Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light
Аутори
Zmejkoski, DanicaZdravković, Nemanja M.
Budimir Filimonović, Milica D.
Pavlović, Vladimir B.
Butulija, Svetlana
Milivojević, Dušan
Marković, Zoran M.
Todorović Marković, Biljana M.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combatin...g biofilm-related infections.
Кључне речи:
chitosan nanoparticles / bacterial cellulose / photoactive therapy / antibiofilm / nanocomposite hydrogels / blue light and green lightИзвор:
Journal of Functional Biomaterials, 2024, 15, 3, 72-Финансирање / пројекти:
- PHOTOGUN4MICROBES - Are photoactive nanoparticles salvation for global infectional treath? (RS-ScienceFundRS-Ideje-7741955)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200017 (Универзитет у Београду, Институт за нуклеарне науке Винча, Београд-Винча) (RS-MESTD-inst-2020-200017)
- Министарство науке, технолошког развоја и иновација Републике Србије, институционално финансирање - 200030 (Научни институт за ветеринарство Србије, Београд) (RS-MESTD-inst-2020-200030)
Колекције
Институција/група
VinčaTY - JOUR AU - Zmejkoski, Danica AU - Zdravković, Nemanja M. AU - Budimir Filimonović, Milica D. AU - Pavlović, Vladimir B. AU - Butulija, Svetlana AU - Milivojević, Dušan AU - Marković, Zoran M. AU - Todorović Marković, Biljana M. PY - 2024 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12990 AB - In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections. T2 - Journal of Functional Biomaterials T1 - Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light VL - 15 IS - 3 SP - 72 DO - 10.3390/jfb15030072 ER -
@article{ author = "Zmejkoski, Danica and Zdravković, Nemanja M. and Budimir Filimonović, Milica D. and Pavlović, Vladimir B. and Butulija, Svetlana and Milivojević, Dušan and Marković, Zoran M. and Todorović Marković, Biljana M.", year = "2024", abstract = "In this study, nanochitosan dots (ChiDs) were synthesized using gamma rays and encapsulated in bacterial cellulose (BC) polymer matrix for antibiofilm potential in photodynamic therapy. The composites were analyzed for structural changes using SEM, AFM, FTIR, XRD, EPR, and porosity measurements. Additionally, ChiD release was assessed. The results showed that the chemical composition remained unaltered, but ChiD agglomerates embedded in BC changed shape (1.5–2.5 µm). Bacterial cellulose fibers became deformed and interconnected, with increased surface roughness and porosity and decreased crystallinity. No singlet oxygen formation was observed, and the total amount of released ChiD was up to 16.10%. Antibiofilm activity was higher under green light, with reductions ranging from 48 to 57% under blue light and 78 to 85% under green light. Methicillin-resistant Staphylococcus aureus was the most sensitive strain. The new photoactive composite hydrogels show promising potential for combating biofilm-related infections.", journal = "Journal of Functional Biomaterials", title = "Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light", volume = "15", number = "3", pages = "72", doi = "10.3390/jfb15030072" }
Zmejkoski, D., Zdravković, N. M., Budimir Filimonović, M. D., Pavlović, V. B., Butulija, S., Milivojević, D., Marković, Z. M.,& Todorović Marković, B. M.. (2024). Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light. in Journal of Functional Biomaterials, 15(3), 72. https://doi.org/10.3390/jfb15030072
Zmejkoski D, Zdravković NM, Budimir Filimonović MD, Pavlović VB, Butulija S, Milivojević D, Marković ZM, Todorović Marković BM. Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light. in Journal of Functional Biomaterials. 2024;15(3):72. doi:10.3390/jfb15030072 .
Zmejkoski, Danica, Zdravković, Nemanja M., Budimir Filimonović, Milica D., Pavlović, Vladimir B., Butulija, Svetlana, Milivojević, Dušan, Marković, Zoran M., Todorović Marković, Biljana M., "Reduction in Pathogenic Biofilms by the Photoactive Composite of Bacterial Cellulose and Nanochitosan Dots under Blue and Green Light" in Journal of Functional Biomaterials, 15, no. 3 (2024):72, https://doi.org/10.3390/jfb15030072 . .