TY  - JOUR
AU  - Joksić, Gordana
AU  - Rutqvist, Lars Erik
AU  - Mićić, Mileva
AU  - Filipović Tričković, Jelena G.
AU  - Nilsson, Robert
PY  - 2019
UR  - https://linkinghub.elsevier.com/retrieve/pii/S0273230019300601
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8080
AB  - Long term exposure to oral smokeless tobacco may induce lesions in the oral cavity characterized by a hyperplastic epithelium. The possible role of nicotine and the physical properties of oral tobacco for developing these lesions, as well as of dysplasia and neoplasia is unclear. Low nitrosamine Swedish snus as well as non-genotoxic butylated hydroxyanisole induces increased cellular proliferation in the rat forestomach epithelia. Using this model, we report here on the effects of nicotine, pH, and particle size. Snus with different properties had no impact on oxidative stress as determined by 8-oxo-7,8-dihydro-2′-deoxyguanosine, or on interleukin IL-1b. Whereas BHA boosted IL-6, probably due to the presence of nicotine. there was no significant enhancement of cell divisions with increasing particle size, although in individual samples the variations in proliferation rates increased greatly with increasing particle size. Conforming to human experience, the enhanced cell proliferation caused by snus was found to be completely reversible. A cacao bean extract had a protective action similar to that previously found for blueberries. The main cause of the observed tobacco induced cell proliferation could be mechanical irritation, possibly in combination with nicotine, whereas within the studied range, pH did not affect the rate of cell division. © 2019
T2  - Regulatory Toxicology and Pharmacology
T1  - Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus)
VL  - 104
SP  - 21
EP  - 28
DO  - 10.1016/j.yrtph.2019.02.015
ER  - 

@article{
author = "Joksić, Gordana and Rutqvist, Lars Erik and Mićić, Mileva and Filipović Tričković, Jelena G. and Nilsson, Robert",
year = "2019",
abstract = "Long term exposure to oral smokeless tobacco may induce lesions in the oral cavity characterized by a hyperplastic epithelium. The possible role of nicotine and the physical properties of oral tobacco for developing these lesions, as well as of dysplasia and neoplasia is unclear. Low nitrosamine Swedish snus as well as non-genotoxic butylated hydroxyanisole induces increased cellular proliferation in the rat forestomach epithelia. Using this model, we report here on the effects of nicotine, pH, and particle size. Snus with different properties had no impact on oxidative stress as determined by 8-oxo-7,8-dihydro-2′-deoxyguanosine, or on interleukin IL-1b. Whereas BHA boosted IL-6, probably due to the presence of nicotine. there was no significant enhancement of cell divisions with increasing particle size, although in individual samples the variations in proliferation rates increased greatly with increasing particle size. Conforming to human experience, the enhanced cell proliferation caused by snus was found to be completely reversible. A cacao bean extract had a protective action similar to that previously found for blueberries. The main cause of the observed tobacco induced cell proliferation could be mechanical irritation, possibly in combination with nicotine, whereas within the studied range, pH did not affect the rate of cell division. © 2019",
journal = "Regulatory Toxicology and Pharmacology",
title = "Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus)",
volume = "104",
pages = "21-28",
doi = "10.1016/j.yrtph.2019.02.015"
}

Joksić, G., Rutqvist, L. E., Mićić, M., Filipović Tričković, J. G.,& Nilsson, R.. (2019). Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus). in Regulatory Toxicology and Pharmacology, 104, 21-28.
https://doi.org/10.1016/j.yrtph.2019.02.015

Joksić G, Rutqvist LE, Mićić M, Filipović Tričković JG, Nilsson R. Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus). in Regulatory Toxicology and Pharmacology. 2019;104:21-28.
doi:10.1016/j.yrtph.2019.02.015 .

Joksić, Gordana, Rutqvist, Lars Erik, Mićić, Mileva, Filipović Tričković, Jelena G., Nilsson, Robert, "Factors effecting the induction of rat forestomach hyperplasia induced by Swedish oral smokeless tobacco (snus)" in Regulatory Toxicology and Pharmacology, 104 (2019):21-28,
https://doi.org/10.1016/j.yrtph.2019.02.015 . .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Mićić, Mileva
AU  - Filipović, Jelena G.
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Calija, Bojan
AU  - Valenta-Šobot, Ana
AU  - Demajo, Miroslav
AU  - Nilsson, Robert
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1584
AB  - The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
T2  - Acta Veterinaria, Beograd
T1  - Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach
VL  - 67
IS  - 1
SP  - 1
EP  - 10
DO  - 10.1515/acve-2017-0001
ER  - 

@article{
author = "Joksić, Gordana and Mićić, Mileva and Filipović, Jelena G. and Drakulić, Dunja R. and Stanojlović, Miloš R. and Calija, Bojan and Valenta-Šobot, Ana and Demajo, Miroslav and Nilsson, Robert",
year = "2017",
abstract = "The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for subcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.",
journal = "Acta Veterinaria, Beograd",
title = "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach",
volume = "67",
number = "1",
pages = "1-10",
doi = "10.1515/acve-2017-0001"
}

Joksić, G., Mićić, M., Filipović, J. G., Drakulić, D. R., Stanojlović, M. R., Calija, B., Valenta-Šobot, A., Demajo, M.,& Nilsson, R.. (2017). Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd, 67(1), 1-10.
https://doi.org/10.1515/acve-2017-0001

Joksić G, Mićić M, Filipović JG, Drakulić DR, Stanojlović MR, Calija B, Valenta-Šobot A, Demajo M, Nilsson R. Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach. in Acta Veterinaria, Beograd. 2017;67(1):1-10.
doi:10.1515/acve-2017-0001 .

