TY  - CHAP
AU  - Radojčić, Marija
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Đorđević, Jelena
AU  - Đorđević, Ana
AU  - Demonacos, Constantinos
AU  - Krstić-Demonacos, Marija
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12040
AB  - Chronic stress is recognized as an etiological factor for the onset and exacerbation of
many psychiatric disorders. Among chronic stressors, those of psychosocial and
emotional origin are considered of particular importance for prominent depletion of
physiological and psychological resources. The key mechanisms underlying deleterious
effects of chronic stress are thought to emerge from the compromised stress response at
the level of hypothalamic-pituitary-adrenal (HPA) axis feedback, and limbic brain
structures, such as hippocampus (HIPPO) and prefrontal cortex (PFC).
In this review we summarize and discuss effects of chronic psychosocial isolation
(CPSI) using animal model of male Wistar rats, housed individually for 21 days lacking
physical and visual contact. CPSI, as an important distress factor for normally gregarious
Wistar rats, resulted in diminishment of serum corticosterone and blood glucose, and did
not alter catecholamine levels, which opposes most other chronic stressors that elevate
stress hormones. In the context of possibly aberrant feedback mechanism at the molecular
level, we discuss altered glucocorticoid receptor (GR) distribution and appearance of GR
phosphoisoform excessively phosphorylated on serine 232 (pGR S232), as well as,
altered activities of JNK and CDK kinases that target GR for phosphorylation. The
appearance of pGR S232 in the nucleus and the mitochondria of HIPPO and PFC is
potentially related to a marked transcriptional activation/repression of several GR
regulated nuclear genes (GR itself, CRH, BDNF) and mitochondrial genes (COX1,
COX3). Another important stress and redox state sensitive transcription factor, nuclear factor kappa B (NFțB) is also discussed in terms of the disturbed redox balance
(illustrated by the altered ratio of the activity of an array of antioxidant enzymes) and
altered proapoptotic/proplastic signalling, since it regulates transcription of a wide array
of genes (like Bcl-2, NCAM). Such cellular conditions, provoked by CPSI, are also
shown to affect susceptibility to mitochondrially triggered apoptosis (illustrated by
redistribution of Bcl family members and DNA fragmentation, more prominent in the
PFC) and to simultaneously affect expression of main neural plasticity protein,
polysialylated NCAM (PSA-NCAM). In summary, we present novel causal connection
between the redox imbalance in the CNS, altered signalling via JNK and CDK kinases,
GR phosphorylation/transactivity and NFțB transactivity, as well as their cellular
imbalance, the parameters which all together yield inadequate CNS and systemic stress
response.
T2  - Horizons in Neuroscience Research
T1  - Effects of Chronic Psychosocial Isolation on Limbic Brain Structures of Wistar Rats
VL  - 5
SP  - 97
EP  - 126
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12040
ER  - 

@inbook{
author = "Radojčić, Marija and Adžić, Miroslav and Nićiforović, Ana and Đorđević, Jelena and Đorđević, Ana and Demonacos, Constantinos and Krstić-Demonacos, Marija",
year = "2011",
abstract = "Chronic stress is recognized as an etiological factor for the onset and exacerbation of
many psychiatric disorders. Among chronic stressors, those of psychosocial and
emotional origin are considered of particular importance for prominent depletion of
physiological and psychological resources. The key mechanisms underlying deleterious
effects of chronic stress are thought to emerge from the compromised stress response at
the level of hypothalamic-pituitary-adrenal (HPA) axis feedback, and limbic brain
structures, such as hippocampus (HIPPO) and prefrontal cortex (PFC).
In this review we summarize and discuss effects of chronic psychosocial isolation
(CPSI) using animal model of male Wistar rats, housed individually for 21 days lacking
physical and visual contact. CPSI, as an important distress factor for normally gregarious
Wistar rats, resulted in diminishment of serum corticosterone and blood glucose, and did
not alter catecholamine levels, which opposes most other chronic stressors that elevate
stress hormones. In the context of possibly aberrant feedback mechanism at the molecular
level, we discuss altered glucocorticoid receptor (GR) distribution and appearance of GR
phosphoisoform excessively phosphorylated on serine 232 (pGR S232), as well as,
altered activities of JNK and CDK kinases that target GR for phosphorylation. The
appearance of pGR S232 in the nucleus and the mitochondria of HIPPO and PFC is
potentially related to a marked transcriptional activation/repression of several GR
regulated nuclear genes (GR itself, CRH, BDNF) and mitochondrial genes (COX1,
COX3). Another important stress and redox state sensitive transcription factor, nuclear factor kappa B (NFțB) is also discussed in terms of the disturbed redox balance
(illustrated by the altered ratio of the activity of an array of antioxidant enzymes) and
altered proapoptotic/proplastic signalling, since it regulates transcription of a wide array
of genes (like Bcl-2, NCAM). Such cellular conditions, provoked by CPSI, are also
shown to affect susceptibility to mitochondrially triggered apoptosis (illustrated by
redistribution of Bcl family members and DNA fragmentation, more prominent in the
PFC) and to simultaneously affect expression of main neural plasticity protein,
polysialylated NCAM (PSA-NCAM). In summary, we present novel causal connection
between the redox imbalance in the CNS, altered signalling via JNK and CDK kinases,
GR phosphorylation/transactivity and NFțB transactivity, as well as their cellular
imbalance, the parameters which all together yield inadequate CNS and systemic stress
response.",
journal = "Horizons in Neuroscience Research",
booktitle = "Effects of Chronic Psychosocial Isolation on Limbic Brain Structures of Wistar Rats",
volume = "5",
pages = "97-126",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12040"
}

Radojčić, M., Adžić, M., Nićiforović, A., Đorđević, J., Đorđević, A., Demonacos, C.,& Krstić-Demonacos, M.. (2011). Effects of Chronic Psychosocial Isolation on Limbic Brain Structures of Wistar Rats. in Horizons in Neuroscience Research, 5, 97-126.
https://hdl.handle.net/21.15107/rcub_vinar_12040

Radojčić M, Adžić M, Nićiforović A, Đorđević J, Đorđević A, Demonacos C, Krstić-Demonacos M. Effects of Chronic Psychosocial Isolation on Limbic Brain Structures of Wistar Rats. in Horizons in Neuroscience Research. 2011;5:97-126.
https://hdl.handle.net/21.15107/rcub_vinar_12040 .

Radojčić, Marija, Adžić, Miroslav, Nićiforović, Ana, Đorđević, Jelena, Đorđević, Ana, Demonacos, Constantinos, Krstić-Demonacos, Marija, "Effects of Chronic Psychosocial Isolation on Limbic Brain Structures of Wistar Rats" in Horizons in Neuroscience Research, 5 (2011):97-126,
https://hdl.handle.net/21.15107/rcub_vinar_12040 .

TY  - JOUR
AU  - Đorđević, Jelena D.
AU  - Đorđević, Ana D.
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Radojčić, Marija
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4153
AB  - Clinical reports suggest close interactions between stressors, particularly those of long duration, and liver diseases, such as hepatic inflammation, that is proposed to occur via reactive oxygen species. In the present study we have used 21-day social isolation of male Wistar rats as a model of chronic stress to investigate protein expression/activity of liver antioxidant enzymes: superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR), and protein expression of their upstream regulators: glucocorticoid receptor (GR) and nuclear factor kappa B (NFkB). We have also characterized these parameters in either naive or chronically stressed animals that were challenged by 30-min acute immobilization. We found that chronic isolation caused decrease in serum corticosterone (CORT) and blood glucose (GLU), increase in NFkB signaling, and disproportion between CuZnSOD, peroxidases (CAT, GPx) and GLR, thus promoting H2O2 accumulation and prooxidative state in liver. The overall results suggested that chronic stress exaggerated responsiveness to subsequent stressor at the level of CORT and GLU, and potentiated GLR response, but compromised the restoration of oxido-reductive balance due to irreversible alterations in MnSOD and GPx.
T2  - Physiological Research
T1  - Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats
VL  - 59
IS  - 5
SP  - 729
EP  - 736
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4153
ER  - 

@article{
author = "Đorđević, Jelena D. and Đorđević, Ana D. and Adžić, Miroslav and Nićiforović, Ana and Radojčić, Marija",
year = "2010",
abstract = "Clinical reports suggest close interactions between stressors, particularly those of long duration, and liver diseases, such as hepatic inflammation, that is proposed to occur via reactive oxygen species. In the present study we have used 21-day social isolation of male Wistar rats as a model of chronic stress to investigate protein expression/activity of liver antioxidant enzymes: superoxide dismutases (SODs), catalase (CAT), glutathione peroxidase (GPx) and glutathione reductase (GLR), and protein expression of their upstream regulators: glucocorticoid receptor (GR) and nuclear factor kappa B (NFkB). We have also characterized these parameters in either naive or chronically stressed animals that were challenged by 30-min acute immobilization. We found that chronic isolation caused decrease in serum corticosterone (CORT) and blood glucose (GLU), increase in NFkB signaling, and disproportion between CuZnSOD, peroxidases (CAT, GPx) and GLR, thus promoting H2O2 accumulation and prooxidative state in liver. The overall results suggested that chronic stress exaggerated responsiveness to subsequent stressor at the level of CORT and GLU, and potentiated GLR response, but compromised the restoration of oxido-reductive balance due to irreversible alterations in MnSOD and GPx.",
journal = "Physiological Research",
title = "Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats",
volume = "59",
number = "5",
pages = "729-736",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4153"
}

Đorđević, J. D., Đorđević, A. D., Adžić, M., Nićiforović, A.,& Radojčić, M.. (2010). Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats. in Physiological Research, 59(5), 729-736.
https://hdl.handle.net/21.15107/rcub_vinar_4153

Đorđević JD, Đorđević AD, Adžić M, Nićiforović A, Radojčić M. Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats. in Physiological Research. 2010;59(5):729-736.
https://hdl.handle.net/21.15107/rcub_vinar_4153 .

