TY  - JOUR
AU  - Mićović, Žarko
AU  - Kostić, Sanja
AU  - Mutavdžin, Slavica
AU  - Andrejević, Aleksa
AU  - Stamenković, Aleksandra
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Đurić, Marko
AU  - Hrnčić, Dragan
AU  - Živković, Vladimir
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan
PY  - 2020
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12970
AB  - Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1–0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function.
T2  - Vojnosanitetski pregled
T1  - The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue
VL  - 77
IS  - 2
SP  - 165
EP  - 173
DO  - 10.2298/VSP171213055M
ER  - 

@article{
author = "Mićović, Žarko and Kostić, Sanja and Mutavdžin, Slavica and Andrejević, Aleksa and Stamenković, Aleksandra and Čolović, Mirjana and Krstić, Danijela and Đurić, Marko and Hrnčić, Dragan and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan",
year = "2020",
abstract = "Background/Aim. Chronically induced hypermethioninemia leads to hyperhomocysteinemia which causes oxidative stress, atherogenesis, neurodegeneration and cancer. However, little is known about the acute and subchronic effects of DL-methionine (Met). The aim of study was to assess the effects of acutely and subchronically applied Met on oxidative stress parameters in rat plasma [enzymes: catalase (CAT), glutathione peroxidise (GPx), superoxide dismutase (SOD) and index of lipid peroxidation, malondialdehyde (MDA)], and acetylcholinesterase (AChE) activity in rat cardiac tissue. Methods. The enzymes activities, as well as MDA concentration were evaluated following acute (n = 8) and subchronic (n = 10) application of Met [i.p. 0.8 mmoL/kg body weight (b.w.) in a single dose in the acute overload or daily during three weeks in the subchronic overload]. The same was done in the control groups following application of physiological solution [i.p. 1 mL 0.9% NaCl (n = 8) in the acute overload and 0.1–0.2 mL 0.9% NaCl, daily during three weeks (n =10) in the subchronic overload]. Tested parameters were evaluated 60 minutes after application in acute experiments and after three weeks of treatment in subchronic experiments. Results. There were no difference in homocysteine values between the groups treated with Met for three weeks and the control group. Met administration significantly increased the activity of CAT and GPx after 1 h compared to the control group (p = 0.008 for both enzymes), whereas the activity of SOD and MDA concentrations were unchanged. Subchronically applied Met did not affect activity of antioxidant enzymes and MDA level. AChE activity did not show any change in rat cardiac tissue after 1 h, but it was significantly decreased after the subchronic treatment (p = 0.041). Conclusion. Results of present research indicate that Met differently affects estimated parameters during acute and subchronic application. In the acute treatment Met mobilizes the most part of antioxidant enzymes while during the subchronic treatment these changes seems to be lost. On the contrary, the acute Met overload was not sufficient to influence on the AChE activity, while longer duration of Met loading diminished function of the enzyme. These findings point out that methionine can interfere with antioxidant defense system and cholinergic control of the heart function.",
journal = "Vojnosanitetski pregled",
title = "The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue",
volume = "77",
number = "2",
pages = "165-173",
doi = "10.2298/VSP171213055M"
}

Mićović, Ž., Kostić, S., Mutavdžin, S., Andrejević, A., Stamenković, A., Čolović, M., Krstić, D., Đurić, M., Hrnčić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2020). The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue. in Vojnosanitetski pregled, 77(2), 165-173.
https://doi.org/10.2298/VSP171213055M

Mićović Ž, Kostić S, Mutavdžin S, Andrejević A, Stamenković A, Čolović M, Krstić D, Đurić M, Hrnčić D, Živković V, Jakovljević V, Đurić D. The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue. in Vojnosanitetski pregled. 2020;77(2):165-173.
doi:10.2298/VSP171213055M .

Mićović, Žarko, Kostić, Sanja, Mutavdžin, Slavica, Andrejević, Aleksa, Stamenković, Aleksandra, Čolović, Mirjana, Krstić, Danijela, Đurić, Marko, Hrnčić, Dragan, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of acutely and subchronically applied DL-methionine on plasma oxidative stress markers and activity of acetylcholinesterase in rat cardiac tissue" in Vojnosanitetski pregled, 77, no. 2 (2020):165-173,
https://doi.org/10.2298/VSP171213055M . .

