contrast, the Energy-dispersive X-ray spectroscopy(EDS) confirmed that the spheres consisted only of SiO2 in composition. In addition to the methods mentioned above, X-ray diffraction (XRD), and Fourier-transform infrared spectroscopy(FTIR) methods were used to characterise SBA- 15 materials. Application of HCl solution after chemical treatment of clay purification represents the application of technology in the synthesis of spherical SBA-15.",
publisher = "Belgrade : Serbian Ceramic Society",
journal = "Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade",
title = "Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant",
pages = "66",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10799"
}

Kokunešoski, M., Bašćarević, Z., Ilić, S., Valenta-Šobot, A., Grce, A., Pošarac-Marković, M. B.,& Šaponjić, A.. (2021). Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade
Belgrade : Serbian Ceramic Society., 66.
https://hdl.handle.net/21.15107/rcub_vinar_10799

Kokunešoski M, Bašćarević Z, Ilić S, Valenta-Šobot A, Grce A, Pošarac-Marković MB, Šaponjić A. Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant. in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade. 2021;:66.
https://hdl.handle.net/21.15107/rcub_vinar_10799 .

Kokunešoski, Maja, Bašćarević, Zvezdana, Ilić, Svetlana, Valenta-Šobot, Ana, Grce, Ana, Pošarac-Marković, Milica B., Šaponjić, Aleksandra, "Synthesis of spherical SBA-15 silica particles without the use of additional cosurfactant" in Advanced Ceramics and Application : 9th Serbian Ceramic Society Conference : program and the book of abstracts; September 20-21, 2021; Belgrade (2021):66,
https://hdl.handle.net/21.15107/rcub_vinar_10799 .

TY  - JOUR
AU  - Valenta-Šobot, Ana
AU  - Savić, Jasmina
AU  - Filipović Tričković, Jelena G.
AU  - Drakulić, Dunja R.
AU  - Joksić, Gordana
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10254
AB  - Root of Gentiana lutea commercially available as gentian root, a natural antidote for different types of poisons, possess antioxidative, immunomodulatory, cytoprotective and anti-inflammatory, and adverse, genotoxic and mutagenic effects. It has monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside as most abundant constituents. In this study, we assessed the toxicity of monoterpenes’ reactive molecular fragments using in silico prediction by VEGA-QSAR platform. Further, we compared the data obtained with in vitro geno- and cyto- toxicity testing of the above monoterpenes and the G. lutea root extract (GE), on human primary unstimulated and mitogen-stimulated peripheral blood mononuclear cells (PBMCs). Viability was assessed by TB and XTT tests after 48 h treatment. DNA damage was evaluated by alkaline comet assay on unstimulated cells, whereas cytokinesis-block micronucleus assay was employed on mitogen-stimulated PBMCs. Stability of compounds throughout treatment was monitored by UPLC. The observed in vitro results had highest compliance with in silico IRFMN/ISSCAN-CGX prediction model. Compounds showed high stability during experiment while treatment with single compounds reduced number of viable cells and increased DNA damage. GE treatment had toxic impact on unstimulated PBMCs but no significant genotoxic influence on mitogen-stimulated PBMCs. In summary, the mild GE effect suggests that the complexity of crude GE extract chemical composition  may attenuate the toxicity of the tested monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside.
T2  - Indian Journal of Experimental Biology
T1  - Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds
VL  - 58
IS  - 9
SP  - 609
EP  - 616
DO  - 10.56042/ijeb.v58i09.39874
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10254
ER  - 

@article{
author = "Valenta-Šobot, Ana and Savić, Jasmina and Filipović Tričković, Jelena G. and Drakulić, Dunja R. and Joksić, Gordana",
year = "2020",
abstract = "Root of Gentiana lutea commercially available as gentian root, a natural antidote for different types of poisons, possess antioxidative, immunomodulatory, cytoprotective and anti-inflammatory, and adverse, genotoxic and mutagenic effects. It has monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside as most abundant constituents. In this study, we assessed the toxicity of monoterpenes’ reactive molecular fragments using in silico prediction by VEGA-QSAR platform. Further, we compared the data obtained with in vitro geno- and cyto- toxicity testing of the above monoterpenes and the G. lutea root extract (GE), on human primary unstimulated and mitogen-stimulated peripheral blood mononuclear cells (PBMCs). Viability was assessed by TB and XTT tests after 48 h treatment. DNA damage was evaluated by alkaline comet assay on unstimulated cells, whereas cytokinesis-block micronucleus assay was employed on mitogen-stimulated PBMCs. Stability of compounds throughout treatment was monitored by UPLC. The observed in vitro results had highest compliance with in silico IRFMN/ISSCAN-CGX prediction model. Compounds showed high stability during experiment while treatment with single compounds reduced number of viable cells and increased DNA damage. GE treatment had toxic impact on unstimulated PBMCs but no significant genotoxic influence on mitogen-stimulated PBMCs. In summary, the mild GE effect suggests that the complexity of crude GE extract chemical composition  may attenuate the toxicity of the tested monoterpenes loganic acid, swertiamarin, gentiopicroside and sweroside.",
journal = "Indian Journal of Experimental Biology",
title = "Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds",
volume = "58",
number = "9",
pages = "609-616",
doi = "10.56042/ijeb.v58i09.39874",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10254"
}

Valenta-Šobot, A., Savić, J., Filipović Tričković, J. G., Drakulić, D. R.,& Joksić, G.. (2020). Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds. in Indian Journal of Experimental Biology, 58(9), 609-616.
https://doi.org/10.56042/ijeb.v58i09.39874
https://hdl.handle.net/21.15107/rcub_vinar_10254

Valenta-Šobot A, Savić J, Filipović Tričković JG, Drakulić DR, Joksić G. Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds. in Indian Journal of Experimental Biology. 2020;58(9):609-616.
doi:10.56042/ijeb.v58i09.39874
https://hdl.handle.net/21.15107/rcub_vinar_10254 .

Valenta-Šobot, Ana, Savić, Jasmina, Filipović Tričković, Jelena G., Drakulić, Dunja R., Joksić, Gordana, "Toxicity assessment of Gentiana lutea L. root extract and its monoterpene compounds" in Indian Journal of Experimental Biology, 58, no. 9 (2020):609-616,
https://doi.org/10.56042/ijeb.v58i09.39874 .,
https://hdl.handle.net/21.15107/rcub_vinar_10254 .