The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity
Аутори
Đurić, MarkoMutavdžin, Slavica
Lončar-Stojiljković, Dragana
Kostić, Sanja
Čolović, Mirjana
Krstić, Danijela
Živković, Vladimir
Jakovljević, Vladimir
Đurić, Dragan
Чланак у часопису (Објављена верзија)
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Приказ свих података о документуАпстракт
Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured i...n the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.
Кључне речи:
acetylcholinesterase / heart / homocysteine / inhibitionИзвор:
Scripta Medica, 2019, 50, 3, 112-116Финансирање / пројекти:
- Ефекти хомоцистеина и хомоцистеину сродних супстанци на кардиоваскуларни систем: Улога гасних трансмитера No, H2S и CO (RS-MESTD-Basic Research (BR or ON)-175043)
- COST action [CA16225]
Колекције
Институција/група
VinčaTY - JOUR AU - Đurić, Marko AU - Mutavdžin, Slavica AU - Lončar-Stojiljković, Dragana AU - Kostić, Sanja AU - Čolović, Mirjana AU - Krstić, Danijela AU - Živković, Vladimir AU - Jakovljević, Vladimir AU - Đurić, Dragan PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12972 AB - Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters. T2 - Scripta Medica T1 - The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity VL - 50 IS - 3 SP - 112 EP - 116 DO - 10.5937/scriptamed50-22658 ER -
@article{ author = "Đurić, Marko and Mutavdžin, Slavica and Lončar-Stojiljković, Dragana and Kostić, Sanja and Čolović, Mirjana and Krstić, Danijela and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan", year = "2019", abstract = "Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.", journal = "Scripta Medica", title = "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity", volume = "50", number = "3", pages = "112-116", doi = "10.5937/scriptamed50-22658" }
Đurić, M., Mutavdžin, S., Lončar-Stojiljković, D., Kostić, S., Čolović, M., Krstić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2019). The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica, 50(3), 112-116. https://doi.org/10.5937/scriptamed50-22658
Đurić M, Mutavdžin S, Lončar-Stojiljković D, Kostić S, Čolović M, Krstić D, Živković V, Jakovljević V, Đurić D. The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica. 2019;50(3):112-116. doi:10.5937/scriptamed50-22658 .
Đurić, Marko, Mutavdžin, Slavica, Lončar-Stojiljković, Dragana, Kostić, Sanja, Čolović, Mirjana, Krstić, Danijela, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity" in Scripta Medica, 50, no. 3 (2019):112-116, https://doi.org/10.5937/scriptamed50-22658 . .