TY  - JOUR
AU  - Đurić, Marko
AU  - Mutavdžin, Slavica
AU  - Lončar-Stojiljković, Dragana
AU  - Kostić, Sanja
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Živković, Vladimir
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12972
AB  - Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.
T2  - Scripta Medica
T1  - The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity
VL  - 50
IS  - 3
SP  - 112
EP  - 116
DO  - 10.5937/scriptamed50-22658
ER  - 

@article{
author = "Đurić, Marko and Mutavdžin, Slavica and Lončar-Stojiljković, Dragana and Kostić, Sanja and Čolović, Mirjana and Krstić, Danijela and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan",
year = "2019",
abstract = "Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.",
journal = "Scripta Medica",
title = "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity",
volume = "50",
number = "3",
pages = "112-116",
doi = "10.5937/scriptamed50-22658"
}

Đurić, M., Mutavdžin, S., Lončar-Stojiljković, D., Kostić, S., Čolović, M., Krstić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2019). The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica, 50(3), 112-116.
https://doi.org/10.5937/scriptamed50-22658

Đurić M, Mutavdžin S, Lončar-Stojiljković D, Kostić S, Čolović M, Krstić D, Živković V, Jakovljević V, Đurić D. The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica. 2019;50(3):112-116.
doi:10.5937/scriptamed50-22658 .

Đurić, Marko, Mutavdžin, Slavica, Lončar-Stojiljković, Dragana, Kostić, Sanja, Čolović, Mirjana, Krstić, Danijela, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity" in Scripta Medica, 50, no. 3 (2019):112-116,
https://doi.org/10.5937/scriptamed50-22658 . .

TY  - JOUR
AU  - Milovanović, Petar
AU  - Rakočević, Zlatko Lj.
AU  - Djonic, Danijela
AU  - Živković, Vladimir
AU  - Hahn, Michael
AU  - Nikolic, Slobodan
AU  - Amling, Michael
AU  - Busse, Bjoern
AU  - Đurić, Marija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6001
AB  - To unravel the origins of decreased bone strength in the superolateral femoral neck, we assessed bone structural features across multiple length scales at this cortical fracture initiating region in postmenopausal women with hip fracture and in aged-matched controls. Our combined methodological approach encompassed atomic force microscopy (AFM) characterization of cortical bone nano-structure, assessment of mineral content/distribution via quantitative backscattered electron imaging (qBEI), measurement of bone material properties by reference point indentation, aswell as evaluation of cortical micro-architecture and osteocyte lacunar density. Our findings revealed a wide range of differences between the fracture group and the controls, suggesting a number of detrimental changes at various levels of cortical bone hierarchical organization that may render bone fragile. Namely, mineral crystals at external cortical bone surfaces of the fracture group were larger (65.22 nm +/- 41.21 nm vs. 36.75 nm +/- 18.49 nm, p LT 0.001), and a shift to a higher mineral content and more homogenous mineralization profile as revealed via qBEI were found in the bone matrix of the fracture group. Fracture cases showed nearly 35% higher cortical porosity and showed significantly reduced osteocyte lacunar density compared to controls (226 +/- 27 vs. 247 +/- 32 #/mm2, p= 0.05). Along with increased crystal size, a shift towards higher mineralization and a tendency to increased cortical porosity and reduced osteocyte lacunar number delineate that cortical bone of the superolateral femoral neck bears distinct signs of fragility at various levels of its structural organization. These results contribute to the understanding of hierarchical bone structure changes in age-related fragility. (C) 2014 Elsevier Inc. All rights reserved.
T2  - Experimental Gerontology
T1  - Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls
VL  - 55
SP  - 19
EP  - 28
DO  - 10.1016/j.exger.2014.03.001
ER  - 

