TY  - CONF
AU  - Kolaković, Ana P.
AU  - Živković, Maja
AU  - Dinčić, Evica
AU  - Popović, Smiljana
AU  - Raičević, Ranko
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2010
PY  - 10 Supplement
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12791
AB  - Multiple  sclerosis  (MS)  is  a  complex  inflammatory,demyelinating disease of central nervous system (CNS). All the essen-tial components of the renin-angiotensin system (RAS) are presentedin  the  mammalian  brain.  The  angiotensin  II  (Ang  II),  biologicallyactive octapeptide is not only a vasoconstrictor, but also a pro-inflam-matory factor. Many of the classical and of the hypothetical functionsof brain Ang II are mediated by stimulation of AT1 receptors (AT1R).Brain AT2 receptors (AT2R) are highly expressed during development.In the adults, AT2R are restricted to areas predominantly involved inthe process of sensory information. The AT2R1332 A/G polymorph-ism was proposed to influence AT2R protein expression, and is themost studied polymorphism in this gene, in other diseases. Recently,the  striking  appearance  of  the  RAS  in  MS  brain  was  described.However, the role of AT2R remains to be clarified. Thus, the aim ofour  study  was  to  establish  if  there  is  an  association  between  AT2R1332 A/G gene polymorphism and predisposition of MS Methods:Subjected  group  consisted  of  122  female  and  70  malepatients with MS and 75 female and 50 male controls from populationof Serbia. Genotyping was done by PCR and restriction digestion withEcoRI enzyme.Results:The  genotype  and  allele  frequencies  for  AT2R1332A/Ggene  polymorphism  are  analyzed  separately  in  females  and  males,since this gene is located on X chromosome. We detected significantoverrepresentation of1332A/G AA genotype (OR 1.6, 95% CI:1.0–2.7, p<0.05) in female patients with MS compared to female controls.In hemizygous males we didn’t found any difference between patientsand controls.Conclusion:The  role  of  RAS  genes  in  MS  was  neglected  untilrecently.  Than,  it  was  shown  that  the  role  of  RAS  in  the  CNS  isbeyond  the  regulation  of  cardiovascular  function.  Until  now  AT2R(1332A/G)  gene  polymorphism  was widely studied  and associatedwith hypertension and other vascular disease. Until now, there wereno studies concerning role of Ang II receptor polymorphisms in MS.This study  suggest  possible  role  of  AT2R in  MS.  Further  studies areneeded to elucidate this result.
C3  - Multiple Sclerosis Journal
T1  - Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis
VL  - 16
SP  - S80
EP  - S81
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12791
ER  - 

@conference{
author = "Kolaković, Ana P. and Živković, Maja and Dinčić, Evica and Popović, Smiljana and Raičević, Ranko and Alavantić, Dragan and Stanković, Aleksandra",
year = "2010, 10 Supplement",
abstract = "Multiple  sclerosis  (MS)  is  a  complex  inflammatory,demyelinating disease of central nervous system (CNS). All the essen-tial components of the renin-angiotensin system (RAS) are presentedin  the  mammalian  brain.  The  angiotensin  II  (Ang  II),  biologicallyactive octapeptide is not only a vasoconstrictor, but also a pro-inflam-matory factor. Many of the classical and of the hypothetical functionsof brain Ang II are mediated by stimulation of AT1 receptors (AT1R).Brain AT2 receptors (AT2R) are highly expressed during development.In the adults, AT2R are restricted to areas predominantly involved inthe process of sensory information. The AT2R1332 A/G polymorph-ism was proposed to influence AT2R protein expression, and is themost studied polymorphism in this gene, in other diseases. Recently,the  striking  appearance  of  the  RAS  in  MS  brain  was  described.However, the role of AT2R remains to be clarified. Thus, the aim ofour  study  was  to  establish  if  there  is  an  association  between  AT2R1332 A/G gene polymorphism and predisposition of MS Methods:Subjected  group  consisted  of  122  female  and  70  malepatients with MS and 75 female and 50 male controls from populationof Serbia. Genotyping was done by PCR and restriction digestion withEcoRI enzyme.Results:The  genotype  and  allele  frequencies  for  AT2R1332A/Ggene  polymorphism  are  analyzed  separately  in  females  and  males,since this gene is located on X chromosome. We detected significantoverrepresentation of1332A/G AA genotype (OR 1.6, 95% CI:1.0–2.7, p<0.05) in female patients with MS compared to female controls.In hemizygous males we didn’t found any difference between patientsand controls.Conclusion:The  role  of  RAS  genes  in  MS  was  neglected  untilrecently.  Than,  it  was  shown  that  the  role  of  RAS  in  the  CNS  isbeyond  the  regulation  of  cardiovascular  function.  Until  now  AT2R(1332A/G)  gene  polymorphism  was widely studied  and associatedwith hypertension and other vascular disease. Until now, there wereno studies concerning role of Ang II receptor polymorphisms in MS.This study  suggest  possible  role  of  AT2R in  MS.  Further  studies areneeded to elucidate this result.",
journal = "Multiple Sclerosis Journal",
title = "Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis",
volume = "16",
pages = "S80-S81",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12791"
}

Kolaković, A. P., Živković, M., Dinčić, E., Popović, S., Raičević, R., Alavantić, D.,& Stanković, A.. (2010). Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis. in Multiple Sclerosis Journal, 16, S80-S81.
https://hdl.handle.net/21.15107/rcub_vinar_12791

Kolaković AP, Živković M, Dinčić E, Popović S, Raičević R, Alavantić D, Stanković A. Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis. in Multiple Sclerosis Journal. 2010;16:S80-S81.
https://hdl.handle.net/21.15107/rcub_vinar_12791 .

