Petrović, S

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  • Petrović, S (5)
  • Petrovic, S (4)
  • Petrović, Spomenka Z. (1)
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Author's Bibliography

Inhibition of rat brain ecto-ATPase activity by various drugs

Horvat, Anica; Orlić, T; Banjac, Ana; Momić, Tatjana; Petrović, S; Demajo, Miroslav

(2006)

TY  - JOUR
AU  - Horvat, Anica
AU  - Orlić, T
AU  - Banjac, Ana
AU  - Momić, Tatjana
AU  - Petrović, S
AU  - Demajo, Miroslav
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3024
AB  - The in vitro effect of digoxin, verapamil, propranolol, carbamazepine, diazepam and promethazine were investigated on the ecto-ATPase activity of synaptosomal plasma membranes from the rat brain. ATP hydrolyzing activities of the enzyme were not affected by digoxin while the use of all other drugs resulted in significant and dose-dependent ihibition in ATP hydrolysis. According to values Of IC50 and K-iapp, the order of inhibitory potency of the drugs applied was: diazepam GT promethazine GT verapamil GT propranolol GT GT carbamazepine. Kinetic analysis of the nature of the ATPase inhibition revealed that it resulted from a direct action of drugs on the enzyme protein. The aim of the present study was to determine the potential neuromodulatory side effects of the drugs investigated. The results achieved indicated that all investigated drugs, except digoxin, may modulate neuronal activities via the purinergic receptors P2 by increasing extracellular concentrations of ATP as a consequence of inhibition of the ecto-ATPase activity. Our findings indicate that it may be useful to take into consideration the possible side effects of the investigated drugs, when they are used in treatment of different pathologies, particularly in the treatment of epilepsy by carbamazepine and diazepam.
T2  - General Physiology and Biophysics
T1  - Inhibition of rat brain ecto-ATPase activity by various drugs
VL  - 25
IS  - 1
SP  - 91
EP  - 105
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3024
ER  - 
@article{
author = "Horvat, Anica and Orlić, T and Banjac, Ana and Momić, Tatjana and Petrović, S and Demajo, Miroslav",
year = "2006",
abstract = "The in vitro effect of digoxin, verapamil, propranolol, carbamazepine, diazepam and promethazine were investigated on the ecto-ATPase activity of synaptosomal plasma membranes from the rat brain. ATP hydrolyzing activities of the enzyme were not affected by digoxin while the use of all other drugs resulted in significant and dose-dependent ihibition in ATP hydrolysis. According to values Of IC50 and K-iapp, the order of inhibitory potency of the drugs applied was: diazepam GT promethazine GT verapamil GT propranolol GT GT carbamazepine. Kinetic analysis of the nature of the ATPase inhibition revealed that it resulted from a direct action of drugs on the enzyme protein. The aim of the present study was to determine the potential neuromodulatory side effects of the drugs investigated. The results achieved indicated that all investigated drugs, except digoxin, may modulate neuronal activities via the purinergic receptors P2 by increasing extracellular concentrations of ATP as a consequence of inhibition of the ecto-ATPase activity. Our findings indicate that it may be useful to take into consideration the possible side effects of the investigated drugs, when they are used in treatment of different pathologies, particularly in the treatment of epilepsy by carbamazepine and diazepam.",
journal = "General Physiology and Biophysics",
title = "Inhibition of rat brain ecto-ATPase activity by various drugs",
volume = "25",
number = "1",
pages = "91-105",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3024"
}
Horvat, A., Orlić, T., Banjac, A., Momić, T., Petrović, S.,& Demajo, M.. (2006). Inhibition of rat brain ecto-ATPase activity by various drugs. in General Physiology and Biophysics, 25(1), 91-105.
https://hdl.handle.net/21.15107/rcub_vinar_3024
Horvat A, Orlić T, Banjac A, Momić T, Petrović S, Demajo M. Inhibition of rat brain ecto-ATPase activity by various drugs. in General Physiology and Biophysics. 2006;25(1):91-105.
https://hdl.handle.net/21.15107/rcub_vinar_3024 .
Horvat, Anica, Orlić, T, Banjac, Ana, Momić, Tatjana, Petrović, S, Demajo, Miroslav, "Inhibition of rat brain ecto-ATPase activity by various drugs" in General Physiology and Biophysics, 25, no. 1 (2006):91-105,
https://hdl.handle.net/21.15107/rcub_vinar_3024 .
10