@article{
author = "Milovanović, Petar and Rakočević, Zlatko Lj. and Djonic, Danijela and Živković, Vladimir and Hahn, Michael and Nikolic, Slobodan and Amling, Michael and Busse, Bjoern and Đurić, Marija",
year = "2014",
abstract = "To unravel the origins of decreased bone strength in the superolateral femoral neck, we assessed bone structural features across multiple length scales at this cortical fracture initiating region in postmenopausal women with hip fracture and in aged-matched controls. Our combined methodological approach encompassed atomic force microscopy (AFM) characterization of cortical bone nano-structure, assessment of mineral content/distribution via quantitative backscattered electron imaging (qBEI), measurement of bone material properties by reference point indentation, aswell as evaluation of cortical micro-architecture and osteocyte lacunar density. Our findings revealed a wide range of differences between the fracture group and the controls, suggesting a number of detrimental changes at various levels of cortical bone hierarchical organization that may render bone fragile. Namely, mineral crystals at external cortical bone surfaces of the fracture group were larger (65.22 nm +/- 41.21 nm vs. 36.75 nm +/- 18.49 nm, p LT 0.001), and a shift to a higher mineral content and more homogenous mineralization profile as revealed via qBEI were found in the bone matrix of the fracture group. Fracture cases showed nearly 35% higher cortical porosity and showed significantly reduced osteocyte lacunar density compared to controls (226 +/- 27 vs. 247 +/- 32 #/mm2, p= 0.05). Along with increased crystal size, a shift towards higher mineralization and a tendency to increased cortical porosity and reduced osteocyte lacunar number delineate that cortical bone of the superolateral femoral neck bears distinct signs of fragility at various levels of its structural organization. These results contribute to the understanding of hierarchical bone structure changes in age-related fragility. (C) 2014 Elsevier Inc. All rights reserved.",
journal = "Experimental Gerontology",
title = "Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls",
volume = "55",
pages = "19-28",
doi = "10.1016/j.exger.2014.03.001"
}

Milovanović, P., Rakočević, Z. Lj., Djonic, D., Živković, V., Hahn, M., Nikolic, S., Amling, M., Busse, B.,& Đurić, M.. (2014). Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls. in Experimental Gerontology, 55, 19-28.
https://doi.org/10.1016/j.exger.2014.03.001

Milovanović P, Rakočević ZL, Djonic D, Živković V, Hahn M, Nikolic S, Amling M, Busse B, Đurić M. Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls. in Experimental Gerontology. 2014;55:19-28.
doi:10.1016/j.exger.2014.03.001 .

Milovanović, Petar, Rakočević, Zlatko Lj., Djonic, Danijela, Živković, Vladimir, Hahn, Michael, Nikolic, Slobodan, Amling, Michael, Busse, Bjoern, Đurić, Marija, "Nano-structural, compositional and micro-architectural signs of cortical bone fragility at the superolateral femoral neck in elderly hip fracture patients vs. healthy aged controls" in Experimental Gerontology, 55 (2014):19-28,
https://doi.org/10.1016/j.exger.2014.03.001 . .

TY  - JOUR
AU  - Zelic, Ksenija
AU  - Djonic, Danijela
AU  - Nešković, Olivera M.
AU  - Stoiljković, Milovan
AU  - Nikolic, Slobodan
AU  - Živković, Vladimir
AU  - Đurić, Marija
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5654
AB  - In 2011, small mass grave with completely skeletonized remains was discovered in Belgrade suburb. An eyewitness claimed that skeletons belonged to German soldiers killed in WWII. Anthropologists were engaged to investigate whether the skeletal remains correspond to the indicated German group or represent more recent case requiring court trial. Numerous dental restorations were noticed. Owing to the fact that different dental materials were used in dental practice at certain times, the aim of this study was to explore whether analysis of dental restorations could help in identification and estimation of time since death. Inductively coupled plasma optical emission spectrometry revealed that dental fillings corresponded to copper amalgam, conventional silver amalgam, silicophosphate cement, and zinc phosphate cement. Chemical results combined with anthropological and historical facts suggest that the individuals lived before the 1960s in country with well-developed dental service at that time. Therefore, chemical analysis of dental fillings was useful to distinguish between skeletal remains that are too old to be of forensic interest and the remains relevant to legal investigations.
T2  - Journal of Forensic Sciences
T1  - Forensic or Archaeological Issue: Is Chemical Analysis of Dental Restorations Helpful in Assessing Time Since Death and Identification of Skeletonized Human Remains?
VL  - 58
IS  - 5
SP  - 1284
EP  - 1288
DO  - 10.1111/1556-4029.12228
ER  - 

