Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats
2018
Преузимање 🢃
Аутори
Stanojević, BobanĐukić, Mirjana
Stevanović, Ivana
Ninković, Milica
Đurić, Ana
Gobeljić, Borko
Apostolović, Milan
Pantelić, Ana
Zebić, Goran
Todorović, Lidija
Bojić, Tijana
Savovski, Kiril
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
The aim of our study was to test the effect of subacutely applied micronized zeolite [micronized clinoptilolite (MZC)] on brain status of iron (Fe), reactive oxygen and nitrogen species (ROS, RNS), and radioprotective role against brain oxidative/nitrosative stress (OS/NS) initiated by single ionizing radiation of 2 or 10Gray (Gy). Wistar rats on normal (n=18) and 5% MZC supplemented diet (n=18), during 4 weeks, were internally subdivided into 3 subgroups (6 rats in each subgroup), with one of subgroup remaining as a control, and the other two subjected to single ionizing radiation of 2Gy or 10Gy. Thus, we had groups on normal diet: C – controls, 2Gy and 10Gy; and on 5% MZC supplemented diet: MZC, MZC+2Gy and MZC+10Gy. Concentrations of nitrates (a final RNS metabolite) and superoxide anion radical (O2 •-) (an initial ROS) were measured in homogenates of selective vulnerable brain regions (cerebellum, hippocampus and forebrain cortex), while Fe was determined in whole brain of rats. Re...sults documented a significant drop of Fe in MZC and MZC+2Gy/10Gy groups; decrease of O2 •- and nitrate in MZC group; almost equal drop of O2 •- , in 2Gy and MZC+2Gy groups; and nitrate increase in 10Gy and MZC+10Gy groups. We confirmed that subacute MZC pretreatment contributes to partially accomplished brain radioprotective effect in rats exposed to single radiation dose of 2Gy and 10Gy, probably due to reduced OS/NS and Fe.
Кључне речи:
brain / iron / ionizing radiation / nitrosative stress / oxidative stress / zeolite (MZC)Извор:
Hrana i ishrana, 2018, 59, 2, 26-32Финансирање / пројекти:
- Превентивни, терапијски и етички приступ преклиничким и клиничким истраживањима гена и модулатора редокс ћелијске сигнализације у имунском, инфламаторном и пролиферативном одговору ћелије (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-41018)
- Ministry of Defense of the Republic of Serbia [MFVМА/6/15–17]
- Ministry of Defense of the Republic of Serbia [MFVМА/04/16–18]
Колекције
Институција/група
VinčaTY - JOUR AU - Stanojević, Boban AU - Đukić, Mirjana AU - Stevanović, Ivana AU - Ninković, Milica AU - Đurić, Ana AU - Gobeljić, Borko AU - Apostolović, Milan AU - Pantelić, Ana AU - Zebić, Goran AU - Todorović, Lidija AU - Bojić, Tijana AU - Savovski, Kiril PY - 2018 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8863 AB - The aim of our study was to test the effect of subacutely applied micronized zeolite [micronized clinoptilolite (MZC)] on brain status of iron (Fe), reactive oxygen and nitrogen species (ROS, RNS), and radioprotective role against brain oxidative/nitrosative stress (OS/NS) initiated by single ionizing radiation of 2 or 10Gray (Gy). Wistar rats on normal (n=18) and 5% MZC supplemented diet (n=18), during 4 weeks, were internally subdivided into 3 subgroups (6 rats in each subgroup), with one of subgroup remaining as a control, and the other two subjected to single ionizing radiation of 2Gy or 10Gy. Thus, we had groups on normal diet: C – controls, 2Gy and 10Gy; and on 5% MZC supplemented diet: MZC, MZC+2Gy and MZC+10Gy. Concentrations of nitrates (a final RNS metabolite) and superoxide anion radical (O2 •-) (an initial ROS) were measured in homogenates of selective vulnerable