Radon concentrations in a spa in Serbia

Manic, G; Petrović, S; Vesna, M; Popovic, D; Todorović, Dragana

(2006)

TY  - JOUR
AU  - Manic, G
AU  - Petrović, S
AU  - Vesna, M
AU  - Popovic, D
AU  - Todorović, Dragana
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3012
AB  - The paper presents the results of indoor radon concentration survey in 201 homes and offices in Niska Banja (the Spa of Nis), a well-known health resort and a spa in the South-East of Serbia. Radon indoor concentrations were determined by active charcoal method, according to standard EPA procedure. The indoor radon concentrations were in the range of up to 200 Bq/m(3) (47%), from 200-600 Bq/m(3) (26%) and over 600 Bq/m(3) (27%). Three areas of extremely high average radon concentrations were found (1340-4340 Bq/m(3)), with a maximum above 13,000 Bq/m(3). The content of natural radionuclides (Ra-226, Ph-214, Bi-214, U-235, Ac-228, Ph-212, Bi-212, (TI)-T-208, K-40) and Cs-117, as well as the content of total uranium, thorium and potassium in mud used in peloidotherapy in the Health Institute Niska Banja was determined, too. The activities of the radionuclides were determined on an HPGe detector, by standard gamma spectroscopy. The results indicated considerably high amounts of total uranium and thorium (0.021 g/kg mud and 0.003 g/kg mud, respectively), due to the karsts origin of the soil. (c) 2006 Elsevier Ltd. All rights reserved.
T2  - Environment International
T1  - Radon concentrations in a spa in Serbia
VL  - 32
IS  - 4
SP  - 533
EP  - 537
DO  - 10.1016/j.envint.2005.12.002
ER  - 
@article{
author = "Manic, G and Petrović, S and Vesna, M and Popovic, D and Todorović, Dragana",
year = "2006",
abstract = "The paper presents the results of indoor radon concentration survey in 201 homes and offices in Niska Banja (the Spa of Nis), a well-known health resort and a spa in the South-East of Serbia. Radon indoor concentrations were determined by active charcoal method, according to standard EPA procedure. The indoor radon concentrations were in the range of up to 200 Bq/m(3) (47%), from 200-600 Bq/m(3) (26%) and over 600 Bq/m(3) (27%). Three areas of extremely high average radon concentrations were found (1340-4340 Bq/m(3)), with a maximum above 13,000 Bq/m(3). The content of natural radionuclides (Ra-226, Ph-214, Bi-214, U-235, Ac-228, Ph-212, Bi-212, (TI)-T-208, K-40) and Cs-117, as well as the content of total uranium, thorium and potassium in mud used in peloidotherapy in the Health Institute Niska Banja was determined, too. The activities of the radionuclides were determined on an HPGe detector, by standard gamma spectroscopy. The results indicated considerably high amounts of total uranium and thorium (0.021 g/kg mud and 0.003 g/kg mud, respectively), due to the karsts origin of the soil. (c) 2006 Elsevier Ltd. All rights reserved.",
journal = "Environment International",
title = "Radon concentrations in a spa in Serbia",
volume = "32",
number = "4",
pages = "533-537",
doi = "10.1016/j.envint.2005.12.002"
}
Manic, G., Petrović, S., Vesna, M., Popovic, D.,& Todorović, D.. (2006). Radon concentrations in a spa in Serbia. in Environment International, 32(4), 533-537.
https://doi.org/10.1016/j.envint.2005.12.002
Manic G, Petrović S, Vesna M, Popovic D, Todorović D. Radon concentrations in a spa in Serbia. in Environment International. 2006;32(4):533-537.
doi:10.1016/j.envint.2005.12.002 .
Manic, G, Petrović, S, Vesna, M, Popovic, D, Todorović, Dragana, "Radon concentrations in a spa in Serbia" in Environment International, 32, no. 4 (2006):533-537,
https://doi.org/10.1016/j.envint.2005.12.002 . .
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46

Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries

Milošević, Maja; Petrovic, S; Demajo, Miroslav; Horvat, Anica

(2005)

