@conference{
author = "Horvat, Anica and Milošević, Maja and Petrović, Snježana and Demajo, Miroslav",
year = "2004",
abstract = "Ions of zinc and cooper represents micro elements present in all organisms but they can be accumulated in various tissues from the environment by food or pollution. In this work we examined the effects of chloride salts of Cu2+ and Zn2+ on the activity of ecto-ATPase, integral plasma membrane protein, in brain and ovarian cells. Both ions exhibit similar effects on brain or ovarian enzyme activity. Cooper in brain and ovary totally inhibits enzyme activity at the concentration of 1 mM and 0.1 M respectively. IC50 for brain is 36 µM while in ovary it is 192 µM. Zinc inhibits in both tissues 50% of the control enzyme activity at concentrations of 1 mM in brain and 0.1 M in ovary with IC50 of 1mM and 14 mM respectively. These metals possess an affinity for -SH groups in the enzyme protein, may replace Mg2+ in enzyme substrate, MgATP or to bind for enzyme substrate site. According to the total inhibition of ecto-ATPase activity by Cu 2+, it may be proposed that its site of action is on the substrate or substrate binding site, while Zn2+ modulates enzyme activity by acting on the –SH or S-S groups of the enzyme. Inhibiting or decreasing ecto-ATPase activity in brain and ovary, these two metal ions may be toxic and seriously disturb the proper functioning of the investigated tissues.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+",
volume = "1",
pages = "374-376",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9499"
}