Cell death in irradiated prostate cancer cells assessed by flow cytometry
Само за регистроване кориснике
2004
Аутори
Vučić, VesnaAdžić, Miroslav
Nićiforović, Ana
Tišma, Nevena
Ruždijić, Sabera
Radojčić, Marija B.
Чланак у часопису (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant.
Кључне речи:
human prostate cancer / cell death / gama zračenje / gamma-rays / flow-cytometryИзвор:
Jugoslovenska medicinska biohemija, 2004, 23, 4, 343-350Финансирање / пројекти:
- Ministry for Science, Technology and Development of Serbia [BOI-1953]
Колекције
Институција/група
VinčaTY - JOUR AU - Vučić, Vesna AU - Adžić, Miroslav AU - Nićiforović, Ana AU - Tišma, Nevena AU - Ruždijić, Sabera AU - Radojčić, Marija B. PY - 2004 UR - https://vinar.vin.bg.ac.rs/handle/123456789/10583 AB - Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant. T2 - Jugoslovenska medicinska biohemija T1 - Cell death in irradiated prostate cancer cells assessed by flow cytometry VL - 23 IS - 4 SP - 343 EP - 350 DO - 10.2298/JMH0404343V ER -
@article{ author = "Vučić, Vesna and Adžić, Miroslav and Nićiforović, Ana and Tišma, Nevena and Ruždijić, Sabera and Radojčić, Marija B.", year = "2004", abstract = "Despite the significant advances in cancer chemotherapy, radiotherapy still remains a method of choice for treatment of metastatic human prostate cancer. This study presents quantitative analysis of 60Co gamma-radiation effects on cell growth and cell death of metastatic human prostate cancer PC-3 cell line, performed in time (24-72h) and dose (2-20 Gy) dependent manner. The irradiated PC-3 cells were mostly dying by necrosis at late time intervals (72h), while apoptotic cell death was negligible. The EC50 or 50% of cytotoxicity was not achieved within the radiation doses used (2-20 Gy), but significant cell growth inhibition with IC50 of 10.4 Gy was observed. It is concluded that the increase in the radiation dose may have an important cytostatic effect, but for the complete eradication of metastatic prostate cancer novel cytotoxic drugs and radiosensitizers should be introduced as adjuvant.", journal = "Jugoslovenska medicinska biohemija", title = "Cell death in irradiated prostate cancer cells assessed by flow cytometry", volume = "23", number = "4", pages = "343-350", doi = "10.2298/JMH0404343V" }
Vučić, V., Adžić, M., Nićiforović, A., Tišma, N., Ruždijić, S.,& Radojčić, M. B.. (2004). Cell death in irradiated prostate cancer cells assessed by flow cytometry. in Jugoslovenska medicinska biohemija, 23(4), 343-350. https://doi.org/10.2298/JMH0404343V
Vučić V, Adžić M, Nićiforović A, Tišma N, Ruždijić S, Radojčić MB. Cell death in irradiated prostate cancer cells assessed by flow cytometry. in Jugoslovenska medicinska biohemija. 2004;23(4):343-350. doi:10.2298/JMH0404343V .
Vučić, Vesna, Adžić, Miroslav, Nićiforović, Ana, Tišma, Nevena, Ruždijić, Sabera, Radojčić, Marija B., "Cell death in irradiated prostate cancer cells assessed by flow cytometry" in Jugoslovenska medicinska biohemija, 23, no. 4 (2004):343-350, https://doi.org/10.2298/JMH0404343V . .