TY  - JOUR
AU  - Milošević, Maja
AU  - Petrovic, S
AU  - Demajo, Miroslav
AU  - Horvat, Anica
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6556
AB  - The in vitro effects of cadmium and mercury were investigated on the Mg2+-ATPase activity of plasma membranes from the rat ovary and uterus. ATP hydrolyzing activities were significant and dose-dependent-inhibited in both plasma membrane preparations by both metals. According to the IC50 and apparent K-i, Cd2+ was most potent in the ovary, while Hg2+ was most potent in the uterus. In ovaries and uterus,Cd2+ inhibits competitively, while Hg2+ inhibits noncompetitively in both organs. The observed inhibition was a consequence of direct action of the chosen metal ions on the enzyme protein and by decreasing ATP hydrolysis, Hg2+ and Cd2+ may affect mammalian fertility.
T2  - Annals of the New York Academy of Sciences
T1  - Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries
VL  - 1048
SP  - 445
EP  - 448
DO  - 10.1196/annals.1342.061
ER  - 
@article{
author = "Milošević, Maja and Petrovic, S and Demajo, Miroslav and Horvat, Anica",
year = "2005",
abstract = "The in vitro effects of cadmium and mercury were investigated on the Mg2+-ATPase activity of plasma membranes from the rat ovary and uterus. ATP hydrolyzing activities were significant and dose-dependent-inhibited in both plasma membrane preparations by both metals. According to the IC50 and apparent K-i, Cd2+ was most potent in the ovary, while Hg2+ was most potent in the uterus. In ovaries and uterus,Cd2+ inhibits competitively, while Hg2+ inhibits noncompetitively in both organs. The observed inhibition was a consequence of direct action of the chosen metal ions on the enzyme protein and by decreasing ATP hydrolysis, Hg2+ and Cd2+ may affect mammalian fertility.",
journal = "Annals of the New York Academy of Sciences",
title = "Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries",
volume = "1048",
pages = "445-448",
doi = "10.1196/annals.1342.061"
}
Milošević, M., Petrovic, S., Demajo, M.,& Horvat, A.. (2005). Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries. in Annals of the New York Academy of Sciences, 1048, 445-448.
https://doi.org/10.1196/annals.1342.061
Milošević M, Petrovic S, Demajo M, Horvat A. Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries. in Annals of the New York Academy of Sciences. 2005;1048:445-448.
doi:10.1196/annals.1342.061 .
Milošević, Maja, Petrovic, S, Demajo, Miroslav, Horvat, Anica, "Effects of metal ions on plasma membrane Mg2+-ATPase in rat uterus and ovaries" in Annals of the New York Academy of Sciences, 1048 (2005):445-448,
https://doi.org/10.1196/annals.1342.061 . .
5
4
5

Early effects of ionizing radiation on rat brain NTPDase activity

Horvat, Anica; Petrovic, S; Milošević, Maja; Stanojević, Ivana; Demajo, Miroslav

(2005)

TY  - CONF
AU  - Horvat, Anica
AU  - Petrovic, S
AU  - Milošević, Maja
AU  - Stanojević, Ivana
AU  - Demajo, Miroslav
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6563
C3  - FEBS Journal
T1  - Early effects of ionizing radiation on rat brain NTPDase activity
VL  - 272
SP  - 190
EP  - 190
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6563
ER  - 
@conference{
author = "Horvat, Anica and Petrovic, S and Milošević, Maja and Stanojević, Ivana and Demajo, Miroslav",
year = "2005",
journal = "FEBS Journal",
title = "Early effects of ionizing radiation on rat brain NTPDase activity",
volume = "272",
pages = "190-190",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6563"
}
Horvat, A., Petrovic, S., Milošević, M., Stanojević, I.,& Demajo, M.. (2005). Early effects of ionizing radiation on rat brain NTPDase activity. in FEBS Journal, 272, 190-190.
https://hdl.handle.net/21.15107/rcub_vinar_6563
Horvat A, Petrovic S, Milošević M, Stanojević I, Demajo M. Early effects of ionizing radiation on rat brain NTPDase activity. in FEBS Journal. 2005;272:190-190.
https://hdl.handle.net/21.15107/rcub_vinar_6563 .
Horvat, Anica, Petrovic, S, Milošević, Maja, Stanojević, Ivana, Demajo, Miroslav, "Early effects of ionizing radiation on rat brain NTPDase activity" in FEBS Journal, 272 (2005):190-190,
https://hdl.handle.net/21.15107/rcub_vinar_6563 .

