TY  - JOUR
AU  - Pavlović, Miloš
AU  - Đukić, Mirjana
AU  - Vojvodić, Danilo
AU  - Ninković, Milica
AU  - Stevanović, Ivana
AU  - Đurić, Ana
AU  - Stanojević, Boban
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9915
AB  - Background/Aim. Exposure of living organisms to γ-radiation results in the overproduction of free radicals. The aim of the study was to test if the subacute administration of micronized zeolite (MZC) accomplishes radioprotective role based on the evaluation of the status of oxidative stress (OS) in the brain and 8-hydroxyguanosine (8-OH-dG) in the plasma of the rats exposed to the single γ-ray irradiation of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a four-week normal or 5% MZC supplemented diet and afterward exposed to the single γ-ray irradiation of 2 and 10 Gy. Groups of rats were: a) on a normal diet (the control group, and 2Gy and 10Gy groups); b) on 5% MZC supplemented diet (the control group – MZC, MZC + 2Gy, and MZC + 10Gy groups). We measured malondialdehyde (MDA), glutathione (GSH) total, and activity of total super-oxide dismutase (tSOD) and manganese superoxide dismutase (MnSOD) in vulnerable brain regions (cerebellum, hippocampus, and cerebral cortex) and 8-OH-dG in plasma. Results. Lower MDA was found in the MZC+2Gy and MZC+10Gy groups compared to the 2Gy and 10Gy groups. Activity od total SOD was higher in the MZC+10Gy group than in the 10Gy one. GSH was the highest in the 10Gy group. Compared to the control group, 8-OH-dG was extremely higher in groups radiated with 10 Gy regardless of a diet, but slightly lower in the MZC+2Gy and 2Gy groups. Conclusion. Subacute MZC pretreatment accomplished partial radioprotective effect in irradiated rats compared to non-irradiated rats, based on suppressed SOD activity at 2 Gy, and reduced brain MDA when exposed to 2 Gy and 10 Gy.
AB  - Uvod/Cilj. Izlaganje živih organizama gama zračenju rezultira hiperprodukcijom slobodnih radikala. Cilj istraživanja je bio da se ispita da li subakutna ishrana dopunjena sa 5% mikronizovanog zeolita (MZC) ispoljava radiozaštitnu ulogu na osnovu statusa oksidativnog stresa (OS) u mozgu i 8-hidroksiguanozina (8-OH-dG) u plazmi pacova izloženih pojedinačnim dozama jonizujućeg zračenja od 2 i 10 Gray (Gy). Metode. Wistar pacovi su bili na četvoronedeljnoj normalnoj ishrani ili ishrani obogaćenoj sa 5% MZC, posle čega su bili izloženi pojedinačnom jonizujućem zračenju od 2 Gy, odnosno 10 Gy. Grupe pacova bile su: a) grupa pacova na normalnoj ishrani (kontrolna grupa i grupe 2Gy i 10Gy); b) grupa pacova na ishrani obogaćenoj sa 5% MZC (kontrolna grupa – MZC i grupe MZC+2Gy i MZC+10Gy). Meren je malondialdehid (MDA), glutation (GSH) i aktivnost ukupne (tSOD) i mangan superoksid dizmutaze (MnSOD) u osetljivim strukturama mozga (cerebelum, hipokampus i cerebralni korteks), a 8-OH-dG u plazmi. Rezultati. Biomarker MDA je bio niži u MZC+2Gy i MZC+10Gy grupama, u odnosu na grupe 2Gy i 10Gy. Aktivnost ukupne SOD je bila viša u grupi MZC+10Gy, u odnosu na grupu 10Gy. Najviši nivo GSH bio je u grupi 10Gy. U pređenju sa kontrolnom grupom, 8-OH-dG je bio izuzetno viši u grupama ozračenim sa 10 Gy, bez obzira na dijetetski režim i niži u grupama MZC+2Gy i 2Gy. Zaključak. Pacovi koji su bili na režimu ishrane obogaćene sa 5% MZC bili su delimično zaštićeni od zračenja, shodno redukovanoj moždanoj aktivnosti SOD pri 2 Gy i sniženom nivou MDA pri izlaganju zračenju od 2 i 10 Gy.
T2  - Vojnosanitetski pregled
T1  - Moderate radioprotective role of zeolite in rats
T1  - Umerena radioprotektivna uloga zeolita kod pacova
VL  - 78
IS  - 7
SP  - 760
EP  - 768
DO  - 10.2298/VSP190702136P
ER  - 

