TY  - JOUR
AU  - Janković, Teodora
AU  - Savikin, Katarina
AU  - Menkovic, Nebojsa
AU  - Aljancic, Ivana
AU  - Leskovac, Andreja
AU  - Petrović, Sandra
AU  - Joksić, Gordana
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3482
AB  - The aim of this study was to identify active principles of Gentianella austriaca responsible for the reduction of the incidence of micronuclei in irradiated lymphocytes in vitro. The radioprotective effects of ether (EF) and methanolic (MeF) fractions, water-soluble xanthones demethylbellidifolin (1), demethylbellidifolin 8-O-glucoside (2), bellidifolin 8-O-glucoside (3), and flavonoid swertisin (4) against chromosomal damage induced by gamma-rays were determined using the micronucleus test. EF and MeF showed better protection in treatment of human lymphocytes after gamma-irradiation than did isolated compounds. Among the isolated compounds, the effectivenessin reduction of the frequency of micronuclei followed the order 4 GT 3 GT 2 GT 1. The anti-lipoperoxidant activity was in the order 2 GT 4 GT 1, while 3 slightly increased the level of malondialdehyde. In addition, the effectiveness in induction of apoptosis followed the order, 3 GT 2 GT 4, while 1 had no proapoptotic effect. These results suggest that the antioxidative properties of the polyphenols tested may contribute to the radioprotective effects of G. austriaca.
T2  - Planta Medica
T1  - Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes
VL  - 74
IS  - 7
SP  - 736
EP  - 740
DO  - 10.1055/s-2008-1074524
ER  - 

@article{
author = "Janković, Teodora and Savikin, Katarina and Menkovic, Nebojsa and Aljancic, Ivana and Leskovac, Andreja and Petrović, Sandra and Joksić, Gordana",
year = "2008",
abstract = "The aim of this study was to identify active principles of Gentianella austriaca responsible for the reduction of the incidence of micronuclei in irradiated lymphocytes in vitro. The radioprotective effects of ether (EF) and methanolic (MeF) fractions, water-soluble xanthones demethylbellidifolin (1), demethylbellidifolin 8-O-glucoside (2), bellidifolin 8-O-glucoside (3), and flavonoid swertisin (4) against chromosomal damage induced by gamma-rays were determined using the micronucleus test. EF and MeF showed better protection in treatment of human lymphocytes after gamma-irradiation than did isolated compounds. Among the isolated compounds, the effectivenessin reduction of the frequency of micronuclei followed the order 4 GT 3 GT 2 GT 1. The anti-lipoperoxidant activity was in the order 2 GT 4 GT 1, while 3 slightly increased the level of malondialdehyde. In addition, the effectiveness in induction of apoptosis followed the order, 3 GT 2 GT 4, while 1 had no proapoptotic effect. These results suggest that the antioxidative properties of the polyphenols tested may contribute to the radioprotective effects of G. austriaca.",
journal = "Planta Medica",
title = "Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes",
volume = "74",
number = "7",
pages = "736-740",
doi = "10.1055/s-2008-1074524"
}

Janković, T., Savikin, K., Menkovic, N., Aljancic, I., Leskovac, A., Petrović, S.,& Joksić, G.. (2008). Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes. in Planta Medica, 74(7), 736-740.
https://doi.org/10.1055/s-2008-1074524

Janković T, Savikin K, Menkovic N, Aljancic I, Leskovac A, Petrović S, Joksić G. Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes. in Planta Medica. 2008;74(7):736-740.
doi:10.1055/s-2008-1074524 .

Janković, Teodora, Savikin, Katarina, Menkovic, Nebojsa, Aljancic, Ivana, Leskovac, Andreja, Petrović, Sandra, Joksić, Gordana, "Radioprotective effects of Gentianella austriaca fractions and polyphenolic constituents in human lymphocytes" in Planta Medica, 74, no. 7 (2008):736-740,
https://doi.org/10.1055/s-2008-1074524 . .

