Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells
Само за регистроване кориснике
2021
Конференцијски прилог (Објављена верзија)
Метаподаци
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We have studied the cytotoxicity and phototoxicity of nitrogen-doped TiO2 (N-TiO2) nanoparticles (NPs) of the anatase crystal form for a possible human cervical (HeLa) cancer therapy. TiO2 is biocompatible with living structures (a) and its electrons in the valence band can be excited under ultraviolet (UV) light irradiation to produce reactive oxygen species and kill cancer cells. On the other hand, UV light is harmful and its penetration depth is short, which hinders its applications as a photosensitizer. Thus, we have chosen doping of TiO2 with nitrogen, since it was already shown that N-doping shifts the absorbance towards the visible region and enables photoactivation of the TiO2 with visible light, whereas the biocompatibility is preserved. (b) To assess the biocompatibility of N-TiO2 with the cancer cells, we have treated the HeLa cell line with three different concentrations of N-TiO2 NPs suspensions. After 24h of incubation in the dark, we have observed significant cytotoxicit...y in samples treated with concentration of 100 μg mL-1 of N-TiO2 NPs, whereas no significant cytotoxicity of the concentrations ≤50 μg mL-1 was detected. Hence, we have selected the treatment of 50 μg mL-1 of the N-TiO2 NPs for phototoxicity and cell imaging. Given that the N-TiO2 absorbs in the region of 400-450 nm, we have examined the viability of the HeLa cancer cell line upon the N-TiO2 treatment followed by continuous blue light irradiation (420 nm, 10 μW) for 15 minutes. The survival fraction of the cells treated with N-TiO2 NPs decreased significantly upon blue light irradiation. The intracellular distribution of N-TiO2 NPs was examined by laser scanning confocal microscopy. Most of the cells survived the treatment after 24h, and the agglomerates of N-TiO2 NPs were distributed mainly inside the cancer cells. The results of viability in the dark show that N-TiO2 NPs are not toxic for HeLa cells in the lower concentrations, and the evidence of cellular uptake points towards their biocompatibility. Furthermore, we have shown that it is feasible to induce cells’ phototoxicity upon treatment with N-TiO2 NPs and blue light irradiation.
Извор:
28th Young Research Fellow Meeting, 2021Напомена:
- 28th Young Research Fellow Meeting : Web edition : February 11-12, 2021, Faculté de Pharmacie – Université de Paris.
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Институција/група
VinčaTY - CONF AU - Matijević, Milica AU - Petković, Marijana AU - Stepić, Milutin AU - Nešić, Maja PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12745 AB - We have studied the cytotoxicity and phototoxicity of nitrogen-doped TiO2 (N-TiO2) nanoparticles (NPs) of the anatase crystal form for a possible human cervical (HeLa) cancer therapy. TiO2 is biocompatible with living structures (a) and its electrons in the valence band can be excited under ultraviolet (UV) light irradiation to produce reactive oxygen species and kill cancer cells. On the other hand, UV light is harmful and its penetration depth is short, which hinders its applications as a photosensitizer. Thus, we have chosen doping of TiO2 with nitrogen, since it was already shown that N-doping shifts the absorbance towards the visible region and enables photoactivation of the TiO2 with visible light, whereas the biocompatibility is preserved. (b) To assess the biocompatibility of N-TiO2 with the cancer cells, we have treated the HeLa cell line with three different concentrations of N-TiO2 NPs suspensions. After 24h of incubation in the dark, we have observed significant cytotoxicity in samples treated with concentration of 100 μg mL-1 of N-TiO2 NPs, whereas no significant cytotoxicity of the concentrations ≤50 μg mL-1 was detected. Hence, we have selected the treatment of 50 μg mL-1 of the N-TiO2 NPs for phototoxicity and cell imaging. Given that the N-TiO2 absorbs in the region of 400-450 nm, we have examined the