TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Gemović, Branislava
AU  - Senćanski, Milan
AU  - Algarra, Manuel
AU  - Gonçalves, Mara
AU  - Stepić, Milutin
AU  - Popović, Iva
AU  - Kapuran, Đorđe
AU  - Petković, Marijana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13575
AB  - We predicted the protein therapeutic targets specific to a Ru-based potential drug and its combination with pristine and N-doped carbon dot drug delivery systems, denoted as RuCN/CDs and RuCN/N-CDs. Synchrotron-based FTIR microspectroscopy (µFTIR) in addition to bioinformatics data on drug structures and protein sequences were applied to assess changes in the protein secondary structure of A2780 cancer cells. µFTIR revealed the moieties of the target proteins’ secondary structure changes only after the treatment with RuCN and RuCN/N-CDs. A higher content of α-helices and a lower content of β-sheets appeared in A2780 cells after RuCN treatment. Treatment with RuCN/N-CDs caused a substantial increase in parallel β-sheet numbers, random coil content, and tyrosine residue numbers. The results obtained suggest that the mitochondrion-related proteins NDUFA1 and NDUFB5 are affected by RuCN either via overexpression or stabilisation of helical structures. RuCN/N-CDs either induce overexpression of the β-sheet-rich protein NDUFS1 and affect its random coil structure or interact and stabilise its structure via hydrogen bonding between -NH2 groups from N-CDs with protein C=O groups and –OH groups of serine, threonine, and tyrosine residues. The N-CD nanocarrier tunes this drug’s action by directing it toward a specific protein target, changing this drug’s coordination ability and inducing changes in the protein’s secondary structures and function.
T2  - Pharmaceutics
T1  - Prediction of Protein Targets in Ovarian Cancer Using a Ru-Complex and Carbon Dot Drug Delivery Therapeutic Nanosystems: A Bioinformatics and μ-FTIR Spectroscopy Approach
VL  - 16
IS  - 8
SP  - 997
DO  - 10.3390/pharmaceutics16080997
ER  - 

@article{
author = "Nešić, Maja D. and Dučić, Tanja and Gemović, Branislava and Senćanski, Milan and Algarra, Manuel and Gonçalves, Mara and Stepić, Milutin and Popović, Iva and Kapuran, Đorđe and Petković, Marijana",
year = "2024",
abstract = "We predicted the protein therapeutic targets specific to a Ru-based potential drug and its combination with pristine and N-doped carbon dot drug delivery systems, denoted as RuCN/CDs and RuCN/N-CDs. Synchrotron-based FTIR microspectroscopy (µFTIR) in addition to bioinformatics data on drug structures and protein sequences were applied to assess changes in the protein secondary structure of A2780 cancer cells. µFTIR revealed the moieties of the target proteins’ secondary structure changes only after the treatment with RuCN and RuCN/N-CDs. A higher content of α-helices and a lower content of β-sheets appeared in A2780 cells after RuCN treatment. Treatment with RuCN/N-CDs caused a substantial increase in parallel β-sheet numbers, random coil content, and tyrosine residue numbers. The results obtained suggest that the mitochondrion-related proteins NDUFA1 and NDUFB5 are affected by RuCN either via overexpression or stabilisation of helical structures. RuCN/N-CDs either induce overexpression of the β-sheet-rich protein NDUFS1 and affect its random coil structure or interact and stabilise its structure via hydrogen bonding between -NH2 groups from N-CDs with protein C=O groups and –OH groups of serine, threonine, and tyrosine residues. The N-CD nanocarrier tunes this drug’s action by directing it toward a specific protein target, changing this drug’s coordination ability and inducing changes in the protein’s secondary structures and function.",
journal = "Pharmaceutics",
title = "Prediction of Protein Targets in Ovarian Cancer Using a Ru-Complex and Carbon Dot Drug Delivery Therapeutic Nanosystems: A Bioinformatics and μ-FTIR Spectroscopy Approach",
volume = "16",
number = "8",
pages = "997",
doi = "10.3390/pharmaceutics16080997"
}

Nešić, M. D., Dučić, T., Gemović, B., Senćanski, M., Algarra, M., Gonçalves, M., Stepić, M., Popović, I., Kapuran, Đ.,& Petković, M.. (2024). Prediction of Protein Targets in Ovarian Cancer Using a Ru-Complex and Carbon Dot Drug Delivery Therapeutic Nanosystems: A Bioinformatics and μ-FTIR Spectroscopy Approach. in Pharmaceutics, 16(8), 997.
https://doi.org/10.3390/pharmaceutics16080997

Nešić MD, Dučić T, Gemović B, Senćanski M, Algarra M, Gonçalves M, Stepić M, Popović I, Kapuran Đ, Petković M. Prediction of Protein Targets in Ovarian Cancer Using a Ru-Complex and Carbon Dot Drug Delivery Therapeutic Nanosystems: A Bioinformatics and μ-FTIR Spectroscopy Approach. in Pharmaceutics. 2024;16(8):997.
doi:10.3390/pharmaceutics16080997 .

Nešić, Maja D., Dučić, Tanja, Gemović, Branislava, Senćanski, Milan, Algarra, Manuel, Gonçalves, Mara, Stepić, Milutin, Popović, Iva, Kapuran, Đorđe, Petković, Marijana, "Prediction of Protein Targets in Ovarian Cancer Using a Ru-Complex and Carbon Dot Drug Delivery Therapeutic Nanosystems: A Bioinformatics and μ-FTIR Spectroscopy Approach" in Pharmaceutics, 16, no. 8 (2024):997,
https://doi.org/10.3390/pharmaceutics16080997 . .

TY  - JOUR
AU  - Rackov, Sanja
AU  - Pilić, Branka
AU  - Janković, Nenad
AU  - Kosanić, Marijana
AU  - Petković, Marijana
AU  - Vraneš, Milan
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13670
AB  - Herein, we report an efficient and facile strategy for the preparation of imidazolium-based ionic liquid (IL) monomers ([CnVIm][Br], n = 2, 4, 6, 8, 10, and 12) and their corresponding polymeric ionic liquids (PILs) with potent antimicrobial activities against Gram-negative and Gram-positive bacteria and fungi. The electrospinning technique was utilized to tailor the polymers with the highest antimicrobial potency into porous membranes that can be easily implemented into diverse systems and extend their practical bactericidal application. The antimicrobial mechanism of obtained ILs, polymers, and nanomaterials is considered concerning the bearing chain length, polymerization process, and applied processing technique that provides a unique fibrous structure. The structure composition was selected due to the well-established inherent amphiphilicity that 1-alkylimidazolium ILs possess, coupled with proven antimicrobial, antiseptic, and antifungal behavior. The customizable nature of ILs and PILs complemented with electrospinning is exploited for the development of innovative antimicrobial performances born from the intrinsic polymer itself, offering solutions to the increasing challenge of bacterial resistance. This study opens up new prospects toward designer membranes providing a complete route in their designing and revolutionizing the approach of fabricating multi-functional systems with tunable physicochemical, surface properties, and interesting morphology.
T2  - Polymers
T1  - From Synthesis to Functionality: Tailored Ionic Liquid-Based Electrospun Fibers with Superior Antimicrobial Properties
VL  - 16
IS  - 15
SP  - 2094
DO  - 10.3390/polym16152094
ER  - 

@article{
author = "Rackov, Sanja and Pilić, Branka and Janković, Nenad and Kosanić, Marijana and Petković, Marijana and Vraneš, Milan",
year = "2024",
abstract = "Herein, we report an efficient and facile strategy for the preparation of imidazolium-based ionic liquid (IL) monomers ([CnVIm][Br], n = 2, 4, 6, 8, 10, and 12) and their corresponding polymeric ionic liquids (PILs) with potent antimicrobial activities against Gram-negative and Gram-positive bacteria and fungi. The electrospinning technique was utilized to tailor the polymers with the highest antimicrobial potency into porous membranes that can be easily implemented into diverse systems and extend their practical bactericidal application. The antimicrobial mechanism of obtained ILs, polymers, and nanomaterials is considered concerning the bearing chain length, polymerization process, and applied processing technique that provides a unique fibrous structure. The structure composition was selected due to the well-established inherent amphiphilicity that 1-alkylimidazolium ILs possess, coupled with proven antimicrobial, antiseptic, and antifungal behavior. The customizable nature of ILs and PILs complemented with electrospinning is exploited for the development of innovative antimicrobial performances born from the intrinsic polymer itself, offering solutions to the increasing challenge of bacterial resistance. This study opens up new prospects toward designer membranes providing a complete route in their designing and revolutionizing the approach of fabricating multi-functional systems with tunable physicochemical, surface properties, and interesting morphology.",
journal = "Polymers",
title = "From Synthesis to Functionality: Tailored Ionic Liquid-Based Electrospun Fibers with Superior Antimicrobial Properties",
volume = "16",
number = "15",
pages = "2094",
doi = "10.3390/polym16152094"
}

Rackov, S., Pilić, B., Janković, N., Kosanić, M., Petković, M.,& Vraneš, M.. (2024). From Synthesis to Functionality: Tailored Ionic Liquid-Based Electrospun Fibers with Superior Antimicrobial Properties. in Polymers, 16(15), 2094.
https://doi.org/10.3390/polym16152094

Rackov S, Pilić B, Janković N, Kosanić M, Petković M, Vraneš M. From Synthesis to Functionality: Tailored Ionic Liquid-Based Electrospun Fibers with Superior Antimicrobial Properties. in Polymers. 2024;16(15):2094.
doi:10.3390/polym16152094 .

Rackov, Sanja, Pilić, Branka, Janković, Nenad, Kosanić, Marijana, Petković, Marijana, Vraneš, Milan, "From Synthesis to Functionality: Tailored Ionic Liquid-Based Electrospun Fibers with Superior Antimicrobial Properties" in Polymers, 16, no. 15 (2024):2094,
https://doi.org/10.3390/polym16152094 . .

TY  - JOUR
AU  - Algarra, Manuel
AU  - Carrillo, Celia
AU  - Nešić, Maja D.
AU  - Filipović Tričković, Jelena
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Jiménez-Jiménez, José
AU  - Rodriguez-Castellón, Enrique
AU  - Bandosz, Teresa J.
AU  - Petković, Marijana
AU  - Soto, Juan
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12247
AB  - This paper explains the basis for the excitation energy-independent fluorescence emission of biomass-derived carbon dots (CDs) and shows that these CDs have excellent anti-melanoma and anti-metastatic potential. Additionally, we demonstrate that the black carrots´-derived CDs can be exploited as cell cycle-sensing agents, because of the interaction with chromatin material. Besides their optical properties, fluorescent CDs have gained increased attention for image-guided cancer treatment due to their water solubility, environmental friendliness, affordability, ease of synthesis, and primary biocompatibility. CDs have excellent photostability, determined by their precursors and synthesis pathways. In this study, CDs with chemically homogenous surface functional groups were made using a hydrothermal technique from black carrot extract, an anthocyanin-rich substance derived from biomass. The anti-cancer and anti-metastatic properties of black carrot-derived CDs can be attributed to flavylium cations on the surface, spherical forms, and high water dispersibility. Most importantly, these CDs demonstrate a stable emission at a single wavelength, 612 nm, independent of the excitation energy, which we have explained theoretically for the first time.
T2  - Journal of Molecular Structure
T1  - Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential
VL  - 1300
SP  - 137245
DO  - 10.1016/j.molstruc.2023.137245
ER  - 

@article{
author = "Algarra, Manuel and Carrillo, Celia and Nešić, Maja D. and Filipović Tričković, Jelena and Žakula, Jelena and Korićanac, Lela and Jiménez-Jiménez, José and Rodriguez-Castellón, Enrique and Bandosz, Teresa J. and Petković, Marijana and Soto, Juan",
year = "2024",
abstract = "This paper explains the basis for the excitation energy-independent fluorescence emission of biomass-derived carbon dots (CDs) and shows that these CDs have excellent anti-melanoma and anti-metastatic potential. Additionally, we demonstrate that the black carrots´-derived CDs can be exploited as cell cycle-sensing agents, because of the interaction with chromatin material. Besides their optical properties, fluorescent CDs have gained increased attention for image-guided cancer treatment due to their water solubility, environmental friendliness, affordability, ease of synthesis, and primary biocompatibility. CDs have excellent photostability, determined by their precursors and synthesis pathways. In this study, CDs with chemically homogenous surface functional groups were made using a hydrothermal technique from black carrot extract, an anthocyanin-rich substance derived from biomass. The anti-cancer and anti-metastatic properties of black carrot-derived CDs can be attributed to flavylium cations on the surface, spherical forms, and high water dispersibility. Most importantly, these CDs demonstrate a stable emission at a single wavelength, 612 nm, independent of the excitation energy, which we have explained theoretically for the first time.",
journal = "Journal of Molecular Structure",
title = "Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential",
volume = "1300",
pages = "137245",
doi = "10.1016/j.molstruc.2023.137245"
}

Algarra, M., Carrillo, C., Nešić, M. D., Filipović Tričković, J., Žakula, J., Korićanac, L., Jiménez-Jiménez, J., Rodriguez-Castellón, E., Bandosz, T. J., Petković, M.,& Soto, J.. (2024). Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential. in Journal of Molecular Structure, 1300, 137245.
https://doi.org/10.1016/j.molstruc.2023.137245

Algarra M, Carrillo C, Nešić MD, Filipović Tričković J, Žakula J, Korićanac L, Jiménez-Jiménez J, Rodriguez-Castellón E, Bandosz TJ, Petković M, Soto J. Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential. in Journal of Molecular Structure. 2024;1300:137245.
doi:10.1016/j.molstruc.2023.137245 .