TY  - JOUR
AU  - Leskovac, Andreja
AU  - Joksić, Gordana
AU  - Janković, Teodora
AU  - Savildn, Katarina
AU  - Menkovic, Nebojsa
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3302
AB  - The objective of the present study is to evaluate the radioprotective properties of extracts of Crataegus monogyna Jacq. (Rosaceae) fruit, Cornus mas L. (Cornaceae) leaves and Gentianella austriaca (A. Kern. and Jos. Kern.) Holub (Gentianaceae) aerial parts on cultured human peripheral blood lymphocytes in vitro after irradiation with 2 Gy of Co-60 gamma-rays. Plants were collected at Mt. Maljen in Serbia, air-dried and powdered, and the total phenolic content was analyzed. In C. mas leaves, ellagic and gallic acid were found to be the dominant compounds, whereas C. monogyna fruit was rich in procyanidins and flavonoids. The main constituents of G. austriaca aerial parts were gamma-pyrones and secoiridoids. Cell cultures were treated with four different doses of plant extracts (0.1 mg/mL to 0.4 mg/mL). Treatment with the lowest dose gave the best protective effect. Significant radiorecovery potentials of C. mas and C. monogyna were observed, seen as a reduced incidence of radiation-induced micronuclei (19.23 % and 13.24 %, respectively), reduced levels of lipid peroxidation products (50.04% and 13.18%, respectively) and two-fold enhanced apoptosis. Both extracts slowed down cell proliferation gradually, enabling more time for repair. G. austriaca possesses strong antioxidant properties, significantly reducing lipid peroxidation and the incidence of micronuclei (for 30.88 % and 35.56 %, respectively) while enhancing apoptosis with no perturbation of the cell cycle. This study may contribute to the search for novel radioprotective agents.
T2  - Planta Medica
T1  - Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro
VL  - 73
IS  - 11
SP  - 1169
EP  - 1175
DO  - 10.1055/s-2007-981586
ER  - 

@article{
author = "Leskovac, Andreja and Joksić, Gordana and Janković, Teodora and Savildn, Katarina and Menkovic, Nebojsa",
year = "2007",
abstract = "The objective of the present study is to evaluate the radioprotective properties of extracts of Crataegus monogyna Jacq. (Rosaceae) fruit, Cornus mas L. (Cornaceae) leaves and Gentianella austriaca (A. Kern. and Jos. Kern.) Holub (Gentianaceae) aerial parts on cultured human peripheral blood lymphocytes in vitro after irradiation with 2 Gy of Co-60 gamma-rays. Plants were collected at Mt. Maljen in Serbia, air-dried and powdered, and the total phenolic content was analyzed. In C. mas leaves, ellagic and gallic acid were found to be the dominant compounds, whereas C. monogyna fruit was rich in procyanidins and flavonoids. The main constituents of G. austriaca aerial parts were gamma-pyrones and secoiridoids. Cell cultures were treated with four different doses of plant extracts (0.1 mg/mL to 0.4 mg/mL). Treatment with the lowest dose gave the best protective effect. Significant radiorecovery potentials of C. mas and C. monogyna were observed, seen as a reduced incidence of radiation-induced micronuclei (19.23 % and 13.24 %, respectively), reduced levels of lipid peroxidation products (50.04% and 13.18%, respectively) and two-fold enhanced apoptosis. Both extracts slowed down cell proliferation gradually, enabling more time for repair. G. austriaca possesses strong antioxidant properties, significantly reducing lipid peroxidation and the incidence of micronuclei (for 30.88 % and 35.56 %, respectively) while enhancing apoptosis with no perturbation of the cell cycle. This study may contribute to the search for novel radioprotective agents.",
journal = "Planta Medica",
title = "Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro",
volume = "73",
number = "11",
pages = "1169-1175",
doi = "10.1055/s-2007-981586"
}

Leskovac, A., Joksić, G., Janković, T., Savildn, K.,& Menkovic, N.. (2007). Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro. in Planta Medica, 73(11), 1169-1175.
https://doi.org/10.1055/s-2007-981586

Leskovac A, Joksić G, Janković T, Savildn K, Menkovic N. Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro. in Planta Medica. 2007;73(11):1169-1175.
doi:10.1055/s-2007-981586 .

Leskovac, Andreja, Joksić, Gordana, Janković, Teodora, Savildn, Katarina, Menkovic, Nebojsa, "Radioprotective properties of the phytochemically characterized extracts of Crataegus monogyna, Cornus mas and Gentianella austriaca on human lymphocytes in vitro" in Planta Medica, 73, no. 11 (2007):1169-1175,
https://doi.org/10.1055/s-2007-981586 . .