Cell Proliferation Assay - Method Optimisation for in Vivo Labeling of DNA in the Rat Forestomach
Filipović, Jelena G.
Drakulić, Dunja R.
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The study of cell proliferation is a useful tool in the fields of toxicology, pathophysiology and pharmacology. Cell proliferation and its degree can be evaluated using 5-bromo-2deoxyuridine which is incorporated into the newly synthesized DNA. The aim of this study was the optimization of subcutaneous application of 5-bromo-2-deoxyuridine implantation for continuous and persistent marking of proliferating cells in the rat forestomach. 3-tert-Butyl-4-hydroxyanisole was used as the agent that ensures cell proliferation. In order to determine the optimal dose for proliferating cells labeling, 5-bromo-2-deoxyuridine doses of 50 mg, 100 mg, 200 mg or 350 mg were implemented 2 days prior to sacrifice by flat-faced cylindrical matrices. Immunohistochemical analysis using 5-bromo-2-deoxyuridine in situ detection kit was performed for the detection of 5-bromo-2-deoxyuridine labeled cells. The results showed that for adult rats, the optimum 5-bromo-2-deoxyuridine dose is 200 mg per animal for s...ubcutaneous application. The here described manner of 5-bromo-2-deoxyuridine in vivo labeling provides a simple, efficient, and reliable method for cell labeling, and at the same minimizes stress to animals.
Кључне речи:5-bromo-2 -deoxyuridine / 3-tert-Butyl-4-hydroxyanisole / BrdU in vivo labeling / cell proliferation / rat forestomach
Извор:Acta Veterinaria, Beograd, 2017, 67, 1, 1-10
- Радиосензитивност хуманог генома (RS-173046)
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