Background: Ferroptosis is one of the processes that could drive immune-mediated neurodegeneration in multiple sclerosis (MS). Exosomes as biologically active extracellular nanovesicles can carry miRNAs and are easily delivered across blood - brain barrier. Our aim is to provide data about the difference in network interplay of exosome carried miRNAs and PBMC mRNA between mild and severe MS phenotypes, in context of ferroptosis process regulation. Methods: Targeted mRNA sequencing of selected ferroptosis related genes was performed in PBMC of two MS phenotypes on Illumina iSeq100 NGS instrument. DESeq2 algorithm was used for obtaining DE genes and miRNA/mRNA interplay was woven using miRNET platform.
Keywords:
multiple sclerosis / mRNA / ferroptosis
Source:
Small New World 2.0, 2023, 108-108
Publisher:
Serbian Society for Extracellular Vesicles (SrbEVs)
Austrian Society for Extracellular Vesicles (ASEV)
Hungarian Section for Extracellular Vesicles (HSEV)
Slovenian Netvork for Extracellular Vesicles (SiN-EV)
TY - CONF
AU - Kuveljić, Jovana
AU - Jovanović, Ivan G.
AU - Kosanović, Maja
AU - Mačak, Nataša
AU - Đurić, Tamara
AU - Stanković, Aleksandra
AU - Živković, Maja
PY - 2023
UR - https://vinar.vin.bg.ac.rs/handle/123456789/14687
AB - Background: Ferroptosis is one of the processes that could drive immune-mediated neurodegeneration in multiple sclerosis (MS). Exosomes as biologically active extracellular nanovesicles can carry miRNAs and are easily delivered across blood - brain barrier. Our aim is to provide data about the difference in network interplay of exosome carried miRNAs and PBMC mRNA between mild and severe MS phenotypes, in context of ferroptosis process regulation. Methods: Targeted mRNA sequencing of selected ferroptosis related genes was performed in PBMC of two MS phenotypes on Illumina iSeq100 NGS instrument. DESeq2 algorithm was used for obtaining DE genes and miRNA/mRNA interplay was woven using miRNET platform.
PB - Serbian Society for Extracellular Vesicles (SrbEVs)
PB - Austrian Society for Extracellular Vesicles (ASEV)
PB - Hungarian Section for Extracellular Vesicles (HSEV)
PB - Slovenian Netvork for Extracellular Vesicles (SiN-EV)
C3 - Small New World 2.0
T1 - The crosstalk between circulating exosome carried miRNAs and ferroptosis related genes in multiple sclerosis
SP - 108
EP - 108
UR - https://hdl.handle.net/21.15107/rcub_vinar_14687
ER -
@conference{
author = "Kuveljić, Jovana and Jovanović, Ivan G. and Kosanović, Maja and Mačak, Nataša and Đurić, Tamara and Stanković, Aleksandra and Živković, Maja",
year = "2023",
abstract = "Background: Ferroptosis is one of the processes that could drive immune-mediated neurodegeneration in multiple sclerosis (MS). Exosomes as biologically active extracellular nanovesicles can carry miRNAs and are easily delivered across blood - brain barrier. Our aim is to provide data about the difference in network interplay of exosome carried miRNAs and PBMC mRNA between mild and severe MS phenotypes, in context of ferroptosis process regulation. Methods: Targeted mRNA sequencing of selected ferroptosis related genes was performed in PBMC of two MS phenotypes on Illumina iSeq100 NGS instrument. DESeq2 algorithm was used for obtaining DE genes and miRNA/mRNA interplay was woven using miRNET platform.",
publisher = "Serbian Society for Extracellular Vesicles (SrbEVs), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Section for Extracellular Vesicles (HSEV), Slovenian Netvork for Extracellular Vesicles (SiN-EV)",
journal = "Small New World 2.0",
title = "The crosstalk between circulating exosome carried miRNAs and ferroptosis related genes in multiple sclerosis",
pages = "108-108",
url = "https://hdl.handle.net/21.15107/rcub_vinar_14687"
}
Kuveljić, J., Jovanović, I. G., Kosanović, M., Mačak, N., Đurić, T., Stanković, A.,& Živković, M.. (2023). The crosstalk between circulating exosome carried miRNAs and ferroptosis related genes in multiple sclerosis. in Small New World 2.0
Serbian Society for Extracellular Vesicles (SrbEVs)., 108-108.
https://hdl.handle.net/21.15107/rcub_vinar_14687
Kuveljić J, Jovanović IG, Kosanović M, Mačak N, Đurić T, Stanković A, Živković M. The crosstalk between circulating exosome carried miRNAs and ferroptosis related genes in multiple sclerosis. in Small New World 2.0. 2023;:108-108.
https://hdl.handle.net/21.15107/rcub_vinar_14687 .
Kuveljić, Jovana, Jovanović, Ivan G., Kosanović, Maja, Mačak, Nataša, Đurić, Tamara, Stanković, Aleksandra, Živković, Maja, "The crosstalk between circulating exosome carried miRNAs and ferroptosis related genes in multiple sclerosis" in Small New World 2.0 (2023):108-108,
https://hdl.handle.net/21.15107/rcub_vinar_14687 .