Algarra, Manuel, Carrillo, Celia, Nešić, Maja D., Filipović Tričković, Jelena, Žakula, Jelena, Korićanac, Lela, Jiménez-Jiménez, José, Rodriguez-Castellón, Enrique, Bandosz, Teresa J., Petković, Marijana, Soto, Juan, "Testing of black-carrots-derived fluorescence imaging and anti-metastatic potential" in Journal of Molecular Structure, 1300 (2024):137245,
https://doi.org/10.1016/j.molstruc.2023.137245 . .

TY  - JOUR
AU  - Abu el Rub, Anamarija
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Radoičić, Marija
AU  - Novaković, Mirjana
AU  - Žakula, Jelena
AU  - Ralić, Vanja
AU  - Petković, Marijana
AU  - Stepić, Milutin
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13214
AB  - The increasing incidence rate of pancreatic cancer worldwide, along with high resistance to existing chemotherapies and radiotherapy, demand new approaches for its treatment. Nanoparticle-guided cancer treatment modalities are promising in their anti-cancer efficiency and upgrading potential. We have synthesized and characterized Gd-doped titanium-dioxide nanoparticles (Gd-TiO 2 NPs), which showed good biocompatibility with MIA PaCa-2 and PANC-1 pancreatic cancer cell lines. To assess the effect that these NPs have on tumor cells, we used synchrotron radiation Fourier transform infrared spectroscopy and principal component analysis. We elucidated the biochemical changes in the two cell lines induced after their interaction with these NPs. Results demonstrated changes in nucleic acids and carbohydrate metabolism and alterations in the protein conformation in both cell lines. In addition, considerable changes in lipid area were observed in PANC-1 cell line, suggesting that Gd-TiO 2 NPs have a prospective to be further studied as systems for pancreatic cancer treatment.
T2  - Journal of Molecular Structure
T1  - Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles
VL  - 1309
SP  - 138167
DO  - 10.1016/j.molstruc.2024.138167
ER  - 

@article{
author = "Abu el Rub, Anamarija and Nešić, Maja D. and Dučić, Tanja and Radoičić, Marija and Novaković, Mirjana and Žakula, Jelena and Ralić, Vanja and Petković, Marijana and Stepić, Milutin",
year = "2024",
abstract = "The increasing incidence rate of pancreatic cancer worldwide, along with high resistance to existing chemotherapies and radiotherapy, demand new approaches for its treatment. Nanoparticle-guided cancer treatment modalities are promising in their anti-cancer efficiency and upgrading potential. We have synthesized and characterized Gd-doped titanium-dioxide nanoparticles (Gd-TiO 2 NPs), which showed good biocompatibility with MIA PaCa-2 and PANC-1 pancreatic cancer cell lines. To assess the effect that these NPs have on tumor cells, we used synchrotron radiation Fourier transform infrared spectroscopy and principal component analysis. We elucidated the biochemical changes in the two cell lines induced after their interaction with these NPs. Results demonstrated changes in nucleic acids and carbohydrate metabolism and alterations in the protein conformation in both cell lines. In addition, considerable changes in lipid area were observed in PANC-1 cell line, suggesting that Gd-TiO 2 NPs have a prospective to be further studied as systems for pancreatic cancer treatment.",
journal = "Journal of Molecular Structure",
title = "Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles",
volume = "1309",
pages = "138167",
doi = "10.1016/j.molstruc.2024.138167"
}

Abu el Rub, A., Nešić, M. D., Dučić, T., Radoičić, M., Novaković, M., Žakula, J., Ralić, V., Petković, M.,& Stepić, M.. (2024). Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles. in Journal of Molecular Structure, 1309, 138167.
https://doi.org/10.1016/j.molstruc.2024.138167

Abu el Rub A, Nešić MD, Dučić T, Radoičić M, Novaković M, Žakula J, Ralić V, Petković M, Stepić M. Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles. in Journal of Molecular Structure. 2024;1309:138167.
doi:10.1016/j.molstruc.2024.138167 .

Abu el Rub, Anamarija, Nešić, Maja D., Dučić, Tanja, Radoičić, Marija, Novaković, Mirjana, Žakula, Jelena, Ralić, Vanja, Petković, Marijana, Stepić, Milutin, "Synchrotron radiation-FTIR biomolecular assessment in PANC-1 and MIA PaCa-2 pancreatic cancer cells after treatment with Gd-TiO2 nanoparticles" in Journal of Molecular Structure, 1309 (2024):138167,
https://doi.org/10.1016/j.molstruc.2024.138167 . .

TY  - CONF
AU  - Nešić, Maja D.
AU  - Popović, Iva
AU  - Ralić, Vanja
AU  - Dučić, Tanja
AU  - Algarra, M.
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13564
PB  - Portugal : SPOF - Sociedade Portuguesa para a Investigação e Desenvolvimento em Óptica e Fotónica
C3  - AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts
T1  - Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots
SP  - 49
EP  - 49
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13564
ER  - 

@conference{
author = "Nešić, Maja D. and Popović, Iva and Ralić, Vanja and Dučić, Tanja and Algarra, M. and Stepić, Milutin and Petković, Marijana",
year = "2024",
publisher = "Portugal : SPOF - Sociedade Portuguesa para a Investigação e Desenvolvimento em Óptica e Fotónica",
journal = "AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts",
title = "Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots",
pages = "49-49",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13564"
}

Nešić, M. D., Popović, I., Ralić, V., Dučić, T., Algarra, M., Stepić, M.,& Petković, M.. (2024). Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots. in AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts
Portugal : SPOF - Sociedade Portuguesa para a Investigação e Desenvolvimento em Óptica e Fotónica., 49-49.
https://hdl.handle.net/21.15107/rcub_vinar_13564

Nešić MD, Popović I, Ralić V, Dučić T, Algarra M, Stepić M, Petković M. Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots. in AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts. 2024;:49-49.
https://hdl.handle.net/21.15107/rcub_vinar_13564 .

Nešić, Maja D., Popović, Iva, Ralić, Vanja, Dučić, Tanja, Algarra, M., Stepić, Milutin, Petković, Marijana, "Synchrotron Radiation Fourier Transform Infrared spectroscopical analysis of biomolecules in MRC-5 fibroblast cells treated with biomass-derived carbon dots" in AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts (2024):49-49,
https://hdl.handle.net/21.15107/rcub_vinar_13564 .

TY  - CONF
AU  - Matijević, Milica
AU  - Nešić, Maja D.
AU  - Estrela-Lopis, Irina
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Popović, Iva
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13565
PB  - Portugal : SPOF - Sociedade Portuguesa para a Investigação e Desenvolvimento em Óptica e Fotónica
C3  - AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts
T1  - Ultrafast laser-assisted production of nano-and micro-plastics and their interaction with cells
SP  - 168
EP  - 168
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13565
ER  - 

@conference{
author = "Matijević, Milica and Nešić, Maja D. and Estrela-Lopis, Irina and Petković, Marijana and Stepić, Milutin and Popović, Iva",
year = "2024",
publisher = "Portugal : SPOF - Sociedade Portuguesa para a Investigação e Desenvolvimento em Óptica e Fotónica",
journal = "AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts",
title = "Ultrafast laser-assisted production of nano-and micro-plastics and their interaction with cells",
pages = "168-168",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13565"
}

Matijević, M., Nešić, M. D., Estrela-Lopis, I., Petković, M., Stepić, M.,& Popović, I.. (2024). Ultrafast laser-assisted production of nano-and micro-plastics and their interaction with cells. in AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts
Portugal : SPOF - Sociedade Portuguesa para a Investigação e Desenvolvimento em Óptica e Fotónica., 168-168.
https://hdl.handle.net/21.15107/rcub_vinar_13565

Matijević M, Nešić MD, Estrela-Lopis I, Petković M, Stepić M, Popović I. Ultrafast laser-assisted production of nano-and micro-plastics and their interaction with cells. in AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts. 2024;:168-168.
https://hdl.handle.net/21.15107/rcub_vinar_13565 .

Matijević, Milica, Nešić, Maja D., Estrela-Lopis, Irina, Petković, Marijana, Stepić, Milutin, Popović, Iva, "Ultrafast laser-assisted production of nano-and micro-plastics and their interaction with cells" in AOP 24 : 6th International Conference on Applications of Optics and Photonics : Book of abstracts (2024):168-168,
https://hdl.handle.net/21.15107/rcub_vinar_13565 .

TY  - CONF
AU  - Popović, Iva
AU  - Nišavić, Marija
AU  - Ralić, Vanja
AU  - Petković, Marijana
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
PY  - 2024
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13567
AB  - Chemotherapy is one of the most effective approaches used to treat cancer, a leading
cause of death worldwide.[1] Ruthenium-based complexes have emerged as a powerful
alternative to state-of-the-art platinum chemotherapeutics due to their reduced toxicity,
higher selectivity and anti-metastatic potential.[2] Here, we employ mass spectrometry and
spectroscopic techniques to characterize a novel cis-[Ru(II)(dcbpy)2Cl2] complex and
investigate its interaction with cellular lipids. In addition, the cytotoxic effect and mode of
action on four standard cancer cell lines, namely melanoma A375, cervical cancer HeLa and
pancreas ductal adenocarcinoma MIA PaCa-2 and PANC-1, were elucidated. Our results
show a dose-dependent cytotoxicity of the complex on A375, MIA PaCa-2 and PANC-1.
Further, the Ru(II) complex reduces the clonogenic potential caused by G1 arrest and
increases ROS production in all tested cell lines. Lipid peroxidation is also observed in
HeLa and MIA PaCa-2 cells, possibly indicating ferroptosis induction. Synchrotron
radiation Fourier Transform Infrared Spectroscopy was applied to study the overall
interaction of a Ru-complex with cellular biomolecules, and our results demonstrated a shift
in bands in the lipid fingerprint area of most cell lines, confirming the lipid rearrangement
process induced by the interaction with Ru(II) complex. The complex significantly
increases the percentage of apoptotic A375, HeLa and MIA PaCa-2, while a significant
decrease of p62 protein, a marker for autophagy induction, is detected solely in A375 cells.
The wound healing assay revealed that the Ru(II) complex reduces the metastatic potential
of HeLa and MIA PaCa-2 cells by reducing their migration ability. Overall, our results
indicate the existence of a general mode of action of the tested Ru(II) complex that involves
growth arrest and ROS production, as well as cancer cell line-specific effects. These involve
dual role trough inducing programmed cell death - apoptosis and autophagy as seen in A375
cells, or apoptosis and ferroptosis as indicated by the results obtained on HeLa and MIA
PaCa-2 cells, where anti-metastatic potential was also observed. This work highlights the
multimodal action of Ru(II) complexes and the broadness of their application as potential
chemotherapeutic agents for treating different cancers.[3]
C3  - ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts
T1  - The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines
SP  - P12
UR  - https://hdl.handle.net/21.15107/rcub_vinar_13567
ER  - 