@article{
author = "Pavlović, Miloš and Đukić, Mirjana and Vojvodić, Danilo and Ninković, Milica and Stevanović, Ivana and Đurić, Ana and Stanojević, Boban",
year = "2021",
abstract = "Background/Aim. Exposure of living organisms to γ-radiation results in the overproduction of free radicals. The aim of the study was to test if the subacute administration of micronized zeolite (MZC) accomplishes radioprotective role based on the evaluation of the status of oxidative stress (OS) in the brain and 8-hydroxyguanosine (8-OH-dG) in the plasma of the rats exposed to the single γ-ray irradiation of 2 and/or 10 Gray (Gy). Methods. Wistar rats were on a four-week normal or 5% MZC supplemented diet and afterward exposed to the single γ-ray irradiation of 2 and 10 Gy. Groups of rats were: a) on a normal diet (the control group, and 2Gy and 10Gy groups); b) on 5% MZC supplemented diet (the control group – MZC, MZC + 2Gy, and MZC + 10Gy groups). We measured malondialdehyde (MDA), glutathione (GSH) total, and activity of total super-oxide dismutase (tSOD) and manganese superoxide dismutase (MnSOD) in vulnerable brain regions (cerebellum, hippocampus, and cerebral cortex) and 8-OH-dG in plasma. Results. Lower MDA was found in the MZC+2Gy and MZC+10Gy groups compared to the 2Gy and 10Gy groups. Activity od total SOD was higher in the MZC+10Gy group than in the 10Gy one. GSH was the highest in the 10Gy group. Compared to the control group, 8-OH-dG was extremely higher in groups radiated with 10 Gy regardless of a diet, but slightly lower in the MZC+2Gy and 2Gy groups. Conclusion. Subacute MZC pretreatment accomplished partial radioprotective effect in irradiated rats compared to non-irradiated rats, based on suppressed SOD activity at 2 Gy, and reduced brain MDA when exposed to 2 Gy and 10 Gy., Uvod/Cilj. Izlaganje živih organizama gama zračenju rezultira hiperprodukcijom slobodnih radikala. Cilj istraživanja je bio da se ispita da li subakutna ishrana dopunjena sa 5% mikronizovanog zeolita (MZC) ispoljava radiozaštitnu ulogu na osnovu statusa oksidativnog stresa (OS) u mozgu i 8-hidroksiguanozina (8-OH-dG) u plazmi pacova izloženih pojedinačnim dozama jonizujućeg zračenja od 2 i 10 Gray (Gy). Metode. Wistar pacovi su bili na četvoronedeljnoj normalnoj ishrani ili ishrani obogaćenoj sa 5% MZC, posle čega su bili izloženi pojedinačnom jonizujućem zračenju od 2 Gy, odnosno 10 Gy. Grupe pacova bile su: a) grupa pacova na normalnoj ishrani (kontrolna grupa i grupe 2Gy i 10Gy); b) grupa pacova na ishrani obogaćenoj sa 5% MZC (kontrolna grupa – MZC i grupe MZC+2Gy i MZC+10Gy). Meren je malondialdehid (MDA), glutation (GSH) i aktivnost ukupne (tSOD) i mangan superoksid dizmutaze (MnSOD) u osetljivim strukturama mozga (cerebelum, hipokampus i cerebralni korteks), a 8-OH-dG u plazmi. Rezultati. Biomarker MDA je bio niži u MZC+2Gy i MZC+10Gy grupama, u odnosu na grupe 2Gy i 10Gy. Aktivnost ukupne SOD je bila viša u grupi MZC+10Gy, u odnosu na grupu 10Gy. Najviši nivo GSH bio je u grupi 10Gy. U pređenju sa kontrolnom grupom, 8-OH-dG je bio izuzetno viši u grupama ozračenim sa 10 Gy, bez obzira na dijetetski režim i niži u grupama MZC+2Gy i 2Gy. Zaključak. Pacovi koji su bili na režimu ishrane obogaćene sa 5% MZC bili su delimično zaštićeni od zračenja, shodno redukovanoj moždanoj aktivnosti SOD pri 2 Gy i sniženom nivou MDA pri izlaganju zračenju od 2 i 10 Gy.",
journal = "Vojnosanitetski pregled",
title = "Moderate radioprotective role of zeolite in rats, Umerena radioprotektivna uloga zeolita kod pacova",
volume = "78",
number = "7",
pages = "760-768",
doi = "10.2298/VSP190702136P"
}