SEM and EDX analysis of silicon surface irradiated by TEA CO2 laser

Pongrac, I; Gaković, Biljana M.; Trtica, Milan; Nenadović, T; Petrovic, S

(2001)

TY  - CONF
AU  - Pongrac, I
AU  - Gaković, Biljana M.
AU  - Trtica, Milan
AU  - Nenadović, T
AU  - Petrovic, S
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6363
T1  - SEM and EDX analysis of silicon surface irradiated by TEA CO2 laser
SP  - 323
EP  - 324
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6363
ER  - 
@conference{
author = "Pongrac, I and Gaković, Biljana M. and Trtica, Milan and Nenadović, T and Petrovic, S",
year = "2001",
title = "SEM and EDX analysis of silicon surface irradiated by TEA CO2 laser",
pages = "323-324",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6363"
}
Pongrac, I., Gaković, B. M., Trtica, M., Nenadović, T.,& Petrovic, S.. (2001). SEM and EDX analysis of silicon surface irradiated by TEA CO2 laser. , 323-324.
https://hdl.handle.net/21.15107/rcub_vinar_6363
Pongrac I, Gaković BM, Trtica M, Nenadović T, Petrovic S. SEM and EDX analysis of silicon surface irradiated by TEA CO2 laser. 2001;:323-324.
https://hdl.handle.net/21.15107/rcub_vinar_6363 .
Pongrac, I, Gaković, Biljana M., Trtica, Milan, Nenadović, T, Petrovic, S, "SEM and EDX analysis of silicon surface irradiated by TEA CO2 laser" (2001):323-324,
https://hdl.handle.net/21.15107/rcub_vinar_6363 .
1

Binding of estradiol to synaptosomal mitochondria: physiological significance

Horvat, Anica; Nikezie, G; Petrović, S; Kanazir, Dušan T.

(2001)

TY  - JOUR
AU  - Horvat, Anica
AU  - Nikezie, G
AU  - Petrović, S
AU  - Kanazir, Dušan T.
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2430
AB  - The subsynaptosomal distribution and specific binding of 17 beta -estradiol in vitro to mitochondria isolated from presynaptic nerve endings of female rat brain were examined. 17 beta -Estradiol is (i) distributed unequally in synaptosomes and mitochondria posses the highest capacity to bind estradiol with respect to the available amount of the hormone. (ii) Estradiol binds specifically to isolated synaptosomal mitochondria. A Michaelis-Menten plot of specific binding was sigmoidal within a concentration range of 0.1-5 nM of added estradiol, with a saturation plateau at 3 nM. Binding of higher estradiol concentrations demonstrated an exponential Michaelis-Menten plot, indicating non-specific binding to mitochondria. V-max, and K-m for the sigmoidal-shape range were estimated as 46 +/- 6 fmol of estradiol/mg of mitochondrial proteins and 0.46 +/- 0.07 nM free estradiol respectively. (iii) Esstradiol binding is not affected by the removal of ovaries. The results show that inhibition of Na-dependent Ca2+ efflux from mitochondria by estradiol occurs according to an affinity change of the translocator for Na+, at the same estradiol concentrations that show specific binding to mitochondrial membranes. These data imply that physiological concentrations of estradiol, acting on mitochondrial membrane properties, extragenomically modulate the mitochondrial, and consequently the synaptosomal content of Ca2+, and in that way exert a significant change in nerve cell homeostasis.
T2  - Cellular and Molecular Life Sciences / CMLS
T1  - Binding of estradiol to synaptosomal mitochondria: physiological significance
VL  - 58
IS  - 4
SP  - 636
EP  - 644
DO  - 10.1007/PL00000886
ER  - 
@article{
author = "Horvat, Anica and Nikezie, G and Petrović, S and Kanazir, Dušan T.",
year = "2001",
abstract = "The subsynaptosomal distribution and specific binding of 17 beta -estradiol in vitro to mitochondria isolated from presynaptic nerve endings of female rat brain were examined. 17 beta -Estradiol is (i) distributed unequally in synaptosomes and mitochondria posses the highest capacity to bind estradiol with respect to the available amount of the hormone. (ii) Estradiol binds specifically to isolated synaptosomal mitochondria. A Michaelis-Menten plot of specific binding was sigmoidal within a concentration range of 0.1-5 nM of added estradiol, with a saturation plateau at 3 nM. Binding of higher estradiol concentrations demonstrated an exponential Michaelis-Menten plot, indicating non-specific binding to mitochondria. V-max, and K-m for the sigmoidal-shape range were estimated as 46 +/- 6 fmol of estradiol/mg of mitochondrial proteins and 0.46 +/- 0.07 nM free estradiol respectively. (iii) Esstradiol binding is not affected by the removal of ovaries. The results show that inhibition of Na-dependent Ca2+ efflux from mitochondria by estradiol occurs according to an affinity change of the translocator for Na+, at the same estradiol concentrations that show specific binding to mitochondrial membranes. These data imply that physiological concentrations of estradiol, acting on mitochondrial membrane properties, extragenomically modulate the mitochondrial, and consequently the synaptosomal content of Ca2+, and in that way exert a significant change in nerve cell homeostasis.",
journal = "Cellular and Molecular Life Sciences / CMLS",
title = "Binding of estradiol to synaptosomal mitochondria: physiological significance",
volume = "58",
number = "4",
pages = "636-644",
doi = "10.1007/PL00000886"
}
Horvat, A., Nikezie, G., Petrović, S.,& Kanazir, D. T.. (2001). Binding of estradiol to synaptosomal mitochondria: physiological significance. in Cellular and Molecular Life Sciences / CMLS, 58(4), 636-644.
https://doi.org/10.1007/PL00000886
Horvat A, Nikezie G, Petrović S, Kanazir DT. Binding of estradiol to synaptosomal mitochondria: physiological significance. in Cellular and Molecular Life Sciences / CMLS. 2001;58(4):636-644.
doi:10.1007/PL00000886 .
Horvat, Anica, Nikezie, G, Petrović, S, Kanazir, Dušan T., "Binding of estradiol to synaptosomal mitochondria: physiological significance" in Cellular and Molecular Life Sciences / CMLS, 58, no. 4 (2001):636-644,
https://doi.org/10.1007/PL00000886 . .
21
19
22