Đorđević, Jelena D., Đorđević, Ana D., Adžić, Miroslav, Nićiforović, Ana, Radojčić, Marija, "Chronic Stress Differentially Affects Antioxidant Enzymes and Modifies the Acute Stress Response in Liver of Wistar Rats" in Physiological Research, 59, no. 5 (2010):729-736,
https://hdl.handle.net/21.15107/rcub_vinar_4153 .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Ruždijić, Sabera
AU  - Kanazir, Dušan T.
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Paraskevopoulou, Elissavet
AU  - Pantelidou, Constantia
AU  - Radojčić, Marija
AU  - Demonacos, Constantinos
AU  - Krstić-Demonacos, Marija
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3999
AB  - The glucocorticoid receptor (GR) signal transduction and transcriptional regulation are efficiently recapitulated when GR is expressed in Saccharomyces cerevisiae. In this report we demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular mechanisms that control this interdependence between yeast and mammalian cells. To assess the effects of GR phosphorylation the mutated GR derivatives T171A, S224A, S232A, S246A were overexpressed and their transcriptional activity was analysed. These receptor derivatives displayed significant hormone inducible transcription when overexpressed in S. cerevisiae. We have established an inducible methionine expression system, which allows the close regulation of the receptor protein levels to analyse the dependence of GR function on its phosphorylation and protein abundance. Using this system we observed that GR S246A mutation increased its activity across all of the GR concentrations tested. The activity of the S224A and S246A mutants was mostly independent of GR protein levels, whereas the WT, T171A and S232A mediated transcription diminished with declining GR protein levels. Our results suggest that GR phosphorylation at specific residues affects its transcriptional functions in a site selective manner and these effects were directly linked to GR dosage. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.
T2  - Steroids
T1  - Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae
VL  - 75
IS  - 6
SP  - 457
EP  - 465
DO  - 10.1016/j.steroids.2010.03.001
ER  - 

@article{
author = "Popović, Nataša M. and Ruždijić, Sabera and Kanazir, Dušan T. and Nićiforović, Ana and Adžić, Miroslav and Paraskevopoulou, Elissavet and Pantelidou, Constantia and Radojčić, Marija and Demonacos, Constantinos and Krstić-Demonacos, Marija",
year = "2010",
abstract = "The glucocorticoid receptor (GR) signal transduction and transcriptional regulation are efficiently recapitulated when GR is expressed in Saccharomyces cerevisiae. In this report we demonstrate that the in vivo GR phosphorylation pattern, hormone dependency and interdependency of phosphorylation events were similar in yeast and mammalian cells. GR phosphorylation at S246 exhibited inhibitory effect on S224 and S232 phosphorylation, suggesting the conservation of molecular mechanisms that control this interdependence between yeast and mammalian cells. To assess the effects of GR phosphorylation the mutated GR derivatives T171A, S224A, S232A, S246A were overexpressed and their transcriptional activity was analysed. These receptor derivatives displayed significant hormone inducible transcription when overexpressed in S. cerevisiae. We have established an inducible methionine expression system, which allows the close regulation of the receptor protein levels to analyse the dependence of GR function on its phosphorylation and protein abundance. Using this system we observed that GR S246A mutation increased its activity across all of the GR concentrations tested. The activity of the S224A and S246A mutants was mostly independent of GR protein levels, whereas the WT, T171A and S232A mediated transcription diminished with declining GR protein levels. Our results suggest that GR phosphorylation at specific residues affects its transcriptional functions in a site selective manner and these effects were directly linked to GR dosage. Crown Copyright (C) 2010 Published by Elsevier Inc. All rights reserved.",
journal = "Steroids",
title = "Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae",
volume = "75",
number = "6",
pages = "457-465",
doi = "10.1016/j.steroids.2010.03.001"
}

Popović, N. M., Ruždijić, S., Kanazir, D. T., Nićiforović, A., Adžić, M., Paraskevopoulou, E., Pantelidou, C., Radojčić, M., Demonacos, C.,& Krstić-Demonacos, M.. (2010). Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae. in Steroids, 75(6), 457-465.
https://doi.org/10.1016/j.steroids.2010.03.001

Popović NM, Ruždijić S, Kanazir DT, Nićiforović A, Adžić M, Paraskevopoulou E, Pantelidou C, Radojčić M, Demonacos C, Krstić-Demonacos M. Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae. in Steroids. 2010;75(6):457-465.
doi:10.1016/j.steroids.2010.03.001 .

Popović, Nataša M., Ruždijić, Sabera, Kanazir, Dušan T., Nićiforović, Ana, Adžić, Miroslav, Paraskevopoulou, Elissavet, Pantelidou, Constantia, Radojčić, Marija, Demonacos, Constantinos, Krstić-Demonacos, Marija, "Site-specific and dose-dependent effects of glucocorticoid receptor phosphorylation in yeast Saccharomyces cerevisiae" in Steroids, 75, no. 6 (2010):457-465,
https://doi.org/10.1016/j.steroids.2010.03.001 . .

TY  - JOUR
AU  - Đorđević, Jelena D.
AU  - Nićiforović, Ana
AU  - Radojčić, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6816
AB  - Altered activities of antioxidant defence system enzymes and the levels of free radicals scavengers have been found to correlate with various physiological or pathological conditions, including stress. The aim of this study was to determine the effects of chronic 21 day isolation stress on antioxidant enzymes (AOEs) expression and activity in Wistar rat liver tissue. The serum corticosterone (CORT) and glucose (GLU) levels were also measured, as one of the most important indicators of stress. Our data revealed that in chronic stress conditions, when both CORT and GLU were low, the AOEs expression was markedly induced. This increase in MnSOD, CuZnSOD, and catalase exhibited similar trend implying efficient detoxification of O(2) radical anion and H(2)O(2). However, this trend was not followed by the respective enzyme activity. While the total SOD activity was induced by the stress, catalase activity remained unaltered. This discrepancy led us to a conclusion that chronic isolation stress may cause oxidant-antioxidant imbalance in rat liver tissue, favoring H(2)O(2) accumulation.
T2  - Russian Journal of Physical Chemistry A
T1  - Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat
VL  - 83
IS  - 9
SP  - 1617
EP  - 1620
DO  - 10.1134/S0036024409090362
ER  - 

@article{
author = "Đorđević, Jelena D. and Nićiforović, Ana and Radojčić, Marija",
year = "2009",
abstract = "Altered activities of antioxidant defence system enzymes and the levels of free radicals scavengers have been found to correlate with various physiological or pathological conditions, including stress. The aim of this study was to determine the effects of chronic 21 day isolation stress on antioxidant enzymes (AOEs) expression and activity in Wistar rat liver tissue. The serum corticosterone (CORT) and glucose (GLU) levels were also measured, as one of the most important indicators of stress. Our data revealed that in chronic stress conditions, when both CORT and GLU were low, the AOEs expression was markedly induced. This increase in MnSOD, CuZnSOD, and catalase exhibited similar trend implying efficient detoxification of O(2) radical anion and H(2)O(2). However, this trend was not followed by the respective enzyme activity. While the total SOD activity was induced by the stress, catalase activity remained unaltered. This discrepancy led us to a conclusion that chronic isolation stress may cause oxidant-antioxidant imbalance in rat liver tissue, favoring H(2)O(2) accumulation.",
journal = "Russian Journal of Physical Chemistry A",
title = "Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat",
volume = "83",
number = "9",
pages = "1617-1620",
doi = "10.1134/S0036024409090362"
}

Đorđević, J. D., Nićiforović, A.,& Radojčić, M.. (2009). Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat. in Russian Journal of Physical Chemistry A, 83(9), 1617-1620.
https://doi.org/10.1134/S0036024409090362

Đorđević JD, Nićiforović A, Radojčić M. Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat. in Russian Journal of Physical Chemistry A. 2009;83(9):1617-1620.
doi:10.1134/S0036024409090362 .

Đorđević, Jelena D., Nićiforović, Ana, Radojčić, Marija, "Antioxidant Enzymes Expression and Activity in Liver of Stressed Wistar Rat" in Russian Journal of Physical Chemistry A, 83, no. 9 (2009):1617-1620,
https://doi.org/10.1134/S0036024409090362 . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Đorđević, Jelena D.
AU  - Đorđević, Ana D.
AU  - Nićiforović, Ana
AU  - Demonacos, Constantinos
AU  - Radoičić, Marija B.
AU  - Krstić-Demonacos, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3848
AB  - Chronic stress and impaired glucocorticoid receptor (GR) feedback are important factors for the compromised hypothalamic-pituitary-adrenal (HPA) axis activity. We investigated the effects of chronic 2 1 day isolation of Wistar rats on the extrinsic negative feedback part of I-IPA axis: hippocampus (HIPPO) and prefrontal cortex (PFC). In addition to serum corticosterone (CORT), we followed GR subcellular localization, GR phosphorylation at serine 232 and serine 246, expression of GR, regulated genes: GR, CRF and brain-derived neurotropic factor (BDNF), and activity of c-Jun N-terminal kinase (JNK) and Cdk5 kinases that phosphorylate GR. These parameters were also determined in animals subjected to acute 30 min immobilization, which was taken as normal adaptive response to stress. In isolated animals, we found decreased CORT, whereas, in animals exposed to acute immobilization, CORT was markedly increased. Even though the GR was predominantly localized in the nucleus of HIPPO and PFC in acute, but not in chronic stress, the expression of GR, CRF, and BDNF genes was similarly regulated under both acute and chronic stresses. Thus, the transcriptional activity of GR under chronic isolation did not seem to be exclusively dependent on high serum CORT levels nor on the subcellular location of the GR protein. Rather, it resulted front the increased Cdk5 activation and phosphorylation of the nuclear GR at serine 232 and the decreased JNK activity reflected in decreased phosphorylation of the nuclear GR at serine 246. Our study suggests that this nuclear isoform of hippocampal and cortical GR may be related to hypocorticism i.e. HPA axis hypoactivity under chronic isolation stress. journal of Endocrinology (2009) 202, 87-97
T2  - Journal of Endocrinology
T1  - Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain
VL  - 202
IS  - 1
SP  - 87
EP  - 97
DO  - 10.1677/JOE-08-0509
ER  - 

