Mesoporous silica decrease cell viability in vitro in dose dependent manner
Аутори
Valenta-Šobot, AnaFilipović Tričković, Jelena G.
Šaponjić, Aleksandra
Ilić, Svetlana
Grce, Ana
Katnić, Đurica
Kokunešoski, Maja
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Mesoporous silica renders chemical and mechanical stability under biological conditions, but due to its physicochemical properties, could be potentially harmful to exposed cells. The aim of the current study was to estimate SBA-15 (concentration of 50, 100 and 250 µg/mL) impact on human peripheral blood mononuclear cells to induce cyto- and genotoxicity in vitro after 72 h treatment, as well as lipid peroxidation in serum samples ex vivo. SBA-15 mesoporous silica treatment impact on cell viability was performed by XTT assay, lipid peroxidation was estimated by determining malondialdehyde and 4-hydroxyalkenals levels and genotoxicity by micronucleus assay. SBA-15 treatments displayed genotoxic potential at the lowest concentration, while highest tested concentration led to decrease of cell viability and increase of lipid peroxidation. Chemical modification of SBA-15 material that could influence its physicochemical properties could be useful way to lower its toxicity.
Извор:
PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry, 2021, II, 442-444Издавач:
- Society of Physical Chemists of Serbia
Финансирање / пројекти:
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
Колекције
Институција/група
VinčaTY - CONF AU - Valenta-Šobot, Ana AU - Filipović Tričković, Jelena G. AU - Šaponjić, Aleksandra AU - Ilić, Svetlana AU - Grce, Ana AU - Katnić, Đurica AU - Kokunešoski, Maja PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/11293 AB - Mesoporous silica renders chemical and mechanical stability under biological conditions, but due to its physicochemical properties, could be potentially harmful to exposed cells. The aim of the current study was to estimate SBA-15 (concentration of 50, 100 and 250 µg/mL) impact on human peripheral blood mononuclear cells to induce cyto- and genotoxicity in vitro after 72 h treatment, as well as lipid peroxidation in serum samples ex vivo. SBA-15 mesoporous silica treatment impact on cell viability was performed by XTT assay, lipid peroxidation was estimated by determining malondialdehyde and 4-hydroxyalkenals levels and genotoxicity by micronucleus assay. SBA-15 treatments displayed genotoxic potential at the lowest concentration, while highest tested concentration led to decrease of cell viability and increase of lipid peroxidation. Chemical modification of SBA-15 material that could influence its physicochemical properties could be useful way to lower its toxicity. PB - Society of Physical Chemists of Serbia C3 - PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry T1 - Mesoporous silica decrease cell viability in vitro in dose dependent manner VL - II SP - 442 EP - 444 UR - https://hdl.handle.net/21.15107/rcub_vinar_11293 ER -
@conference{ author = "Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Šaponjić, Aleksandra and Ilić, Svetlana and Grce, Ana and Katnić, Đurica and Kokunešoski, Maja", year = "2021", abstract = "Mesoporous silica renders chemical and mechanical stability under biological conditions, but due to its physicochemical properties, could be potentially harmful to exposed cells. The aim of the current study was to estimate SBA-15 (concentration of 50, 100 and 250 µg/mL) impact on human peripheral blood mononuclear cells to induce cyto- and genotoxicity in vitro after 72 h treatment, as well as lipid peroxidation in serum samples ex vivo. SBA-15 mesoporous silica treatment impact on cell viability was performed by XTT assay, lipid peroxidation was estimated by determining malondialdehyde and 4-hydroxyalkenals levels and genotoxicity by micronucleus assay. SBA-15 treatments displayed genotoxic potential at the lowest concentration, while highest tested concentration led to decrease of cell viability and increase of lipid peroxidation. Chemical modification of SBA-15 material that could influence its physicochemical properties could be useful way to lower its toxicity.", publisher = "Society of Physical Chemists of Serbia", journal = "PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry", title = "Mesoporous silica decrease cell viability in vitro in dose dependent manner", volume = "II", pages = "442-444", url = "https://hdl.handle.net/21.15107/rcub_vinar_11293" }
Valenta-Šobot, A., Filipović Tričković, J. G., Šaponjić, A., Ilić, S., Grce, A., Katnić, Đ.,& Kokunešoski, M.. (2021). Mesoporous silica decrease cell viability in vitro in dose dependent manner. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry Society of Physical Chemists of Serbia., II, 442-444. https://hdl.handle.net/21.15107/rcub_vinar_11293
Valenta-Šobot A, Filipović Tričković JG, Šaponjić A, Ilić S, Grce A, Katnić Đ, Kokunešoski M. Mesoporous silica decrease cell viability in vitro in dose dependent manner. in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry. 2021;II:442-444. https://hdl.handle.net/21.15107/rcub_vinar_11293 .
Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Šaponjić, Aleksandra, Ilić, Svetlana, Grce, Ana, Katnić, Đurica, Kokunešoski, Maja, "Mesoporous silica decrease cell viability in vitro in dose dependent manner" in PHYSICAL CHEMISTRY 2021 : 15th international conference on fundamental and applied aspects of physical chemistry, II (2021):442-444, https://hdl.handle.net/21.15107/rcub_vinar_11293 .