TY  - JOUR
AU  - Milovanović, Jelena
AU  - Todorović-Raković, Nataša
AU  - Vujasinović, Tijana
AU  - Greenman, John
AU  - Mandušić, Vesna
AU  - Radulović, Marko
PY  - 2022
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10392
AB  - Background Granulysin (GNLY) is a cytolytic and proinflammatory molecule which also acts as an immune alarmin. The multifunctional nature of this molecule has made it challenging to define its full potential as a biomarker in breast cancer.AimTo evaluate the prognostic value of intratumoral GNLY in primary breast cancer patients and its association with established clinicopathological parameters.Patients and methodsThe study included 69 node-negative breast cancer patients with known clinicopathological parameters, all of whom had not received any prior hormonal or chemotherapeutic systemic therapy that would interfere with the course of disease. The median follow-up period was 144 months. Steroid hormone receptor status was determined by ligand-binding assay and HER2 status by chromogenic in situ hybridisation (CISH). Intratumoral GNLY mRNA levels were determined by RT-qPCR. Prognostic performance was evaluated by the receiver operating characteristic (ROC), Cox proportional hazards regression and Kaplan-Meier analysis. Classification of patients into GNLYlow and GNLYhigh subgroups was performed by the use of the outcome-oriented cut-off point categorisation approach.ResultsThere was a significant difference between GNLY values of patients without any recurrences and those with local or distant recurrences (Mann-Whitney test, p = 0.05 and p = 0.02, respectively). None of the tested parameters showed prognostic significance for local and distant recurrences when combined. When distant metastases and local recurrences were separated as events, the best prognostic performance was observed for GNLY as compared with any clinicopathological parameter (AUC=0.24 and p = 0.04 for local events; AUC=0.71 and p = 0.03 for distant events). Local recurrence incidence was 0% for the GNLYhigh subgroup and 19% for the GNLYlow subgroup; however distant recurrence incidence was 24% for the GNLYhigh subgroup but only 3% for the GNLYlow subgroup (Kaplan–Meier analysis). A significant positive correlation was found between intratumoral ER and GNLY levels, and a significant negative correlation between tumour grade and GNLY levels.ConclusionHigh levels of granulysin prognosticate low risk of local recurrence but a high risk of distant metastasis in primary, untreated, breast cancer patients.
T2  - Pathology - Research and Practice
T1  - Can granulysin provide prognostic value in primary breast cancer?
VL  - 237
SP  - 154039
DO  - 10.1016/j.prp.2022.154039
ER  - 

@article{
author = "Milovanović, Jelena and Todorović-Raković, Nataša and Vujasinović, Tijana and Greenman, John and Mandušić, Vesna and Radulović, Marko",
year = "2022",
abstract = "Background Granulysin (GNLY) is a cytolytic and proinflammatory molecule which also acts as an immune alarmin. The multifunctional nature of this molecule has made it challenging to define its full potential as a biomarker in breast cancer.AimTo evaluate the prognostic value of intratumoral GNLY in primary breast cancer patients and its association with established clinicopathological parameters.Patients and methodsThe study included 69 node-negative breast cancer patients with known clinicopathological parameters, all of whom had not received any prior hormonal or chemotherapeutic systemic therapy that would interfere with the course of disease. The median follow-up period was 144 months. Steroid hormone receptor status was determined by ligand-binding assay and HER2 status by chromogenic in situ hybridisation (CISH). Intratumoral GNLY mRNA levels were determined by RT-qPCR. Prognostic performance was evaluated by the receiver operating characteristic (ROC), Cox proportional hazards regression and Kaplan-Meier analysis. Classification of patients into GNLYlow and GNLYhigh subgroups was performed by the use of the outcome-oriented cut-off point categorisation approach.ResultsThere was a significant difference between GNLY values of patients without any recurrences and those with local or distant recurrences (Mann-Whitney test, p = 0.05 and p = 0.02, respectively). None of the tested parameters showed prognostic significance for local and distant recurrences when combined. When distant metastases and local recurrences were separated as events, the best prognostic performance was observed for GNLY as compared with any clinicopathological parameter (AUC=0.24 and p = 0.04 for local events; AUC=0.71 and p = 0.03 for distant events). Local recurrence incidence was 0% for the GNLYhigh subgroup and 19% for the GNLYlow subgroup; however distant recurrence incidence was 24% for the GNLYhigh subgroup but only 3% for the GNLYlow subgroup (Kaplan–Meier analysis). A significant positive correlation was found between intratumoral ER and GNLY levels, and a significant negative correlation between tumour grade and GNLY levels.ConclusionHigh levels of granulysin prognosticate low risk of local recurrence but a high risk of distant metastasis in primary, untreated, breast cancer patients.",
journal = "Pathology - Research and Practice",
title = "Can granulysin provide prognostic value in primary breast cancer?",
volume = "237",
pages = "154039",
doi = "10.1016/j.prp.2022.154039"
}

