The crosstalk between circulating exosome carried miRNAs and ferroptosis related genes in multiple sclerosis

Abstract

Background: Ferroptosis is one of the processes that could drive immune-mediated neurodegeneration in multiple sclerosis (MS). Exosomes as biologically active extracellular nanovesicles can carry miRNAs and are easily delivered across blood - brain barrier. Our aim is to provide data about the difference in network interplay of exosome carried miRNAs and PBMC mRNA between mild and severe MS phenotypes, in context of ferroptosis process regulation. Methods: Targeted mRNA sequencing of selected ferroptosis related genes was performed in PBMC of two MS phenotypes on Illumina iSeq100 NGS instrument. DESeq2 algorithm was used for obtaining DE genes and miRNA/mRNA interplay was woven using miRNET platform.