@article{
author = "Adžić, Miroslav and Đorđević, Jelena D. and Đorđević, Ana D. and Nićiforović, Ana and Demonacos, Constantinos and Radoičić, Marija B. and Krstić-Demonacos, Marija",
year = "2009",
abstract = "Chronic stress and impaired glucocorticoid receptor (GR) feedback are important factors for the compromised hypothalamic-pituitary-adrenal (HPA) axis activity. We investigated the effects of chronic 2 1 day isolation of Wistar rats on the extrinsic negative feedback part of I-IPA axis: hippocampus (HIPPO) and prefrontal cortex (PFC). In addition to serum corticosterone (CORT), we followed GR subcellular localization, GR phosphorylation at serine 232 and serine 246, expression of GR, regulated genes: GR, CRF and brain-derived neurotropic factor (BDNF), and activity of c-Jun N-terminal kinase (JNK) and Cdk5 kinases that phosphorylate GR. These parameters were also determined in animals subjected to acute 30 min immobilization, which was taken as normal adaptive response to stress. In isolated animals, we found decreased CORT, whereas, in animals exposed to acute immobilization, CORT was markedly increased. Even though the GR was predominantly localized in the nucleus of HIPPO and PFC in acute, but not in chronic stress, the expression of GR, CRF, and BDNF genes was similarly regulated under both acute and chronic stresses. Thus, the transcriptional activity of GR under chronic isolation did not seem to be exclusively dependent on high serum CORT levels nor on the subcellular location of the GR protein. Rather, it resulted front the increased Cdk5 activation and phosphorylation of the nuclear GR at serine 232 and the decreased JNK activity reflected in decreased phosphorylation of the nuclear GR at serine 246. Our study suggests that this nuclear isoform of hippocampal and cortical GR may be related to hypocorticism i.e. HPA axis hypoactivity under chronic isolation stress. journal of Endocrinology (2009) 202, 87-97",
journal = "Journal of Endocrinology",
title = "Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain",
volume = "202",
number = "1",
pages = "87-97",
doi = "10.1677/JOE-08-0509"
}

Adžić, M., Đorđević, J. D., Đorđević, A. D., Nićiforović, A., Demonacos, C., Radoičić, M. B.,& Krstić-Demonacos, M.. (2009). Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain. in Journal of Endocrinology, 202(1), 87-97.
https://doi.org/10.1677/JOE-08-0509

Adžić M, Đorđević JD, Đorđević AD, Nićiforović A, Demonacos C, Radoičić MB, Krstić-Demonacos M. Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain. in Journal of Endocrinology. 2009;202(1):87-97.
doi:10.1677/JOE-08-0509 .

Adžić, Miroslav, Đorđević, Jelena D., Đorđević, Ana D., Nićiforović, Ana, Demonacos, Constantinos, Radoičić, Marija B., Krstić-Demonacos, Marija, "Acute or chronic stress induce cell compartment-specific phosphorylation of glucocorticoid receptor and alter its transcriptional activity in Wistar rat brain" in Journal of Endocrinology, 202, no. 1 (2009):87-97,
https://doi.org/10.1677/JOE-08-0509 . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Đorđević, Ana D.
AU  - Đorđević, Jelena D.
AU  - Nićiforović, Ana
AU  - Radojčić, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3750
AB  - In the present study, we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex and medulla in mature male Wistar rats subjected to three different stress types: acute, chronic, and combined, i.e., chronic followed by acute stress. These parameters were correlated with adrenal activity as judged from serum levels of corticosterone and catecholamines, respectively, as well as with serum levels of ACTH and glucose. Under all three conditions, we observed bilaterally asymmetric and stress-type-independent hypertrophy of whole adrenals, as well as adrenal cortices and medullas. Under acute and combined stress, adrenal hypertrophy was followed by increase of adrenal hormones in the blood serum. However, under chronic stress, both cortical and medullar activities as judged from low or unaltered levels of the respective hormones and glucose were compromised and disconnected from the input signal of ACTH. Since all of the studied adrenal activities could be restored by subsequent acute stress, it is concluded that chronic isolation can be viewed as partly maladaptive stress with characteristics resembling stress resistance rather than the stress exhaustion stage of the general adaptation syndrome.
T2  - Archives of Biological Sciences
T1  - Effect of Different Types of Stress on Adrenal Gland Parameters and Adrenal Hormones in the Blood Serum of Male Wistar Rats
VL  - 61
IS  - 2
SP  - 187
EP  - 194
DO  - 10.2298/ABS0902187A
ER  - 

@article{
author = "Adžić, Miroslav and Đorđević, Ana D. and Đorđević, Jelena D. and Nićiforović, Ana and Radojčić, Marija",
year = "2009",
abstract = "In the present study, we examined gross changes in the mass of whole adrenal glands and that of the adrenal cortex and medulla in mature male Wistar rats subjected to three different stress types: acute, chronic, and combined, i.e., chronic followed by acute stress. These parameters were correlated with adrenal activity as judged from serum levels of corticosterone and catecholamines, respectively, as well as with serum levels of ACTH and glucose. Under all three conditions, we observed bilaterally asymmetric and stress-type-independent hypertrophy of whole adrenals, as well as adrenal cortices and medullas. Under acute and combined stress, adrenal hypertrophy was followed by increase of adrenal hormones in the blood serum. However, under chronic stress, both cortical and medullar activities as judged from low or unaltered levels of the respective hormones and glucose were compromised and disconnected from the input signal of ACTH. Since all of the studied adrenal activities could be restored by subsequent acute stress, it is concluded that chronic isolation can be viewed as partly maladaptive stress with characteristics resembling stress resistance rather than the stress exhaustion stage of the general adaptation syndrome.",
journal = "Archives of Biological Sciences",
title = "Effect of Different Types of Stress on Adrenal Gland Parameters and Adrenal Hormones in the Blood Serum of Male Wistar Rats",
volume = "61",
number = "2",
pages = "187-194",
doi = "10.2298/ABS0902187A"
}

Adžić, M., Đorđević, A. D., Đorđević, J. D., Nićiforović, A.,& Radojčić, M.. (2009). Effect of Different Types of Stress on Adrenal Gland Parameters and Adrenal Hormones in the Blood Serum of Male Wistar Rats. in Archives of Biological Sciences, 61(2), 187-194.
https://doi.org/10.2298/ABS0902187A

Adžić M, Đorđević AD, Đorđević JD, Nićiforović A, Radojčić M. Effect of Different Types of Stress on Adrenal Gland Parameters and Adrenal Hormones in the Blood Serum of Male Wistar Rats. in Archives of Biological Sciences. 2009;61(2):187-194.
doi:10.2298/ABS0902187A .

Adžić, Miroslav, Đorđević, Ana D., Đorđević, Jelena D., Nićiforović, Ana, Radojčić, Marija, "Effect of Different Types of Stress on Adrenal Gland Parameters and Adrenal Hormones in the Blood Serum of Male Wistar Rats" in Archives of Biological Sciences, 61, no. 2 (2009):187-194,
https://doi.org/10.2298/ABS0902187A . .

TY  - JOUR
AU  - Popović, Nataša M.
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Radojčić, Marija
AU  - Demonacos, Constantinos
AU  - Krstić-Demonacos, Marija
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3667
AB  - The glucocorticoid receptor (GR) protein is a cytosolic ligand-dependent transcription factor with numerous functions regulated by post-translational modifications, including phosphorylation/dephosphorylation. Among the functions most extensively affected by GR phosphorylation are the modulation of its transcriptional activity, alterations in its interaction pattern with cofactors, nuclear translocation and selective gene transactivation. Intensive analysis of the intracellular distribution of GR phosphoisoforms and their interaction with proteins of other cellular signalling networks required the use of [gamma-(32)P]ATP as a phosphate donor, and special laboratory protection measures to avoid external irradiation and contamination. In the present study, simple and easy-to-use non-radioactive protein mobility shift assays (NMS assays) were developed using one- and/or two-dimensional gel electrophoresis based on differences in the pI and molecular mass of GR phosphoisoforms. The GR isoforms were immunodetected with specific monoclonal or polyclonal anti-GR antibodies by Western blot in three diverse systems, namely yeast BJ2168 cells expressing wild-type rat GR, rat hepatoma GRH2 cells grown in culture and brain tissue from Wistar rat experimental animals. The results obtained using the NMS assay were similar to previous results obtained with the [gamma-(32)P] ATP standard assay.
T2  - Journal of the Serbian Chemical Society
T1  - Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays
VL  - 74
IS  - 3
SP  - 237
EP  - 244
DO  - 10.2298/JSC0903237P
ER  - 

@article{
author = "Popović, Nataša M. and Nićiforović, Ana and Adžić, Miroslav and Radojčić, Marija and Demonacos, Constantinos and Krstić-Demonacos, Marija",
year = "2009",
abstract = "The glucocorticoid receptor (GR) protein is a cytosolic ligand-dependent transcription factor with numerous functions regulated by post-translational modifications, including phosphorylation/dephosphorylation. Among the functions most extensively affected by GR phosphorylation are the modulation of its transcriptional activity, alterations in its interaction pattern with cofactors, nuclear translocation and selective gene transactivation. Intensive analysis of the intracellular distribution of GR phosphoisoforms and their interaction with proteins of other cellular signalling networks required the use of [gamma-(32)P]ATP as a phosphate donor, and special laboratory protection measures to avoid external irradiation and contamination. In the present study, simple and easy-to-use non-radioactive protein mobility shift assays (NMS assays) were developed using one- and/or two-dimensional gel electrophoresis based on differences in the pI and molecular mass of GR phosphoisoforms. The GR isoforms were immunodetected with specific monoclonal or polyclonal anti-GR antibodies by Western blot in three diverse systems, namely yeast BJ2168 cells expressing wild-type rat GR, rat hepatoma GRH2 cells grown in culture and brain tissue from Wistar rat experimental animals. The results obtained using the NMS assay were similar to previous results obtained with the [gamma-(32)P] ATP standard assay.",
journal = "Journal of the Serbian Chemical Society",
title = "Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays",
volume = "74",
number = "3",
pages = "237-244",
doi = "10.2298/JSC0903237P"
}

Popović, N. M., Nićiforović, A., Adžić, M., Radojčić, M., Demonacos, C.,& Krstić-Demonacos, M.. (2009). Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays. in Journal of the Serbian Chemical Society, 74(3), 237-244.
https://doi.org/10.2298/JSC0903237P

Popović NM, Nićiforović A, Adžić M, Radojčić M, Demonacos C, Krstić-Demonacos M. Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays. in Journal of the Serbian Chemical Society. 2009;74(3):237-244.
doi:10.2298/JSC0903237P .

Popović, Nataša M., Nićiforović, Ana, Adžić, Miroslav, Radojčić, Marija, Demonacos, Constantinos, Krstić-Demonacos, Marija, "Western blot analysis of glucocorticoid receptor phosphoisoforms by one- and two-dimensional electrophoretic assays" in Journal of the Serbian Chemical Society, 74, no. 3 (2009):237-244,
https://doi.org/10.2298/JSC0903237P . .