Milovanović, J., Todorović-Raković, N., Vujasinović, T., Greenman, J., Mandušić, V.,& Radulović, M.. (2022). Can granulysin provide prognostic value in primary breast cancer?. in Pathology - Research and Practice, 237, 154039.
https://doi.org/10.1016/j.prp.2022.154039

Milovanović J, Todorović-Raković N, Vujasinović T, Greenman J, Mandušić V, Radulović M. Can granulysin provide prognostic value in primary breast cancer?. in Pathology - Research and Practice. 2022;237:154039.
doi:10.1016/j.prp.2022.154039 .

Milovanović, Jelena, Todorović-Raković, Nataša, Vujasinović, Tijana, Greenman, John, Mandušić, Vesna, Radulović, Marko, "Can granulysin provide prognostic value in primary breast cancer?" in Pathology - Research and Practice, 237 (2022):154039,
https://doi.org/10.1016/j.prp.2022.154039 . .

TY  - JOUR
AU  - Gajić, Vuk A.
AU  - Vujčić, Ivica
AU  - Dražić, Gordana D.
AU  - Milovanović, Jelena
AU  - Mašić, Slobodan
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10377
AB  - The Kolubara river pollutes the coastal land in the river basin and makes it unsuitable for agricultural activities in that area. Also, contaminated land poses a risk to the environment. Different methods can be used for soil decontamination. These methods include biological treatment/bioremediation, chemical oxidation, soil stabilization, physical methods, such as soil leaching, or treatment with high-energy ionizing radiation. Gamma irradiation of soil is a well-known method of inhibiting microbial activity. This paper investigated the influence of different doses and dose rates of gamma irradiation on microorganisms' decontamination of coastal soil, in the Kolubara river basin. The irradiation effects on reducing the total number of microorganisms and removing mold and pathogenic bacteria from soil samples were examined. Gamma radiation affects the soil's organic matter, causing the formation of free reactive radicals, which act as reducing and oxidizing agents, cleaving C-C bonds, and depolymerizing carbohydrates. It was found that a dose of 3 kGy of gamma radiation, neutralizes all pathogenic bacteria, a dose of 5 kGy deactivates mold in soil samples, and a dose of 10 kGy is optimal to kill all microorganisms in the samples and sterilize exposed soil. The research showed that the dose rate does not significantly affect microbiological decontamination of soil using gamma irradiation. The content of heavy metals in soil was determined, and the obtained values were compared with the remediation limit values prescribed by the regulations. It was concluded that the content of heavy metals in the analyzed soil samples is below the limit of remediation values. The only exception is the slightly increased copper content in one sample. The result of this research is the conclusion that the coastal land from the Kolubara basin can be decontaminated by gamma radiation treatment. This advanced soil treatment technology is available in Serbia because there is an industrial plant for gamma radiation treatment within the Vinča Institute.
T2  - Nuclear Technology and Radiation Protection
T1  - Use of high-energy ionizing radiation for microbiological decontamination of coastal soil in the Kolubara river basin, Serbia
VL  - 36
IS  - 3
SP  - 261
EP  - 270
DO  - 10.2298/NTRP2103261G
ER  - 