Pavlović, M., Đukić, M., Vojvodić, D., Ninković, M., Stevanović, I., Đurić, A.,& Stanojević, B.. (2021). Moderate radioprotective role of zeolite in rats. in Vojnosanitetski pregled, 78(7), 760-768.
https://doi.org/10.2298/VSP190702136P

Pavlović M, Đukić M, Vojvodić D, Ninković M, Stevanović I, Đurić A, Stanojević B. Moderate radioprotective role of zeolite in rats. in Vojnosanitetski pregled. 2021;78(7):760-768.
doi:10.2298/VSP190702136P .

Pavlović, Miloš, Đukić, Mirjana, Vojvodić, Danilo, Ninković, Milica, Stevanović, Ivana, Đurić, Ana, Stanojević, Boban, "Moderate radioprotective role of zeolite in rats" in Vojnosanitetski pregled, 78, no. 7 (2021):760-768,
https://doi.org/10.2298/VSP190702136P . .

TY  - JOUR
AU  - Nikolić, Milena D.
AU  - Pavlović, Aleksandra N.
AU  - Mitić, Snežana S.
AU  - Tošić, Snežana B.
AU  - Mitić, Milan N.
AU  - Kaličanin, Biljana M.
AU  - Manojlović, Dragan D.
AU  - Stanković, Dalibor M.
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8428
AB  - Background of the study – Among fruits, berry fruits contain a very large complex of chemical compounds that are responsible for their antioxidant properties. As far as we know there is no data available from cyclic voltammetry studies of berry fruits. Objectives – The first objective of the present work was to investigate the electrochemical properties of antioxidants present in four types of berry fruits: strawberries, blackberries, blueberries and red raspberries. The second objective was to establish the correlation between antioxidant capacities obtained from cyclic voltammograms with those obtained using spectrophotometric techniques. Methods – Evaluation of antioxidant activity of 15 berry samples (strawberry, blackberry, blueberry and raspberry) has been carried out using cyclic voltammetry (CV). CVs were taken in the potential range between 0 mV and 800 mV with a scan rate of 100 mV s-1. Results – A pronounced anodic current peak observed between 0.524 V and 0.540 V in all analyzed berry samples can be ascribed to anthocyanins, which are the major contributors to the antioxidant capacity of investigated berries. Results obtained from CVs were compared with those obtained by using DPPH, ABTS, FRAP and RP assays. ACI parameters were determined for each berry sample by assigning all the assays an equal weight. According to obtained results, the highest ACI had blueberry samples, followed by blackberry, raspberry and strawberry samples. Also, high positive correlations were found between antioxidant capacities deduced from CVs with those obtained using FRAP (R2 = 0.8630, p = 0.0006), RP (R2 = 0.7426, p = 0.0004), DPPH (R2 = 0.7680, p = 0.0001) and ABTS (R2 = 0.8710, p = 0.000002). In addition, high positive correlations were also found between total phenols, flavonoids, anthocyanins and CV antioxidant capacity (R2 = 0.8091, p = 0.0005; R2 = 0.8341, p = 0.0002; R2 = 0.7163, p = 0.0007), respectively. Conclusion – Obtained results showed that the cyclic voltammetry is a suitable method for the determination of the reducing capacity based on the electrochemical properties of compounds under the test. © ISHS 2019.
T2  - European Journal of Horticultural Science
T1  - Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays
VL  - 84
IS  - 3
SP  - 152
EP  - 160
DO  - 10.17660/eJHS.2019/84.3.5
ER  - 