TY  - JOUR
AU  - Vučić, Vesna
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Radojčić, Marija
AU  - Ruždijić, Sabera
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3467
AB  - The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.
T2  - Investigational New Drugs
T1  - The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells
VL  - 26
IS  - 4
SP  - 309
EP  - 317
DO  - 10.1007/s10637-007-9101-4
ER  - 

@article{
author = "Vučić, Vesna and Nićiforović, Ana and Adžić, Miroslav and Radojčić, Marija and Ruždijić, Sabera",
year = "2008",
abstract = "The antiproliferative and cytotoxic potential of the nucleotide analog 8-Cl-cAMP was tested in PC-3 and DU145 metastatic human prostate cancer cells. The drug was examined as the only therapeutic agent and in combination with ionizing irradiation (IR). Highly synergistic effects of IR and 8-Cl-cAMP were observed in both cell lines when examined by the MTT viability and BrdU proliferation assays. The combination of IR and 8-Cl-cAMP at clinically relevant doses exerted substantial growth inhibition. The combination of IR and 8-Cl-cAMP caused a significant disturbance in the distribution of cell cycle phases. Cell cycle arrest in the sub-G0/G1 phase predominated in both cell lines. The most striking observation was a significant increase in apoptotic PC-3 and DU145 cells. The DU145 cells were three times more sensitive to the combined treatment than PC-3 cells. The initial resistance to IR-induced apoptosis in these p53-deficient prostate cancer cell lines was overcome through an alternative proapoptotic pathway induced by 8-Cl-cAMP. Considering the low effective doses of treatments, improved tumor eradication rates and minimal undesirable side effects, the combination of IR and 8-Cl-cAMP could be the therapy of choice in treating prostate cancer.",
journal = "Investigational New Drugs",
title = "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells",
volume = "26",
number = "4",
pages = "309-317",
doi = "10.1007/s10637-007-9101-4"
}

Vučić, V., Nićiforović, A., Adžić, M., Radojčić, M.,& Ruždijić, S.. (2008). The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs, 26(4), 309-317.
https://doi.org/10.1007/s10637-007-9101-4

Vučić V, Nićiforović A, Adžić M, Radojčić M, Ruždijić S. The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells. in Investigational New Drugs. 2008;26(4):309-317.
doi:10.1007/s10637-007-9101-4 .

Vučić, Vesna, Nićiforović, Ana, Adžić, Miroslav, Radojčić, Marija, Ruždijić, Sabera, "The combination of gamma ionizing radiation and 8-Cl-cAMP induces synergistic cell growth inhibition and induction of apoptosis in human prostate cancer cells" in Investigational New Drugs, 26, no. 4 (2008):309-317,
https://doi.org/10.1007/s10637-007-9101-4 . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Zarić, Božidarka
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana D.
AU  - Jones, David R.
AU  - Radojčić, Marija
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3490
AB  - To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-kappa B and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4(+) lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P LT 0.001) and after (P LT 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P LT 0.001] and 9 Gy [P LT 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4(+) cells was significantly (P LT 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4(+) cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.
T2  - Redox Report
T1  - Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects
VL  - 13
IS  - 1
SP  - 17
EP  - 22
DO  - 10.1179/135100008X259088
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Zarić, Božidarka and Nešković-Konstantinović, Zora and Spasić, Snežana D. and Jones, David R. and Radojčić, Marija",
year = "2008",
abstract = "To compare the effects of ionising radiation on leukocytes from breast cancer patients and healthy subjects ex vivo, the level of NF-kappa B and the antioxidant enzymes manganese-containing superoxide dismutase (Mn-SOD), copper/zinc-containing superoxide dismutase (CuZn-SOD) and catalase (CAT) in combination with flow cytometric analysis of CD4(+) lymphocytes was performed. The level of Mn-SOD protein was significantly increased in the breast cancer study group both before (P LT 0.001) and after (P LT 0.001) irradiation when compared with healthy subjects. Measurements in parallel indicated that the level of CAT protein was significantly higher in the breast cancer study group after irradiation (2 Gy [P LT 0.001] and 9 Gy [P LT 0.05]) when compared with healthy subjects. Although the initial number of lymphocytes in the blood of breast cancer patients was not different from healthy subjects, the percentage of apoptotic CD4(+) cells was significantly (P LT 0.001) lower both before and after irradiation indicating that cell culture conditions induced radioresistance of CD4(+) cells in the blood of breast cancer patients. The data presented in this current study indicate that brief ex vivo culture of peripheral blood leukocytes potentiates oxidative stress imposed by a breast cancer tumour.",
journal = "Redox Report",
title = "Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects",
volume = "13",
number = "1",
pages = "17-22",
doi = "10.1179/135100008X259088"
}

Adžić, M., Nićiforović, A., Zarić, B., Nešković-Konstantinović, Z., Spasić, S. D., Jones, D. R.,& Radojčić, M.. (2008). Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects. in Redox Report, 13(1), 17-22.
https://doi.org/10.1179/135100008X259088

Adžić M, Nićiforović A, Zarić B, Nešković-Konstantinović Z, Spasić SD, Jones DR, Radojčić M. Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects. in Redox Report. 2008;13(1):17-22.
doi:10.1179/135100008X259088 .

Adžić, Miroslav, Nićiforović, Ana, Zarić, Božidarka, Nešković-Konstantinović, Zora, Spasić, Snežana D., Jones, David R., Radojčić, Marija, "Cell culture conditions potentiate differences in the response to ionising radiation of peripheral blood leukocytes isolated from breast cancer patients and healthy subjects" in Redox Report, 13, no. 1 (2008):17-22,
https://doi.org/10.1179/135100008X259088 . .

TY  - JOUR
AU  - Nićiforović, Ana
AU  - Đorđević, Jelena D.
AU  - Adžić, Miroslav
AU  - Vučić, Vesna
AU  - Mitrasinovic, Petar M.
AU  - Radojčić, Marija
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3404
AB  - Molecular mechanisms for the gamma-ionizing radiation (IR) resistance of human prostate cancer cells, PC-3, are not quite clear. Since the low-LET-IR effects are primarily manifested by the generation of reactive oxygen species (ROS), the IR-induced expressions both of ROS-metabolizing antioxidant enzymes, such as Mn- and CuZn superoxide dismutases (SODs) and catalase (Cat), and of the transcriptional nuclear factor-kappaB (NF-kappa B) were explored. A substantial increase in the concentrations of SODs was observed in the cells irradiated by 10 and 20 Gy relative to those irradiated by 0 and 2 Gy, while the Cat and NF-kappa B expressions were found to be fairly stable. A system biology model was developed to shed more light on how MnSOD affects the biological state of cells depending upon the production of H2O2. By raising the initial presence of MnSOD in the 0.7-10 mu M concentration range, the time-dependent concentrations of H2O2 for various initial levels of MnSOD were contrasted. The radioresistance of PC-3 cells is suggested to be associated with the positive, feed-forward vicious circle established between the H2O2-mediated elevation of MnSOD expression.
T2  - Annals of Biomedical Engineering
T1  - Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells
VL  - 36
IS  - 5
SP  - 831
EP  - 838
DO  - 10.1007/s10439-008-9457-4
ER  - 

@article{
author = "Nićiforović, Ana and Đorđević, Jelena D. and Adžić, Miroslav and Vučić, Vesna and Mitrasinovic, Petar M. and Radojčić, Marija",
year = "2008",
abstract = "Molecular mechanisms for the gamma-ionizing radiation (IR) resistance of human prostate cancer cells, PC-3, are not quite clear. Since the low-LET-IR effects are primarily manifested by the generation of reactive oxygen species (ROS), the IR-induced expressions both of ROS-metabolizing antioxidant enzymes, such as Mn- and CuZn superoxide dismutases (SODs) and catalase (Cat), and of the transcriptional nuclear factor-kappaB (NF-kappa B) were explored. A substantial increase in the concentrations of SODs was observed in the cells irradiated by 10 and 20 Gy relative to those irradiated by 0 and 2 Gy, while the Cat and NF-kappa B expressions were found to be fairly stable. A system biology model was developed to shed more light on how MnSOD affects the biological state of cells depending upon the production of H2O2. By raising the initial presence of MnSOD in the 0.7-10 mu M concentration range, the time-dependent concentrations of H2O2 for various initial levels of MnSOD were contrasted. The radioresistance of PC-3 cells is suggested to be associated with the positive, feed-forward vicious circle established between the H2O2-mediated elevation of MnSOD expression.",
journal = "Annals of Biomedical Engineering",
title = "Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells",
volume = "36",
number = "5",
pages = "831-838",
doi = "10.1007/s10439-008-9457-4"
}

Nićiforović, A., Đorđević, J. D., Adžić, M., Vučić, V., Mitrasinovic, P. M.,& Radojčić, M.. (2008). Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells. in Annals of Biomedical Engineering, 36(5), 831-838.
https://doi.org/10.1007/s10439-008-9457-4

Nićiforović A, Đorđević JD, Adžić M, Vučić V, Mitrasinovic PM, Radojčić M. Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells. in Annals of Biomedical Engineering. 2008;36(5):831-838.
doi:10.1007/s10439-008-9457-4 .

Nićiforović, Ana, Đorđević, Jelena D., Adžić, Miroslav, Vučić, Vesna, Mitrasinovic, Petar M., Radojčić, Marija, "Experimental and systems biology studies of the molecular basis for the radioresistance of prostate carcinoma cells" in Annals of Biomedical Engineering, 36, no. 5 (2008):831-838,
https://doi.org/10.1007/s10439-008-9457-4 . .

TY  - CONF
AU  - Đorđević, Jelena
AU  - Adžić, Miroslav
AU  - Đorđević, A.
AU  - Nićiforović, Ana
AU  - Radojčić, Marija B.
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9378
AB  - The antioxidant enzymes (AOEs) expression was studied in Wistar rat liver under two types of stress: acute (immobilization) and chronic (isolation). The acute stress induced increase in blood corticosterone (CORT) and glucose (GLU), but decreased AOEs expression, and such conditions may result in oxidative stress. In contrast to acute stress, in chronic stress conditions, when both CORT and GLU were low, the AOEs expression was markedly induced. This increase in MnSOD, CuZnSOD, and catalase exhibited similar trend implying efficient detoxification of O2 .- and H2O2.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
T1  - Antioxidant enzymes expression in liver of stressed wistar rat
VL  - 1
SP  - 441
EP  - 443
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9378
ER  - 

@conference{
author = "Đorđević, Jelena and Adžić, Miroslav and Đorđević, A. and Nićiforović, Ana and Radojčić, Marija B.",
year = "2008",
abstract = "The antioxidant enzymes (AOEs) expression was studied in Wistar rat liver under two types of stress: acute (immobilization) and chronic (isolation). The acute stress induced increase in blood corticosterone (CORT) and glucose (GLU), but decreased AOEs expression, and such conditions may result in oxidative stress. In contrast to acute stress, in chronic stress conditions, when both CORT and GLU were low, the AOEs expression was markedly induced. This increase in MnSOD, CuZnSOD, and catalase exhibited similar trend implying efficient detoxification of O2 .- and H2O2.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry",
title = "Antioxidant enzymes expression in liver of stressed wistar rat",
volume = "1",
pages = "441-443",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9378"
}

Đorđević, J., Adžić, M., Đorđević, A., Nićiforović, A.,& Radojčić, M. B.. (2008). Antioxidant enzymes expression in liver of stressed wistar rat. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry
Society of Physical Chemists of Serbia., 1, 441-443.
https://hdl.handle.net/21.15107/rcub_vinar_9378

Đorđević J, Adžić M, Đorđević A, Nićiforović A, Radojčić MB. Antioxidant enzymes expression in liver of stressed wistar rat. in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry. 2008;1:441-443.
https://hdl.handle.net/21.15107/rcub_vinar_9378 .