@article{
author = "Gajić, Vuk A. and Vujčić, Ivica and Dražić, Gordana D. and Milovanović, Jelena and Mašić, Slobodan",
year = "2021",
abstract = "The Kolubara river pollutes the coastal land in the river basin and makes it unsuitable for agricultural activities in that area. Also, contaminated land poses a risk to the environment. Different methods can be used for soil decontamination. These methods include biological treatment/bioremediation, chemical oxidation, soil stabilization, physical methods, such as soil leaching, or treatment with high-energy ionizing radiation. Gamma irradiation of soil is a well-known method of inhibiting microbial activity. This paper investigated the influence of different doses and dose rates of gamma irradiation on microorganisms' decontamination of coastal soil, in the Kolubara river basin. The irradiation effects on reducing the total number of microorganisms and removing mold and pathogenic bacteria from soil samples were examined. Gamma radiation affects the soil's organic matter, causing the formation of free reactive radicals, which act as reducing and oxidizing agents, cleaving C-C bonds, and depolymerizing carbohydrates. It was found that a dose of 3 kGy of gamma radiation, neutralizes all pathogenic bacteria, a dose of 5 kGy deactivates mold in soil samples, and a dose of 10 kGy is optimal to kill all microorganisms in the samples and sterilize exposed soil. The research showed that the dose rate does not significantly affect microbiological decontamination of soil using gamma irradiation. The content of heavy metals in soil was determined, and the obtained values were compared with the remediation limit values prescribed by the regulations. It was concluded that the content of heavy metals in the analyzed soil samples is below the limit of remediation values. The only exception is the slightly increased copper content in one sample. The result of this research is the conclusion that the coastal land from the Kolubara basin can be decontaminated by gamma radiation treatment. This advanced soil treatment technology is available in Serbia because there is an industrial plant for gamma radiation treatment within the Vinča Institute.",
journal = "Nuclear Technology and Radiation Protection",
title = "Use of high-energy ionizing radiation for microbiological decontamination of coastal soil in the Kolubara river basin, Serbia",
volume = "36",
number = "3",
pages = "261-270",
doi = "10.2298/NTRP2103261G"
}

Gajić, V. A., Vujčić, I., Dražić, G. D., Milovanović, J.,& Mašić, S.. (2021). Use of high-energy ionizing radiation for microbiological decontamination of coastal soil in the Kolubara river basin, Serbia. in Nuclear Technology and Radiation Protection, 36(3), 261-270.
https://doi.org/10.2298/NTRP2103261G

Gajić VA, Vujčić I, Dražić GD, Milovanović J, Mašić S. Use of high-energy ionizing radiation for microbiological decontamination of coastal soil in the Kolubara river basin, Serbia. in Nuclear Technology and Radiation Protection. 2021;36(3):261-270.
doi:10.2298/NTRP2103261G .

Gajić, Vuk A., Vujčić, Ivica, Dražić, Gordana D., Milovanović, Jelena, Mašić, Slobodan, "Use of high-energy ionizing radiation for microbiological decontamination of coastal soil in the Kolubara river basin, Serbia" in Nuclear Technology and Radiation Protection, 36, no. 3 (2021):261-270,
https://doi.org/10.2298/NTRP2103261G . .

TY  - JOUR
AU  - Mijajlović, Marina Ž.
AU  - Nikolić, Miloš V.
AU  - Jevtić, Verica V.
AU  - Ratković, Zoran R.
AU  - Milovanović, Jelena
AU  - Arsenijević, Aleksandar
AU  - Stojanović, Bojana
AU  - Novaković, Slađana B.
AU  - Bogdanović, Goran A.
AU  - Trifunović, Srećko R.
AU  - Radić, Gordana P.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1166
AB  - The syntheses of tetradentate ligand, meso-1,2-diphenyl-ethylenediamine-N,N-di-3-propanoic acid (H-2-1,2-dpheddp) and corresponding platinum(IV) and palladium(II) complexes are reported here. The spectroscopically predicted structure of the obtained palladium(II) complex was confirmed by X-ray analysis. Singe crystals suitable for X-ray measurements were obtained by slow crystallization from a DMSO-water mixture. Cytotoxic effects of platinum(IV), palladium(II) complexes and cisplatin on the 4T1 and Bl6F1 cell lines were determined using the MTT colorimetric technique. The complexes showed a dose dependence on cytotoxic effect toward both cell lines. Both complexes were less active than cisplatin, the exception was concentrations above 62.5 mu M of platinum(IV) complex in the B16F1 cell line.
T2  - Macedonian Journal of Chemistry and Chemical Engineering
T1  - Cytotoxicity of Platinum(Iv) and Palladium(Ii) Complexes with Meso-1,2-Diphenyl-Ethylenediamine-N,N -Di-3-Propanoic Acid. Crystal Structure of [Pd(1,2-Dpheddp)] Complex
VL  - 35
IS  - 1
SP  - 79
EP  - 86
DO  - 10.20450/mjcce.2016.729
ER  - 