Kolaković, Ana P., Živković, Maja, Dinčić, Evica, Popović, Smiljana, Raičević, Ranko, Alavantić, Dragan, Stanković, Aleksandra, "Angiotensin II receptor type 2 (AT2R) -1332 A/G gene polymorphism as a risk factor for multiple sclerosis" in Multiple Sclerosis Journal, 16 (2010):S80-S81,
https://hdl.handle.net/21.15107/rcub_vinar_12791 .

TY  - JOUR
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Raičević, Ranko
AU  - Alavantić, Dragan
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3237
AB  - Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in remodeling of the extracellular matrix. MMPs are suggested to play a role in the influx of inflammatory cells into the CNS, disruption of the blood brain barrier, and to degrade myelin in vitro. In this study, we have investigated the possible association of MMP-3 5A/6A gene polymorphism with MS susceptibility and/or severity in patients from Serbia. A total of 184 MS patients (150 RR, 34 SP) and 236 controls have been studied. Results show that the distribution of MMP-3 5A/6A genotype frequencies between NIS patients and controls were not significantly different. In bout onset patients, carriers of MMP-3 6A/6A genotype had significantly higher mean MSSS values compared to the carriers of 5A allele (6.29 +/- 1.89 vs. 5.29 +/- 2.62, respectively, ANCOVA, p=0.01 Scheffe post-hoc test). In conclusion, our results indicate association of MMP-3 6A/6A genotype with significantly higher mean MSSS values. Thus, the obtained results suggest that it should be carefully considered during follow up of patients with MS. Further genetic and functional studies are needed to resolve the complex role of MMPs and their tissue inhibitors in NIS pathology and/or regeneration. (C) 2007 Elsevier B.V. All rights reserved.
T2  - Journal of the Neurological Sciences
T1  - Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia
VL  - 267
IS  - 1-2
SP  - 62
EP  - 65
DO  - 10.1016/j.jns.2007.09.037
ER  - 

@article{
author = "Đurić, Tamara and Živković, Maja and Stanković, Aleksandra and Dinčić, Evica and Raičević, Ranko and Alavantić, Dragan",
year = "2008",
abstract = "Matrix metalloproteinases (MMPs) are proteolytic enzymes involved in remodeling of the extracellular matrix. MMPs are suggested to play a role in the influx of inflammatory cells into the CNS, disruption of the blood brain barrier, and to degrade myelin in vitro. In this study, we have investigated the possible association of MMP-3 5A/6A gene polymorphism with MS susceptibility and/or severity in patients from Serbia. A total of 184 MS patients (150 RR, 34 SP) and 236 controls have been studied. Results show that the distribution of MMP-3 5A/6A genotype frequencies between NIS patients and controls were not significantly different. In bout onset patients, carriers of MMP-3 6A/6A genotype had significantly higher mean MSSS values compared to the carriers of 5A allele (6.29 +/- 1.89 vs. 5.29 +/- 2.62, respectively, ANCOVA, p=0.01 Scheffe post-hoc test). In conclusion, our results indicate association of MMP-3 6A/6A genotype with significantly higher mean MSSS values. Thus, the obtained results suggest that it should be carefully considered during follow up of patients with MS. Further genetic and functional studies are needed to resolve the complex role of MMPs and their tissue inhibitors in NIS pathology and/or regeneration. (C) 2007 Elsevier B.V. All rights reserved.",
journal = "Journal of the Neurological Sciences",
title = "Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia",
volume = "267",
number = "1-2",
pages = "62-65",
doi = "10.1016/j.jns.2007.09.037"
}

Đurić, T., Živković, M., Stanković, A., Dinčić, E., Raičević, R.,& Alavantić, D.. (2008). Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia. in Journal of the Neurological Sciences, 267(1-2), 62-65.
https://doi.org/10.1016/j.jns.2007.09.037

Đurić T, Živković M, Stanković A, Dinčić E, Raičević R, Alavantić D. Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia. in Journal of the Neurological Sciences. 2008;267(1-2):62-65.
doi:10.1016/j.jns.2007.09.037 .

Đurić, Tamara, Živković, Maja, Stanković, Aleksandra, Dinčić, Evica, Raičević, Ranko, Alavantić, Dragan, "Association of the MMP-3 5A/6A gene polymorphism with multiple sclerosis in patients from Serbia" in Journal of the Neurological Sciences, 267, no. 1-2 (2008):62-65,
https://doi.org/10.1016/j.jns.2007.09.037 . .