Estradiol affect Na-dependent Ca2+ efflux from synaptosomal mitochondria

Horvat, Anica; Petrović, S; Nedeljković, N.; Martinović, Jelena; Nikezić, Gordana S.

(2000)

TY  - JOUR
AU  - Horvat, Anica
AU  - Petrović, S
AU  - Nedeljković, N.
AU  - Martinović, Jelena
AU  - Nikezić, Gordana S.
PY  - 2000
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2353
AB  - The effects of gonadal steroid hormone, 17 beta-estradiol (E-2), in vitro on rat brain mitochondria Ca2+ movement were investigated. Intrasynaptosomal mitochondria Ca2+ uptake via an energy-driven Ca2+ uniporter have K-m = 112.73 +/- 7.3 mu mol.l(-1) and V-max = 21.97 +/- 1.7 nmol Ca-45(2+) mg(-1). Ca2+ release trough a Na+/Ca2+ antiporter was measured with a K-m for Na+ of 43.7 +/- 2.6 mmol.l(-1), and V-max of 1.5 +/- 0.3 nmol Ca-45(2+) mg(-1). Addition of estradiol in preincubation mixture did not affect the uptake of Ca2+ mediated by the ruthenium red-sensitive uniporter, while it produced biphasic effect on Na-dependent Ca2+ efflux. Estradiol at concentrations up to 1 nmol.l(-1) decreased the efflux significantly (63% inhibition with respect to the control), and at concentrations above 10 nmol.l(-1) increased it exponentially. The maximum inhibiting concentration of estradiol (0.5 nmol.l(-1)) increased the affinity of the uniporter (K-m reduced by about 30%), without affecting significantly the capacity (V-max) for Na+. The results presented suggest that estradiol inhibits Na-dependent Ca2+ efflux from mitochondria and acts on mitochondrial retention of Ca2+, which may modulate mitochondrial and consequently synaptosomal content of Ca2+, and in this way exerts its role in the homeostasis of calcium in nerve terminals.
T2  - General Physiology and Biophysics
T1  - Estradiol affect Na-dependent Ca2+ efflux from synaptosomal mitochondria
VL  - 19
IS  - 1
SP  - 59
EP  - 71
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2353
ER  - 
@article{
author = "Horvat, Anica and Petrović, S and Nedeljković, N. and Martinović, Jelena and Nikezić, Gordana S.",
year = "2000",
abstract = "The effects of gonadal steroid hormone, 17 beta-estradiol (E-2), in vitro on rat brain mitochondria Ca2+ movement were investigated. Intrasynaptosomal mitochondria Ca2+ uptake via an energy-driven Ca2+ uniporter have K-m = 112.73 +/- 7.3 mu mol.l(-1) and V-max = 21.97 +/- 1.7 nmol Ca-45(2+) mg(-1). Ca2+ release trough a Na+/Ca2+ antiporter was measured with a K-m for Na+ of 43.7 +/- 2.6 mmol.l(-1), and V-max of 1.5 +/- 0.3 nmol Ca-45(2+) mg(-1). Addition of estradiol in preincubation mixture did not affect the uptake of Ca2+ mediated by the ruthenium red-sensitive uniporter, while it produced biphasic effect on Na-dependent Ca2+ efflux. Estradiol at concentrations up to 1 nmol.l(-1) decreased the efflux significantly (63% inhibition with respect to the control), and at concentrations above 10 nmol.l(-1) increased it exponentially. The maximum inhibiting concentration of estradiol (0.5 nmol.l(-1)) increased the affinity of the uniporter (K-m reduced by about 30%), without affecting significantly the capacity (V-max) for Na+. The results presented suggest that estradiol inhibits Na-dependent Ca2+ efflux from mitochondria and acts on mitochondrial retention of Ca2+, which may modulate mitochondrial and consequently synaptosomal content of Ca2+, and in this way exerts its role in the homeostasis of calcium in nerve terminals.",
journal = "General Physiology and Biophysics",
title = "Estradiol affect Na-dependent Ca2+ efflux from synaptosomal mitochondria",
volume = "19",
number = "1",
pages = "59-71",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2353"
}
Horvat, A., Petrović, S., Nedeljković, N., Martinović, J.,& Nikezić, G. S.. (2000). Estradiol affect Na-dependent Ca2+ efflux from synaptosomal mitochondria. in General Physiology and Biophysics, 19(1), 59-71.
https://hdl.handle.net/21.15107/rcub_vinar_2353
Horvat A, Petrović S, Nedeljković N, Martinović J, Nikezić GS. Estradiol affect Na-dependent Ca2+ efflux from synaptosomal mitochondria. in General Physiology and Biophysics. 2000;19(1):59-71.
https://hdl.handle.net/21.15107/rcub_vinar_2353 .
Horvat, Anica, Petrović, S, Nedeljković, N., Martinović, Jelena, Nikezić, Gordana S., "Estradiol affect Na-dependent Ca2+ efflux from synaptosomal mitochondria" in General Physiology and Biophysics, 19, no. 1 (2000):59-71,
https://hdl.handle.net/21.15107/rcub_vinar_2353 .
18

Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area

Gavrović, Marija; Trtić, Tatjana M.; Vujčić, Zoran; Petrović, Spomenka Z.; Jankov, Ratko M.

(1997)

TY  - JOUR
AU  - Gavrović, Marija
AU  - Trtić, Tatjana M.
AU  - Vujčić, Zoran
AU  - Petrović, Spomenka Z.
AU  - Jankov, Ratko M.
PY  - 1997
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2057
AB  - We investigated extracts of timothy grass pollen from four seasons (1989, 1990, 1991, and 1994) by protein content, SDS-PAGE, immunoblot, RAST, RAST inhibition, and crossed immunoelectrophoresis. Extract of the pollen from 1991 showed the lowest yield in quantitative assays. SDS-PAGE, crossed immunoelectrophoresis, RAST, and RAST inhibition expressed approximately comparable patterns for all extracts except that from 1991. Obviously, the quality of grass pollens, as shown for some ragweed (Ambrosia elatior) pollens depend on year of collection. Our findings are partially in agreement with some earlier examinations of the quality of timothy pollen from different pollen seasons.
T2  - Allergy
T1  - Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area
VL  - 52
IS  - 2
SP  - 210
EP  - 214
DO  - 10.1111/j.1398-9995.1997.tb00977.x
ER  - 
@article{
author = "Gavrović, Marija and Trtić, Tatjana M. and Vujčić, Zoran and Petrović, Spomenka Z. and Jankov, Ratko M.",
year = "1997",
abstract = "We investigated extracts of timothy grass pollen from four seasons (1989, 1990, 1991, and 1994) by protein content, SDS-PAGE, immunoblot, RAST, RAST inhibition, and crossed immunoelectrophoresis. Extract of the pollen from 1991 showed the lowest yield in quantitative assays. SDS-PAGE, crossed immunoelectrophoresis, RAST, and RAST inhibition expressed approximately comparable patterns for all extracts except that from 1991. Obviously, the quality of grass pollens, as shown for some ragweed (Ambrosia elatior) pollens depend on year of collection. Our findings are partially in agreement with some earlier examinations of the quality of timothy pollen from different pollen seasons.",
journal = "Allergy",
title = "Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area",
volume = "52",
number = "2",
pages = "210-214",
doi = "10.1111/j.1398-9995.1997.tb00977.x"
}
Gavrović, M., Trtić, T. M., Vujčić, Z., Petrović, S. Z.,& Jankov, R. M.. (1997). Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area. in Allergy, 52(2), 210-214.
https://doi.org/10.1111/j.1398-9995.1997.tb00977.x
Gavrović M, Trtić TM, Vujčić Z, Petrović SZ, Jankov RM. Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area. in Allergy. 1997;52(2):210-214.
doi:10.1111/j.1398-9995.1997.tb00977.x .
Gavrović, Marija, Trtić, Tatjana M., Vujčić, Zoran, Petrović, Spomenka Z., Jankov, Ratko M., "Comparison of allergenic potentials of timothy (Phleum pratense) pollens from different pollen seasons collected in the Belgrade area" in Allergy, 52, no. 2 (1997):210-214,
https://doi.org/10.1111/j.1398-9995.1997.tb00977.x . .
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Development of RAST assay for determination of anti-Populus canadensis IgE antibodies