@article{
author = "Zelic, Ksenija and Djonic, Danijela and Nešković, Olivera M. and Stoiljković, Milovan and Nikolic, Slobodan and Živković, Vladimir and Đurić, Marija",
year = "2013",
abstract = "In 2011, small mass grave with completely skeletonized remains was discovered in Belgrade suburb. An eyewitness claimed that skeletons belonged to German soldiers killed in WWII. Anthropologists were engaged to investigate whether the skeletal remains correspond to the indicated German group or represent more recent case requiring court trial. Numerous dental restorations were noticed. Owing to the fact that different dental materials were used in dental practice at certain times, the aim of this study was to explore whether analysis of dental restorations could help in identification and estimation of time since death. Inductively coupled plasma optical emission spectrometry revealed that dental fillings corresponded to copper amalgam, conventional silver amalgam, silicophosphate cement, and zinc phosphate cement. Chemical results combined with anthropological and historical facts suggest that the individuals lived before the 1960s in country with well-developed dental service at that time. Therefore, chemical analysis of dental fillings was useful to distinguish between skeletal remains that are too old to be of forensic interest and the remains relevant to legal investigations.",
journal = "Journal of Forensic Sciences",
title = "Forensic or Archaeological Issue: Is Chemical Analysis of Dental Restorations Helpful in Assessing Time Since Death and Identification of Skeletonized Human Remains?",
volume = "58",
number = "5",
pages = "1284-1288",
doi = "10.1111/1556-4029.12228"
}

Zelic, K., Djonic, D., Nešković, O. M., Stoiljković, M., Nikolic, S., Živković, V.,& Đurić, M.. (2013). Forensic or Archaeological Issue: Is Chemical Analysis of Dental Restorations Helpful in Assessing Time Since Death and Identification of Skeletonized Human Remains?. in Journal of Forensic Sciences, 58(5), 1284-1288.
https://doi.org/10.1111/1556-4029.12228

Zelic K, Djonic D, Nešković OM, Stoiljković M, Nikolic S, Živković V, Đurić M. Forensic or Archaeological Issue: Is Chemical Analysis of Dental Restorations Helpful in Assessing Time Since Death and Identification of Skeletonized Human Remains?. in Journal of Forensic Sciences. 2013;58(5):1284-1288.
doi:10.1111/1556-4029.12228 .

Zelic, Ksenija, Djonic, Danijela, Nešković, Olivera M., Stoiljković, Milovan, Nikolic, Slobodan, Živković, Vladimir, Đurić, Marija, "Forensic or Archaeological Issue: Is Chemical Analysis of Dental Restorations Helpful in Assessing Time Since Death and Identification of Skeletonized Human Remains?" in Journal of Forensic Sciences, 58, no. 5 (2013):1284-1288,
https://doi.org/10.1111/1556-4029.12228 . .