Đorđević, Jelena, Adžić, Miroslav, Đorđević, A., Nićiforović, Ana, Radojčić, Marija B., "Antioxidant enzymes expression in liver of stressed wistar rat" in Physical chemistry 2008 : 9th international conference on fundamental and applied aspects of physical chemistry, 1 (2008):441-443,
https://hdl.handle.net/21.15107/rcub_vinar_9378 .

TY  - CONF
AU  - Nićiforović, Ana
AU  - Đorđević, Jelena D.
AU  - Adžić, Miroslav
AU  - Vučić, Vesna
AU  - Mitrasinovic, Petar M.
AU  - Radojčić, Marija
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6410
C3  - Abstracts of Papers of the American Chemical Society
T1  - MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells
VL  - 234
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6410
ER  - 

@conference{
author = "Nićiforović, Ana and Đorđević, Jelena D. and Adžić, Miroslav and Vučić, Vesna and Mitrasinovic, Petar M. and Radojčić, Marija",
year = "2007",
journal = "Abstracts of Papers of the American Chemical Society",
title = "MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells",
volume = "234",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6410"
}

Nićiforović, A., Đorđević, J. D., Adžić, M., Vučić, V., Mitrasinovic, P. M.,& Radojčić, M.. (2007). MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells. in Abstracts of Papers of the American Chemical Society, 234.
https://hdl.handle.net/21.15107/rcub_vinar_6410

Nićiforović A, Đorđević JD, Adžić M, Vučić V, Mitrasinovic PM, Radojčić M. MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells. in Abstracts of Papers of the American Chemical Society. 2007;234.
https://hdl.handle.net/21.15107/rcub_vinar_6410 .

Nićiforović, Ana, Đorđević, Jelena D., Adžić, Miroslav, Vučić, Vesna, Mitrasinovic, Petar M., Radojčić, Marija, "MEDI 314-Experimental and systems biology studies of the radioresistance of prostate carcinoma cells" in Abstracts of Papers of the American Chemical Society, 234 (2007),
https://hdl.handle.net/21.15107/rcub_vinar_6410 .

TY  - CONF
AU  - Nićiforović, Ana
AU  - Đorđević, Jelena D.
AU  - Adžić, Miroslav
AU  - Vučić, Vesna
AU  - Mitrasinovic, Petar M.
AU  - Radojčić, Marija
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6409
C3  - Abstracts of Papers of the American Chemical Society
T1  - BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells
VL  - 234
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6409
ER  - 

@conference{
author = "Nićiforović, Ana and Đorđević, Jelena D. and Adžić, Miroslav and Vučić, Vesna and Mitrasinovic, Petar M. and Radojčić, Marija",
year = "2007",
journal = "Abstracts of Papers of the American Chemical Society",
title = "BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells",
volume = "234",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6409"
}

Nićiforović, A., Đorđević, J. D., Adžić, M., Vučić, V., Mitrasinovic, P. M.,& Radojčić, M.. (2007). BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells. in Abstracts of Papers of the American Chemical Society, 234.
https://hdl.handle.net/21.15107/rcub_vinar_6409

Nićiforović A, Đorđević JD, Adžić M, Vučić V, Mitrasinovic PM, Radojčić M. BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells. in Abstracts of Papers of the American Chemical Society. 2007;234.
https://hdl.handle.net/21.15107/rcub_vinar_6409 .

Nićiforović, Ana, Đorđević, Jelena D., Adžić, Miroslav, Vučić, Vesna, Mitrasinovic, Petar M., Radojčić, Marija, "BIOL 165-A kinetic study of the radioresistance of prostate carcinoma cells" in Abstracts of Papers of the American Chemical Society, 234 (2007),
https://hdl.handle.net/21.15107/rcub_vinar_6409 .

TY  - JOUR
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Zarić, Božidarka
AU  - Radojčić, Marija
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6723
AB  - Naturally occurring phytoanthracycline, aloin, was used to radiosensitize HeLaS3 human cervix carcinoma cells. The results indicated that the cytotoxic adjuvant effect of aloin was synergistic with gammaionizing radiation at all drug concentrations and comparable to the cytotoxicity of 5-10 Gy ionizing radiation alone. Radiosensitization of HeLaS3 cells was achieved by 60 mu M aloin, which reduced the IC50 dose of ionizing radiation from 3.4 to 2 Gy. Ionizing radiation and aloin alone or in combination are shown to cause perturbation of the HeLaS3 cell-cycle and increase the percentage of cells in the DNA synthesis (S) phase of the cell cycle. While either of the agents applied alone causes programmed cell death by apoptosis, the simultaneous cell damage by both agents through the altered redox balance compromised cell capacity to conduct this program and led to synergic cytotoxic cell death by necrosis.
T2  - Russian Journal of Physical Chemistry A
T1  - Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells
VL  - 81
IS  - 9
SP  - 1463
EP  - 1466
DO  - 10.1134/S0036024407090221
ER  - 

@article{
author = "Nićiforović, Ana and Adžić, Miroslav and Zarić, Božidarka and Radojčić, Marija",
year = "2007",
abstract = "Naturally occurring phytoanthracycline, aloin, was used to radiosensitize HeLaS3 human cervix carcinoma cells. The results indicated that the cytotoxic adjuvant effect of aloin was synergistic with gammaionizing radiation at all drug concentrations and comparable to the cytotoxicity of 5-10 Gy ionizing radiation alone. Radiosensitization of HeLaS3 cells was achieved by 60 mu M aloin, which reduced the IC50 dose of ionizing radiation from 3.4 to 2 Gy. Ionizing radiation and aloin alone or in combination are shown to cause perturbation of the HeLaS3 cell-cycle and increase the percentage of cells in the DNA synthesis (S) phase of the cell cycle. While either of the agents applied alone causes programmed cell death by apoptosis, the simultaneous cell damage by both agents through the altered redox balance compromised cell capacity to conduct this program and led to synergic cytotoxic cell death by necrosis.",
journal = "Russian Journal of Physical Chemistry A",
title = "Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells",
volume = "81",
number = "9",
pages = "1463-1466",
doi = "10.1134/S0036024407090221"
}

Nićiforović, A., Adžić, M., Zarić, B.,& Radojčić, M.. (2007). Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells. in Russian Journal of Physical Chemistry A, 81(9), 1463-1466.
https://doi.org/10.1134/S0036024407090221

Nićiforović A, Adžić M, Zarić B, Radojčić M. Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells. in Russian Journal of Physical Chemistry A. 2007;81(9):1463-1466.
doi:10.1134/S0036024407090221 .

Nićiforović, Ana, Adžić, Miroslav, Zarić, Božidarka, Radojčić, Marija, "Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells" in Russian Journal of Physical Chemistry A, 81, no. 9 (2007):1463-1466,
https://doi.org/10.1134/S0036024407090221 . .

TY  - JOUR
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Spasić, Snežana D.
AU  - Radojčić, Marija
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3353
AB  - The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future.
T2  - Cancer Biology and Therapy
T1  - Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells
VL  - 6
IS  - 8
SP  - 1200
EP  - 1205
DO  - 10.4161/cbt.6.8.4383
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3353
ER  - 

@article{
author = "Nićiforović, Ana and Adžić, Miroslav and Spasić, Snežana D. and Radojčić, Marija",
year = "2007",
abstract = "The anti proliferative and cytotoxic potential of the natural anthracycline aloin from Aloe vera was tested on human uterine carcinoma HeLaS3 cells. Aloin showed a pronounced antiproliferative effect at physiological concentration (IC50 =97 mu M), caused cell cycle arrest in the S phase and markedly increased HeLaS3 cell apoptosis (to 24%). In the concentration range of 20-100 mu M, its action was accompanied by remarkable changes in the activity of almost all antioxidant enzymes: MnSOD activity was increased many fold, while CuZnSOD and iNOS activities were inhibited. Moreover, inhibition of CuZnSOD was shown to occur by direct aloin interaction with the enzyme. As catalase activity was not changed, it is suggested that such conditions were responsible for antiproliferative and cytotoxic effects owing to accumulation of H2O2. Aloin alone was a more potent proapoptotic agent than a 2 Gy fractional dose of ionizing radiation or a combination of the two. Compared to other currently used therapeutics, aloin, due to its less undesirable side effects and antimetastatic potential, may prove to be the agent of choice on which clinical protocols for the treatment of human cervical carcinoma should rely in future.",
journal = "Cancer Biology and Therapy",
title = "Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells",
volume = "6",
number = "8",
pages = "1200-1205",
doi = "10.4161/cbt.6.8.4383",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3353"
}

Nićiforović, A., Adžić, M., Spasić, S. D.,& Radojčić, M.. (2007). Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells. in Cancer Biology and Therapy, 6(8), 1200-1205.
https://doi.org/10.4161/cbt.6.8.4383
https://hdl.handle.net/21.15107/rcub_vinar_3353

Nićiforović A, Adžić M, Spasić SD, Radojčić M. Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells. in Cancer Biology and Therapy. 2007;6(8):1200-1205.
doi:10.4161/cbt.6.8.4383
https://hdl.handle.net/21.15107/rcub_vinar_3353 .

Nićiforović, Ana, Adžić, Miroslav, Spasić, Snežana D., Radojčić, Marija, "Antitumor effects of a natural anthracycline analog (Aloin) involve altered activity of antioxidant enzymes in HeLaS3 cells" in Cancer Biology and Therapy, 6, no. 8 (2007):1200-1205,
https://doi.org/10.4161/cbt.6.8.4383 .,
https://hdl.handle.net/21.15107/rcub_vinar_3353 .