@conference{
author = "Popović, Iva and Nišavić, Marija and Ralić, Vanja and Petković, Marijana and Žakula, Jelena and Korićanac, Lela and Stepić, Milutin and Nešić, Maja D.",
year = "2024",
abstract = "Chemotherapy is one of the most effective approaches used to treat cancer, a leading
cause of death worldwide.[1] Ruthenium-based complexes have emerged as a powerful
alternative to state-of-the-art platinum chemotherapeutics due to their reduced toxicity,
higher selectivity and anti-metastatic potential.[2] Here, we employ mass spectrometry and
spectroscopic techniques to characterize a novel cis-[Ru(II)(dcbpy)2Cl2] complex and
investigate its interaction with cellular lipids. In addition, the cytotoxic effect and mode of
action on four standard cancer cell lines, namely melanoma A375, cervical cancer HeLa and
pancreas ductal adenocarcinoma MIA PaCa-2 and PANC-1, were elucidated. Our results
show a dose-dependent cytotoxicity of the complex on A375, MIA PaCa-2 and PANC-1.
Further, the Ru(II) complex reduces the clonogenic potential caused by G1 arrest and
increases ROS production in all tested cell lines. Lipid peroxidation is also observed in
HeLa and MIA PaCa-2 cells, possibly indicating ferroptosis induction. Synchrotron
radiation Fourier Transform Infrared Spectroscopy was applied to study the overall
interaction of a Ru-complex with cellular biomolecules, and our results demonstrated a shift
in bands in the lipid fingerprint area of most cell lines, confirming the lipid rearrangement
process induced by the interaction with Ru(II) complex. The complex significantly
increases the percentage of apoptotic A375, HeLa and MIA PaCa-2, while a significant
decrease of p62 protein, a marker for autophagy induction, is detected solely in A375 cells.
The wound healing assay revealed that the Ru(II) complex reduces the metastatic potential
of HeLa and MIA PaCa-2 cells by reducing their migration ability. Overall, our results
indicate the existence of a general mode of action of the tested Ru(II) complex that involves
growth arrest and ROS production, as well as cancer cell line-specific effects. These involve
dual role trough inducing programmed cell death - apoptosis and autophagy as seen in A375
cells, or apoptosis and ferroptosis as indicated by the results obtained on HeLa and MIA
PaCa-2 cells, where anti-metastatic potential was also observed. This work highlights the
multimodal action of Ru(II) complexes and the broadness of their application as potential
chemotherapeutic agents for treating different cancers.[3]",
journal = "ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts",
title = "The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines",
pages = "P12",
url = "https://hdl.handle.net/21.15107/rcub_vinar_13567"
}

Popović, I., Nišavić, M., Ralić, V., Petković, M., Žakula, J., Korićanac, L., Stepić, M.,& Nešić, M. D.. (2024). The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines. in ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts, P12.
https://hdl.handle.net/21.15107/rcub_vinar_13567

Popović I, Nišavić M, Ralić V, Petković M, Žakula J, Korićanac L, Stepić M, Nešić MD. The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines. in ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts. 2024;:P12.
https://hdl.handle.net/21.15107/rcub_vinar_13567 .

Popović, Iva, Nišavić, Marija, Ralić, Vanja, Petković, Marijana, Žakula, Jelena, Korićanac, Lela, Stepić, Milutin, Nešić, Maja D., "The interaction of a novel Ru(II) complex with cellular lipids and induction of programed cell death in cancer cell lines" in ICNI-III : 3rd International Conference on Noncovalent Interactions : Book of abstracts (2024):P12,
https://hdl.handle.net/21.15107/rcub_vinar_13567 .

TY  - CONF
AU  - Popović, Iva
AU  - Valenta Šobot, Ana
AU  - Filipović Tričković, Jelena
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Ralić, Vanja
AU  - Abu el Rub, Anamarija
AU  - Algarra, Manuel
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11808
AB  - In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.
PB  - Belgrade : Vinča Institute of Nuclear Sciences
C3  - PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts
T1  - Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing
SP  - 78
EP  - 78
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11808
ER  - 

@conference{
author = "Popović, Iva and Valenta Šobot, Ana and Filipović Tričković, Jelena and Korićanac, Lela and Žakula, Jelena and Ralić, Vanja and Abu el Rub, Anamarija and Algarra, Manuel and Petković, Marijana and Stepić, Milutin and Nešić, Maja D.",
year = "2023",
abstract = "In recent years researchers have developed new strategies to enhance the effectiveness of wound healing by combining nanoparticles and infra red (IR) light. For example, some studies have shown that nanoparticles can be used to enhance the absorption of near-infrared laser (NIR) light by tissues, leading to increased healing rates [1]. The influence of NIR light on proliferation, collagen production, and wound healing was tested on: keratocytes (HaCaT) and fibroblasts (MRC-5) cells that are used as model systems of human skin equivalents that comprise an epidermal and a dermal compartment of skin. Also, these cells were treated with carbon quantum dots/silver-based metal-organic framework composites (Ag-MoFs-NCDs and Ag-MoFs-SCDs), which previously showed high antibacterial activity [2], without and with laser light. Firstly, we have found the most convenient and effective CW laser intensity (16 mW/cm2) and illumination time (3 minutes), which is not too high and short enough to influence human cells' proliferation and metabolism positively. Additional chemical treatment with Ag-MoFs-NCDs and Ag-MoFs-SCDs results in a further increase in human cell viability. Our measurements showed that the proliferation index in laser-illuminated cells and cells treated with Ag-MoFs-SCDs was at the level of the untreated control. Furthermore, Ag-MoFs-SCDs treatment and laser illumination induced a mild, insignificant increase in cellular proliferation. On the other hand, Ag-MoFs-NCDs treatment led to a more pronounced, albeit not significant increase, in cellular proliferation, while Ag-MoFs-NCDs treatment combined with laser illumination significantly increased proliferation. Also, we have detected a mild change in collagen level estimated by hydroxyproline assay, which may indicate a positive outcome of combined laser illumination and treatment, taking into account that after 48 hours, a change in cell's response to the treatment could be noticed. Finally, based on migration assay, we observe a complete wound closure after 48 hours in fibroblast cells treated with Ag-MoFs-NCDs and near-infrared laser light, Fig. 1.",
publisher = "Belgrade : Vinča Institute of Nuclear Sciences",
journal = "PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts",
title = "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing",
pages = "78-78",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11808"
}

Popović, I., Valenta Šobot, A., Filipović Tričković, J., Korićanac, L., Žakula, J., Ralić, V., Abu el Rub, A., Algarra, M., Petković, M., Stepić, M.,& Nešić, M. D.. (2023). Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts
Belgrade : Vinča Institute of Nuclear Sciences., 78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808

Popović I, Valenta Šobot A, Filipović Tričković J, Korićanac L, Žakula J, Ralić V, Abu el Rub A, Algarra M, Petković M, Stepić M, Nešić MD. Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing. in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts. 2023;:78-78.
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

Popović, Iva, Valenta Šobot, Ana, Filipović Tričković, Jelena, Korićanac, Lela, Žakula, Jelena, Ralić, Vanja, Abu el Rub, Anamarija, Algarra, Manuel, Petković, Marijana, Stepić, Milutin, Nešić, Maja D., "Carbon quantum dots/silver based metal organic framework composites in light enhanced wound healing" in PHOTONICA2023 : 9th International School and Conference on Photonics : Book of abstracts (2023):78-78,
https://hdl.handle.net/21.15107/rcub_vinar_11808 .

TY  - CONF
AU  - Ralić, Vanja
AU  - Gemović, Branislava
AU  - Nešić, Maja D.
AU  - Popović, Iva
AU  - Stepić, Milutin
AU  - Petković, Marijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12390
AB  - In this work, we have applied the Informational Spectrum Method (ISM) to discover a potential protein target in the HeLa cervical cancer cell line for [Pd(dach)Cl2] complex. Since Pd complexes are considered an alternative to traditionally used Pt complexes in anti-cancer therapy, it is essential to elucidate the mechanism of their action. A detailed analysis that also involves screening the known protein databases revealed the proteins of the SOSS complex as the most probable [Pd(dach)Cl2] targets. Since this protein maintains genomic stability, this result shows the potential of the Pd(II) complex as an anti-cancer drug.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells
SP  - 296
EP  - 299
DO  - 10.46793/ICCBI23.296R
ER  - 

@conference{
author = "Ralić, Vanja and Gemović, Branislava and Nešić, Maja D. and Popović, Iva and Stepić, Milutin and Petković, Marijana",
year = "2023",
abstract = "In this work, we have applied the Informational Spectrum Method (ISM) to discover a potential protein target in the HeLa cervical cancer cell line for [Pd(dach)Cl2] complex. Since Pd complexes are considered an alternative to traditionally used Pt complexes in anti-cancer therapy, it is essential to elucidate the mechanism of their action. A detailed analysis that also involves screening the known protein databases revealed the proteins of the SOSS complex as the most probable [Pd(dach)Cl2] targets. Since this protein maintains genomic stability, this result shows the potential of the Pd(II) complex as an anti-cancer drug.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells",
pages = "296-299",
doi = "10.46793/ICCBI23.296R"
}

Ralić, V., Gemović, B., Nešić, M. D., Popović, I., Stepić, M.,& Petković, M.. (2023). Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 296-299.
https://doi.org/10.46793/ICCBI23.296R

Ralić V, Gemović B, Nešić MD, Popović I, Stepić M, Petković M. Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:296-299.
doi:10.46793/ICCBI23.296R .

Ralić, Vanja, Gemović, Branislava, Nešić, Maja D., Popović, Iva, Stepić, Milutin, Petković, Marijana, "Identification of protein target molecules for [Pd(dach)Cl2] complex in HeLa cervical carcinoma cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):296-299,
https://doi.org/10.46793/ICCBI23.296R . .

TY  - CONF
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Popović, Iva
AU  - Algarra, Manuel
AU  - Dučić, Tanja
AU  - Abu el Rub, Anamarija
AU  - Ralić, Vanja
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Filipović Tričković, Jelena
AU  - Valenta Šobot, Ana
AU  - Nešić, Maja D.
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13568
AB  - Small, carbon-based nanoparticles, carbon dots (CDs), are investigated as photosensitizers (PSs) for photodynamic therapy of cancers. The results presented here show biochemical changes in cancer cells treated with surface-modified CDs, which are augmented by illumination, indicating a high potential of CDs as selective drug carriers and potent PSs. In addition, we will show that CDs produced from black carrot extract (biomass) can be exploited for image-guided therapy and be cytotoxic for cancer cells, thanks to their fluorescence properties. Finally, since biomass-fabricated CDs interact strongly with nucleic acids, they can be further developed for cell cycle sensing purposes.
PB  - Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy
SP  - 61
EP  - 66
DO  - 10.46793/ICCBI23.061P
ER  - 

@conference{
author = "Petković, Marijana and Stepić, Milutin and Popović, Iva and Algarra, Manuel and Dučić, Tanja and Abu el Rub, Anamarija and Ralić, Vanja and Korićanac, Lela and Žakula, Jelena and Filipović Tričković, Jelena and Valenta Šobot, Ana and Nešić, Maja D.",
year = "2023",
abstract = "Small, carbon-based nanoparticles, carbon dots (CDs), are investigated as photosensitizers (PSs) for photodynamic therapy of cancers. The results presented here show biochemical changes in cancer cells treated with surface-modified CDs, which are augmented by illumination, indicating a high potential of CDs as selective drug carriers and potent PSs. In addition, we will show that CDs produced from black carrot extract (biomass) can be exploited for image-guided therapy and be cytotoxic for cancer cells, thanks to their fluorescence properties. Finally, since biomass-fabricated CDs interact strongly with nucleic acids, they can be further developed for cell cycle sensing purposes.",
publisher = "Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy",
pages = "61-66",
doi = "10.46793/ICCBI23.061P"
}

Petković, M., Stepić, M., Popović, I., Algarra, M., Dučić, T., Abu el Rub, A., Ralić, V., Korićanac, L., Žakula, J., Filipović Tričković, J., Valenta Šobot, A.,& Nešić, M. D.. (2023). Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Institute for Information Technologies, University of Kragujevac., 61-66.
https://doi.org/10.46793/ICCBI23.061P

Petković M, Stepić M, Popović I, Algarra M, Dučić T, Abu el Rub A, Ralić V, Korićanac L, Žakula J, Filipović Tričković J, Valenta Šobot A, Nešić MD. Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:61-66.
doi:10.46793/ICCBI23.061P .