brain regions (cerebellum, hippocampus and forebrain cortex), while Fe was determined in whole brain of rats. Results documented a significant drop of Fe in MZC and MZC+2Gy/10Gy groups; decrease of O2 •- and nitrate in MZC group; almost equal drop of O2 •- , in 2Gy and MZC+2Gy groups; and nitrate increase in 10Gy and MZC+10Gy groups. We confirmed that subacute MZC pretreatment contributes to partially accomplished brain radioprotective effect in rats exposed to single radiation dose of 2Gy and 10Gy, probably due to reduced OS/NS and Fe. T2 - Hrana i ishrana T1 - Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats VL - 59 IS - 2 SP - 26 EP - 32 DO - 10.5937/hraish1801026s ER -
@article{ author = "Stanojević, Boban and Đukić, Mirjana and Stevanović, Ivana and Ninković, Milica and Đurić, Ana and Gobeljić, Borko and Apostolović, Milan and Pantelić, Ana and Zebić, Goran and Todorović, Lidija and Bojić, Tijana and Savovski, Kiril", year = "2018", abstract = "The aim of our study was to test the effect of subacutely applied micronized zeolite [micronized clinoptilolite (MZC)] on brain status of iron (Fe), reactive oxygen and nitrogen species (ROS, RNS), and radioprotective role against brain oxidative/nitrosative stress (OS/NS) initiated by single ionizing radiation of 2 or 10Gray (Gy). Wistar rats on normal (n=18) and 5% MZC supplemented diet (n=18), during 4 weeks, were internally subdivided into 3 subgroups (6 rats in each subgroup), with one of subgroup remaining as a control, and the other two subjected to single ionizing radiation of 2Gy or 10Gy. Thus, we had groups on normal diet: C – controls, 2Gy and 10Gy; and on 5% MZC supplemented diet: MZC, MZC+2Gy and MZC+10Gy. Concentrations of nitrates (a final RNS metabolite) and superoxide anion radical (O2 •-) (an initial ROS) were measured in homogenates of selective vulnerable brain regions (cerebellum, hippocampus and forebrain cortex), while Fe was determined in whole brain of rats. Results documented a significant drop of Fe in MZC and MZC+2Gy/10Gy groups; decrease of O2 •- and nitrate in MZC group; almost equal drop of O2 •- , in 2Gy and MZC+2Gy groups; and nitrate increase in 10Gy and MZC+10Gy groups. We confirmed that subacute MZC pretreatment contributes to partially accomplished brain radioprotective effect in rats exposed to single radiation dose of 2Gy and 10Gy, probably due to reduced OS/NS and Fe.", journal = "Hrana i ishrana", title = "Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats", volume = "59", number = "2", pages = "26-32", doi = "10.5937/hraish1801026s" }
Stanojević, B., Đukić, M., Stevanović, I., Ninković, M., Đurić, A., Gobeljić, B., Apostolović, M., Pantelić, A., Zebić, G., Todorović, L., Bojić, T.,& Savovski, K.. (2018). Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats. in Hrana i ishrana, 59(2), 26-32. https://doi.org/10.5937/hraish1801026s
Stanojević B, Đukić M, Stevanović I, Ninković M, Đurić A, Gobeljić B, Apostolović M, Pantelić A, Zebić G, Todorović L, Bojić T, Savovski K. Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats. in Hrana i ishrana. 2018;59(2):26-32. doi:10.5937/hraish1801026s .
Stanojević, Boban, Đukić, Mirjana, Stevanović, Ivana, Ninković, Milica, Đurić, Ana, Gobeljić, Borko, Apostolović, Milan, Pantelić, Ana, Zebić, Goran, Todorović, Lidija, Bojić, Tijana, Savovski, Kiril, "Zeolite pretreatment accomplishes partial brain radioprotective role by reducing iron and oxidative / nitrosative stress in rats" in Hrana i ishrana, 59, no. 2 (2018):26-32, https://doi.org/10.5937/hraish1801026s . .