Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+
Конференцијски прилог (Објављена верзија)
,
Society of Physical Chemists of Serbia
Метаподаци
Приказ свих података о документуАпстракт
Ions of zinc and cooper represents micro elements present in all organisms but they can be accumulated in various tissues from the environment by food or pollution. In this work we examined the effects of chloride salts of Cu2+ and Zn2+ on the activity of ecto-ATPase, integral plasma membrane protein, in brain and ovarian cells. Both ions exhibit similar effects on brain or ovarian enzyme activity. Cooper in brain and ovary totally inhibits enzyme activity at the concentration of 1 mM and 0.1 M respectively. IC50 for brain is 36 µM while in ovary it is 192 µM. Zinc inhibits in both tissues 50% of the control enzyme activity at concentrations of 1 mM in brain and 0.1 M in ovary with IC50 of 1mM and 14 mM respectively. These metals possess an affinity for -SH groups in the enzyme protein, may replace Mg2+ in enzyme substrate, MgATP or to bind for enzyme substrate site. According to the total inhibition of ecto-ATPase activity by Cu 2+, it may be proposed that its site of action is on the... substrate or substrate binding site, while Zn2+ modulates enzyme activity by acting on the –SH or S-S groups of the enzyme. Inhibiting or decreasing ecto-ATPase activity in brain and ovary, these two metal ions may be toxic and seriously disturb the proper functioning of the investigated tissues.
Извор:
Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 2004, 1, 374-376Издавач:
- Society of Physical Chemists of Serbia
Финансирање / пројекти:
- [1956]
Напомена:
- Physical chemistry 2004 : 7th international conference on fundamental and applied aspects of physical chemistry; Belgrade (Serbia); 21-23 September 2004
Колекције
Институција/група
VinčaTY - CONF AU - Horvat, Anica AU - Milošević, Maja AU - Petrović, Snježana AU - Demajo, Miroslav PY - 2004 UR - https://vinar.vin.bg.ac.rs/handle/123456789/9499 AB - Ions of zinc and cooper represents micro elements present in all organisms but they can be accumulated in various tissues from the environment by food or pollution. In this work we examined the effects of chloride salts of Cu2+ and Zn2+ on the activity of ecto-ATPase, integral plasma membrane protein, in brain and ovarian cells. Both ions exhibit similar effects on brain or ovarian enzyme activity. Cooper in brain and ovary totally inhibits enzyme activity at the concentration of 1 mM and 0.1 M respectively. IC50 for brain is 36 µM while in ovary it is 192 µM. Zinc inhibits in both tissues 50% of the control enzyme activity at concentrations of 1 mM in brain and 0.1 M in ovary with IC50 of 1mM and 14 mM respectively. These metals possess an affinity for -SH groups in the enzyme protein, may replace Mg2+ in enzyme substrate, MgATP or to bind for enzyme substrate site. According to the total inhibition of ecto-ATPase activity by Cu 2+, it may be proposed that its site of action is on the substrate or substrate binding site, while Zn2+ modulates enzyme activity by acting on the –SH or S-S groups of the enzyme. Inhibiting or decreasing ecto-ATPase activity in brain and ovary, these two metal ions may be toxic and seriously disturb the proper functioning of the investigated tissues. PB - Society of Physical Chemists of Serbia C3 - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry T1 - Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+ VL - 1 SP - 374 EP - 376 UR - https://hdl.handle.net/21.15107/rcub_vinar_9499 ER -
@conference{ author = "Horvat, Anica and Milošević, Maja and Petrović, Snježana and Demajo, Miroslav", year = "2004", abstract = "Ions of zinc and cooper represents micro elements present in all organisms but they can be accumulated in various tissues from the environment by food or pollution. In this work we examined the effects of chloride salts of Cu2+ and Zn2+ on the activity of ecto-ATPase, integral plasma membrane protein, in brain and ovarian cells. Both ions exhibit similar effects on brain or ovarian enzyme activity. Cooper in brain and ovary totally inhibits enzyme activity at the concentration of 1 mM and 0.1 M respectively. IC50 for brain is 36 µM while in ovary it is 192 µM. Zinc inhibits in both tissues 50% of the control enzyme activity at concentrations of 1 mM in brain and 0.1 M in ovary with IC50 of 1mM and 14 mM respectively. These metals possess an affinity for -SH groups in the enzyme protein, may replace Mg2+ in enzyme substrate, MgATP or to bind for enzyme substrate site. According to the total inhibition of ecto-ATPase activity by Cu 2+, it may be proposed that its site of action is on the substrate or substrate binding site, while Zn2+ modulates enzyme activity by acting on the –SH or S-S groups of the enzyme. Inhibiting or decreasing ecto-ATPase activity in brain and ovary, these two metal ions may be toxic and seriously disturb the proper functioning of the investigated tissues.", publisher = "Society of Physical Chemists of Serbia", journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry", title = "Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+", volume = "1", pages = "374-376", url = "https://hdl.handle.net/21.15107/rcub_vinar_9499" }
Horvat, A., Milošević, M., Petrović, S.,& Demajo, M.. (2004). Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry Society of Physical Chemists of Serbia., 1, 374-376. https://hdl.handle.net/21.15107/rcub_vinar_9499
Horvat A, Milošević M, Petrović S, Demajo M. Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:374-376. https://hdl.handle.net/21.15107/rcub_vinar_9499 .
Horvat, Anica, Milošević, Maja, Petrović, Snježana, Demajo, Miroslav, "Different sensibility of ecto-ATPase from brain and ovary to Cu2+ and Zn2+" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):374-376, https://hdl.handle.net/21.15107/rcub_vinar_9499 .