Petković, Marijana, Stepić, Milutin, Popović, Iva, Algarra, Manuel, Dučić, Tanja, Abu el Rub, Anamarija, Ralić, Vanja, Korićanac, Lela, Žakula, Jelena, Filipović Tričković, Jelena, Valenta Šobot, Ana, Nešić, Maja D., "Application of Carbon-Based Nanocomposite Systems as Photosensitizers for Photodynamic Therapy" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):61-66,
https://doi.org/10.46793/ICCBI23.061P . .

TY  - CONF
AU  - Ralić, Vanja
AU  - Nešić, Maja
AU  - Abu el Rub, Anamarija
AU  - Dučić, Tanja
AU  - Gemović, Branislava
AU  - Algarra, Manuel
AU  - Stepić, Milutin
AU  - Korićanac, Lela
AU  - Žakula, Jelena
AU  - Petković, Marijana
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12657
AB  - Nanocomposite system formulated from surface-modified S-doped carbon dot (S-CD) nanoparticle with a potential metallodrug, palladium(II) complex, dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2] (Pd@S-CD), was investigated as a model system for the treatment of cervical carcinoma (HeLa) cells. To examine the intracellular biochemical effects induced by the Pd@S-CD, we used Synchrotron Radiation-based Fourier-transform infrared spectroscopy (SR FTIR). SR FTIR spectroscopy was employed to investigate the alterations in cellular components’ biochemical composition and secondary structure upon exposure to Pd@S-CD. Spectral analysis, complemented by statistical techniques, revealed changes in biomolecules, lipids, proteins, nucleic acids, and carbohydrates caused by the treatment with Pd@CDs. These results and the increased cytotoxicity of the system demonstrate its high anti-cervical cancer therapeutic potential.
PB  - Kragujevac : Institute for Information Technologies, University of Kragujevac
C3  - ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
T1  - SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells
SP  - 467
EP  - 470
DO  - 10.46793/ICCBI23.467R
ER  - 

@conference{
author = "Ralić, Vanja and Nešić, Maja and Abu el Rub, Anamarija and Dučić, Tanja and Gemović, Branislava and Algarra, Manuel and Stepić, Milutin and Korićanac, Lela and Žakula, Jelena and Petković, Marijana",
year = "2023",
abstract = "Nanocomposite system formulated from surface-modified S-doped carbon dot (S-CD) nanoparticle with a potential metallodrug, palladium(II) complex, dichloro(1,2-diaminocyclohexane)palladium(II), [Pd(dach)Cl2] (Pd@S-CD), was investigated as a model system for the treatment of cervical carcinoma (HeLa) cells. To examine the intracellular biochemical effects induced by the Pd@S-CD, we used Synchrotron Radiation-based Fourier-transform infrared spectroscopy (SR FTIR). SR FTIR spectroscopy was employed to investigate the alterations in cellular components’ biochemical composition and secondary structure upon exposure to Pd@S-CD. Spectral analysis, complemented by statistical techniques, revealed changes in biomolecules, lipids, proteins, nucleic acids, and carbohydrates caused by the treatment with Pd@CDs. These results and the increased cytotoxicity of the system demonstrate its high anti-cervical cancer therapeutic potential.",
publisher = "Kragujevac : Institute for Information Technologies, University of Kragujevac",
journal = "ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings",
title = "SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells",
pages = "467-470",
doi = "10.46793/ICCBI23.467R"
}

Ralić, V., Nešić, M., Abu el Rub, A., Dučić, T., Gemović, B., Algarra, M., Stepić, M., Korićanac, L., Žakula, J.,& Petković, M.. (2023). SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings
Kragujevac : Institute for Information Technologies, University of Kragujevac., 467-470.
https://doi.org/10.46793/ICCBI23.467R

Ralić V, Nešić M, Abu el Rub A, Dučić T, Gemović B, Algarra M, Stepić M, Korićanac L, Žakula J, Petković M. SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells. in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings. 2023;:467-470.
doi:10.46793/ICCBI23.467R .

Ralić, Vanja, Nešić, Maja, Abu el Rub, Anamarija, Dučić, Tanja, Gemović, Branislava, Algarra, Manuel, Stepić, Milutin, Korićanac, Lela, Žakula, Jelena, Petković, Marijana, "SR FTIR spectroscopy investigation of Pd@S-CD nanocomposite system effects on biomolecules in cervical carcinoma cells" in ICCBIKG 2023 : 2nd International Conference on Chemo and Bioinformatics : Book of Proceedings (2023):467-470,
https://doi.org/10.46793/ICCBI23.467R . .

TY  - JOUR
AU  - Houdova, Dominika
AU  - Popović, Iva A.
AU  - Dellinger, Thomas
AU  - Nešić, Maja D.
AU  - Petković, Marijana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10135
AB  - The endangered loggerhead sea turtles (Caretta caretta) are a relatively abundant sea turtle species in Madeiran waters, where they encounter various environmental stressors: from natural to anthropogenic. The physiological stress response is increased corticosterone (CS) level in the blood, achieving 2 ng/mL. Although there are various analytical methods for determining the CS concentration in turtle blood, most of them require tedious procedures for the preparation. In this work, we have tested the possibility of using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) for fast screening and detection of CS in blood from a turtle captured at the coast of Madeira Island. MALDI does not require sample derivatization and is suitable for low sample volume. The linearity, precision, and limit of detection and quantification with three organic MALDI matrices were investigated in this study. The signal-to-noise ratio of the CS-derived signal was used as a parameter for quantification. Isotopically labelled corticosterone was added as a correction factor at fixed concentration to achieve better linearity and precision. Our results demonstrate that this method has the potential for quantification of CS in turtle blood. However, the lowest concentration of CS extracted from plasma that was still detectable by MALDI TOF MS was about 0.04 mg/mL, which is about ten times higher than the expected CS concentration in blood.
T2  - International Journal of Mass Spectrometry
T1  - Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles
VL  - 473
SP  - 116796
DO  - 10.1016/j.ijms.2022.116796
ER  - 

@article{
author = "Houdova, Dominika and Popović, Iva A. and Dellinger, Thomas and Nešić, Maja D. and Petković, Marijana",
year = "2022",
abstract = "The endangered loggerhead sea turtles (Caretta caretta) are a relatively abundant sea turtle species in Madeiran waters, where they encounter various environmental stressors: from natural to anthropogenic. The physiological stress response is increased corticosterone (CS) level in the blood, achieving 2 ng/mL. Although there are various analytical methods for determining the CS concentration in turtle blood, most of them require tedious procedures for the preparation. In this work, we have tested the possibility of using matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) for fast screening and detection of CS in blood from a turtle captured at the coast of Madeira Island. MALDI does not require sample derivatization and is suitable for low sample volume. The linearity, precision, and limit of detection and quantification with three organic MALDI matrices were investigated in this study. The signal-to-noise ratio of the CS-derived signal was used as a parameter for quantification. Isotopically labelled corticosterone was added as a correction factor at fixed concentration to achieve better linearity and precision. Our results demonstrate that this method has the potential for quantification of CS in turtle blood. However, the lowest concentration of CS extracted from plasma that was still detectable by MALDI TOF MS was about 0.04 mg/mL, which is about ten times higher than the expected CS concentration in blood.",
journal = "International Journal of Mass Spectrometry",
title = "Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles",
volume = "473",
pages = "116796",
doi = "10.1016/j.ijms.2022.116796"
}

Houdova, D., Popović, I. A., Dellinger, T., Nešić, M. D.,& Petković, M.. (2022). Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles. in International Journal of Mass Spectrometry, 473, 116796.
https://doi.org/10.1016/j.ijms.2022.116796

Houdova D, Popović IA, Dellinger T, Nešić MD, Petković M. Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles. in International Journal of Mass Spectrometry. 2022;473:116796.
doi:10.1016/j.ijms.2022.116796 .

Houdova, Dominika, Popović, Iva A., Dellinger, Thomas, Nešić, Maja D., Petković, Marijana, "Potential of MALDI TOF mass spectrometry for detection and quantification of corticosterone in the blood of loggerhead sea turtles" in International Journal of Mass Spectrometry, 473 (2022):116796,
https://doi.org/10.1016/j.ijms.2022.116796 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Algarra, Manuel
AU  - Popović, Iva A.
AU  - Stepić, Milutin
AU  - Gonçalves, Mara
AU  - Petković, Marijana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10184
AB  - In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(η5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane–water interface region.
T2  - Cancers
T1  - Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study
VL  - 14
IS  - 5
SP  - 1182
DO  - 10.3390/cancers14051182
ER  - 

@article{
author = "Nešić, Maja D. and Dučić, Tanja and Algarra, Manuel and Popović, Iva A. and Stepić, Milutin and Gonçalves, Mara and Petković, Marijana",
year = "2022",
abstract = "In the last decade, targeting membrane lipids in cancer cells has been a promising approach that deserves attention in the field of anticancer drug development. To get a comprehensive understanding of the effect of the drug [Ru(η5-Cp)(PPh3)2CN] (RuCN) on cell lipidic components, we combine complementary analytical approaches, matrix-assisted laser desorption and ionization time-of-flight mass spectrometry (MALDI TOF MS) and synchrotron radiation-based Fourier transform infrared (SR-FTIR) spectroscopy. Techniques are used for screening the effect of potential metallodrug, RuCN, without and with drug carriers (carbon dots (CDs) and nitrogen-doped carbon dots (N-CDs)) on the lipids of the human ovarian cancer cell line A2780. MALDI TOF MS results revealed that the lysis of ovarian cancer membrane lipids is promoted by RuCN and not by drug carriers (CDs and N-CDs). Furthermore, SR-FTIR results strongly suggested that the phospholipids of cancer cells undergo oxidative stress after the treatment with RuCN that was accompanied by the disordering of the fatty acid chains. On the other hand, using (N-)CDs as RuCN nanocarriers prevented the oxidative stress caused by RuCN but did not prevent the disordering of the fatty acid chain packing. Finally, we demonstrated that RuCN and RuCN/(N-)CDs alter the hydration of the membrane surface in the membrane–water interface region.",
journal = "Cancers",
title = "Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study",
volume = "14",
number = "5",
pages = "1182",
doi = "10.3390/cancers14051182"
}

Nešić, M. D., Dučić, T., Algarra, M., Popović, I. A., Stepić, M., Gonçalves, M.,& Petković, M.. (2022). Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study. in Cancers, 14(5), 1182.
https://doi.org/10.3390/cancers14051182

Nešić MD, Dučić T, Algarra M, Popović IA, Stepić M, Gonçalves M, Petković M. Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study. in Cancers. 2022;14(5):1182.
doi:10.3390/cancers14051182 .

Nešić, Maja D., Dučić, Tanja, Algarra, Manuel, Popović, Iva A., Stepić, Milutin, Gonçalves, Mara, Petković, Marijana, "Lipid Status of A2780 Ovarian Cancer Cells after Treatment with Ruthenium Complex Modified with Carbon Dot Nanocarriers: A Multimodal SR-FTIR Spectroscopy and MALDI TOF Mass Spectrometry Study" in Cancers, 14, no. 5 (2022):1182,
https://doi.org/10.3390/cancers14051182 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Dučić, Tanja
AU  - Gonçalves, Mara
AU  - Stepić, Milutin
AU  - Algarra, Manuel
AU  - Soto, Juan
AU  - Gemović, Branislava S.
AU  - Bandosz, Teresa J.
AU  - Petković, Marijana
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10267
AB  - Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respectively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N-CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N-CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination.
T2  - Chemico-Biological Interactions
T1  - Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex
VL  - 360
SP  - 109950
DO  - 10.1016/j.cbi.2022.109950
ER  - 

@article{
author = "Nešić, Maja D. and Dučić, Tanja and Gonçalves, Mara and Stepić, Milutin and Algarra, Manuel and Soto, Juan and Gemović, Branislava S. and Bandosz, Teresa J. and Petković, Marijana",
year = "2022",
abstract = "Carbon dots (CDs) and N-carbon dots (N-CDs) loaded with Ru-complex (CDs@RuCN, N-CDs@RuCN, respectively) were investigated as media imposing biochemical changes induced by UV illumination of ovarian cancer, A2780, and osteosarcoma, CAL72, cells. Synchrotron radiation-based Fourier Transform Infrared Spectroscopy was performed, and the spectra were subjected to a Principal Component Analysis. The CDs@RuCN and N-CDs@RuCN effects on cancer cells were analyzed by the theoretical modelling of the stability of the composite systems and a protein database search. Moreover, a detailed evaluation of surface and optical properties of CDs@RuCN and N-CDs@RuCN was carried out. Results demonstrated selective action of the CDs@RuCN and N-CDs@RuCN-based photodynamic therapy, with N-CDs@RuCN being the most active in inducing changes in A2780 and CDs@RuCN in CAL72 cells. We assume that different surface charges of nanoparticles led to direct interactions of N-CDs@RuCN with a Wnt signalling pathway in A2780 and those of CDs@RuCN with PI3–K/Akt in CAL72 cells and that further biochemical changes occurred upon light illumination.",
journal = "Chemico-Biological Interactions",
title = "Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex",
volume = "360",
pages = "109950",
doi = "10.1016/j.cbi.2022.109950"
}

Nešić, M. D., Dučić, T., Gonçalves, M., Stepić, M., Algarra, M., Soto, J., Gemović, B. S., Bandosz, T. J.,& Petković, M.. (2022). Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex. in Chemico-Biological Interactions, 360, 109950.
https://doi.org/10.1016/j.cbi.2022.109950

Nešić MD, Dučić T, Gonçalves M, Stepić M, Algarra M, Soto J, Gemović BS, Bandosz TJ, Petković M. Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex. in Chemico-Biological Interactions. 2022;360:109950.
doi:10.1016/j.cbi.2022.109950 .

