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Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes
dc.creator | Stanković, Aleksandra | |
dc.creator | Jovanović, Ivan G. | |
dc.creator | Dinčić, E. | |
dc.creator | Vojinović, S. | |
dc.creator | Stojković, Ljiljana S. | |
dc.creator | Đorđević, Ana | |
dc.creator | Kuveljić, Jovana | |
dc.creator | Živković, Maja | |
dc.date.accessioned | 2024-01-31T12:03:43Z | |
dc.date.available | 2024-01-31T12:03:43Z | |
dc.date.issued | 2023 | |
dc.identifier.uri | https://web.archive.org/web/20240131090652/https://cony2023.comtecmed.com/e-posters/ | |
dc.identifier.uri | https://vinar.vin.bg.ac.rs/handle/123456789/12641 | |
dc.description.abstract | Molecular background and biomarkers of highly heterogenous and hardly predictable disease progression among relapse-onset MS patients are of high research interest. In the current pilot study, we aimed to employ next-generation sequencing to investigate the expression of whole small non-coding microRNAs (miRNome) in two groups of MS patients with highly distinctive progression phenotype: one with fast progressing, severely disabling course vs. mild course of MS, longitudinally followed 10 years. Peripheral blood mononuclear cells (PBMC) miRNome data was obtained from mild phenotype MS (n=4 patients) and progressive phenotype MS (n=5 patients), using TakaraBio SMARTer smRNA-Seq Kit on iSeq100 (Illumina). Pre-processing of raw sequencing data, quality control and miRNA differential expression analysis was performed using sRNAtoolbox pipeline. Functional interpretation of differentially expressed miRNA target genes was done in DIANA-miRPathv3.0. Tarbase v8.0 served as a resource of miRNA:gene interactions. Achieved read depth was approximately 1 million raw reads/sample, allowing detection of up to 92 mature miRNAs after genome alignment and miRbase v22 annotation. Differential expression analysis identified the significant upregulation of hsa-miR-23c (log2FC=4.29, Padj= 0.03) in progressive phenotype. Top significantly enriched KEGG pathways in hsa-miR-23c targets suggested regulation of molecular pathways involved in autoimmunity (antigen presentation, Epstein-Barr virus infection) and cancer. In conclusion, this pilot study indicates phenotype-related differences in expression of miRNAs, molecules with high regulatory and biomarker properties. Although detected in PBMC, has-miR-23c is highly expressed in the brain and target MS relevant genes such as, HLA (A, B, C), transferrin receptor, Nrf2, recently proposed to play important role in neurodegeneration. | en |
dc.language.iso | en | |
dc.rights | openAccess | |
dc.source | 17th World Congress on Controversies in Neurology (CONy) : e-posters | |
dc.title | Insight in miRNome of severe multiple sclerosis: Pilot study of distinctive relapse-onset MS phenotypes | en |
dc.type | conferenceObject | |
dc.rights.license | ARR | |
dc.citation.spage | 427 | |
dc.citation.epage | 427 | |
dc.type.version | publishedVersion | |
dc.identifier.fulltext | http://vinar.vin.bg.ac.rs/bitstream/id/34774/427.pdf | |
dc.identifier.rcub | https://hdl.handle.net/21.15107/rcub_vinar_12641 |
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