Mrmošanin, Jelena

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Antioxidant Activity of Grapefruit (Citrus Paradisi) Extract

Miljković, Vojkan; Mrmošanin, Jelena; Zvezdanović, Jelena; Momčilović, Milan Z.; Miljković, Milena; Mihajlov-Krstev, Tatjana; Nikolić, Ljubiša

(Leskovac : Faculty of Technology, University of Niš, 2023)

TY  - CONF
AU  - Miljković, Vojkan
AU  - Mrmošanin, Jelena
AU  - Zvezdanović, Jelena
AU  - Momčilović, Milan Z.
AU  - Miljković, Milena
AU  - Mihajlov-Krstev, Tatjana
AU  - Nikolić, Ljubiša
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12250
AB  - Citrus fruits are widely consumed around the world and among them grapefruit (Citrus paradisi macfad) stands out with its characteristic appearance and flavor. The fruit is growing on a tree that belongs to Rutaceae family and its positive contribution to health is attributed to phytochemicals found in it. The phytochemicals have the key role in mediating oxidation process because they act as antioxidants and by that prevention of cell damage, reducing the risks of many diseases, boosting of the immune system, balancing hormone levels, and notable antibacterial and antiviral response of the organism happens. Grapefruits contain several phytochemicals including: carotenoids, flavonoids (naringin), limonoids, organic acids, pectin, and folate. Considering the worldwide consumption and popularity in everyday diet of grapefruit, the antioxidant activity of its extracts is determined in this paper. Weighed amount 10 g of fresh grapefruits was added to a mixture consisting of organic solvents (hexane, acetone, and ethanol in volumes ratio 2:1:1, respectively) and 0.05% (w/v) butylated hydroxytoluene. The mixture was stoppered and mixed on an orbital shaker at 180 rpm for 15 minutes. The temperature during the mixing was kept at 5 °C in the water bath. Afterward, 7.5 ml of cold deionized water on every 10 grams of starting sample was added to the mixture and agitated for another 5 min. The suspension was transferred to a separation funnel to separate the upper (non-polar) from the lower (polar) phase for 10 minutes at room temperature. The polar phase was subjected for antioxidant capacity determination by ABTS, DPPH, FRAP and CUPRAC methods. The results for the applied methods expressed per kg of the sample are: 1.8 mmol∙kg-1, 0.72 mmol∙kg-1, 9.7 μmol∙kg-1, 4.66 mmol∙kg-1, respectively. The obtained results are recommending grapefruit as a potent source of phytochemicals with antioxidant properties.
PB  - Leskovac : Faculty of Technology, University of Niš
C3  - 15th International symposium „Novel technologies and sustainable development" : Book of Abstracts
T1  - Antioxidant Activity of Grapefruit (Citrus Paradisi) Extract
SP  - 70
EP  - 70
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12250
ER  - 
@conference{
author = "Miljković, Vojkan and Mrmošanin, Jelena and Zvezdanović, Jelena and Momčilović, Milan Z. and Miljković, Milena and Mihajlov-Krstev, Tatjana and Nikolić, Ljubiša",
year = "2023",