Nešić, Maja D., Dučić, Tanja, Gonçalves, Mara, Stepić, Milutin, Algarra, Manuel, Soto, Juan, Gemović, Branislava S., Bandosz, Teresa J., Petković, Marijana, "Biochemical changes in cancer cells induced by photoactive nanosystem based on carbon dots loaded with Ru-complex" in Chemico-Biological Interactions, 360 (2022):109950,
https://doi.org/10.1016/j.cbi.2022.109950 . .

TY  - CONF
AU  - Čolakov, Katarina
AU  - Nešić, Maja D.
AU  - Matijević, Milica
AU  - Stepić, Milutin
AU  - Petković, Marijana
AU  - Korićanac, Lela
AU  - Žakula, Jelena
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/11011
AB  - Existing therapies for the treatment of pancreatic cancer are insufficiently effective and accompanied by a large number of side effects. Ruthenium complexes have shown promising antitumor properties in the previous studies 1,2 . Thus, in this investigation, anticancer effects of cis-dichlorobis (2,2'-bipyridyl-4,4'dicarboxylic acid)ruthenium(II) (Ru(II) complex) were evaluated using human pancreatic carcinoma cell lines MIA PaCa-2 and PANC-1 in vitro. Cell viability estimated with SRB assay showed significant antitumor activity of Ru(II) complex on MIA PaCa-2 (~55% of control) 48 and 72 h after treatment. On the other hand, PANC-1 cell viability was decreased only 72 h after treatment with the highest concentration of Ru(II) complex (~70% of control). Seven days after the treatment, analysis of cell survival using clonogenic assay showed a significant decrease in cell growth in both cell lines. Ru(II) complex also caused G 1 cell cycle arrest of ~13% in both cell lines. The highest percentage of apoptotic MIA PaCa-2 cells was obtained 48 h after treatment. In addition, the intracellular level of reactive oxygen species (ROS) was significantly increased, whereas cell migration was reduced in both cell lines. Summarized, Ru(II)complex demonstrates antitumor properties mediated by increased oxidative stress and also implies the antimetastatic potential, which deserves further study.
PB  - Belgrade : Faculty of Chemistry : Serbian Biochemical Society
C3  - Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
T1  - ROS-mediated proapoptotic antitumor effects of Ru(II) complex on pancreatic cancer cells
SP  - 155
UR  - https://hdl.handle.net/21.15107/rcub_vinar_11011
ER  - 

@conference{
author = "Čolakov, Katarina and Nešić, Maja D. and Matijević, Milica and Stepić, Milutin and Petković, Marijana and Korićanac, Lela and Žakula, Jelena",
year = "2022",
abstract = "Existing therapies for the treatment of pancreatic cancer are insufficiently effective and accompanied by a large number of side effects. Ruthenium complexes have shown promising antitumor properties in the previous studies 1,2 . Thus, in this investigation, anticancer effects of cis-dichlorobis (2,2'-bipyridyl-4,4'dicarboxylic acid)ruthenium(II) (Ru(II) complex) were evaluated using human pancreatic carcinoma cell lines MIA PaCa-2 and PANC-1 in vitro. Cell viability estimated with SRB assay showed significant antitumor activity of Ru(II) complex on MIA PaCa-2 (~55% of control) 48 and 72 h after treatment. On the other hand, PANC-1 cell viability was decreased only 72 h after treatment with the highest concentration of Ru(II) complex (~70% of control). Seven days after the treatment, analysis of cell survival using clonogenic assay showed a significant decrease in cell growth in both cell lines. Ru(II) complex also caused G 1 cell cycle arrest of ~13% in both cell lines. The highest percentage of apoptotic MIA PaCa-2 cells was obtained 48 h after treatment. In addition, the intracellular level of reactive oxygen species (ROS) was significantly increased, whereas cell migration was reduced in both cell lines. Summarized, Ru(II)complex demonstrates antitumor properties mediated by increased oxidative stress and also implies the antimetastatic potential, which deserves further study.",
publisher = "Belgrade : Faculty of Chemistry : Serbian Biochemical Society",
journal = "Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia",
title = "ROS-mediated proapoptotic antitumor effects of Ru(II) complex on pancreatic cancer cells",
pages = "155",
url = "https://hdl.handle.net/21.15107/rcub_vinar_11011"
}

Čolakov, K., Nešić, M. D., Matijević, M., Stepić, M., Petković, M., Korićanac, L.,& Žakula, J.. (2022). ROS-mediated proapoptotic antitumor effects of Ru(II) complex on pancreatic cancer cells. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia
Belgrade : Faculty of Chemistry : Serbian Biochemical Society., 155.
https://hdl.handle.net/21.15107/rcub_vinar_11011

Čolakov K, Nešić MD, Matijević M, Stepić M, Petković M, Korićanac L, Žakula J. ROS-mediated proapoptotic antitumor effects of Ru(II) complex on pancreatic cancer cells. in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia. 2022;:155.
https://hdl.handle.net/21.15107/rcub_vinar_11011 .

Čolakov, Katarina, Nešić, Maja D., Matijević, Milica, Stepić, Milutin, Petković, Marijana, Korićanac, Lela, Žakula, Jelena, "ROS-mediated proapoptotic antitumor effects of Ru(II) complex on pancreatic cancer cells" in Serbian Biochemical Society : 11th conference - "Amazing Biochemistry" : proceedings ; September 22-23, 2022; Novi Sad, Serbia (2022):155,
https://hdl.handle.net/21.15107/rcub_vinar_11011 .

TY  - JOUR
AU  - Kljajević, Ljiljana M.
AU  - Nenadović, Miloš
AU  - Petković, Marijana
AU  - Bučevac, Dušan
AU  - Pavlović, Vladimir B.
AU  - Mladenović-Nikolić, Nataša
AU  - Nenadović, Snežana B.
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10246
AB  - The effect of zircon (ZrSiO4) on the physico-chemical and mechanical properties of geopolymer/zircon composites was examined in this study. Four geopolymer/zircon composites containing 10, 20, 30 and 40 wt.% zircon were prepared from metakaolin with alkali activators. Characterization of the obtained geopolymers was performed by X-ray diffraction (XRD), Scanning electron microscope (SEM-EDS), Fourier transform infrared spectroscopy (FTIR) and Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF). XRD results did not confirmed the formation of interconnected phases between added zircon, starting aluminum silicates and alkali activators. Compressive strength of prepared geopolymer was examined. The maximum obtained compressive strength of 70.15 MPa was measured in sample containing the smallest fraction of zircon, i.e., 10 wt.%. Addition of larger amount of zircon (20 wt.%) hinders the progress of geopolymerization reaction and consequently decreases compressive strength.
T2  - Science of Sintering
T1  - Physico-chemical and mechanical properties of geopolymer/zircon composites
VL  - 54
IS  - 1
SP  - 11
EP  - 24
DO  - 10.2298/SOS2201011K
ER  - 

@article{
author = "Kljajević, Ljiljana M. and Nenadović, Miloš and Petković, Marijana and Bučevac, Dušan and Pavlović, Vladimir B. and Mladenović-Nikolić, Nataša and Nenadović, Snežana B.",
year = "2022",
abstract = "The effect of zircon (ZrSiO4) on the physico-chemical and mechanical properties of geopolymer/zircon composites was examined in this study. Four geopolymer/zircon composites containing 10, 20, 30 and 40 wt.% zircon were prepared from metakaolin with alkali activators. Characterization of the obtained geopolymers was performed by X-ray diffraction (XRD), Scanning electron microscope (SEM-EDS), Fourier transform infrared spectroscopy (FTIR) and Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF). XRD results did not confirmed the formation of interconnected phases between added zircon, starting aluminum silicates and alkali activators. Compressive strength of prepared geopolymer was examined. The maximum obtained compressive strength of 70.15 MPa was measured in sample containing the smallest fraction of zircon, i.e., 10 wt.%. Addition of larger amount of zircon (20 wt.%) hinders the progress of geopolymerization reaction and consequently decreases compressive strength.",
journal = "Science of Sintering",
title = "Physico-chemical and mechanical properties of geopolymer/zircon composites",
volume = "54",
number = "1",
pages = "11-24",
doi = "10.2298/SOS2201011K"
}

Kljajević, L. M., Nenadović, M., Petković, M., Bučevac, D., Pavlović, V. B., Mladenović-Nikolić, N.,& Nenadović, S. B.. (2022). Physico-chemical and mechanical properties of geopolymer/zircon composites. in Science of Sintering, 54(1), 11-24.
https://doi.org/10.2298/SOS2201011K

Kljajević LM, Nenadović M, Petković M, Bučevac D, Pavlović VB, Mladenović-Nikolić N, Nenadović SB. Physico-chemical and mechanical properties of geopolymer/zircon composites. in Science of Sintering. 2022;54(1):11-24.
doi:10.2298/SOS2201011K .

Kljajević, Ljiljana M., Nenadović, Miloš, Petković, Marijana, Bučevac, Dušan, Pavlović, Vladimir B., Mladenović-Nikolić, Nataša, Nenadović, Snežana B., "Physico-chemical and mechanical properties of geopolymer/zircon composites" in Science of Sintering, 54, no. 1 (2022):11-24,
https://doi.org/10.2298/SOS2201011K . .

TY  - JOUR
AU  - Dučić, Tanja
AU  - Alves, Carla S.
AU  - Vučinić, Željko
AU  - Lázaro-Martínez, Juan M.
AU  - Petković, Marijana
AU  - Soto, Juan
AU  - Mutavdžić, Dragosav
AU  - Valle Martínez de Yuso, M.
AU  - Radotić, Ksenija
AU  - Algarra, Manuel
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10277
AB  - S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives. (S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption, ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition, synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively. Computational chemistry was applied to establish the model for the most stable bond between S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.
T2  - Journal of Colloid and Interface Science
T1  - S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach
VL  - 623
SP  - 226
EP  - 237
DO  - 10.1016/j.jcis.2022.05.005
ER  - 

@article{
author = "Dučić, Tanja and Alves, Carla S. and Vučinić, Željko and Lázaro-Martínez, Juan M. and Petković, Marijana and Soto, Juan and Mutavdžić, Dragosav and Valle Martínez de Yuso, M. and Radotić, Ksenija and Algarra, Manuel",
year = "2022",
abstract = "S and N-doped carbon dots (S-CDs and N-CDs) and their cisplatin (cis-Pt) derivatives. (S-CDs@cis-Pt and N-CDs@cis-Pt) were tested on two ovarian cancer cell lines: A2780 and A2780 cells resistant to cis-Pt (A2780R). Several spectroscopic techniques were employed to check S-CDs@cis-Pt and N-CDs@cis-Pt: solid- and solution-state nuclear magnetic resonance, matrix-assisted laser desorption, ionization time-of-flight mass spectrometry, and X-ray photoelectron spectroscopy. In addition, synchrotron-based Fourier Transformed Infrared spectro-microscopy was used to evaluate the biochemical changes in cells after treatment with cis-Pt, S-CDs, N-CDs, or S-CDs@cis-Pt and N-CDs@cis-Pt, respectively. Computational chemistry was applied to establish the model for the most stable bond between S-CDs and N-CDs and cis-Pt. The results revealed the successful modification of S-CDs and N-CDs with cis-Pt and the formation of a stable composite system that can be used for drug delivery to cancer cells and likewise to overcome acquired cis-Pt resistance. Nanoparticle treatment of A2780 and A2780R cells led to the changes in their structure of lipids, proteins, and nucleic acids depending on the treatment. The results showed the S-CDs@cis-Pt and N-CDs@cis-Pt might be used in the combination with cis-Pt to treat the adenocarcinoma, thus having a potential to be further developed as drug delivery systems.",
journal = "Journal of Colloid and Interface Science",
title = "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach",
volume = "623",
pages = "226-237",
doi = "10.1016/j.jcis.2022.05.005"
}

Dučić, T., Alves, C. S., Vučinić, Ž., Lázaro-Martínez, J. M., Petković, M., Soto, J., Mutavdžić, D., Valle Martínez de Yuso, M., Radotić, K.,& Algarra, M.. (2022). S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science, 623, 226-237.
https://doi.org/10.1016/j.jcis.2022.05.005

Dučić T, Alves CS, Vučinić Ž, Lázaro-Martínez JM, Petković M, Soto J, Mutavdžić D, Valle Martínez de Yuso M, Radotić K, Algarra M. S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach. in Journal of Colloid and Interface Science. 2022;623:226-237.
doi:10.1016/j.jcis.2022.05.005 .