TY  - JOUR
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Spasić, Snežana D.
AU  - Radojčić, Marija
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3307
AB  - Most experimental models, including cell culture studies, have demonstrated that over-expression of manganese superoxide dismutase (MnSOD) in cells bearing a carcinoma phenotype has anti-proliferative and tumour suppression characteristics. In contrast, when cervical carcinoma biopsies express MnSOD, there is a poor prognosis and resistance to radiation therapy. The results herein indicate that human cervical adenocarcinoma (HeLaS3) cells have increased MnSOD activity (up to 50 % of the total SOD activity) due to low expression of its repressor p53 and a high level of oxidative stress arising from the cell culture conditions. High MnSOD activity may be related to I-IcLaS3 cell radioresistance. illustrated by a high IC50 of 3.4 Gy and by a relatively high level of cell viability after gamma irradiation. In contrast to MnSOD activity, cytosolic CuZnSOD activity decreased after ionising radiation. The catalase (Cat) activity was unchanged. IR also increased the nitric oxide synthase (NOS) activity. Such conditions lead to increased concentrations of the superoxide radical, hydrogen peroxide and NO, which together may be responsible for the decreased expression of NF-B-K and unaltered Cat activity. Therefore, the disturbed redox balance within HeLaS3 cells may be responsible for the cytotoxicity observed at higher irradiation doses. It could be concluded that inhibition of the CuZrISOD activity may be an important target for the selective killing of radioresistant cancer cells.
T2  - Journal of the Serbian Chemical Society
T1  - Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?
VL  - 72
IS  - 10
SP  - 945
EP  - 952
DO  - 10.2298/JSC0710945N
ER  - 

@article{
author = "Nićiforović, Ana and Adžić, Miroslav and Spasić, Snežana D. and Radojčić, Marija",
year = "2007",
abstract = "Most experimental models, including cell culture studies, have demonstrated that over-expression of manganese superoxide dismutase (MnSOD) in cells bearing a carcinoma phenotype has anti-proliferative and tumour suppression characteristics. In contrast, when cervical carcinoma biopsies express MnSOD, there is a poor prognosis and resistance to radiation therapy. The results herein indicate that human cervical adenocarcinoma (HeLaS3) cells have increased MnSOD activity (up to 50 % of the total SOD activity) due to low expression of its repressor p53 and a high level of oxidative stress arising from the cell culture conditions. High MnSOD activity may be related to I-IcLaS3 cell radioresistance. illustrated by a high IC50 of 3.4 Gy and by a relatively high level of cell viability after gamma irradiation. In contrast to MnSOD activity, cytosolic CuZnSOD activity decreased after ionising radiation. The catalase (Cat) activity was unchanged. IR also increased the nitric oxide synthase (NOS) activity. Such conditions lead to increased concentrations of the superoxide radical, hydrogen peroxide and NO, which together may be responsible for the decreased expression of NF-B-K and unaltered Cat activity. Therefore, the disturbed redox balance within HeLaS3 cells may be responsible for the cytotoxicity observed at higher irradiation doses. It could be concluded that inhibition of the CuZrISOD activity may be an important target for the selective killing of radioresistant cancer cells.",
journal = "Journal of the Serbian Chemical Society",
title = "Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?",
volume = "72",
number = "10",
pages = "945-952",
doi = "10.2298/JSC0710945N"
}

Nićiforović, A., Adžić, M., Spasić, S. D.,& Radojčić, M.. (2007). Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?. in Journal of the Serbian Chemical Society, 72(10), 945-952.
https://doi.org/10.2298/JSC0710945N

Nićiforović A, Adžić M, Spasić SD, Radojčić M. Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?. in Journal of the Serbian Chemical Society. 2007;72(10):945-952.
doi:10.2298/JSC0710945N .

Nićiforović, Ana, Adžić, Miroslav, Spasić, Snežana D., Radojčić, Marija, "Do altered activities of superoxide dismutases and the level of NF-κB modulate the effects of gamma radiation in HeLaS3 cells?" in Journal of the Serbian Chemical Society, 72, no. 10 (2007):945-952,
https://doi.org/10.2298/JSC0710945N . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Vučić, Vesna
AU  - Nešković-Konstantinović, Zora
AU  - Spasić, Snežana D.
AU  - Jones, David R.
AU  - Radoičić, Marija B.
AU  - Spasić, Mihajlo
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3008
AB  - There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappa B and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappa B and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.
T2  - Redox Report
T1  - Systemic NF-κB activation in blood cells of breast cancer patients
VL  - 11
IS  - 1
SP  - 38
EP  - 44
DO  - 10.1179/135100006X101002
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Vučić, Vesna and Nešković-Konstantinović, Zora and Spasić, Snežana D. and Jones, David R. and Radoičić, Marija B. and Spasić, Mihajlo",
year = "2006",
abstract = "There is a well-established role for reactive oxygen and nitrogen species, chronic inflammation and immune response in the pathogenesis of breast cancer. Complex interactions between breast cancer cells and surrounding blood vessels are prerequisites for cancer growth and invasion. Reports in the literature concerning the systemic response to, and the effect of, common breast cancer therapy on NF-kappa B and antioxidative defence enzyme expression and activity under clinical conditions are scarce. We determined these parameters in whole blood cell lysate from 16 women with breast cancer before and after combined (cyclophosphamide, doxorubicin, 5-fluorouracil; CAF) therapy and compared the results with 16 healthy women. Significantly higher levels of NF-kappa B and Mn-SOD (both their protein level and their activity) were found in breast cancer patients before and after CAF therapy, in comparison with healthy women. In parallel measurements, no change in the level or activity of catalase (CAT) was detected. According to our findings, it appears that breast cancer creates conditions that increase the level of hydrogen peroxide in the circulating cells and that the applied CAF therapy fails to compensate, therefore creating systemic conditions that favour survival and invasion of breast cancer cells.",
journal = "Redox Report",
title = "Systemic NF-κB activation in blood cells of breast cancer patients",
volume = "11",
number = "1",
pages = "38-44",
doi = "10.1179/135100006X101002"
}

Adžić, M., Nićiforović, A., Vučić, V., Nešković-Konstantinović, Z., Spasić, S. D., Jones, D. R., Radoičić, M. B.,& Spasić, M.. (2006). Systemic NF-κB activation in blood cells of breast cancer patients. in Redox Report, 11(1), 38-44.
https://doi.org/10.1179/135100006X101002

Adžić M, Nićiforović A, Vučić V, Nešković-Konstantinović Z, Spasić SD, Jones DR, Radoičić MB, Spasić M. Systemic NF-κB activation in blood cells of breast cancer patients. in Redox Report. 2006;11(1):38-44.
doi:10.1179/135100006X101002 .

Adžić, Miroslav, Nićiforović, Ana, Vučić, Vesna, Nešković-Konstantinović, Zora, Spasić, Snežana D., Jones, David R., Radoičić, Marija B., Spasić, Mihajlo, "Systemic NF-κB activation in blood cells of breast cancer patients" in Redox Report, 11, no. 1 (2006):38-44,
https://doi.org/10.1179/135100006X101002 . .

TY  - CONF
AU  - Prokopović, J.
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Vučić, Vesna
AU  - Zarić, Božidarka
AU  - Radojčić, Marija B.
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9433
AB  - The molecular mechanism of gamma-ionizing radiation (IR) resistance of human prostate cancer cells PC-3 is not known. Since low-LET-IR effects are primarily achieved through generation of reactive oxygen species (ROS), IR-induced expression of ROSmetabolizing antioxidant enzymes, Mn- and CuZn-superoxide dismutase (Mn- and CuZnSOD) and catalase (CAT), and their upstream regulator transcription factor NFκB was followed. Significant elevation of both SODs was found in cells irradiated with 10- and 20 Gy, while CAT and NFkB expression was unchanged. Since, such conditions lead to accumulation of H2O2, it is concluded that radioresistance of PC-3 cells may emerge from positive feed-forward vicious circle established between H2O2 activation of NFκB and elevated MnSOD activity.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
T1  - Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells
SP  - 422
EP  - 424
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9433
ER  - 

@conference{
author = "Prokopović, J. and Adžić, Miroslav and Nićiforović, Ana and Vučić, Vesna and Zarić, Božidarka and Radojčić, Marija B.",
year = "2006",
abstract = "The molecular mechanism of gamma-ionizing radiation (IR) resistance of human prostate cancer cells PC-3 is not known. Since low-LET-IR effects are primarily achieved through generation of reactive oxygen species (ROS), IR-induced expression of ROSmetabolizing antioxidant enzymes, Mn- and CuZn-superoxide dismutase (Mn- and CuZnSOD) and catalase (CAT), and their upstream regulator transcription factor NFκB was followed. Significant elevation of both SODs was found in cells irradiated with 10- and 20 Gy, while CAT and NFkB expression was unchanged. Since, such conditions lead to accumulation of H2O2, it is concluded that radioresistance of PC-3 cells may emerge from positive feed-forward vicious circle established between H2O2 activation of NFκB and elevated MnSOD activity.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry",
title = "Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells",
pages = "422-424",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9433"
}

Prokopović, J., Adžić, M., Nićiforović, A., Vučić, V., Zarić, B.,& Radojčić, M. B.. (2006). Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 422-424.
https://hdl.handle.net/21.15107/rcub_vinar_9433

Prokopović J, Adžić M, Nićiforović A, Vučić V, Zarić B, Radojčić MB. Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry. 2006;:422-424.
https://hdl.handle.net/21.15107/rcub_vinar_9433 .

Prokopović, J., Adžić, Miroslav, Nićiforović, Ana, Vučić, Vesna, Zarić, Božidarka, Radojčić, Marija B., "Superoxide dismutases in radioresistant PC-3 human prostate carcinoma cells" in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry (2006):422-424,
https://hdl.handle.net/21.15107/rcub_vinar_9433 .