@article{
author = "Mijajlović, Marina Ž. and Nikolić, Miloš V. and Jevtić, Verica V. and Ratković, Zoran R. and Milovanović, Jelena and Arsenijević, Aleksandar and Stojanović, Bojana and Novaković, Slađana B. and Bogdanović, Goran A. and Trifunović, Srećko R. and Radić, Gordana P.",
year = "2016",
abstract = "The syntheses of tetradentate ligand, meso-1,2-diphenyl-ethylenediamine-N,N-di-3-propanoic acid (H-2-1,2-dpheddp) and corresponding platinum(IV) and palladium(II) complexes are reported here. The spectroscopically predicted structure of the obtained palladium(II) complex was confirmed by X-ray analysis. Singe crystals suitable for X-ray measurements were obtained by slow crystallization from a DMSO-water mixture. Cytotoxic effects of platinum(IV), palladium(II) complexes and cisplatin on the 4T1 and Bl6F1 cell lines were determined using the MTT colorimetric technique. The complexes showed a dose dependence on cytotoxic effect toward both cell lines. Both complexes were less active than cisplatin, the exception was concentrations above 62.5 mu M of platinum(IV) complex in the B16F1 cell line.",
journal = "Macedonian Journal of Chemistry and Chemical Engineering",
title = "Cytotoxicity of Platinum(Iv) and Palladium(Ii) Complexes with Meso-1,2-Diphenyl-Ethylenediamine-N,N -Di-3-Propanoic Acid. Crystal Structure of [Pd(1,2-Dpheddp)] Complex",
volume = "35",
number = "1",
pages = "79-86",
doi = "10.20450/mjcce.2016.729"
}

Mijajlović, M. Ž., Nikolić, M. V., Jevtić, V. V., Ratković, Z. R., Milovanović, J., Arsenijević, A., Stojanović, B., Novaković, S. B., Bogdanović, G. A., Trifunović, S. R.,& Radić, G. P.. (2016). Cytotoxicity of Platinum(Iv) and Palladium(Ii) Complexes with Meso-1,2-Diphenyl-Ethylenediamine-N,N -Di-3-Propanoic Acid. Crystal Structure of [Pd(1,2-Dpheddp)] Complex. in Macedonian Journal of Chemistry and Chemical Engineering, 35(1), 79-86.
https://doi.org/10.20450/mjcce.2016.729

Mijajlović MŽ, Nikolić MV, Jevtić VV, Ratković ZR, Milovanović J, Arsenijević A, Stojanović B, Novaković SB, Bogdanović GA, Trifunović SR, Radić GP. Cytotoxicity of Platinum(Iv) and Palladium(Ii) Complexes with Meso-1,2-Diphenyl-Ethylenediamine-N,N -Di-3-Propanoic Acid. Crystal Structure of [Pd(1,2-Dpheddp)] Complex. in Macedonian Journal of Chemistry and Chemical Engineering. 2016;35(1):79-86.
doi:10.20450/mjcce.2016.729 .

Mijajlović, Marina Ž., Nikolić, Miloš V., Jevtić, Verica V., Ratković, Zoran R., Milovanović, Jelena, Arsenijević, Aleksandar, Stojanović, Bojana, Novaković, Slađana B., Bogdanović, Goran A., Trifunović, Srećko R., Radić, Gordana P., "Cytotoxicity of Platinum(Iv) and Palladium(Ii) Complexes with Meso-1,2-Diphenyl-Ethylenediamine-N,N -Di-3-Propanoic Acid. Crystal Structure of [Pd(1,2-Dpheddp)] Complex" in Macedonian Journal of Chemistry and Chemical Engineering, 35, no. 1 (2016):79-86,
https://doi.org/10.20450/mjcce.2016.729 . .

TY  - JOUR
AU  - Nikolić, Miloš V.
AU  - Mijajlović, Marina Ž.
AU  - Jevtić, Verica V.
AU  - Ratković, Zoran R.
AU  - Novaković, Slađana B.
AU  - Bogdanović, Goran A.
AU  - Milovanović, Jelena
AU  - Arsenijević, Aleksandar
AU  - Stojanović, Bojana
AU  - Trifunović, Srećko R.
AU  - Radić, Gordana P.
PY  - 2016
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1068
AB  - The spectroscopically predicted structure of the obtained copper(II)-complex with S-ethyl derivative of thiosalicylic acid was confirmed by X-ray structural study and compared to previously reported crystal structure of the Cu complex with S-methyl derivative. Single crystals suitable for X-ray measurements were obtained by slow crystallization from a water solution. Cytotoxic effects of S-alkyl (R = benzyl (L1), methyl (12), ethyl (L3), propyl (IA) and butyl (L5)) derivatives of thiosalicylic acid and the corresponding binuclear copper(II)-complexes on murine colon carcinoma cell lines, 026 and CT26.CL25 and human colon carcinoma cell line HCT-116 were reported here. The analysis of cancer cell viability showed that all the tested complexes had low cytotoxic effect on murine colon carcinoma cell lines, but several times higher cytotoxicity on normal human colon carcinoma cells. (C) 2016 Elsevier B.V. All rights reserved.
PB  - Elsevier
T2  - Journal of Molecular Structure
T1  - Cytotoxicity of copper(II)-complexes with some S-alkyl derivatives of thiosalicylic acid. Crystal structure of the binuclear copper(II)-complex with S-ethyl derivative of thiosalicylic acid
VL  - 1116
SP  - 264
EP  - 271
DO  - 10.1016/j.molstruc.2016.03.058
ER  - 