Dučić, Tanja, Alves, Carla S., Vučinić, Željko, Lázaro-Martínez, Juan M., Petković, Marijana, Soto, Juan, Mutavdžić, Dragosav, Valle Martínez de Yuso, M., Radotić, Ksenija, Algarra, Manuel, "S, N-doped carbon dots-based cisplatin delivery system in adenocarcinoma cells: Spectroscopical and computational approach" in Journal of Colloid and Interface Science, 623 (2022):226-237,
https://doi.org/10.1016/j.jcis.2022.05.005 . .

TY  - JOUR
AU  - Matijević, Milica
AU  - Žakula, Jelena
AU  - Korićanac, Lela
AU  - Radoičić, Marija B.
AU  - Liang, Xinyue
AU  - Mi, Lan
AU  - Filipović Tričković, Jelena G.
AU  - Valenta-Šobot, Ana
AU  - Stanković, Maja N.
AU  - Nakarada, Đura
AU  - Mojović, Miloš
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Nešić, Maja D.
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9908
AB  - In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.
T2  - Photochemical and Photobiological Sciences
T1  - Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles
VL  - 20
IS  - 8
SP  - 1087
EP  - 1098
DO  - 10.1007/s43630-021-00082-2
ER  - 

@article{
author = "Matijević, Milica and Žakula, Jelena and Korićanac, Lela and Radoičić, Marija B. and Liang, Xinyue and Mi, Lan and Filipović Tričković, Jelena G. and Valenta-Šobot, Ana and Stanković, Maja N. and Nakarada, Đura and Mojović, Miloš and Petković, Marijana and Stepić, Milutin and Nešić, Maja D.",
year = "2021",
abstract = "In this study, C-doped TiO2 nanoparticles (C-TiO2) were prepared and tested as a photosensitizer for visible-light-driven photodynamic therapy against cervical cancer cells (HeLa). X-ray diffraction and Transmission Electron Microscopy confirmed the anatase form of nanoparticles, spherical shape, and size distribution from 5 to 15 nm. Ultraviolet–visible light spectroscopy showed that C doping of TiO2 enhances the optical absorption in the visible light range caused by a bandgap narrowing. The photo-cytotoxic activity of C-TiO2 was investigated in vitro against HeLa cells. The lack of dark cytotoxicity indicates good biocompatibility of C-TiO2. In contrast, a combination with blue light significantly reduced the survival of HeLa cells: illumination only decreased cell viability by 30% (15 min of illumination, 120 µW power), and 60% when HeLa cells were preincubated with C-TiO2. We have also confirmed blue light-induced C-TiO2-catalyzed generation of reactive oxygen species in vitro and intracellularly. Oxidative stress triggered by C-TiO2/blue light was the leading cause of HeLa cell death. Fluorescent labeling of treated HeLa cells showed distinct morphological changes after the C-TiO2/blue light treatment. Unlike blue light illumination, which caused the appearance of large necrotic cells with deformed nuclei, cytoplasm swelling, and membrane blebbing, a combination of C-TiO2/blue light leads to controlled cell death, thus providing a better outcome of local anticancer therapy.",
journal = "Photochemical and Photobiological Sciences",
title = "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles",
volume = "20",
number = "8",
pages = "1087-1098",
doi = "10.1007/s43630-021-00082-2"
}

Matijević, M., Žakula, J., Korićanac, L., Radoičić, M. B., Liang, X., Mi, L., Filipović Tričković, J. G., Valenta-Šobot, A., Stanković, M. N., Nakarada, Đ., Mojović, M., Petković, M., Stepić, M.,& Nešić, M. D.. (2021). Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences, 20(8), 1087-1098.
https://doi.org/10.1007/s43630-021-00082-2

Matijević M, Žakula J, Korićanac L, Radoičić MB, Liang X, Mi L, Filipović Tričković JG, Valenta-Šobot A, Stanković MN, Nakarada Đ, Mojović M, Petković M, Stepić M, Nešić MD. Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles. in Photochemical and Photobiological Sciences. 2021;20(8):1087-1098.
doi:10.1007/s43630-021-00082-2 .

Matijević, Milica, Žakula, Jelena, Korićanac, Lela, Radoičić, Marija B., Liang, Xinyue, Mi, Lan, Filipović Tričković, Jelena G., Valenta-Šobot, Ana, Stanković, Maja N., Nakarada, Đura, Mojović, Miloš, Petković, Marijana, Stepić, Milutin, Nešić, Maja D., "Controlled killing of human cervical cancer cells by combined action of blue light and C-doped TiO2 nanoparticles" in Photochemical and Photobiological Sciences, 20, no. 8 (2021):1087-1098,
https://doi.org/10.1007/s43630-021-00082-2 . .

TY  - JOUR
AU  - Nešić, Maja D.
AU  - Algarra, Manuel
AU  - Soto, Juan
AU  - Nenadović, Miloš
AU  - Popović, Iva A.
AU  - Petković, Marijana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9930
AB  - Metal acetate complexes are highly interesting in designing and synthesizing metal–organic frameworks, which have potential applications in gas storage, molecular sieves, ion exchange, and catalysis. Cadmium is well recognized as an industrial pollutant and a food contaminant that severely affects human health. For these reasons, the detection and analysis of Cd are of great interest. A major challenge in studying cadmium compounds' structure is that Cd2+ can have a wide range of coordination numbers and diverse geometries. (MA)LDI is extremely useful for detecting Cd(OAc)2·2H2O and investigating Cd's complex and flexible nature. We applied LDI to detect and characterize the Cd-related clusters and MALDI approach to investigate the Cd(OAc)2x2H2O reactivity and detectability with the five different organic matrices. The results revealed that in the solid-state Cd(OAc)2x2H2O tend to form coordination polymers, and in the presence of coordinating ligands (matrix molecules) form smaller complexes. In addition, the correlation between Cd-related LOD and matrix features was observed and discussed, indicating that the LODs of this analyte strongly depend on the optical absorption of the matrix-analyte adduct.
T2  - Polyhedron
T1  - Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts
VL  - 209
SP  - 115463
DO  - 10.1016/j.poly.2021.115463
ER  - 

@article{
author = "Nešić, Maja D. and Algarra, Manuel and Soto, Juan and Nenadović, Miloš and Popović, Iva A. and Petković, Marijana",
year = "2021",
abstract = "Metal acetate complexes are highly interesting in designing and synthesizing metal–organic frameworks, which have potential applications in gas storage, molecular sieves, ion exchange, and catalysis. Cadmium is well recognized as an industrial pollutant and a food contaminant that severely affects human health. For these reasons, the detection and analysis of Cd are of great interest. A major challenge in studying cadmium compounds' structure is that Cd2+ can have a wide range of coordination numbers and diverse geometries. (MA)LDI is extremely useful for detecting Cd(OAc)2·2H2O and investigating Cd's complex and flexible nature. We applied LDI to detect and characterize the Cd-related clusters and MALDI approach to investigate the Cd(OAc)2x2H2O reactivity and detectability with the five different organic matrices. The results revealed that in the solid-state Cd(OAc)2x2H2O tend to form coordination polymers, and in the presence of coordinating ligands (matrix molecules) form smaller complexes. In addition, the correlation between Cd-related LOD and matrix features was observed and discussed, indicating that the LODs of this analyte strongly depend on the optical absorption of the matrix-analyte adduct.",
journal = "Polyhedron",
title = "Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts",
volume = "209",
pages = "115463",
doi = "10.1016/j.poly.2021.115463"
}

Nešić, M. D., Algarra, M., Soto, J., Nenadović, M., Popović, I. A.,& Petković, M.. (2021). Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts. in Polyhedron, 209, 115463.
https://doi.org/10.1016/j.poly.2021.115463

Nešić MD, Algarra M, Soto J, Nenadović M, Popović IA, Petković M. Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts. in Polyhedron. 2021;209:115463.
doi:10.1016/j.poly.2021.115463 .

Nešić, Maja D., Algarra, Manuel, Soto, Juan, Nenadović, Miloš, Popović, Iva A., Petković, Marijana, "Detection of Cadmium-related ions by MALDI TOF mass spectrometry correlates with physicochemical properties of Cadmium/matrix adducts" in Polyhedron, 209 (2021):115463,
https://doi.org/10.1016/j.poly.2021.115463 . .

TY  - CONF
AU  - Nešić, Maja D.
AU  - Matijević, Milica
AU  - Stepić, Milutin
AU  - Petković, Marijana
AU  - Korićanac, Lela
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12467
AB  - Platinum-based complexes represent the mainstay of treatment for various cancer types. However, their usage is often restricted by numerous side effects or intrinsic and acquired resistance. Therefore, significant research efforts have focused on developing therapeutics based on other transition metals, such as ruthenium 1,2. In this study, effects of transition metal complex, cis-dichlorobis (2,2ʹ-bipyridyl-4,4ʹ-dicarboxylic acid)ruthenium(II) (Ru(II) complex) were analyzed on A375 human melanoma and HeLa cell growth, adhesion ability andmigration. Cell viability assay indicated significant antitumor activity of Ru(II) complex on A375 (~60% of control) up to 72 h after treatment, but not on HeLa cells. However, analysis by clonogenic assay showed that growth of both cell lines was decreased 7 days after treatment. Growth inhibition was followed by G1 phase cell cycle arrest (5–10% G1 increase for A375 and 5–8% for HeLa cells compared to control). Moreover, Ru(II) complex increased adhesivity of A375 and HeLa cells by 11 and 16 % respectively and decreased cell migration, as shown by scratch assay. The obtained results indicate that the analyzed Ru(II) complex is a promising metallodrug, as itinduced growth inhibition of A375 and HeLa cells through induction of G1 arrest and decreased metastatic potential of these cells through the increase ofadhesivity and decrease of cell migration.
C3  - Serbian Biochemical Society Tenth Conference : “Biochemical Insights into Molecular Mechanisms”
T1  - Antitumor effect of Ru(II) complex on A375 and HeLa cell growth, migration and adhesion ability
SP  - 76
EP  - 76
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12467
ER  - 

@conference{
author = "Nešić, Maja D. and Matijević, Milica and Stepić, Milutin and Petković, Marijana and Korićanac, Lela",
year = "2021",
abstract = "Platinum-based complexes represent the mainstay of treatment for various cancer types. However, their usage is often restricted by numerous side effects or intrinsic and acquired resistance. Therefore, significant research efforts have focused on developing therapeutics based on other transition metals, such as ruthenium 1,2. In this study, effects of transition metal complex, cis-dichlorobis (2,2ʹ-bipyridyl-4,4ʹ-dicarboxylic acid)ruthenium(II) (Ru(II) complex) were analyzed on A375 human melanoma and HeLa cell growth, adhesion ability andmigration. Cell viability assay indicated significant antitumor activity of Ru(II) complex on A375 (~60% of control) up to 72 h after treatment, but not on HeLa cells. However, analysis by clonogenic assay showed that growth of both cell lines was decreased 7 days after treatment. Growth inhibition was followed by G1 phase cell cycle arrest (5–10% G1 increase for A375 and 5–8% for HeLa cells compared to control). Moreover, Ru(II) complex increased adhesivity of A375 and HeLa cells by 11 and 16 % respectively and decreased cell migration, as shown by scratch assay. The obtained results indicate that the analyzed Ru(II) complex is a promising metallodrug, as itinduced growth inhibition of A375 and HeLa cells through induction of G1 arrest and decreased metastatic potential of these cells through the increase ofadhesivity and decrease of cell migration.",
journal = "Serbian Biochemical Society Tenth Conference : “Biochemical Insights into Molecular Mechanisms”",
title = "Antitumor effect of Ru(II) complex on A375 and HeLa cell growth, migration and adhesion ability",
pages = "76-76",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12467"
}

Nešić, M. D., Matijević, M., Stepić, M., Petković, M.,& Korićanac, L.. (2021). Antitumor effect of Ru(II) complex on A375 and HeLa cell growth, migration and adhesion ability. in Serbian Biochemical Society Tenth Conference : “Biochemical Insights into Molecular Mechanisms”, 76-76.
https://hdl.handle.net/21.15107/rcub_vinar_12467

Nešić MD, Matijević M, Stepić M, Petković M, Korićanac L. Antitumor effect of Ru(II) complex on A375 and HeLa cell growth, migration and adhesion ability. in Serbian Biochemical Society Tenth Conference : “Biochemical Insights into Molecular Mechanisms”. 2021;:76-76.
https://hdl.handle.net/21.15107/rcub_vinar_12467 .