Trtić, Tatjana M.; Vuksanovic, L; Paranos, S; Petrovic, S; Vujčić, Zoran; Plećaš, Ilija B.; Jankov, Ratko M.

(1996)

TY  - JOUR
AU  - Trtić, Tatjana M.
AU  - Vuksanovic, L
AU  - Paranos, S
AU  - Petrovic, S
AU  - Vujčić, Zoran
AU  - Plećaš, Ilija B.
AU  - Jankov, Ratko M.
PY  - 1996
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/7219
AB  - One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.
T2  - Journal of Radioanalytical and Nuclear Chemistry
T1  - Development of RAST assay for determination of anti-Populus canadensis IgE antibodies
VL  - 206
IS  - 1
SP  - 145
EP  - 149
DO  - 10.1007/BF02040051
ER  - 
@article{
author = "Trtić, Tatjana M. and Vuksanovic, L and Paranos, S and Petrovic, S and Vujčić, Zoran and Plećaš, Ilija B. and Jankov, Ratko M.",
year = "1996",
abstract = "One of the frequent causes of pollen allergy in our region (Serbia, Yugoslavia) is the pollen of poplar (Populus canadensis). The aim of this study was to form RAST for the determination of specific anti-Populus canadensis IgE antibodies. Affinity purified and radiolabelled (I-125) MoAb El was used for forming assay for the determination of specific IgE. By titration of extract of poplar Populus canadensis we determined that the quantity of 0.65 mt extract is needed for coupling of ig BrCN activated paper discs. Coupling was performed in Na(2)CO3/NaHCO3 buffer pH 10. on 4 degrees C for 48h. Using this newely formed RAST, specific for Populus canadensis we have deteminated anti- Populus canadensis IgE antibodies as well. as cross reactivity between pollens of Populus canadensis and Populus deltoides.",
journal = "Journal of Radioanalytical and Nuclear Chemistry",
title = "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies",
volume = "206",
number = "1",
pages = "145-149",
doi = "10.1007/BF02040051"
}
Trtić, T. M., Vuksanovic, L., Paranos, S., Petrovic, S., Vujčić, Z., Plećaš, I. B.,& Jankov, R. M.. (1996). Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry, 206(1), 145-149.
https://doi.org/10.1007/BF02040051
Trtić TM, Vuksanovic L, Paranos S, Petrovic S, Vujčić Z, Plećaš IB, Jankov RM. Development of RAST assay for determination of anti-Populus canadensis IgE antibodies. in Journal of Radioanalytical and Nuclear Chemistry. 1996;206(1):145-149.
doi:10.1007/BF02040051 .
Trtić, Tatjana M., Vuksanovic, L, Paranos, S, Petrovic, S, Vujčić, Zoran, Plećaš, Ilija B., Jankov, Ratko M., "Development of RAST assay for determination of anti-Populus canadensis IgE antibodies" in Journal of Radioanalytical and Nuclear Chemistry, 206, no. 1 (1996):145-149,
https://doi.org/10.1007/BF02040051 . .