@article{
author = "Nikolić, Milena D. and Pavlović, Aleksandra N. and Mitić, Snežana S. and Tošić, Snežana B. and Mitić, Milan N. and Kaličanin, Biljana M. and Manojlović, Dragan D. and Stanković, Dalibor M.",
year = "2019",
abstract = "Background of the study – Among fruits, berry fruits contain a very large complex of chemical compounds that are responsible for their antioxidant properties. As far as we know there is no data available from cyclic voltammetry studies of berry fruits. Objectives – The first objective of the present work was to investigate the electrochemical properties of antioxidants present in four types of berry fruits: strawberries, blackberries, blueberries and red raspberries. The second objective was to establish the correlation between antioxidant capacities obtained from cyclic voltammograms with those obtained using spectrophotometric techniques. Methods – Evaluation of antioxidant activity of 15 berry samples (strawberry, blackberry, blueberry and raspberry) has been carried out using cyclic voltammetry (CV). CVs were taken in the potential range between 0 mV and 800 mV with a scan rate of 100 mV s-1. Results – A pronounced anodic current peak observed between 0.524 V and 0.540 V in all analyzed berry samples can be ascribed to anthocyanins, which are the major contributors to the antioxidant capacity of investigated berries. Results obtained from CVs were compared with those obtained by using DPPH, ABTS, FRAP and RP assays. ACI parameters were determined for each berry sample by assigning all the assays an equal weight. According to obtained results, the highest ACI had blueberry samples, followed by blackberry, raspberry and strawberry samples. Also, high positive correlations were found between antioxidant capacities deduced from CVs with those obtained using FRAP (R2 = 0.8630, p = 0.0006), RP (R2 = 0.7426, p = 0.0004), DPPH (R2 = 0.7680, p = 0.0001) and ABTS (R2 = 0.8710, p = 0.000002). In addition, high positive correlations were also found between total phenols, flavonoids, anthocyanins and CV antioxidant capacity (R2 = 0.8091, p = 0.0005; R2 = 0.8341, p = 0.0002; R2 = 0.7163, p = 0.0007), respectively. Conclusion – Obtained results showed that the cyclic voltammetry is a suitable method for the determination of the reducing capacity based on the electrochemical properties of compounds under the test. © ISHS 2019.",
journal = "European Journal of Horticultural Science",
title = "Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays",
volume = "84",
number = "3",
pages = "152-160",
doi = "10.17660/eJHS.2019/84.3.5"
}

Nikolić, M. D., Pavlović, A. N., Mitić, S. S., Tošić, S. B., Mitić, M. N., Kaličanin, B. M., Manojlović, D. D.,& Stanković, D. M.. (2019). Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays. in European Journal of Horticultural Science, 84(3), 152-160.
https://doi.org/10.17660/eJHS.2019/84.3.5

Nikolić MD, Pavlović AN, Mitić SS, Tošić SB, Mitić MN, Kaličanin BM, Manojlović DD, Stanković DM. Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays. in European Journal of Horticultural Science. 2019;84(3):152-160.
doi:10.17660/eJHS.2019/84.3.5 .

Nikolić, Milena D., Pavlović, Aleksandra N., Mitić, Snežana S., Tošić, Snežana B., Mitić, Milan N., Kaličanin, Biljana M., Manojlović, Dragan D., Stanković, Dalibor M., "Use of cyclic voltammetry to determine the antioxidant capacity of berry fruits: correlation with spectrophotometric assays" in European Journal of Horticultural Science, 84, no. 3 (2019):152-160,
https://doi.org/10.17660/eJHS.2019/84.3.5 . .

