TY  - JOUR
AU  - Vučić, Vesna
AU  - Isenović, Esma R.
AU  - Adžić, Miroslav
AU  - Ruždijić, Sabera
AU  - Radojčić, Marija
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2977
AB  - Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of Co-60 gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P LT 0.001) antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control) to 49% (IR cells), with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.
T2  - Brazilian Journal of Medical and Biological Research
T1  - Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells
VL  - 39
IS  - 2
SP  - 227
EP  - 236
DO  - 10.1590/S0100-879X2006000200009
ER  - 

@article{
author = "Vučić, Vesna and Isenović, Esma R. and Adžić, Miroslav and Ruždijić, Sabera and Radojčić, Marija",
year = "2006",
abstract = "Gamma-irradiation (gamma-IR) is extensively used in the treatment of hormone-resistant prostate carcinoma. The objective of the present study was to investigate the effects of Co-60 gamma-IR on the growth, cell cycle arrest and cell death of the human prostate cancer cell line DU 145. The viability of DU 145 cells was measured by the Trypan blue exclusion assay and the 3(4,5-dimethylthiazol-2-yl)-2,5,diphenyltetrazolium bromide test. Bromodeoxyuridine incorporation was used for the determination of cell proliferation. Cell cycle arrest and cell death were analyzed by flow cytometry. Superoxide dismutase (SOD), specifically CuZnSOD and MnSOD protein expression, after 10 Gy gamma-IR, was determined by Western immunoblotting analysis. gamma-IR treatment had a significant (P LT 0.001) antiproliferative and cytotoxic effect on DU 145 cells. Both effects were time and dose dependent. Also, the dose of gamma-IR which inhibited DNA synthesis and cell proliferation by 50% was 9.7 Gy. Furthermore, gamma-IR induced cell cycle arrest in the G2/M phase and the percentage of cells in the G2/M phase was increased from 15% (control) to 49% (IR cells), with a nonsignificant induction of apoptosis. Treatment with 10 Gy gamma-IR for 24, 48, and 72 h stimulated CuZnSOD and MnSOD protein expression in a time-dependent manner, approximately by 3- to 3.5-fold. These data suggest that CuZnSOD and MnSOD enzymes may play an important role in the gamma-IR-induced changes in DU 145 cell growth, cell cycle arrest and cell death.",
journal = "Brazilian Journal of Medical and Biological Research",
title = "Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells",
volume = "39",
number = "2",
pages = "227-236",
doi = "10.1590/S0100-879X2006000200009"
}

Vučić, V., Isenović, E. R., Adžić, M., Ruždijić, S.,& Radojčić, M.. (2006). Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells. in Brazilian Journal of Medical and Biological Research, 39(2), 227-236.
https://doi.org/10.1590/S0100-879X2006000200009

Vučić V, Isenović ER, Adžić M, Ruždijić S, Radojčić M. Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells. in Brazilian Journal of Medical and Biological Research. 2006;39(2):227-236.
doi:10.1590/S0100-879X2006000200009 .

Vučić, Vesna, Isenović, Esma R., Adžić, Miroslav, Ruždijić, Sabera, Radojčić, Marija, "Effects of gamma-radiation on cell growth, cycle arrest, death, and superoxide dismutase expression by DU145 human prostate cancer cells" in Brazilian Journal of Medical and Biological Research, 39, no. 2 (2006):227-236,
https://doi.org/10.1590/S0100-879X2006000200009 . .