viability of the HeLa cancer cell line upon the N-TiO2 treatment followed by continuous blue light irradiation (420 nm, 10 μW) for 15 minutes. The survival fraction of the cells treated with N-TiO2 NPs decreased significantly upon blue light irradiation. The intracellular distribution of N-TiO2 NPs was examined by laser scanning confocal microscopy. Most of the cells survived the treatment after 24h, and the agglomerates of N-TiO2 NPs were distributed mainly inside the cancer cells. The results of viability in the dark show that N-TiO2 NPs are not toxic for HeLa cells in the lower concentrations, and the evidence of cellular uptake points towards their biocompatibility. Furthermore, we have shown that it is feasible to induce cells’ phototoxicity upon treatment with N-TiO2 NPs and blue light irradiation. C3 - 28th Young Research Fellow Meeting T1 - Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells UR - https://hdl.handle.net/21.15107/rcub_vinar_12745 ER -
@conference{ author = "Matijević, Milica and Petković, Marijana and Stepić, Milutin and Nešić, Maja", year = "2021", abstract = "We have studied the cytotoxicity and phototoxicity of nitrogen-doped TiO2 (N-TiO2) nanoparticles (NPs) of the anatase crystal form for a possible human cervical (HeLa) cancer therapy. TiO2 is biocompatible with living structures (a) and its electrons in the valence band can be excited under ultraviolet (UV) light irradiation to produce reactive oxygen species and kill cancer cells. On the other hand, UV light is harmful and its penetration depth is short, which hinders its applications as a photosensitizer. Thus, we have chosen doping of TiO2 with nitrogen, since it was already shown that N-doping shifts the absorbance towards the visible region and enables photoactivation of the TiO2 with visible light, whereas the biocompatibility is preserved. (b) To assess the biocompatibility of N-TiO2 with the cancer cells, we have treated the HeLa cell line with three different concentrations of N-TiO2 NPs suspensions. After 24h of incubation in the dark, we have observed significant cytotoxicity in samples treated with concentration of 100 μg mL-1 of N-TiO2 NPs, whereas no significant cytotoxicity of the concentrations ≤50 μg mL-1 was detected. Hence, we have selected the treatment of 50 μg mL-1 of the N-TiO2 NPs for phototoxicity and cell imaging. Given that the N-TiO2 absorbs in the region of 400-450 nm, we have examined the viability of the HeLa cancer cell line upon the N-TiO2 treatment followed by continuous blue light irradiation (420 nm, 10 μW) for 15 minutes. The survival fraction of the cells treated with N-TiO2 NPs decreased significantly upon blue light irradiation. The intracellular distribution of N-TiO2 NPs was examined by laser scanning confocal microscopy. Most of the cells survived the treatment after 24h, and the agglomerates of N-TiO2 NPs were distributed mainly inside the cancer cells. The results of viability in the dark show that N-TiO2 NPs are not toxic for HeLa cells in the lower concentrations, and the evidence of cellular uptake points towards their biocompatibility. Furthermore, we have shown that it is feasible to induce cells’ phototoxicity upon treatment with N-TiO2 NPs and blue light irradiation.", journal = "28th Young Research Fellow Meeting", title = "Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells", url = "https://hdl.handle.net/21.15107/rcub_vinar_12745" }
Matijević, M., Petković, M., Stepić, M.,& Nešić, M.. (2021). Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells. in 28th Young Research Fellow Meeting. https://hdl.handle.net/21.15107/rcub_vinar_12745
Matijević M, Petković M, Stepić M, Nešić M. Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells. in 28th Young Research Fellow Meeting. 2021;. https://hdl.handle.net/21.15107/rcub_vinar_12745 .
Matijević, Milica, Petković, Marijana, Stepić, Milutin, Nešić, Maja, "Blue light-enhanced cytotoxic activity of nitrogen-doped TiO2 nanoparticles in human cervical cancer cells" in 28th Young Research Fellow Meeting (2021), https://hdl.handle.net/21.15107/rcub_vinar_12745 .