TY  - JOUR
AU  - Milovanović, Petar
AU  - Đurić, Marija
AU  - Nešković, Olivera M.
AU  - Djonic, Danijela
AU  - Potočnik, Jelena
AU  - Nikolic, Slobodan
AU  - Stoiljković, Milovan
AU  - Živković, Vladimir
AU  - Rakočević, Zlatko Lj.
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5687
AB  - On the basis of the suggestion that bone nanostructure bears tissue age information and may reflect surface deposition/modification processes, we performed nanoscale characterization of the external cortical bone surface at the femoral neck in women using atomic force microscopy (AFM). The specific aims were to assess age-related differences in bone nanostructure and explore the existence of nanostructural traces of potential bone apposition at this surface. Our findings revealed that the external cortical surface represents a continuous phase composed of densely packed mineral grains. Although the grains varied in size and shape, there was a domination of small grains indicative of freshly deposited bone (mean grain size: young, 35 nm; old, 37 nm; p. 0.05). Advanced quantitative analysis of surface morphological patterns revealed comparable roughness and complexity of the surface, suggesting a similar rate of mineral particle deposition at the surface in both groups. Calcium/phosphorus ratio, a measure of bone tissue age, was within the same range in both groups. In summary, our AFM analyses showed consistent nanostructural and compositional bone features, suggesting existence of new bone at the periosteal bone surface in both young and elderly women. Considering observed age-related increase in the neck diameter, AFM findings may support the theory of continuous bone apposition at the periosteal surface.
T2  - Microscopy and Microanalysis
T1  - Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging
VL  - 19
IS  - 5
SP  - 1341
EP  - 1349
DO  - 10.1017/S1431927613001761
ER  - 

@article{
author = "Milovanović, Petar and Đurić, Marija and Nešković, Olivera M. and Djonic, Danijela and Potočnik, Jelena and Nikolic, Slobodan and Stoiljković, Milovan and Živković, Vladimir and Rakočević, Zlatko Lj.",
year = "2013",
abstract = "On the basis of the suggestion that bone nanostructure bears tissue age information and may reflect surface deposition/modification processes, we performed nanoscale characterization of the external cortical bone surface at the femoral neck in women using atomic force microscopy (AFM). The specific aims were to assess age-related differences in bone nanostructure and explore the existence of nanostructural traces of potential bone apposition at this surface. Our findings revealed that the external cortical surface represents a continuous phase composed of densely packed mineral grains. Although the grains varied in size and shape, there was a domination of small grains indicative of freshly deposited bone (mean grain size: young, 35 nm; old, 37 nm; p. 0.05). Advanced quantitative analysis of surface morphological patterns revealed comparable roughness and complexity of the surface, suggesting a similar rate of mineral particle deposition at the surface in both groups. Calcium/phosphorus ratio, a measure of bone tissue age, was within the same range in both groups. In summary, our AFM analyses showed consistent nanostructural and compositional bone features, suggesting existence of new bone at the periosteal bone surface in both young and elderly women. Considering observed age-related increase in the neck diameter, AFM findings may support the theory of continuous bone apposition at the periosteal surface.",
journal = "Microscopy and Microanalysis",
title = "Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging",
volume = "19",
number = "5",
pages = "1341-1349",
doi = "10.1017/S1431927613001761"
}

Milovanović, P., Đurić, M., Nešković, O. M., Djonic, D., Potočnik, J., Nikolic, S., Stoiljković, M., Živković, V.,& Rakočević, Z. Lj.. (2013). Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging. in Microscopy and Microanalysis, 19(5), 1341-1349.
https://doi.org/10.1017/S1431927613001761

Milovanović P, Đurić M, Nešković OM, Djonic D, Potočnik J, Nikolic S, Stoiljković M, Živković V, Rakočević ZL. Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging. in Microscopy and Microanalysis. 2013;19(5):1341-1349.
doi:10.1017/S1431927613001761 .

Milovanović, Petar, Đurić, Marija, Nešković, Olivera M., Djonic, Danijela, Potočnik, Jelena, Nikolic, Slobodan, Stoiljković, Milovan, Živković, Vladimir, Rakočević, Zlatko Lj., "Atomic Force Microscopy Characterization of the External Cortical Bone Surface in Young and Elderly Women: Potential Nanostructural Traces of Periosteal Bone Apposition During Aging" in Microscopy and Microanalysis, 19, no. 5 (2013):1341-1349,
https://doi.org/10.1017/S1431927613001761 . .