Nešić, Maja D., Matijević, Milica, Stepić, Milutin, Petković, Marijana, Korićanac, Lela, "Antitumor effect of Ru(II) complex on A375 and HeLa cell growth, migration and adhesion ability" in Serbian Biochemical Society Tenth Conference : “Biochemical Insights into Molecular Mechanisms” (2021):76-76,
https://hdl.handle.net/21.15107/rcub_vinar_12467 .

TY  - JOUR
AU  - Nunes, Nadia
AU  - Popović, Iva
AU  - Abreu, Elder
AU  - Maciel, Dina
AU  - Rodrigues, João
AU  - Soto, Juan
AU  - Algarra, Manuel
AU  - Petković, Marijana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13317
AB  - We studied the possibility of detection of [Ru(η5-C5H5)(PPh3)2Cl] (abbreviated by RuCp) complex as a model system for Ru-based metallodrugs in human urine by using matrix-assisted laser desorption/ionization time-of- flight mass spectrometry (MALDI-TOF MS) without previous purification or removal of inorganic salts. Inorganic salts might prevent the detection of RuCp by MALDI-TOF MS, most likely through the increased number and intensity of background/organic matrix signals. This problem might be overcome by the acquisition of matrix- free spectra and the addition of nanoparticles, such as carbon dots, to the urine solution. Our results suggest that RuCp is easily detectable by MALDI-TOF MS in all acquisition conditions, with the CHCA matrix being the best for acquisition in phosphate-containing solutions, whereas in urine, DHB and matrix-free approach demonstrated the highest sensitivity, precision, and reproducibility. The sensitivity of matrix-free MALDI detection of RuCp could be increased by the addition of carbon dots to the urine. Based on theoretical calculations for all matrix/analyte combinations, the model for the interaction of RuCp with carbon dots was established, and higher sensitivity explained.
T2  - Talanta
T1  - Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity
VL  - 222
SP  - 121551
DO  - 10.1016/j.talanta.2020.121551
ER  - 

@article{
author = "Nunes, Nadia and Popović, Iva and Abreu, Elder and Maciel, Dina and Rodrigues, João and Soto, Juan and Algarra, Manuel and Petković, Marijana",
year = "2021",
abstract = "We studied the possibility of detection of [Ru(η5-C5H5)(PPh3)2Cl] (abbreviated by RuCp) complex as a model system for Ru-based metallodrugs in human urine by using matrix-assisted laser desorption/ionization time-of- flight mass spectrometry (MALDI-TOF MS) without previous purification or removal of inorganic salts. Inorganic salts might prevent the detection of RuCp by MALDI-TOF MS, most likely through the increased number and intensity of background/organic matrix signals. This problem might be overcome by the acquisition of matrix- free spectra and the addition of nanoparticles, such as carbon dots, to the urine solution. Our results suggest that RuCp is easily detectable by MALDI-TOF MS in all acquisition conditions, with the CHCA matrix being the best for acquisition in phosphate-containing solutions, whereas in urine, DHB and matrix-free approach demonstrated the highest sensitivity, precision, and reproducibility. The sensitivity of matrix-free MALDI detection of RuCp could be increased by the addition of carbon dots to the urine. Based on theoretical calculations for all matrix/analyte combinations, the model for the interaction of RuCp with carbon dots was established, and higher sensitivity explained.",
journal = "Talanta",
title = "Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity",
volume = "222",
pages = "121551",
doi = "10.1016/j.talanta.2020.121551"
}

Nunes, N., Popović, I., Abreu, E., Maciel, D., Rodrigues, J., Soto, J., Algarra, M.,& Petković, M.. (2021). Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity. in Talanta, 222, 121551.
https://doi.org/10.1016/j.talanta.2020.121551

Nunes N, Popović I, Abreu E, Maciel D, Rodrigues J, Soto J, Algarra M, Petković M. Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity. in Talanta. 2021;222:121551.
doi:10.1016/j.talanta.2020.121551 .

Nunes, Nadia, Popović, Iva, Abreu, Elder, Maciel, Dina, Rodrigues, João, Soto, Juan, Algarra, Manuel, Petković, Marijana, "Detection of Ru potential metallodrug in human urine by MALDI-TOF mass spectrometry: Validation and options to enhance the sensitivity" in Talanta, 222 (2021):121551,
https://doi.org/10.1016/j.talanta.2020.121551 . .

TY  - CONF
AU  - Matijević, Milica
AU  - Petković, Marijana
AU  - Stepić, Milutin
AU  - Nešić, Maja
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12745
AB  - We have studied the cytotoxicity and phototoxicity of nitrogen-doped TiO2 (N-TiO2) nanoparticles (NPs) of the anatase crystal form for a possible human cervical (HeLa) cancer therapy. TiO2 is biocompatible with living structures (a) and its electrons in the valence band can be excited under ultraviolet (UV) light irradiation to produce reactive oxygen species and kill cancer cells. On the other hand, UV light is harmful and its penetration depth is short, which hinders its applications as a photosensitizer. Thus, we have chosen doping of TiO2 with nitrogen, since it was already shown that N-doping shifts the absorbance towards the visible region and enables photoactivation of the TiO2 with visible light, whereas the biocompatibility is preserved. (b) To assess the biocompatibility of N-TiO2 with the cancer cells, we have treated the HeLa cell line with three different concentrations of N-TiO2 NPs suspensions. After 24h of incubation in the dark, we have observed significant cytotoxicity in samples treated with concentration of 100 μg mL-1 of N-TiO2 NPs, whereas no significant cytotoxicity of the concentrations ≤50 μg mL-1 was detected. Hence, we have selected the treatment of 50 μg mL-1 of the N-TiO2 NPs for phototoxicity and cell imaging. Given that the N-TiO2 absorbs in the region of 400-450 nm, we have examined the viability of the HeLa cancer cell line upon the N-TiO2 treatment followed by continuous blue light irradiation (420 nm, 10 μW) for 15 minutes. The survival fraction of the cells treated with N-TiO2 NPs decreased significantly upon blue light irradiation. The intracellular distribution of N-TiO2 NPs was examined by laser scanning confocal microscopy. Most of the cells survived the treatment after 24h, and the agglomerates of N-TiO2 NPs were distributed mainly inside the cancer cells. The results of viability in the dark show that N-TiO2 NPs are not toxic for HeLa cells in the lower concentrations, and the evidence of cellular uptake points towards their biocompatibility. Furthermore, we have shown that it is feasible to induce cells’ phototoxicity upon treatment with N-TiO2 NPs and blue light irradiation.
C3  - 28th Young Research Fellow Meeting
T1  - Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12745
ER  - 

@conference{
author = "Matijević, Milica and Petković, Marijana and Stepić, Milutin and Nešić, Maja",
year = "2021",
abstract = "We have studied the cytotoxicity and phototoxicity of nitrogen-doped TiO2 (N-TiO2) nanoparticles (NPs) of the anatase crystal form for a possible human cervical (HeLa) cancer therapy. TiO2 is biocompatible with living structures (a) and its electrons in the valence band can be excited under ultraviolet (UV) light irradiation to produce reactive oxygen species and kill cancer cells. On the other hand, UV light is harmful and its penetration depth is short, which hinders its applications as a photosensitizer. Thus, we have chosen doping of TiO2 with nitrogen, since it was already shown that N-doping shifts the absorbance towards the visible region and enables photoactivation of the TiO2 with visible light, whereas the biocompatibility is preserved. (b) To assess the biocompatibility of N-TiO2 with the cancer cells, we have treated the HeLa cell line with three different concentrations of N-TiO2 NPs suspensions. After 24h of incubation in the dark, we have observed significant cytotoxicity in samples treated with concentration of 100 μg mL-1 of N-TiO2 NPs, whereas no significant cytotoxicity of the concentrations ≤50 μg mL-1 was detected. Hence, we have selected the treatment of 50 μg mL-1 of the N-TiO2 NPs for phototoxicity and cell imaging. Given that the N-TiO2 absorbs in the region of 400-450 nm, we have examined the viability of the HeLa cancer cell line upon the N-TiO2 treatment followed by continuous blue light irradiation (420 nm, 10 μW) for 15 minutes. The survival fraction of the cells treated with N-TiO2 NPs decreased significantly upon blue light irradiation. The intracellular distribution of N-TiO2 NPs was examined by laser scanning confocal microscopy. Most of the cells survived the treatment after 24h, and the agglomerates of N-TiO2 NPs were distributed mainly inside the cancer cells. The results of viability in the dark show that N-TiO2 NPs are not toxic for HeLa cells in the lower concentrations, and the evidence of cellular uptake points towards their biocompatibility. Furthermore, we have shown that it is feasible to induce cells’ phototoxicity upon treatment with N-TiO2 NPs and blue light irradiation.",
journal = "28th Young Research Fellow Meeting",
title = "Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12745"
}

Matijević, M., Petković, M., Stepić, M.,& Nešić, M.. (2021). Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells. in 28th Young Research Fellow Meeting.
https://hdl.handle.net/21.15107/rcub_vinar_12745

Matijević M, Petković M, Stepić M, Nešić M. Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells. in 28th Young Research Fellow Meeting. 2021;.
https://hdl.handle.net/21.15107/rcub_vinar_12745 .

Matijević, Milica, Petković, Marijana, Stepić, Milutin, Nešić, Maja, "Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells" in 28th Young Research Fellow Meeting (2021),
https://hdl.handle.net/21.15107/rcub_vinar_12745 .