abstract = "Citrus fruits are widely consumed around the world and among them grapefruit (Citrus paradisi macfad) stands out with its characteristic appearance and flavor. The fruit is growing on a tree that belongs to Rutaceae family and its positive contribution to health is attributed to phytochemicals found in it. The phytochemicals have the key role in mediating oxidation process because they act as antioxidants and by that prevention of cell damage, reducing the risks of many diseases, boosting of the immune system, balancing hormone levels, and notable antibacterial and antiviral response of the organism happens. Grapefruits contain several phytochemicals including: carotenoids, flavonoids (naringin), limonoids, organic acids, pectin, and folate. Considering the worldwide consumption and popularity in everyday diet of grapefruit, the antioxidant activity of its extracts is determined in this paper. Weighed amount 10 g of fresh grapefruits was added to a mixture consisting of organic solvents (hexane, acetone, and ethanol in volumes ratio 2:1:1, respectively) and 0.05% (w/v) butylated hydroxytoluene. The mixture was stoppered and mixed on an orbital shaker at 180 rpm for 15 minutes. The temperature during the mixing was kept at 5 °C in the water bath. Afterward, 7.5 ml of cold deionized water on every 10 grams of starting sample was added to the mixture and agitated for another 5 min. The suspension was transferred to a separation funnel to separate the upper (non-polar) from the lower (polar) phase for 10 minutes at room temperature. The polar phase was subjected for antioxidant capacity determination by ABTS, DPPH, FRAP and CUPRAC methods. The results for the applied methods expressed per kg of the sample are: 1.8 mmol∙kg-1, 0.72 mmol∙kg-1, 9.7 μmol∙kg-1, 4.66 mmol∙kg-1, respectively. The obtained results are recommending grapefruit as a potent source of phytochemicals with antioxidant properties.",
publisher = "Leskovac : Faculty of Technology, University of Niš",
journal = "15th International symposium „Novel technologies and sustainable development" : Book of Abstracts",
title = "Antioxidant Activity of Grapefruit (Citrus Paradisi) Extract",
pages = "70-70",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12250"
}
Miljković, V., Mrmošanin, J., Zvezdanović, J., Momčilović, M. Z., Miljković, M., Mihajlov-Krstev, T.,& Nikolić, L.. (2023). Antioxidant Activity of Grapefruit (Citrus Paradisi) Extract. in 15th International symposium „Novel technologies and sustainable development" : Book of Abstracts
Leskovac : Faculty of Technology, University of Niš., 70-70.
https://hdl.handle.net/21.15107/rcub_vinar_12250
Miljković V, Mrmošanin J, Zvezdanović J, Momčilović MZ, Miljković M, Mihajlov-Krstev T, Nikolić L. Antioxidant Activity of Grapefruit (Citrus Paradisi) Extract. in 15th International symposium „Novel technologies and sustainable development" : Book of Abstracts. 2023;:70-70.
https://hdl.handle.net/21.15107/rcub_vinar_12250 .
Miljković, Vojkan, Mrmošanin, Jelena, Zvezdanović, Jelena, Momčilović, Milan Z., Miljković, Milena, Mihajlov-Krstev, Tatjana, Nikolić, Ljubiša, "Antioxidant Activity of Grapefruit (Citrus Paradisi) Extract" in 15th International symposium „Novel technologies and sustainable development" : Book of Abstracts (2023):70-70,
https://hdl.handle.net/21.15107/rcub_vinar_12250 .

