TY  - JOUR
AU  - Đurić, Marko
AU  - Mutavdžin, Slavica
AU  - Lončar-Stojiljković, Dragana
AU  - Kostić, Sanja
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Živković, Vladimir
AU  - Jakovljević, Vladimir
AU  - Đurić, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12972
AB  - Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.
T2  - Scripta Medica
T1  - The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity
VL  - 50
IS  - 3
SP  - 112
EP  - 116
DO  - 10.5937/scriptamed50-22658
ER  - 

@article{
author = "Đurić, Marko and Mutavdžin, Slavica and Lončar-Stojiljković, Dragana and Kostić, Sanja and Čolović, Mirjana and Krstić, Danijela and Živković, Vladimir and Jakovljević, Vladimir and Đurić, Dragan",
year = "2019",
abstract = "Background/Aim: Hyperhomocysteinaemia is linked to higher level of acetylcholinesterase (AChE) in brain, but there is insufficient information on influence of homocysteine (Hcy) and gasotransmitters on cardiac AChE. Thus, the aim of this study was to evaluate the influence of certain gasotransmitter inhibitors in Hcy-induced changes on rat cardiac AChE activity. Methods: Research was performed on 72 male Wistar albino rats distributed into 6 groups: 1) Control group – saline (1 ml 0.9 % NaCl ip); 2) DL-Hcy (8 mmol/kg ip DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg ip Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) DL-PAG (50 mg/kg ip DL-propargylglycine (DL-PAG), inhibitor of H2S production); 5) DL-Hcy+L-NAME (8 mmol/ kg ip DL-Hcy + 10 mg/kg ip L-NAME); and 6) DL-Hcy+DL-PAG (8 mmol/kg ip DL-Hcy + 50 mg/kg ip DL-PAG). All tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals’ sacrifice. AChE activity was measured in the rats’ cardiac tissue homogenate. Results: Administration of Hcy and L-NAME induced significant decrease in AChE activity compared with control condition. Administration of DL-PAG, DL-Hcy+LNAME and DL-Hcy+DL-PAG did not change AChE activity compared with the control group. Conclusion: The effects of acute Hcy administration on the cardiac AChE activity are partially mediated via interaction with tested gasotransmitters.",
journal = "Scripta Medica",
title = "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity",
volume = "50",
number = "3",
pages = "112-116",
doi = "10.5937/scriptamed50-22658"
}

Đurić, M., Mutavdžin, S., Lončar-Stojiljković, D., Kostić, S., Čolović, M., Krstić, D., Živković, V., Jakovljević, V.,& Đurić, D.. (2019). The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica, 50(3), 112-116.
https://doi.org/10.5937/scriptamed50-22658

Đurić M, Mutavdžin S, Lončar-Stojiljković D, Kostić S, Čolović M, Krstić D, Živković V, Jakovljević V, Đurić D. The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity. in Scripta Medica. 2019;50(3):112-116.
doi:10.5937/scriptamed50-22658 .

Đurić, Marko, Mutavdžin, Slavica, Lončar-Stojiljković, Dragana, Kostić, Sanja, Čolović, Mirjana, Krstić, Danijela, Živković, Vladimir, Jakovljević, Vladimir, Đurić, Dragan, "The effects of certain gasotransmitters inhibition on homocysteine acutely induced changes on rat cardiac acetylcholinesterase activity" in Scripta Medica, 50, no. 3 (2019):112-116,
https://doi.org/10.5937/scriptamed50-22658 . .

TY  - JOUR
AU  - Đurić, Marko
AU  - Kostić, Sanja
AU  - Lončar-Stojiljković, Dragana
AU  - Mutavdžin, Slavica
AU  - Čolović, Mirjana
AU  - Krstić, Danijela
AU  - Stevanović, Predrag
AU  - Đurić, Dragan
PY  - 2019
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12973
AB  - Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats. Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group - saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured. Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence. Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters.
T2  - Scripta Medica
T1  - The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma
VL  - 50
IS  - 1
SP  - 6
EP  - 12
DO  - 10.5937/scriptamed50-21100
ER  - 

@article{
author = "Đurić, Marko and Kostić, Sanja and Lončar-Stojiljković, Dragana and Mutavdžin, Slavica and Čolović, Mirjana and Krstić, Danijela and Stevanović, Predrag and Đurić, Dragan",
year = "2019",
abstract = "Background: The importance of homocysteine (Hcy) is increasingly recognized in last few decades as an independent risk factor for atherosclerosis and thrombosis, but there is lack of data referring to influence of Hcy on plasma oxidative stress parameters as well as the role of gasotransmitters in these effects. Therefore, this study aim was to assess the role of gasotransmitter inhibitors in Hcy-induced effects on plasma oxidative stress in rats. Material and Methods: Study involved 96 male Wistar albino rats divided into 8 groups: 1) Control group - saline (1ml 0.9 % NaCl i.p.); 2) DL-Hcy (8 mmol/kg i.p. DL homocysteine (DL-Hcy); 3) L-NAME (10 mg/kg i.p. Nω-Nitro-L-arginine methyl ester (L-NAME), inhibitor of NO production); 4) ZnPPR IX (30 mol/kg i.p. protoporphyrin IX zinc (ZnPPR IX), inhibitor of CO production); 5) DL-PAG (50 mg//kg i.p. DL-propargylglycine (DL-PAG), inhibitor of H2S production); 6) DL-Hcy+L-NAME (8 mmol/kg i.p. DL-Hcy + 10 mg/kg i.p. L-NAME); 7) DL-Hcy+ZnPPR IX (8 mmol/kg i.p. DL-Hcy + 30 mol/kg i.p. Zn PPR IX), and 8) DL-Hcy+DL-PAG (8 mmol/kg i.p. DL-Hcy + 50 mg//kg i.p. DL-PAG). In all experimental groups, tested substances were administered in a single dose, intraperitoneally, 60 minutes before animals' euthanasia. In the collected blood samples malondialdehyde concentration, catalase, glutathione peroxidase and superoxide dismutase activity were measured. Results: Applied substances induced rapid and strong increase of plasma antioxidant enzymatic activity probably as a compensatory response to its pro-oxidant influence. Conclusion: The effects of Hcy on the activity of plasma antioxidant enzymes are in part mediated via interaction with gasotransmitters.",
journal = "Scripta Medica",
title = "The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma",
volume = "50",
number = "1",
pages = "6-12",
doi = "10.5937/scriptamed50-21100"
}

Đurić, M., Kostić, S., Lončar-Stojiljković, D., Mutavdžin, S., Čolović, M., Krstić, D., Stevanović, P.,& Đurić, D.. (2019). The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma. in Scripta Medica, 50(1), 6-12.
https://doi.org/10.5937/scriptamed50-21100

Đurić M, Kostić S, Lončar-Stojiljković D, Mutavdžin S, Čolović M, Krstić D, Stevanović P, Đurić D. The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma. in Scripta Medica. 2019;50(1):6-12.
doi:10.5937/scriptamed50-21100 .

Đurić, Marko, Kostić, Sanja, Lončar-Stojiljković, Dragana, Mutavdžin, Slavica, Čolović, Mirjana, Krstić, Danijela, Stevanović, Predrag, Đurić, Dragan, "The effects of gasotransmitters inhibition on homocysteine acutely induced changes in oxidative stress markers in rat plasma" in Scripta Medica, 50, no. 1 (2019):6-12,
https://doi.org/10.5937/scriptamed50-21100 . .