TY  - JOUR
AU  - Zmejkoski, Danica
AU  - Zdravković, Nemanja M.
AU  - Budimir Filimonović, Milica D.
AU  - Pavlović, Vladimir B.
AU  - Butulija, Svetlana
AU  - Milivojević, Dušan
AU  - Marković, Zoran M.
AU  - Todorović Marković, Biljana M.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12990
AB  - In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections.
T2  - Journal of Functional Biomaterials
T1  - Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light
VL  - 15
IS  - 3
SP  - 72
DO  - 10.3390/jfb15030072
ER  - 

@article{
author = "Zmejkoski, Danica and Zdravković, Nemanja M. and Budimir Filimonović, Milica D. and Pavlović, Vladimir B. and Butulija, Svetlana and Milivojević, Dušan and Marković, Zoran M. and Todorović Marković, Biljana M.",
year = "2024",
abstract = "In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections.",
journal = "Journal of Functional Biomaterials",
title = "Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light",
volume = "15",
number = "3",
pages = "72",
doi = "10.3390/jfb15030072"
}

Zmejkoski, D., Zdravković, N. M., Budimir Filimonović, M. D., Pavlović, V. B., Butulija, S., Milivojević, D., Marković, Z. M.,& Todorović Marković, B. M.. (2024). Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light. in Journal of Functional Biomaterials, 15(3), 72.
https://doi.org/10.3390/jfb15030072

Zmejkoski D, Zdravković NM, Budimir Filimonović MD, Pavlović VB, Butulija S, Milivojević D, Marković ZM, Todorović Marković BM. Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light. in Journal of Functional Biomaterials. 2024;15(3):72.
doi:10.3390/jfb15030072 .

Zmejkoski, Danica, Zdravković, Nemanja M., Budimir Filimonović, Milica D., Pavlović, Vladimir B., Butulija, Svetlana, Milivojević, Dušan, Marković, Zoran M., Todorović Marković, Biljana M., "Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light" in Journal of Functional Biomaterials, 15, no. 3 (2024):72,
https://doi.org/10.3390/jfb15030072 . .

TY  - JOUR
AU  - Marković, Zoran M.
AU  - Budimir Filimonović, Milica
AU  - Milivojević, Dušan
AU  - Kovač, Janez
AU  - Todorović-Marković, Biljana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12997
AB  - The cost of treatment of antibiotic-resistant pathogens is on the level of tens of billions of dollars at the moment. It is of special interest to reduce or solve this problem using antimicrobial coatings, especially in hospitals or other healthcare facilities. The bacteria can transfer from medical staff or contaminated surfaces to patients. In this paper, we focused our attention on the antibacterial and antibiofouling activities of two types of photodynamic polyurethane composite films doped with carbon polymerized dots (CPDs) and fullerene C60. Detailed atomic force, electrostatic force and viscoelastic microscopy revealed topology, nanoelectrical and nanomechanical properties of used fillers and composites. A relationship between the electronic structure of the nanocarbon fillers and the antibacterial and antibiofouling activities of the composites was established. Thorough spectroscopic analysis of reactive oxygen species (ROS) generation was conducted for both composite films, and it was found that both of them were potent antibacterial agents against nosocomial bacteria (Klebsiela pneumoniae, Proteus mirabilis, Salmonela enterica, Enterococcus faecalis, Enterococcus epidermis and Pseudomonas aeruginosa). Antibiofouling testing of composite films indicated that the CPDs/PU composite films eradicated almost completely the biofilms of Pseudomonas aeruginosa and Staphylococcus aureus and about 50% of Escherichia coli biofilms.
T2  - Journal of Functional Biomaterials
T1  - Antibacterial and Antibiofouling Activities of Carbon Polymerized Dots/Polyurethane and C60/Polyurethane Composite Films
VL  - 15
IS  - 3
SP  - 73
DO  - 10.3390/jfb15030073
ER  - 

@article{
author = "Marković, Zoran M. and Budimir Filimonović, Milica and Milivojević, Dušan and Kovač, Janez and Todorović-Marković, Biljana",
year = "2024",
abstract = "The cost of treatment of antibiotic-resistant pathogens is on the level of tens of billions of dollars at the moment. It is of special interest to reduce or solve this problem using antimicrobial coatings, especially in hospitals or other healthcare facilities. The bacteria can transfer from medical staff or contaminated surfaces to patients. In this paper, we focused our attention on the antibacterial and antibiofouling activities of two types of photodynamic polyurethane composite films doped with carbon polymerized dots (CPDs) and fullerene C60. Detailed atomic force, electrostatic force and viscoelastic microscopy revealed topology, nanoelectrical and nanomechanical properties of used fillers and composites. A relationship between the electronic structure of the nanocarbon fillers and the antibacterial and antibiofouling activities of the composites was established. Thorough spectroscopic analysis of reactive oxygen species (ROS) generation was conducted for both composite films, and it was found that both of them were potent antibacterial agents against nosocomial bacteria (Klebsiela pneumoniae, Proteus mirabilis, Salmonela enterica, Enterococcus faecalis, Enterococcus epidermis and Pseudomonas aeruginosa). Antibiofouling testing of composite films indicated that the CPDs/PU composite films eradicated almost completely the biofilms of Pseudomonas aeruginosa and Staphylococcus aureus and about 50% of Escherichia coli biofilms.",
journal = "Journal of Functional Biomaterials",
title = "Antibacterial and Antibiofouling Activities of Carbon Polymerized Dots/Polyurethane and C60/Polyurethane Composite Films",
volume = "15",
number = "3",
pages = "73",
doi = "10.3390/jfb15030073"
}

Marković, Z. M., Budimir Filimonović, M., Milivojević, D., Kovač, J.,& Todorović-Marković, B.. (2024). Antibacterial and Antibiofouling Activities of Carbon Polymerized Dots/Polyurethane and C60/Polyurethane Composite Films. in Journal of Functional Biomaterials, 15(3), 73.
https://doi.org/10.3390/jfb15030073

Marković ZM, Budimir Filimonović M, Milivojević D, Kovač J, Todorović-Marković B. Antibacterial and Antibiofouling Activities of Carbon Polymerized Dots/Polyurethane and C60/Polyurethane Composite Films. in Journal of Functional Biomaterials. 2024;15(3):73.
doi:10.3390/jfb15030073 .

Marković, Zoran M., Budimir Filimonović, Milica, Milivojević, Dušan, Kovač, Janez, Todorović-Marković, Biljana, "Antibacterial and Antibiofouling Activities of Carbon Polymerized Dots/Polyurethane and C60/Polyurethane Composite Films" in Journal of Functional Biomaterials, 15, no. 3 (2024):73,
https://doi.org/10.3390/jfb15030073 . .