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Estradiol affect Na-dependent Ca2+ efflux from synaptosomal mitochondria
dc.creator | Horvat, Anica | |
dc.creator | Petrović, S | |
dc.creator | Nedeljković, N. | |
dc.creator | Martinović, Jelena | |
dc.creator | Nikezić, Gordana S. | |
dc.date.accessioned | 2018-03-01T18:51:43Z | |
dc.date.available | 2018-03-01T18:51:43Z | |
dc.date.issued | 2000 | |
dc.identifier.issn | 0231-5882 | |
dc.identifier.issn | 1338-4325 | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/2353 | |
dc.description.abstract | The effects of gonadal steroid hormone, 17 beta-estradiol (E-2), in vitro on rat brain mitochondria Ca2+ movement were investigated. Intrasynaptosomal mitochondria Ca2+ uptake via an energy-driven Ca2+ uniporter have K-m = 112.73 +/- 7.3 mu mol.l(-1) and V-max = 21.97 +/- 1.7 nmol Ca-45(2+) mg(-1). Ca2+ release trough a Na+/Ca2+ antiporter was measured with a K-m for Na+ of 43.7 +/- 2.6 mmol.l(-1), and V-max of 1.5 +/- 0.3 nmol Ca-45(2+) mg(-1). Addition of estradiol in preincubation mixture did not affect the uptake of Ca2+ mediated by the ruthenium red-sensitive uniporter, while it produced biphasic effect on Na-dependent Ca2+ efflux. Estradiol at concentrations up to 1 nmol.l(-1) decreased the efflux significantly (63% inhibition with respect to the control), and at concentrations above 10 nmol.l(-1) increased it exponentially. The maximum inhibiting concentration of estradiol (0.5 nmol.l(-1)) increased the affinity of the uniporter (K-m reduced by about 30%), without affecting significantly the capacity (V-max) for Na+. The results presented suggest that estradiol inhibits Na-dependent Ca2+ efflux from mitochondria and acts on mitochondrial retention of Ca2+, which may modulate mitochondrial and consequently synaptosomal content of Ca2+, and in this way exerts its role in the homeostasis of calcium in nerve terminals. | en |
dc.rights | restrictedAccess | en |
dc.source | General Physiology and Biophysics | en |
dc.subject | mitochondria | en |
dc.subject | Ca2+ transport | en |
dc.subject | estradiol | en |
dc.subject | synaptosomes | en |
dc.title | Estradiol affect Na-dependent Ca2+ efflux from synaptosomal mitochondria | en |
dc.type | article | en |
dcterms.abstract | Петровиц, С; Недељковиц, Н; Никезиц, Г; Хорват, Aница; Мартиновић Јелена; | |
dc.citation.volume | 19 | |
dc.citation.issue | 1 | |
dc.citation.spage | 59 | |
dc.citation.epage | 71 | |
dc.identifier.wos | 000087611800004 | |
dc.citation.rank | M23 | |
dc.identifier.pmid | 10930139 | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_vinar_2353 |
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