Temperature-programmed desorption mass spectrometric study of the surface properties of glassy carbon

Perić, Aleksandra A.; Nešković, Olivera M.; Veljković, Miomir V.; Miletić, Miodrag B.; Zmbov, K; Petrović, S; Laušević, Mila D.; Laušević, Zoran

(1996)

TY  - JOUR
AU  - Perić, Aleksandra A.
AU  - Nešković, Olivera M.
AU  - Veljković, Miomir V.
AU  - Miletić, Miodrag B.
AU  - Zmbov, K
AU  - Petrović, S
AU  - Laušević, Mila D.
AU  - Laušević, Zoran
PY  - 1996
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2004
AB  - The temperature-programmed desorption (TPD) method combined with mass spectrometric analysis has been applied to the characterization of glassy carbon (GC) and modified glassy carbon surfaces. Five different samples of GC were obtained from a commercial resol type of phenol formaldehyde resin that bad undergone several treatments: the samples were either pure GC, GC treated with boron and phosphorus, or GC modified by metal (silver and palladium). Mass spectrometric analysis has shown that the thermal decomposition of surface oxide species results in desorption of H2O, CO and CO2, these being the major gas products. On the basis of the TPD spectra obtained, desorption energies have been calculated using a peak position method. The influence of CO2 oxidation on the surface properties of the samples was also investigated. It was concluded that metal deposition on a glassy carbon surface contributes to increased stability of surface oxide species, the most stable surface being one that has been modified by palladium deposition.
T2  - Rapid Communications in Mass Spectrometry
T1  - Temperature-programmed desorption mass spectrometric study of the surface properties of glassy carbon
VL  - 10
IS  - 10
SP  - 1233
EP  - 1236
DO  - 10.1002/(SICI)1097-0231(19960731)10:10<1233::AID-RCM268>3.0.CO;2-V
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2004
ER  - 
@article{
author = "Perić, Aleksandra A. and Nešković, Olivera M. and Veljković, Miomir V. and Miletić, Miodrag B. and Zmbov, K and Petrović, S and Laušević, Mila D. and Laušević, Zoran",
year = "1996",
abstract = "The temperature-programmed desorption (TPD) method combined with mass spectrometric analysis has been applied to the characterization of glassy carbon (GC) and modified glassy carbon surfaces. Five different samples of GC were obtained from a commercial resol type of phenol formaldehyde resin that bad undergone several treatments: the samples were either pure GC, GC treated with boron and phosphorus, or GC modified by metal (silver and palladium). Mass spectrometric analysis has shown that the thermal decomposition of surface oxide species results in desorption of H2O, CO and CO2, these being the major gas products. On the basis of the TPD spectra obtained, desorption energies have been calculated using a peak position method. The influence of CO2 oxidation on the surface properties of the samples was also investigated. It was concluded that metal deposition on a glassy carbon surface contributes to increased stability of surface oxide species, the most stable surface being one that has been modified by palladium deposition.",
journal = "Rapid Communications in Mass Spectrometry",
title = "Temperature-programmed desorption mass spectrometric study of the surface properties of glassy carbon",
volume = "10",
number = "10",
pages = "1233-1236",
doi = "10.1002/(SICI)1097-0231(19960731)10:10<1233::AID-RCM268>3.0.CO;2-V",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2004"
}
Perić, A. A., Nešković, O. M., Veljković, M. V., Miletić, M. B., Zmbov, K., Petrović, S., Laušević, M. D.,& Laušević, Z.. (1996). Temperature-programmed desorption mass spectrometric study of the surface properties of glassy carbon. in Rapid Communications in Mass Spectrometry, 10(10), 1233-1236.
https://doi.org/10.1002/(SICI)1097-0231(19960731)10:10<1233::AID-RCM268>3.0.CO;2-V
https://hdl.handle.net/21.15107/rcub_vinar_2004
Perić AA, Nešković OM, Veljković MV, Miletić MB, Zmbov K, Petrović S, Laušević MD, Laušević Z. Temperature-programmed desorption mass spectrometric study of the surface properties of glassy carbon. in Rapid Communications in Mass Spectrometry. 1996;10(10):1233-1236.
doi:10.1002/(SICI)1097-0231(19960731)10:10<1233::AID-RCM268>3.0.CO;2-V
https://hdl.handle.net/21.15107/rcub_vinar_2004 .
Perić, Aleksandra A., Nešković, Olivera M., Veljković, Miomir V., Miletić, Miodrag B., Zmbov, K, Petrović, S, Laušević, Mila D., Laušević, Zoran, "Temperature-programmed desorption mass spectrometric study of the surface properties of glassy carbon" in Rapid Communications in Mass Spectrometry, 10, no. 10 (1996):1233-1236,
https://doi.org/10.1002/(SICI)1097-0231(19960731)10:10<1233::AID-RCM268>3.0.CO;2-V .,
https://hdl.handle.net/21.15107/rcub_vinar_2004 .
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