Carotenoid and flavonoid levels, antioxidant activity and antimicrobial properties of tomato grown in Serbia

Miljković, Vojkan M.; Momčilović, Milan Z.; Zvezdanović, Jelena B.; Gajić, Ivana Lj.; Mrmošanin, Jelena M.; Mihajlov-Krstev, Tatjana

(2022)

TY  - JOUR
AU  - Miljković, Vojkan M.
AU  - Momčilović, Milan Z.
AU  - Zvezdanović, Jelena B.
AU  - Gajić, Ivana Lj.
AU  - Mrmošanin, Jelena M.
AU  - Mihajlov-Krstev, Tatjana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10553
AB  - This work provides an insight into the nutritional quality of Hector-F1 tomato (Solanum lycopersicum L.) fruit grown in Serbia and the juice prepared from it regarding the contents of carotenoids, flavonoids and polyphenols as compounds beneficial for a healthy diet. Initially, ultra-high performance liquid chromatography with electrospray ionization mass spectrometry (UHPLC-ESI-MS) was employed for both polar and non-polar tomato extracts to identify compounds present in the samples. Then, lycopene, β-carotene, total polyphenols and flavonoids contents were determined by means of ultraviolet-visible (UV-Vis) spectrophotometry. To evaluate the antioxidant activity of the samples, four methods were applied, namely, the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method, 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, the ferric ion reducing antioxidant power (FRAP) method and the cupric ion reducing antioxidant capacity (CUPRAC) method. In addition, the antimicrobial potential was tested against gram-positive bacteria, gram-negative bacteria and the yeast Candida albicans. The thermal treatment in all cases modified the chemical composition of tomato as demonstrated by a decrease in values of all measured parameters. This comprehensive analysis provided data applicable to profiling and comparing cultivars grown in different geographical regions. © 2022 National Agricultural and Food Centre (Slovakia).
T2  - Journal of Food and Nutrition Research
T1  - Carotenoid and flavonoid levels, antioxidant activity and antimicrobial properties of tomato grown in Serbia
VL  - 61
IS  - 4
SP  - 402
EP  - 414
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10553
ER  - 
@article{
author = "Miljković, Vojkan M. and Momčilović, Milan Z. and Zvezdanović, Jelena B. and Gajić, Ivana Lj. and Mrmošanin, Jelena M. and Mihajlov-Krstev, Tatjana",
year = "2022",
abstract = "This work provides an insight into the nutritional quality of Hector-F1 tomato (Solanum lycopersicum L.) fruit grown in Serbia and the juice prepared from it regarding the contents of carotenoids, flavonoids and polyphenols as compounds beneficial for a healthy diet. Initially, ultra-high performance liquid chromatography with electrospray ionization mass spectrometry (UHPLC-ESI-MS) was employed for both polar and non-polar tomato extracts to identify compounds present in the samples. Then, lycopene, β-carotene, total polyphenols and flavonoids contents were determined by means of ultraviolet-visible (UV-Vis) spectrophotometry. To evaluate the antioxidant activity of the samples, four methods were applied, namely, the 2,2’-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) method, 2,2-diphenyl-1-picrylhydrazyl (DPPH) method, the ferric ion reducing antioxidant power (FRAP) method and the cupric ion reducing antioxidant capacity (CUPRAC) method. In addition, the antimicrobial potential was tested against gram-positive bacteria, gram-negative bacteria and the yeast Candida albicans. The thermal treatment in all cases modified the chemical composition of tomato as demonstrated by a decrease in values of all measured parameters. This comprehensive analysis provided data applicable to profiling and comparing cultivars grown in different geographical regions. © 2022 National Agricultural and Food Centre (Slovakia).",
journal = "Journal of Food and Nutrition Research",
title = "Carotenoid and flavonoid levels, antioxidant activity and antimicrobial properties of tomato grown in Serbia",
volume = "61",
number = "4",
pages = "402-414",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10553"
}
Miljković, V. M., Momčilović, M. Z., Zvezdanović, J. B., Gajić, I. Lj., Mrmošanin, J. M.,& Mihajlov-Krstev, T.. (2022). Carotenoid and flavonoid levels, antioxidant activity and antimicrobial properties of tomato grown in Serbia. in Journal of Food and Nutrition Research, 61(4), 402-414.
https://hdl.handle.net/21.15107/rcub_vinar_10553
Miljković VM, Momčilović MZ, Zvezdanović JB, Gajić IL, Mrmošanin JM, Mihajlov-Krstev T. Carotenoid and flavonoid levels, antioxidant activity and antimicrobial properties of tomato grown in Serbia. in Journal of Food and Nutrition Research. 2022;61(4):402-414.
https://hdl.handle.net/21.15107/rcub_vinar_10553 .
Miljković, Vojkan M., Momčilović, Milan Z., Zvezdanović, Jelena B., Gajić, Ivana Lj., Mrmošanin, Jelena M., Mihajlov-Krstev, Tatjana, "Carotenoid and flavonoid levels, antioxidant activity and antimicrobial properties of tomato grown in Serbia" in Journal of Food and Nutrition Research, 61, no. 4 (2022):402-414,
https://hdl.handle.net/21.15107/rcub_vinar_10553 .