TY  - JOUR
AU  - Jovanović-Stević, Snežana
AU  - Radisavljević, Snežana
AU  - Scheurer, Andreas
AU  - Ćoćić, Dušan
AU  - Šmit, Biljana
AU  - Petković, Marijana
AU  - Živanović, Marko N.
AU  - Virijević, Katarina
AU  - Petrović, Biljana
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13318
AB  - Four new complexes of Pt(II) and Pd(II), [Pd(L1)Cl]Cl 1, [Pd(L2)Cl]Cl 2, [Pt(L1)Cl]Cl 3 and [Pt(L2)Cl]Cl 4 (where L1 = 2,6-bis(5,6-diphenyl-1,2,4-triazin-3-yl)pyridine and L2 = 2,6-bis(5,6-dipropyl-1,2,4-triazin-3-yl)pyridine), were synthesized. Characterization of the complexes was performed using elemental analysis, IR, 1H NMR spectroscopy and MALDI-TOF mass spectrometry. The substitution reactions of 1-4 complexes with L-methionine (L-met), L-cysteine (L-cys) and guanosine-5'-monophosphate (5'-GMP), were studied spectrophotometrically at physiological conditions. Complexes with ligand L1 (1 or 3) were more reactive than those with ligand L2 (2 or 4) by a factor ranging up to 1.57 and 3.71, respectively. The order of reactivity of the nucleophiles was: L-met > L-cys > 5'-GMP. The interactions of complexes with calf thymus-DNA (CT-DNA) and human serum albumin (HSA) were studied by Uv-Vis absorption and fluorescence emission spectroscopy. Competitive binding studies with intercalative agent ethidium bromide (EB) and minor groove binder Hoechst 33258 were performed as well. All studied complexes can interact with DNA through the intercalation and minor groove binding, where the latter was preferred. The binding constants (103 and 104 M-1) for the interaction of complexes with HSA indicate the moderate binding affinity of complexes 1-4 to protein. The trends in the experimental results of binding studies between complexes 3 and 4 with DNA and HSA were compared to those obtained from the molecular docking study. Biological evaluation of cytotoxicity of 1 and 2 on HCT-116 and MDA-MB-231 cell lines showed significant cytotoxic and prooxidative character, while 2 also exerted extraordinary selectivity towards colon cancer in comparison to breast cancer cells. The nucleophilic substitution reactions, DNA/HSA interactions, molecular docking and biological activity of bis(triazinyl)pyridine complexes of Pt(II) and Pd(II) were studied.
T2  - JBIC Journal of Biological Inorganic Chemistry
T1  - Bis(triazinyl)pyridine complexes of Pt(II) and Pd(II): studies of the nucleophilic substitution reactions, DNA/HSA interactions, molecular docking and biological activity
VL  - 26
IS  - 5
SP  - 625
EP  - 637
DO  - 10.1007/s00775-021-01879-3
ER  - 

@article{
author = "Jovanović-Stević, Snežana and Radisavljević, Snežana and Scheurer, Andreas and Ćoćić, Dušan and Šmit, Biljana and Petković, Marijana and Živanović, Marko N. and Virijević, Katarina and Petrović, Biljana",
year = "2021",
abstract = "Four new complexes of Pt(II) and Pd(II), [Pd(L1)Cl]Cl 1, [Pd(L2)Cl]Cl 2, [Pt(L1)Cl]Cl 3 and [Pt(L2)Cl]Cl 4 (where L1 = 2,6-bis(5,6-diphenyl-1,2,4-triazin-3-yl)pyridine and L2 = 2,6-bis(5,6-dipropyl-1,2,4-triazin-3-yl)pyridine), were synthesized. Characterization of the complexes was performed using elemental analysis, IR, 1H NMR spectroscopy and MALDI-TOF mass spectrometry. The substitution reactions of 1-4 complexes with L-methionine (L-met), L-cysteine (L-cys) and guanosine-5'-monophosphate (5'-GMP), were studied spectrophotometrically at physiological conditions. Complexes with ligand L1 (1 or 3) were more reactive than those with ligand L2 (2 or 4) by a factor ranging up to 1.57 and 3.71, respectively. The order of reactivity of the nucleophiles was: L-met > L-cys > 5'-GMP. The interactions of complexes with calf thymus-DNA (CT-DNA) and human serum albumin (HSA) were studied by Uv-Vis absorption and fluorescence emission spectroscopy. Competitive binding studies with intercalative agent ethidium bromide (EB) and minor groove binder Hoechst 33258 were performed as well. All studied complexes can interact with DNA through the intercalation and minor groove binding, where the latter was preferred. The binding constants (103 and 104 M-1) for the interaction of complexes with HSA indicate the moderate binding affinity of complexes 1-4 to protein. The trends in the experimental results of binding studies between complexes 3 and 4 with DNA and HSA were compared to those obtained from the molecular docking study. Biological evaluation of cytotoxicity of 1 and 2 on HCT-116 and MDA-MB-231 cell lines showed significant cytotoxic and prooxidative character, while 2 also exerted extraordinary selectivity towards colon cancer in comparison to breast cancer cells. The nucleophilic substitution reactions, DNA/HSA interactions, molecular docking and biological activity of bis(triazinyl)pyridine complexes of Pt(II) and Pd(II) were studied.",
journal = "JBIC Journal of Biological Inorganic Chemistry",
title = "Bis(triazinyl)pyridine complexes of Pt(II) and Pd(II): studies of the nucleophilic substitution reactions, DNA/HSA interactions, molecular docking and biological activity",
volume = "26",
number = "5",
pages = "625-637",
doi = "10.1007/s00775-021-01879-3"
}

Jovanović-Stević, S., Radisavljević, S., Scheurer, A., Ćoćić, D., Šmit, B., Petković, M., Živanović, M. N., Virijević, K.,& Petrović, B.. (2021). Bis(triazinyl)pyridine complexes of Pt(II) and Pd(II): studies of the nucleophilic substitution reactions, DNA/HSA interactions, molecular docking and biological activity. in JBIC Journal of Biological Inorganic Chemistry, 26(5), 625-637.
https://doi.org/10.1007/s00775-021-01879-3

Jovanović-Stević S, Radisavljević S, Scheurer A, Ćoćić D, Šmit B, Petković M, Živanović MN, Virijević K, Petrović B. Bis(triazinyl)pyridine complexes of Pt(II) and Pd(II): studies of the nucleophilic substitution reactions, DNA/HSA interactions, molecular docking and biological activity. in JBIC Journal of Biological Inorganic Chemistry. 2021;26(5):625-637.
doi:10.1007/s00775-021-01879-3 .

Jovanović-Stević, Snežana, Radisavljević, Snežana, Scheurer, Andreas, Ćoćić, Dušan, Šmit, Biljana, Petković, Marijana, Živanović, Marko N., Virijević, Katarina, Petrović, Biljana, "Bis(triazinyl)pyridine complexes of Pt(II) and Pd(II): studies of the nucleophilic substitution reactions, DNA/HSA interactions, molecular docking and biological activity" in JBIC Journal of Biological Inorganic Chemistry, 26, no. 5 (2021):625-637,
https://doi.org/10.1007/s00775-021-01879-3 . .

TY  - JOUR
AU  - Perestrelo, Rosa
AU  - Petković, Marijana
AU  - Silva, Catarina Luís
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/13326
AB  - Altered lipid metabolism has been associated with the progression of various cancers, and aberrant expression of enzymes involved in the lipid metabolism has been detected in different stages of cancer. Breast cancer (BC) is one of the cancer types known to be associated with alterations in the lipid metabolism and overexpression of enzymes involved in this metabolism. It has been demonstrated that inhibition of the activity of certain enzymes, such as that of phospholipase A2 in BC cell lines sensitizes these cells and decreases the IC50 values for forthcoming therapy with traditional drugs, such as doxorubicin and tamoxifen. Moreover, other phospholipases, such as phospholipase C and D, are involved in intracellular signal transduction, which emphasizes their importance in cancer development. Finally, BC is assumed to be dependent on the diet and the composition of lipids in nutrients. Despite their importance, analytical approaches that can associate the activity of phospholipases with changes in the lipid composition and distribution in cancer tissues are not yet standardized. In this review, an overview of various analytical platforms that are applied on the study of lipids and phospholipase activity in BC tissues will be given, as well as their association with cancer diagnosis and tumor progression. The methods that are applied to tissues obtained from the BC patients will be emphasized and critically evaluated, regarding their applicability in oncology
T2  - Metabolites
T1  - Analytical Platforms for the Determination of Phospholipid Turnover in Breast Cancer Tissue: Role of Phospholipase Activity in Breast Cancer Development
VL  - 11
IS  - 1
SP  - 32
DO  - 10.3390/metabo11010032
ER  - 

@article{
author = "Perestrelo, Rosa and Petković, Marijana and Silva, Catarina Luís",
year = "2021",
abstract = "Altered lipid metabolism has been associated with the progression of various cancers, and aberrant expression of enzymes involved in the lipid metabolism has been detected in different stages of cancer. Breast cancer (BC) is one of the cancer types known to be associated with alterations in the lipid metabolism and overexpression of enzymes involved in this metabolism. It has been demonstrated that inhibition of the activity of certain enzymes, such as that of phospholipase A2 in BC cell lines sensitizes these cells and decreases the IC50 values for forthcoming therapy with traditional drugs, such as doxorubicin and tamoxifen. Moreover, other phospholipases, such as phospholipase C and D, are involved in intracellular signal transduction, which emphasizes their importance in cancer development. Finally, BC is assumed to be dependent on the diet and the composition of lipids in nutrients. Despite their importance, analytical approaches that can associate the activity of phospholipases with changes in the lipid composition and distribution in cancer tissues are not yet standardized. In this review, an overview of various analytical platforms that are applied on the study of lipids and phospholipase activity in BC tissues will be given, as well as their association with cancer diagnosis and tumor progression. The methods that are applied to tissues obtained from the BC patients will be emphasized and critically evaluated, regarding their applicability in oncology",
journal = "Metabolites",
title = "Analytical Platforms for the Determination of Phospholipid Turnover in Breast Cancer Tissue: Role of Phospholipase Activity in Breast Cancer Development",
volume = "11",
number = "1",
pages = "32",
doi = "10.3390/metabo11010032"
}

Perestrelo, R., Petković, M.,& Silva, C. L.. (2021). Analytical Platforms for the Determination of Phospholipid Turnover in Breast Cancer Tissue: Role of Phospholipase Activity in Breast Cancer Development. in Metabolites, 11(1), 32.
https://doi.org/10.3390/metabo11010032

Perestrelo R, Petković M, Silva CL. Analytical Platforms for the Determination of Phospholipid Turnover in Breast Cancer Tissue: Role of Phospholipase Activity in Breast Cancer Development. in Metabolites. 2021;11(1):32.
doi:10.3390/metabo11010032 .

Perestrelo, Rosa, Petković, Marijana, Silva, Catarina Luís, "Analytical Platforms for the Determination of Phospholipid Turnover in Breast Cancer Tissue: Role of Phospholipase Activity in Breast Cancer Development" in Metabolites, 11, no. 1 (2021):32,
https://doi.org/10.3390/metabo11010032 . .

TY  - JOUR
AU  - Houdová, Dominika
AU  - Soto, Juan
AU  - Castro, Rita
AU  - Rodrigues, João
AU  - Soledad Pino-González, Ma
AU  - Petković, Marijana
AU  - Bandosz, Teresa J
AU  - Algarra, Manuel
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9145
AB  - A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.
T2  - Journal of Colloid and Interface Science
T1  - Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry
VL  - 591
SP  - 373
EP  - 383
DO  - 10.1016/j.jcis.2021.02.004
ER  - 

@article{
author = "Houdová, Dominika and Soto, Juan and Castro, Rita and Rodrigues, João and Soledad Pino-González, Ma and Petković, Marijana and Bandosz, Teresa J and Algarra, Manuel",
year = "2021",
abstract = "A binary system composed of carbon dots (CDs) and N-doped CDs (N-CDs) embedded in an organic matrix was used for the analysis of cholesterol by MALDI (matrix-assisted laser desorption and ionization time-of-flight) mass spectrometry, as a model for detection of small, biologically relevant molecules. The results showed that both CDs are sensitive to the cholesterol and can be used either alone or in a binary system with 2,5-dihydroxybenzoic acid (DHB) to enhance the detection process. It was found that both COOH and NH2 groups on CDs surface contributed to the enhancement in the cholesterol detection by MALDI mass spectrometry in the presence of inorganic cations. Nevertheless, in the presence of NaCl, N-CDs led to a better reproducibility of results. It was due to the coexistence of positive and negative charge on N-CDs surface that led to a homogeneous analyte/substrate distribution, which is an important detection parameter. The enhancing effect of carbon dots was linked to a negative Gibbs energy of the complex formation between CDs, Na+, cholesterol and DHB, and it was supported by theoretical calculations. Moreover, upon the addition of CDs/N-CDs, such features as a low ionization potential, vertical excitation, dipole moment and oscillator strength positively affected the cholesterol detection by MALDI in the presence of Na+. © 2021 Elsevier Inc.",
journal = "Journal of Colloid and Interface Science",
title = "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry",
volume = "591",
pages = "373-383",
doi = "10.1016/j.jcis.2021.02.004"
}

Houdová, D., Soto, J., Castro, R., Rodrigues, J., Soledad Pino-González, M., Petković, M., Bandosz, T. J.,& Algarra, M.. (2021). Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science, 591, 373-383.
https://doi.org/10.1016/j.jcis.2021.02.004

Houdová D, Soto J, Castro R, Rodrigues J, Soledad Pino-González M, Petković M, Bandosz TJ, Algarra M. Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry. in Journal of Colloid and Interface Science. 2021;591:373-383.
doi:10.1016/j.jcis.2021.02.004 .

Houdová, Dominika, Soto, Juan, Castro, Rita, Rodrigues, João, Soledad Pino-González, Ma, Petković, Marijana, Bandosz, Teresa J, Algarra, Manuel, "Chemically heterogeneous carbon dots enhanced cholesterol detection by MALDI TOF mass spectrometry" in Journal of Colloid and Interface Science, 591 (2021):373-383,
https://doi.org/10.1016/j.jcis.2021.02.004 . .