TY  - JOUR
AU  - Milovanović, Petar
AU  - Potočnik, Jelena
AU  - Djonic, Danijela
AU  - Nikolic, Slobodan
AU  - Živković, Vladimir
AU  - Đurić, Marija
AU  - Rakočević, Zlatko Lj.
PY  - 2012
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4716
AB  - Despite general belief that the mechanical properties of bone material contribute to whole bone strength, it is still obscure what the age effects are on mechanical behavior of the bone material, particularly in the case of the femoral neck trabeculae. In this study, atomic force microscopy was used for imaging and measuring the size of mineral grains, as well as nano-scale mechanical characterization (nanoindentation) of the bone mineralized matrix of trabeculae, with the aim to explore the age effects on bone elasticity and give new insight into age-related bone fragility. The bone samples in this study comprised trabecular bone specimens of the femoral neck region, collected from eight skeletal healthy women (five young adults: 27-38 yrs., three elderly: 83-94 yrs.) at autopsy. Bone trabeculae in the elderly displayed a higher modulus and nanohardness, signifying a decreased amount of energy that can be accommodated by the bone tissue during loading. Regression analysis revealed that nearly 65% of variability in the bone matrix elastic modulus can be statistically explained by the changes in size of the matrix mineral grains. This study revealed that the bone trabeculae of elderly women express less elastic behavior at the material level, which makes them more vulnerable to unusual impact loads originating from a fall. The observed age-related structural and mechanical alteration at the bone material level adds new evidence for understanding why hip fractures are more frequent in elderly women. (C) 2011 Elsevier Inc. All rights reserved.
T2  - Experimental Gerontology
T1  - Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM
VL  - 47
IS  - 2
SP  - 154
EP  - 159
DO  - 10.1016/j.exger.2011.11.011
ER  - 

@article{
author = "Milovanović, Petar and Potočnik, Jelena and Djonic, Danijela and Nikolic, Slobodan and Živković, Vladimir and Đurić, Marija and Rakočević, Zlatko Lj.",
year = "2012",
abstract = "Despite general belief that the mechanical properties of bone material contribute to whole bone strength, it is still obscure what the age effects are on mechanical behavior of the bone material, particularly in the case of the femoral neck trabeculae. In this study, atomic force microscopy was used for imaging and measuring the size of mineral grains, as well as nano-scale mechanical characterization (nanoindentation) of the bone mineralized matrix of trabeculae, with the aim to explore the age effects on bone elasticity and give new insight into age-related bone fragility. The bone samples in this study comprised trabecular bone specimens of the femoral neck region, collected from eight skeletal healthy women (five young adults: 27-38 yrs., three elderly: 83-94 yrs.) at autopsy. Bone trabeculae in the elderly displayed a higher modulus and nanohardness, signifying a decreased amount of energy that can be accommodated by the bone tissue during loading. Regression analysis revealed that nearly 65% of variability in the bone matrix elastic modulus can be statistically explained by the changes in size of the matrix mineral grains. This study revealed that the bone trabeculae of elderly women express less elastic behavior at the material level, which makes them more vulnerable to unusual impact loads originating from a fall. The observed age-related structural and mechanical alteration at the bone material level adds new evidence for understanding why hip fractures are more frequent in elderly women. (C) 2011 Elsevier Inc. All rights reserved.",
journal = "Experimental Gerontology",
title = "Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM",
volume = "47",
number = "2",
pages = "154-159",
doi = "10.1016/j.exger.2011.11.011"
}

Milovanović, P., Potočnik, J., Djonic, D., Nikolic, S., Živković, V., Đurić, M.,& Rakočević, Z. Lj.. (2012). Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM. in Experimental Gerontology, 47(2), 154-159.
https://doi.org/10.1016/j.exger.2011.11.011

Milovanović P, Potočnik J, Djonic D, Nikolic S, Živković V, Đurić M, Rakočević ZL. Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM. in Experimental Gerontology. 2012;47(2):154-159.
doi:10.1016/j.exger.2011.11.011 .

Milovanović, Petar, Potočnik, Jelena, Djonic, Danijela, Nikolic, Slobodan, Živković, Vladimir, Đurić, Marija, Rakočević, Zlatko Lj., "Age-related deterioration in trabecular bone mechanical properties at material level: Nanoindentation study of the femoral neck in women by using AFM" in Experimental Gerontology, 47, no. 2 (2012):154-159,
https://doi.org/10.1016/j.exger.2011.11.011 . .