TY  - CONF
AU  - Adžić, Miroslav
AU  - Zarić, Božidarka
AU  - Nićiforović, Ana
AU  - Radojčić, Marija B.
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9431
AB  - Neuroendocrine stress (NES) causes stress-hormones increase and alters balance of intracellular reactive oxygen species (ROS) leading to vulnerability of brain neurons. Prolonged alterations of cell redox milieux may also lead to altered expression of redox sensitive transcription factors, such as NFκB, resposible for brain cell protection. In the presented study NFκB p65 (NFκB) levels were determined in hippocampus and brain cortex of Wistar male rats.exposed to stress of different duration The cytosolic NFκB level was unchanged in acute stress (A, 2h), but significantly down-regulated in either chronic (C,21-day) or combined (C+A) stress. The depletion of NFκB from cell cytosol probably reflected its nuclear translocation and decoupling from the repressive action of glucocorticoid receptor. Through initially protective, NFkB overactivity may lead to accumulation of toxic H2O2 and compromise brain cell capacity to restore homeostasis.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
T1  - Redox-sensitive transcription factor NFkB in brain of stressed wistar rats
SP  - 416
EP  - 418
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9431
ER  - 

@conference{
author = "Adžić, Miroslav and Zarić, Božidarka and Nićiforović, Ana and Radojčić, Marija B.",
year = "2006",
abstract = "Neuroendocrine stress (NES) causes stress-hormones increase and alters balance of intracellular reactive oxygen species (ROS) leading to vulnerability of brain neurons. Prolonged alterations of cell redox milieux may also lead to altered expression of redox sensitive transcription factors, such as NFκB, resposible for brain cell protection. In the presented study NFκB p65 (NFκB) levels were determined in hippocampus and brain cortex of Wistar male rats.exposed to stress of different duration The cytosolic NFκB level was unchanged in acute stress (A, 2h), but significantly down-regulated in either chronic (C,21-day) or combined (C+A) stress. The depletion of NFκB from cell cytosol probably reflected its nuclear translocation and decoupling from the repressive action of glucocorticoid receptor. Through initially protective, NFkB overactivity may lead to accumulation of toxic H2O2 and compromise brain cell capacity to restore homeostasis.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry",
title = "Redox-sensitive transcription factor NFkB in brain of stressed wistar rats",
pages = "416-418",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9431"
}

Adžić, M., Zarić, B., Nićiforović, A.,& Radojčić, M. B.. (2006). Redox-sensitive transcription factor NFkB in brain of stressed wistar rats. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 416-418.
https://hdl.handle.net/21.15107/rcub_vinar_9431

Adžić M, Zarić B, Nićiforović A, Radojčić MB. Redox-sensitive transcription factor NFkB in brain of stressed wistar rats. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry. 2006;:416-418.
https://hdl.handle.net/21.15107/rcub_vinar_9431 .

Adžić, Miroslav, Zarić, Božidarka, Nićiforović, Ana, Radojčić, Marija B., "Redox-sensitive transcription factor NFkB in brain of stressed wistar rats" in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry (2006):416-418,
https://hdl.handle.net/21.15107/rcub_vinar_9431 .

TY  - CONF
AU  - Nićiforović, Ana
AU  - Adžić, Miroslav
AU  - Zarić, Božidarka
AU  - Vučić, Vesna
AU  - Radojčić, Marija B.
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9422
AB  - Naturally occurring phytoanthracycline, aloin, was used to radiosensitize HeLaS3 human cervix carcinoma cells. The results indicated that the cytotoxic adjuvant effect of aloin was synergistic with IR at all drug concentrations and comparable to the cytotoxicity of 5-10Gy IR alone. Radiosensitization of HeLaS3 cells was achieved by 60µM aloin which reduced IC50 dose of IR from 3.4- to 2Gy. The cell damage by both agents compromised cell capacity to conduct programmed cell death by apoptosis, and led to the synergic cytotoxic cell death by necrosis.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
T1  - Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells
VL  - 1
SP  - 344
EP  - 346
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9422
ER  - 

@conference{
author = "Nićiforović, Ana and Adžić, Miroslav and Zarić, Božidarka and Vučić, Vesna and Radojčić, Marija B.",
year = "2006",
abstract = "Naturally occurring phytoanthracycline, aloin, was used to radiosensitize HeLaS3 human cervix carcinoma cells. The results indicated that the cytotoxic adjuvant effect of aloin was synergistic with IR at all drug concentrations and comparable to the cytotoxicity of 5-10Gy IR alone. Radiosensitization of HeLaS3 cells was achieved by 60µM aloin which reduced IC50 dose of IR from 3.4- to 2Gy. The cell damage by both agents compromised cell capacity to conduct programmed cell death by apoptosis, and led to the synergic cytotoxic cell death by necrosis.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry",
title = "Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells",
volume = "1",
pages = "344-346",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9422"
}

Nićiforović, A., Adžić, M., Zarić, B., Vučić, V.,& Radojčić, M. B.. (2006). Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 344-346.
https://hdl.handle.net/21.15107/rcub_vinar_9422

Nićiforović A, Adžić M, Zarić B, Vučić V, Radojčić MB. Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells. in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry. 2006;1:344-346.
https://hdl.handle.net/21.15107/rcub_vinar_9422 .

Nićiforović, Ana, Adžić, Miroslav, Zarić, Božidarka, Vučić, Vesna, Radojčić, Marija B., "Adjuvant antiproliferative and cytotoxic effect of aloin in irradiated HeLaS3 cells" in Physical chemistry 2006: 8th international conference on fundemental and applied aspract of physical chemistry, 1 (2006):344-346,
https://hdl.handle.net/21.15107/rcub_vinar_9422 .

TY  - JOUR
AU  - Filipović, Dragana
AU  - Kasapović, Jelena
AU  - Pejić, Snežana
AU  - Nićiforović, Ana
AU  - Pajović, Snežana B.
AU  - Radoičić, Marija
PY  - 2005
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2941
AB  - Specific composition, protein profiles and total SOD activity were analysed in full milk samples obtained from five farms of the Milk Company IMPAZ The effects of several laboratory treatments on milk proteins SDS-PAGE profiles and the respective SOD activity were also followed. The total SOD activity was detected in all full milk samples, and its values varied between 2 and 3 U mg(-1) protein. The enzyme could be partially purified, up to approximate to 5 U mg(-1) protein, by ethanol extraction. The recovered SOD activity in ethanol extract was proportional to the initial full milk SOD activity. The disruption of casein micelles by Ca2+ removal was followed by a significant decrease in SOD activity to 1.24-0.18 U mg(-1) protein. The loss of enzyme activity was ascribed to the changes in milk milieu induced by dissociation of casein micelles.
T2  - Acta Alimentaria
T1  - Superoxide dismutase activity in various fractions of full bovine milk
VL  - 34
IS  - 3
SP  - 219
EP  - 226
DO  - 10.1556/AAlim.34.2005.3.3
ER  - 

@article{
author = "Filipović, Dragana and Kasapović, Jelena and Pejić, Snežana and Nićiforović, Ana and Pajović, Snežana B. and Radoičić, Marija",
year = "2005",
abstract = "Specific composition, protein profiles and total SOD activity were analysed in full milk samples obtained from five farms of the Milk Company IMPAZ The effects of several laboratory treatments on milk proteins SDS-PAGE profiles and the respective SOD activity were also followed. The total SOD activity was detected in all full milk samples, and its values varied between 2 and 3 U mg(-1) protein. The enzyme could be partially purified, up to approximate to 5 U mg(-1) protein, by ethanol extraction. The recovered SOD activity in ethanol extract was proportional to the initial full milk SOD activity. The disruption of casein micelles by Ca2+ removal was followed by a significant decrease in SOD activity to 1.24-0.18 U mg(-1) protein. The loss of enzyme activity was ascribed to the changes in milk milieu induced by dissociation of casein micelles.",
journal = "Acta Alimentaria",
title = "Superoxide dismutase activity in various fractions of full bovine milk",
volume = "34",
number = "3",
pages = "219-226",
doi = "10.1556/AAlim.34.2005.3.3"
}

Filipović, D., Kasapović, J., Pejić, S., Nićiforović, A., Pajović, S. B.,& Radoičić, M.. (2005). Superoxide dismutase activity in various fractions of full bovine milk. in Acta Alimentaria, 34(3), 219-226.
https://doi.org/10.1556/AAlim.34.2005.3.3

Filipović D, Kasapović J, Pejić S, Nićiforović A, Pajović SB, Radoičić M. Superoxide dismutase activity in various fractions of full bovine milk. in Acta Alimentaria. 2005;34(3):219-226.
doi:10.1556/AAlim.34.2005.3.3 .

Filipović, Dragana, Kasapović, Jelena, Pejić, Snežana, Nićiforović, Ana, Pajović, Snežana B., Radoičić, Marija, "Superoxide dismutase activity in various fractions of full bovine milk" in Acta Alimentaria, 34, no. 3 (2005):219-226,
https://doi.org/10.1556/AAlim.34.2005.3.3 . .

TY  - JOUR
AU  - Nićiforović, Ana
AU  - Milosavljević, B.H.
AU  - Radojčić, Marija
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2856
AB  - Radiolytic behaviour of the major vertebrate muscle proteins: fibril Jar myosin (rnolar mass, M-m = 520,000 g/mol) and filament fortning actin (M-m = 42,050 g/mol) was studied using a SDS-polyacrylamide gel electrophoresis and quantified by high precision laser-densitometry. In order to study the OH radical contribution to the radiation damage, purified chicken myosin and actin (4 muM) were prepared in N2O saturated solution and irradiated with 1-3 kGy at Co-60 gamma source. With respect to changes in the molecular mass, the only observed myosin and actin damage was dose dependent agglomeration of proteins. The corresponding radiation chemical yields of 5 x 10(-8) mol J(-1) and 6.3 x 10(-8) mol J(-1) were obtained for myosin and actin, respectively. This result confirmed that only the radiation-induced agglomeration is initiated with the reaction of the OH radical even in the situation where the OH radical concentration produced exceeds the protein concentration 500 times, thus enabling the multi-radical attack to occur.
T2  - Journal of the Serbian Chemical Society
T1  - Gamma-radiation induced agglomeration of chicken muscle myosin and actin
VL  - 69
IS  - 12
SP  - 999
EP  - 1004
DO  - 10.2298/JSC0412999N
ER  - 

@article{
author = "Nićiforović, Ana and Milosavljević, B.H. and Radojčić, Marija",
year = "2004",
abstract = "Radiolytic behaviour of the major vertebrate muscle proteins: fibril Jar myosin (rnolar mass, M-m = 520,000 g/mol) and filament fortning actin (M-m = 42,050 g/mol) was studied using a SDS-polyacrylamide gel electrophoresis and quantified by high precision laser-densitometry. In order to study the OH radical contribution to the radiation damage, purified chicken myosin and actin (4 muM) were prepared in N2O saturated solution and irradiated with 1-3 kGy at Co-60 gamma source. With respect to changes in the molecular mass, the only observed myosin and actin damage was dose dependent agglomeration of proteins. The corresponding radiation chemical yields of 5 x 10(-8) mol J(-1) and 6.3 x 10(-8) mol J(-1) were obtained for myosin and actin, respectively. This result confirmed that only the radiation-induced agglomeration is initiated with the reaction of the OH radical even in the situation where the OH radical concentration produced exceeds the protein concentration 500 times, thus enabling the multi-radical attack to occur.",
journal = "Journal of the Serbian Chemical Society",
title = "Gamma-radiation induced agglomeration of chicken muscle myosin and actin",
volume = "69",
number = "12",
pages = "999-1004",
doi = "10.2298/JSC0412999N"
}

Nićiforović, A., Milosavljević, B.H.,& Radojčić, M.. (2004). Gamma-radiation induced agglomeration of chicken muscle myosin and actin. in Journal of the Serbian Chemical Society, 69(12), 999-1004.
https://doi.org/10.2298/JSC0412999N

Nićiforović A, Milosavljević B, Radojčić M. Gamma-radiation induced agglomeration of chicken muscle myosin and actin. in Journal of the Serbian Chemical Society. 2004;69(12):999-1004.
doi:10.2298/JSC0412999N .