@article{
author = "Nikolić, Miloš V. and Mijajlović, Marina Ž. and Jevtić, Verica V. and Ratković, Zoran R. and Novaković, Slađana B. and Bogdanović, Goran A. and Milovanović, Jelena and Arsenijević, Aleksandar and Stojanović, Bojana and Trifunović, Srećko R. and Radić, Gordana P.",
year = "2016",
abstract = "The spectroscopically predicted structure of the obtained copper(II)-complex with S-ethyl derivative of thiosalicylic acid was confirmed by X-ray structural study and compared to previously reported crystal structure of the Cu complex with S-methyl derivative. Single crystals suitable for X-ray measurements were obtained by slow crystallization from a water solution. Cytotoxic effects of S-alkyl (R = benzyl (L1), methyl (12), ethyl (L3), propyl (IA) and butyl (L5)) derivatives of thiosalicylic acid and the corresponding binuclear copper(II)-complexes on murine colon carcinoma cell lines, 026 and CT26.CL25 and human colon carcinoma cell line HCT-116 were reported here. The analysis of cancer cell viability showed that all the tested complexes had low cytotoxic effect on murine colon carcinoma cell lines, but several times higher cytotoxicity on normal human colon carcinoma cells. (C) 2016 Elsevier B.V. All rights reserved.",
publisher = "Elsevier",
journal = "Journal of Molecular Structure",
title = "Cytotoxicity of copper(II)-complexes with some S-alkyl derivatives of thiosalicylic acid. Crystal structure of the binuclear copper(II)-complex with S-ethyl derivative of thiosalicylic acid",
volume = "1116",
pages = "264-271",
doi = "10.1016/j.molstruc.2016.03.058"
}

Nikolić, M. V., Mijajlović, M. Ž., Jevtić, V. V., Ratković, Z. R., Novaković, S. B., Bogdanović, G. A., Milovanović, J., Arsenijević, A., Stojanović, B., Trifunović, S. R.,& Radić, G. P.. (2016). Cytotoxicity of copper(II)-complexes with some S-alkyl derivatives of thiosalicylic acid. Crystal structure of the binuclear copper(II)-complex with S-ethyl derivative of thiosalicylic acid. in Journal of Molecular Structure
Elsevier., 1116, 264-271.
https://doi.org/10.1016/j.molstruc.2016.03.058

Nikolić MV, Mijajlović MŽ, Jevtić VV, Ratković ZR, Novaković SB, Bogdanović GA, Milovanović J, Arsenijević A, Stojanović B, Trifunović SR, Radić GP. Cytotoxicity of copper(II)-complexes with some S-alkyl derivatives of thiosalicylic acid. Crystal structure of the binuclear copper(II)-complex with S-ethyl derivative of thiosalicylic acid. in Journal of Molecular Structure. 2016;1116:264-271.
doi:10.1016/j.molstruc.2016.03.058 .

Nikolić, Miloš V., Mijajlović, Marina Ž., Jevtić, Verica V., Ratković, Zoran R., Novaković, Slađana B., Bogdanović, Goran A., Milovanović, Jelena, Arsenijević, Aleksandar, Stojanović, Bojana, Trifunović, Srećko R., Radić, Gordana P., "Cytotoxicity of copper(II)-complexes with some S-alkyl derivatives of thiosalicylic acid. Crystal structure of the binuclear copper(II)-complex with S-ethyl derivative of thiosalicylic acid" in Journal of Molecular Structure, 1116 (2016):264-271,
https://doi.org/10.1016/j.molstruc.2016.03.058 . .