Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer
Само за регистроване кориснике
2018
Аутори
Eljabo, NajibNikolić, Nađa
Čarkić, Jelena
Jelovac, Drago B.
Lazarević, Miloš M.
Tanić, Nasta
Milašin, Jelena
Чланак у часопису (Објављена верзија)
,
© 2018 International Association of Oral and Maxillofacial Surgeons
Метаподаци
Приказ свих података о документуАпстракт
Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent t...han in unaffected oral mucosa (P < 0.001 and P = 0.027, respectively). HER2 amplification in margin tissue (P < 0.001) and swabs (P = 0.013), as well as the existence of three co-alterations in margins (P = 0.001) and macroscopically unaffected oral mucosa (P < 0.001) were correlated with shorter disease-specific survival.
Кључне речи:
gene amplification / promoter hypermethylation / OSCC / tumour margin / field cancerizationИзвор:
International Journal of Oral and Maxillofacial Surgery, 2018, 47, 8, 976-982Финансирање / пројекти:
- Генетичка контрола и молекуларни механизми у малигним, инфламаторним и развојним патологијама орофацијалне регије (RS-MESTD-Basic Research (BR or ON)-175075)
DOI: 10.1016/j.ijom.2018.01.020
ISSN: 0901-5027; 1399-0020
PubMed: 29449053
WoS: 000439537800005
Scopus: 2-s2.0-85044738507
URI
https://linkinghub.elsevier.com/retrieve/pii/S0901502718300389https://vinar.vin.bg.ac.rs/handle/123456789/7924
Институција/група
VinčaTY - JOUR AU - Eljabo, Najib AU - Nikolić, Nađa AU - Čarkić, Jelena AU - Jelovac, Drago B. AU - Lazarević, Miloš M. AU - Tanić, Nasta AU - Milašin, Jelena PY - 2018 UR - https://linkinghub.elsevier.com/retrieve/pii/S0901502718300389 UR - https://vinar.vin.bg.ac.rs/handle/123456789/7924 AB - Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent than in unaffected oral mucosa (P < 0.001 and P = 0.027, respectively). HER2 amplification in margin tissue (P < 0.001) and swabs (P = 0.013), as well as the existence of three co-alterations in margins (P = 0.001) and macroscopically unaffected oral mucosa (P < 0.001) were correlated with shorter disease-specific survival. T2 - International Journal of Oral and Maxillofacial Surgery T1 - Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer VL - 47 IS - 8 SP - 976 EP - 982 DO - 10.1016/j.ijom.2018.01.020 ER -
@article{ author = "Eljabo, Najib and Nikolić, Nađa and Čarkić, Jelena and Jelovac, Drago B. and Lazarević, Miloš M. and Tanić, Nasta and Milašin, Jelena", year = "2018", abstract = "Despite adequate surgical resection, oral squamous cell carcinoma (OSCC) shows a high rate of recurrence and metastasis, which could be explained by the presence of molecular alterations in seemingly normal tumour margins and the entire oral mucosa. The aims of this study were (1) to assess the presence of gene amplification (c-Myc and HER2) and promoter methylation (p14 and p16) in the tumours, tumour margins, and unaffected oral mucosa of 40 OSCC patients, and (2) to evaluate the possibility of using these alterations as prognostic markers. c-Myc and HER2 genes were quantified by means of real-time PCR (qPCR), and p14 and p16 methylation status was determined by methylation-specific PCR (MSP PCR). All tissues examined exhibited molecular alterations in various proportions. Tumour tissues, as expected, showed the highest prevalence of alterations, while oral mucosa showed the lowest. Multiple alterations (co-alterations) in tumours and tumour margins were significantly more frequent than in unaffected oral mucosa (P < 0.001 and P = 0.027, respectively). HER2 amplification in margin tissue (P < 0.001) and swabs (P = 0.013), as well as the existence of three co-alterations in margins (P = 0.001) and macroscopically unaffected oral mucosa (P < 0.001) were correlated with shorter disease-specific survival.", journal = "International Journal of Oral and Maxillofacial Surgery", title = "Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer", volume = "47", number = "8", pages = "976-982", doi = "10.1016/j.ijom.2018.01.020" }
Eljabo, N., Nikolić, N., Čarkić, J., Jelovac, D. B., Lazarević, M. M., Tanić, N.,& Milašin, J.. (2018). Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer. in International Journal of Oral and Maxillofacial Surgery, 47(8), 976-982. https://doi.org/10.1016/j.ijom.2018.01.020
Eljabo N, Nikolić N, Čarkić J, Jelovac DB, Lazarević MM, Tanić N, Milašin J. Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer. in International Journal of Oral and Maxillofacial Surgery. 2018;47(8):976-982. doi:10.1016/j.ijom.2018.01.020 .
Eljabo, Najib, Nikolić, Nađa, Čarkić, Jelena, Jelovac, Drago B., Lazarević, Miloš M., Tanić, Nasta, Milašin, Jelena, "Genetic and epigenetic alterations in the tumour, tumour margins, and normal buccal mucosa of patients with oral cancer" in International Journal of Oral and Maxillofacial Surgery, 47, no. 8 (2018):976-982, https://doi.org/10.1016/j.ijom.2018.01.020 . .