Preliminary cytotoxicity testing of newly synthesised SBA-15 material
Аутори
Kokunešoski, MajaŠaponjić, Aleksandra
Baščarević, Zvezdana
Jovanović, Ivan
Filipović Tričković, Jelena G.
Valenta-Šobot, Ana
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using
Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica.
The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al.
Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained
after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher
temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled
water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres
of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive
X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in compositio...n. In addition, X-ray diffraction and
Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic
effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from
particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated
with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical
damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium
had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.
Кључне речи:
Spherical SBA-15 / Pluronic P123 / CytotoxicityИзвор:
MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina, 2023, 60-Издавач:
- Association of Metallurgical Engineers of Serbia (AMES)
Колекције
Институција/група
VinčaTY - CONF AU - Kokunešoski, Maja AU - Šaponjić, Aleksandra AU - Baščarević, Zvezdana AU - Jovanović, Ivan AU - Filipović Tričković, Jelena G. AU - Valenta-Šobot, Ana PY - 2023 UR - https://vinar.vin.bg.ac.rs/handle/123456789/11280 AB - Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica. The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al. Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required. PB - Association of Metallurgical Engineers of Serbia (AMES) C3 - MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina T1 - Preliminary cytotoxicity testing of newly synthesised SBA-15 material SP - 60 UR - https://hdl.handle.net/21.15107/rcub_vinar_11280 ER -
@conference{ author = "Kokunešoski, Maja and Šaponjić, Aleksandra and Baščarević, Zvezdana and Jovanović, Ivan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana", year = "2023", abstract = "Material SBA-15 with all of its particles in the form of regular spheres was synthesised by template method using Pluronic P123 (non-ionic triblock copolymer) as a surfactant and tetraethoxysilane (TEOS, 98%) as a source of silica. The synthesis of spherical SBA-15 was carried out in acidic conditions according to method proposed by Zhao et al. Instead of expensive commercial cetyltrimethylammonium bromide as a co-surfactant, a spent HCl solution obtained after the chemical treatment of clay in an aqueous solution of HCl was used. The mixture was aged at a higher temperature. After that, the spherical SBA-15 was made by drying at room temperature after washing with distilled water and then calcining in flowing air to decompose the triblock copolymer. The continual presence of spheres of diameters around 2 μm in the entire SBA-15 was confirmed by scanning electron microscopy. The Energy-dispersive X-ray spectroscopy confirmed that the spheres consisted only of SiO2 in composition. In addition, X-ray diffraction and Fourier-transform infrared spectroscopy methods were used to characterise SBA-15 material. To assess the cytotoxic effects of newly synthesised SBA-15 material, HEK 293 cells were treated with particles and extracts obtained from particles incubated 24 hours at 37 °C in serum-free DMEM medium and DMEM with 10% serum. Cells were treated with concentrations of 100, 250, and 500 µg/mL for 72 hours. All treatments reduced cell viability in a concentrationdependent manner (p<0.001). Cytotoxicity was more pronounced in particle treatment, indicating that mechanical damage was induced in cells, whereas the extraction process influences the level of toxicity since the sera-free medium had the mildest effect. To acquire safe particles for human use, additional synthesis optimisation is required.", publisher = "Association of Metallurgical Engineers of Serbia (AMES)", journal = "MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina", title = "Preliminary cytotoxicity testing of newly synthesised SBA-15 material", pages = "60", url = "https://hdl.handle.net/21.15107/rcub_vinar_11280" }
Kokunešoski, M., Šaponjić, A., Baščarević, Z., Jovanović, I., Filipović Tričković, J. G.,& Valenta-Šobot, A.. (2023). Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina Association of Metallurgical Engineers of Serbia (AMES)., 60. https://hdl.handle.net/21.15107/rcub_vinar_11280
Kokunešoski M, Šaponjić A, Baščarević Z, Jovanović I, Filipović Tričković JG, Valenta-Šobot A. Preliminary cytotoxicity testing of newly synthesised SBA-15 material. in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina. 2023;:60. https://hdl.handle.net/21.15107/rcub_vinar_11280 .
Kokunešoski, Maja, Šaponjić, Aleksandra, Baščarević, Zvezdana, Jovanović, Ivan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, "Preliminary cytotoxicity testing of newly synthesised SBA-15 material" in MME SEE : 5th Metallurgical and Materials Engineering Congress of South-East Europe : book of abstracts; June 7-10, Trebinje, Bosnia and Herzegovina (2023):60, https://hdl.handle.net/21.15107/rcub_vinar_11280 .