Joksić, Gordana, Mićić, Mileva, Filipović, Jelena G., Drakulić, Dunja R., Stanojlović, Miloš R., Calija, Bojan, Valenta-Šobot, Ana, Demajo, Miroslav, Nilsson, Robert, "Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach" in Acta Veterinaria, Beograd, 67, no. 1 (2017):1-10,
https://doi.org/10.1515/acve-2017-0001 . .

TY  - JOUR
AU  - Nilsson, Robert
AU  - Mićić, Mileva
AU  - Filipović, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Joksić, Gordana
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1058
AB  - The aim of this study was to identify palatable additives which have a significant protective action against soft tissue changes in the oral cavity caused by Swedish smokeless tobacco (snus), and that satisfy existing legal requirements. Although the cancer risk from snus is extremely low, long term use may result in highly undesirable keratotic lesions and associated epithelial abnormalities in the oral cavity. The rat forestomach, which is vulnerable to the irritative action of non-genotoxic compounds like butylated hydroxyanisole, propionic acid as well as snus, was chosen as an experimental model. Studied toxicological endpoints included histopathology and cellular proliferation based on DNA incorporation of bromodeoxyuridine. After 6 weeks exposure, blueberries (bilberries) and an extract from the common milk thistle were found to exert a highly significant inhibition of cell proliferation induced by snus in the rat forestomach epithelium, indicating a potential protection with respect soft tissue changes in the human oral cavity. (C) 2016 Elsevier Inc. All rights reserved.
PB  - Elsevier
T2  - Regulatory Toxicology and Pharmacology
T1  - Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus)
VL  - 76
SP  - 94
EP  - 101
DO  - 10.1016/j.yrtph.2016.01.017
ER  - 

@article{
author = "Nilsson, Robert and Mićić, Mileva and Filipović, Jelena G. and Valenta-Šobot, Ana and Drakulić, Dunja R. and Stanojlović, Miloš R. and Joksić, Gordana",
year = "2016",
abstract = "The aim of this study was to identify palatable additives which have a significant protective action against soft tissue changes in the oral cavity caused by Swedish smokeless tobacco (snus), and that satisfy existing legal requirements. Although the cancer risk from snus is extremely low, long term use may result in highly undesirable keratotic lesions and associated epithelial abnormalities in the oral cavity. The rat forestomach, which is vulnerable to the irritative action of non-genotoxic compounds like butylated hydroxyanisole, propionic acid as well as snus, was chosen as an experimental model. Studied toxicological endpoints included histopathology and cellular proliferation based on DNA incorporation of bromodeoxyuridine. After 6 weeks exposure, blueberries (bilberries) and an extract from the common milk thistle were found to exert a highly significant inhibition of cell proliferation induced by snus in the rat forestomach epithelium, indicating a potential protection with respect soft tissue changes in the human oral cavity. (C) 2016 Elsevier Inc. All rights reserved.",
publisher = "Elsevier",
journal = "Regulatory Toxicology and Pharmacology",
title = "Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus)",
volume = "76",
pages = "94-101",
doi = "10.1016/j.yrtph.2016.01.017"
}

Nilsson, R., Mićić, M., Filipović, J. G., Valenta-Šobot, A., Drakulić, D. R., Stanojlović, M. R.,& Joksić, G.. (2016). Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus). in Regulatory Toxicology and Pharmacology
Elsevier., 76, 94-101.
https://doi.org/10.1016/j.yrtph.2016.01.017

Nilsson R, Mićić M, Filipović JG, Valenta-Šobot A, Drakulić DR, Stanojlović MR, Joksić G. Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus). in Regulatory Toxicology and Pharmacology. 2016;76:94-101.
doi:10.1016/j.yrtph.2016.01.017 .

Nilsson, Robert, Mićić, Mileva, Filipović, Jelena G., Valenta-Šobot, Ana, Drakulić, Dunja R., Stanojlović, Miloš R., Joksić, Gordana, "Inhibition by blueberries (bilberries) and extract from milk thistle of rat forestomach hyperplasia induced by oral smokeless tobacco (Swedish snus)" in Regulatory Toxicology and Pharmacology, 76 (2016):94-101,
https://doi.org/10.1016/j.yrtph.2016.01.017 . .