TY  - JOUR
AU  - Stanojević, Boban
AU  - Đukić, Mirjana
AU  - Stevanović, Ivana
AU  - Ninković, Milica
AU  - Đurić, Ana
AU  - Gobeljić, Borko
AU  - Apostolović, Milan
AU  - Pantelić, Ana
AU  - Zebić, Goran
AU  - Todorović, Lidija
AU  - Bojić, Tijana
AU  - Savovski, Kiril
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/8863
AB  - The aim of our study was to test the effect of subacutely applied micronized zeolite [micronized clinoptilolite (MZC)] on brain status of iron (Fe), reactive oxygen and nitrogen species (ROS, RNS), and radioprotective role against brain oxidative/nitrosative stress (OS/NS) initiated by single ionizing radiation of 2 or 10Gray (Gy). Wistar rats on normal (n=18) and 5% MZC supplemented diet (n=18), during 4 weeks, were internally subdivided into 3 subgroups (6 rats in each subgroup), with one of subgroup remaining as a control, and the other two subjected to single ionizing radiation of 2Gy or 10Gy. Thus, we had groups on normal diet: C – controls, 2Gy and 10Gy; and on 5% MZC supplemented diet: MZC, MZC+2Gy and MZC+10Gy. Concentrations of nitrates (a final RNS metabolite) and superoxide anion radical (O2 •-) (an initial ROS) were measured in homogenates of selective vulnerable brain regions (cerebellum, hippocampus and forebrain cortex), while Fe was determined in whole brain of rats. Results documented a significant drop of Fe in MZC and MZC+2Gy/10Gy groups; decrease of O2 •- and nitrate in MZC group; almost equal drop of O2 •- , in 2Gy and MZC+2Gy groups; and nitrate increase in 10Gy and MZC+10Gy groups. We confirmed that subacute MZC pretreatment contributes to partially accomplished brain radioprotective effect in rats exposed to single radiation dose of 2Gy and 10Gy, probably due to reduced OS/NS and Fe.
T2  - Hrana i ishrana
T1  - Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats
VL  - 59
IS  - 2
SP  - 26
EP  - 32
DO  - 10.5937/hraish1801026s
ER  - 

@article{
author = "Stanojević, Boban and Đukić, Mirjana and Stevanović, Ivana and Ninković, Milica and Đurić, Ana and Gobeljić, Borko and Apostolović, Milan and Pantelić, Ana and Zebić, Goran and Todorović, Lidija and Bojić, Tijana and Savovski, Kiril",
year = "2018",
abstract = "The aim of our study was to test the effect of subacutely applied micronized zeolite [micronized clinoptilolite (MZC)] on brain status of iron (Fe), reactive oxygen and nitrogen species (ROS, RNS), and radioprotective role against brain oxidative/nitrosative stress (OS/NS) initiated by single ionizing radiation of 2 or 10Gray (Gy). Wistar rats on normal (n=18) and 5% MZC supplemented diet (n=18), during 4 weeks, were internally subdivided into 3 subgroups (6 rats in each subgroup), with one of subgroup remaining as a control, and the other two subjected to single ionizing radiation of 2Gy or 10Gy. Thus, we had groups on normal diet: C – controls, 2Gy and 10Gy; and on 5% MZC supplemented diet: MZC, MZC+2Gy and MZC+10Gy. Concentrations of nitrates (a final RNS metabolite) and superoxide anion radical (O2 •-) (an initial ROS) were measured in homogenates of selective vulnerable brain regions (cerebellum, hippocampus and forebrain cortex), while Fe was determined in whole brain of rats. Results documented a significant drop of Fe in MZC and MZC+2Gy/10Gy groups; decrease of O2 •- and nitrate in MZC group; almost equal drop of O2 •- , in 2Gy and MZC+2Gy groups; and nitrate increase in 10Gy and MZC+10Gy groups. We confirmed that subacute MZC pretreatment contributes to partially accomplished brain radioprotective effect in rats exposed to single radiation dose of 2Gy and 10Gy, probably due to reduced OS/NS and Fe.",
journal = "Hrana i ishrana",
title = "Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats",
volume = "59",
number = "2",
pages = "26-32",
doi = "10.5937/hraish1801026s"
}

Stanojević, B., Đukić, M., Stevanović, I., Ninković, M., Đurić, A., Gobeljić, B., Apostolović, M., Pantelić, A., Zebić, G., Todorović, L., Bojić, T.,& Savovski, K.. (2018). Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats. in Hrana i ishrana, 59(2), 26-32.
https://doi.org/10.5937/hraish1801026s

Stanojević B, Đukić M, Stevanović I, Ninković M, Đurić A, Gobeljić B, Apostolović M, Pantelić A, Zebić G, Todorović L, Bojić T, Savovski K. Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats. in Hrana i ishrana. 2018;59(2):26-32.
doi:10.5937/hraish1801026s .

Stanojević, Boban, Đukić, Mirjana, Stevanović, Ivana, Ninković, Milica, Đurić, Ana, Gobeljić, Borko, Apostolović, Milan, Pantelić, Ana, Zebić, Goran, Todorović, Lidija, Bojić, Tijana, Savovski, Kiril, "Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats" in Hrana i ishrana, 59, no. 2 (2018):26-32,
https://doi.org/10.5937/hraish1801026s . .