Nićiforović, Ana, Milosavljević, B.H., Radojčić, Marija, "Gamma-radiation induced agglomeration of chicken muscle myosin and actin" in Journal of the Serbian Chemical Society, 69, no. 12 (2004):999-1004,
https://doi.org/10.2298/JSC0412999N . .

TY  - JOUR
AU  - Vučić, Vesna
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Tišma, Nevena
AU  - Ruždijić, Sabera
AU  - Radojčić, Marija B.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10583
AB  - Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant.
T2  - Jugoslovenska medicinska biohemija
T1  - Cell death in irradiated prostate cancer cells assessed by flow cytometry
VL  - 23
IS  - 4
SP  - 343
EP  - 350
DO  - 10.2298/JMH0404343V
ER  - 

@article{
author = "Vučić, Vesna and Adžić, Miroslav and Nićiforović, Ana and Tišma, Nevena and Ruždijić, Sabera and Radojčić, Marija B.",
year = "2004",
abstract = "Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant.",
journal = "Jugoslovenska medicinska biohemija",
title = "Cell death in irradiated prostate cancer cells assessed by flow cytometry",
volume = "23",
number = "4",
pages = "343-350",
doi = "10.2298/JMH0404343V"
}

Vučić, V., Adžić, M., Nićiforović, A., Tišma, N., Ruždijić, S.,& Radojčić, M. B.. (2004). Cell death in irradiated prostate cancer cells assessed by flow cytometry. in Jugoslovenska medicinska biohemija, 23(4), 343-350.
https://doi.org/10.2298/JMH0404343V

Vučić V, Adžić M, Nićiforović A, Tišma N, Ruždijić S, Radojčić MB. Cell death in irradiated prostate cancer cells assessed by flow cytometry. in Jugoslovenska medicinska biohemija. 2004;23(4):343-350.
doi:10.2298/JMH0404343V .

Vučić, Vesna, Adžić, Miroslav, Nićiforović, Ana, Tišma, Nevena, Ruždijić, Sabera, Radojčić, Marija B., "Cell death in irradiated prostate cancer cells assessed by flow cytometry" in Jugoslovenska medicinska biohemija, 23, no. 4 (2004):343-350,
https://doi.org/10.2298/JMH0404343V . .

TY  - JOUR
AU  - Adžić, Miroslav
AU  - Nićiforović, Ana
AU  - Nešković-Konstantinović, Zora
AU  - Radojčić, Marija B.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/10582
AB  - Purpose: Radiation treatment of breast cancer (BC) often results in post-therapy complications. The undesired sequelae could be avoided by the diagnostic screening of biomarkers for prediction of ionizing radiation (IR)-linked injury of healthy tissues. Patients and methods: The expression of antioxidative defence enzymes CuZn- and Mn-superoxide dismutase (CuZnSOD, MnSOD) and tumor suppressor protein p53 was measured in blood cells of 19 women with BC (age groups 30-45 and 46-60 years) and of the respective controls. The proteins were detected by specific immunostaining and quantified by laser-scanning densitometry. Results: Constitutive expression of CuZnSOD was significantly elevated in the group of BC patients (up to 254 arbitrary units, AU/mL) relative to the control group (105-130 AU/mL). The constitutive expression of MnSOD was elevated (up to 94 AU/mL) in the group of BC patients relative to the controls (53-56 AU/mL). p53 was also constitutively more expressed in BC patients (35-42 AU/mL) than in controls (32-33 AU/mL). Both MnSOD and p53 were inducible by 60Co gamma-ray IR (up to 170 AU/mL and 51 AU/mL, respectively) in the BC patient group. The levels of IR-induced p53 correlated inversely with MnSOD levels. Conclusion: The constitutive expression of all 3 proteins could be a useful biomarker for the presence of BC, but only MnSOD overexpression may be the predictive biomarker for selection of BC patients that would be less susceptible to IR-linked complications. © 2004 Zerbinis Medical Publications.
T2  - Journal of Balkan Union of Oncology.
T1  - Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients
VL  - 9
IS  - 3
SP  - 283
EP  - 287
UR  - https://hdl.handle.net/21.15107/rcub_vinar_10582
ER  - 

@article{
author = "Adžić, Miroslav and Nićiforović, Ana and Nešković-Konstantinović, Zora and Radojčić, Marija B.",
year = "2004",
abstract = "Purpose: Radiation treatment of breast cancer (BC) often results in post-therapy complications. The undesired sequelae could be avoided by the diagnostic screening of biomarkers for prediction of ionizing radiation (IR)-linked injury of healthy tissues. Patients and methods: The expression of antioxidative defence enzymes CuZn- and Mn-superoxide dismutase (CuZnSOD, MnSOD) and tumor suppressor protein p53 was measured in blood cells of 19 women with BC (age groups 30-45 and 46-60 years) and of the respective controls. The proteins were detected by specific immunostaining and quantified by laser-scanning densitometry. Results: Constitutive expression of CuZnSOD was significantly elevated in the group of BC patients (up to 254 arbitrary units, AU/mL) relative to the control group (105-130 AU/mL). The constitutive expression of MnSOD was elevated (up to 94 AU/mL) in the group of BC patients relative to the controls (53-56 AU/mL). p53 was also constitutively more expressed in BC patients (35-42 AU/mL) than in controls (32-33 AU/mL). Both MnSOD and p53 were inducible by 60Co gamma-ray IR (up to 170 AU/mL and 51 AU/mL, respectively) in the BC patient group. The levels of IR-induced p53 correlated inversely with MnSOD levels. Conclusion: The constitutive expression of all 3 proteins could be a useful biomarker for the presence of BC, but only MnSOD overexpression may be the predictive biomarker for selection of BC patients that would be less susceptible to IR-linked complications. © 2004 Zerbinis Medical Publications.",
journal = "Journal of Balkan Union of Oncology.",
title = "Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients",
volume = "9",
number = "3",
pages = "283-287",
url = "https://hdl.handle.net/21.15107/rcub_vinar_10582"
}

Adžić, M., Nićiforović, A., Nešković-Konstantinović, Z.,& Radojčić, M. B.. (2004). Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients. in Journal of Balkan Union of Oncology., 9(3), 283-287.
https://hdl.handle.net/21.15107/rcub_vinar_10582

Adžić M, Nićiforović A, Nešković-Konstantinović Z, Radojčić MB. Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients. in Journal of Balkan Union of Oncology.. 2004;9(3):283-287.
https://hdl.handle.net/21.15107/rcub_vinar_10582 .

Adžić, Miroslav, Nićiforović, Ana, Nešković-Konstantinović, Zora, Radojčić, Marija B., "Superoxide dismutases and p53 protein levels in blood cells of breast cancer patients" in Journal of Balkan Union of Oncology., 9, no. 3 (2004):283-287,
https://hdl.handle.net/21.15107/rcub_vinar_10582 .

TY  - CONF
AU  - Nićiforović, Ana
AU  - Radojčić, Marija B.
PY  - 2004
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/9529
AB  - The single 60Co gamma radiation-induced damage of purified chicken myosin and actin in N2O saturated solution is dose dependent protein cross-linking. The differences in myosin and actin conformation and Mm do not influence the type of radiation-induced damage, but they influence the extent of radiation-induced damage, judged by the lower cross linking of fibrillar myosin compared to actin. The radiolytic behavior of myosin and actin in purified forms is different from their radiolysis in intact muscle, according to the absence of protein fragmentation in the former. The results confirm that industrially sterilized meat may contain significant amount (25- 35%) of structurally altered proteins.
PB  - Society of Physical Chemists of Serbia
C3  - Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
T1  - Gamma-radiation-induced damage of chicken myosin and actin
VL  - 1
SP  - 444
EP  - 446
UR  - https://hdl.handle.net/21.15107/rcub_vinar_9529
ER  - 

@conference{
author = "Nićiforović, Ana and Radojčić, Marija B.",
year = "2004",
abstract = "The single 60Co gamma radiation-induced damage of purified chicken myosin and actin in N2O saturated solution is dose dependent protein cross-linking. The differences in myosin and actin conformation and Mm do not influence the type of radiation-induced damage, but they influence the extent of radiation-induced damage, judged by the lower cross linking of fibrillar myosin compared to actin. The radiolytic behavior of myosin and actin in purified forms is different from their radiolysis in intact muscle, according to the absence of protein fragmentation in the former. The results confirm that industrially sterilized meat may contain significant amount (25- 35%) of structurally altered proteins.",
publisher = "Society of Physical Chemists of Serbia",
journal = "Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry",
title = "Gamma-radiation-induced damage of chicken myosin and actin",
volume = "1",
pages = "444-446",
url = "https://hdl.handle.net/21.15107/rcub_vinar_9529"
}

Nićiforović, A.,& Radojčić, M. B.. (2004). Gamma-radiation-induced damage of chicken myosin and actin. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry
Society of Physical Chemists of Serbia., 1, 444-446.
https://hdl.handle.net/21.15107/rcub_vinar_9529

Nićiforović A, Radojčić MB. Gamma-radiation-induced damage of chicken myosin and actin. in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry. 2004;1:444-446.
https://hdl.handle.net/21.15107/rcub_vinar_9529 .

Nićiforović, Ana, Radojčić, Marija B., "Gamma-radiation-induced damage of chicken myosin and actin" in Physical chemistry 2004: 7th international conference on fundemental and applied aspract of physical chemistry, 1 (2004):444-446,
https://hdl.handle.net/21.15107/rcub_vinar_9529 .