TY  - JOUR
AU  - Vicic, Drazen D.
AU  - Stoiljković, Milovan
AU  - Bojat, Nenad C.
AU  - Sabovljevic, Marko S.
AU  - Stevanović, Branka M.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/221
AB  - Serpentine (ultramafic) soils are extremely stressful environments for plant growth due to macronutrient deficiency (N, P, K, Ca), macronutrient toxicity (Mg; extremely high Mg:Ca ratio), and micronutrient toxicity (Mn, Fe, Ni, Cu, Zn), as well as toxicity of other heavy metals (Al, Cr, Co). Serpentine soils are also often shallow, rocky, and susceptible to drought. As a result of extreme adverse physical and chemical conditions, serpentine soils support a high proportion of endemic plant species that are adapted to their harsh environment. We analysed root, stem, and leaf tissue element concentrations (Ca, Mg, Al, Mn, Fe, Ni, Cu, and Zn) of Halacsya sendtneri (Boraginaceae; strict serpentine endemic), Cheilanthes marantae (Pteridaceac; broad serpentine endemic/strong indicator), and Seseli rigidum (Apiaccac; weak serpentine indicator/indifferent) growing on serpentine and limestone in Serbia. Element bioaccumulation factor was calculated as the ratio of plant tissue clement concentration to soil plant-available element concentration. Tissue concentrations of Ca and Mg for H. sendtneri and S. rigidum indicate that the species selectively uptake and translocatc Ca to leaves, relative to Mg, to maintain adequate tissue Mg:Ca ratio. C. marantae did not exhibit selective Ca uptake or translocation, but did exhibit Mg sequestration in roots. Heavy metal exclusion and sequestration were the primary physiological tolerance mechanisms conveying serpentine tolerance in the three species. S. rigidum exhibited divergence into serpentine tolerant and limestone tolerant ecotypes, presenting a useful model species for further studies of physiological adaptation to chemically extreme soils.
T2  - Revue D Ecologie. La Terre et la Vie
T1  - Physiological Tolerance Mechanisms of Serpentine Tolerant Plants from Serbia
VL  - 69
IS  - 3-4
SP  - 185
EP  - 195
UR  - https://hdl.handle.net/21.15107/rcub_vinar_221
ER  - 

@article{
author = "Vicic, Drazen D. and Stoiljković, Milovan and Bojat, Nenad C. and Sabovljevic, Marko S. and Stevanović, Branka M.",
year = "2014",
abstract = "Serpentine (ultramafic) soils are extremely stressful environments for plant growth due to macronutrient deficiency (N, P, K, Ca), macronutrient toxicity (Mg; extremely high Mg:Ca ratio), and micronutrient toxicity (Mn, Fe, Ni, Cu, Zn), as well as toxicity of other heavy metals (Al, Cr, Co). Serpentine soils are also often shallow, rocky, and susceptible to drought. As a result of extreme adverse physical and chemical conditions, serpentine soils support a high proportion of endemic plant species that are adapted to their harsh environment. We analysed root, stem, and leaf tissue element concentrations (Ca, Mg, Al, Mn, Fe, Ni, Cu, and Zn) of Halacsya sendtneri (Boraginaceae; strict serpentine endemic), Cheilanthes marantae (Pteridaceac; broad serpentine endemic/strong indicator), and Seseli rigidum (Apiaccac; weak serpentine indicator/indifferent) growing on serpentine and limestone in Serbia. Element bioaccumulation factor was calculated as the ratio of plant tissue clement concentration to soil plant-available element concentration. Tissue concentrations of Ca and Mg for H. sendtneri and S. rigidum indicate that the species selectively uptake and translocatc Ca to leaves, relative to Mg, to maintain adequate tissue Mg:Ca ratio. C. marantae did not exhibit selective Ca uptake or translocation, but did exhibit Mg sequestration in roots. Heavy metal exclusion and sequestration were the primary physiological tolerance mechanisms conveying serpentine tolerance in the three species. S. rigidum exhibited divergence into serpentine tolerant and limestone tolerant ecotypes, presenting a useful model species for further studies of physiological adaptation to chemically extreme soils.",
journal = "Revue D Ecologie. La Terre et la Vie",
title = "Physiological Tolerance Mechanisms of Serpentine Tolerant Plants from Serbia",
volume = "69",
number = "3-4",
pages = "185-195",
url = "https://hdl.handle.net/21.15107/rcub_vinar_221"
}

Vicic, D. D., Stoiljković, M., Bojat, N. C., Sabovljevic, M. S.,& Stevanović, B. M.. (2014). Physiological Tolerance Mechanisms of Serpentine Tolerant Plants from Serbia. in Revue D Ecologie. La Terre et la Vie, 69(3-4), 185-195.
https://hdl.handle.net/21.15107/rcub_vinar_221

Vicic DD, Stoiljković M, Bojat NC, Sabovljevic MS, Stevanović BM. Physiological Tolerance Mechanisms of Serpentine Tolerant Plants from Serbia. in Revue D Ecologie. La Terre et la Vie. 2014;69(3-4):185-195.
https://hdl.handle.net/21.15107/rcub_vinar_221 .

Vicic, Drazen D., Stoiljković, Milovan, Bojat, Nenad C., Sabovljevic, Marko S., Stevanović, Branka M., "Physiological Tolerance Mechanisms of Serpentine Tolerant Plants from Serbia" in Revue D Ecologie. La Terre et la Vie, 69, no. 3-4 (2014):185-195,
https://hdl.handle.net/21.15107/rcub_vinar_221 .

TY  - JOUR
AU  - Vicic, Drazen D.
AU  - Stoiljković, Milovan
AU  - Ninkov, Jordana M.
AU  - Bojat, Nenad C.
AU  - Sabovljevic, Marko S.
AU  - Stevanović, Branka M.
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/138
AB  - To enhance understanding of edaphic conditions in serpentine habitats, a thorough investigation of the chemical and mechanical properties of three soils from disjunct ultramafic outcrops in the central Balkans was undertaken. Soil from a nearby chemically contrasting limestone habitat was also analyzed. Three plant species differently associated with serpentine habitats (Halacsya sendtneri, Cheilanthes marantae and Seseli rigidum) were references for site and soil selection. Twenty elements were determined, and fourteen were measured in seven sequentially extracted soil fractions. The quantified soil properties included pH, levels of free CaCO3, organic matter, P2O5, K2O, N, C, S, cation exchange capacity, total organic carbon, field capacity and soil mechanical composition. The usual harsh components for plant growth in serpentine soil, such as elevated Mg:Ca ratios, high levels of Ni, Cr or Co, were significantly lower in the available fractions. There was a significant positive correlation of organic matter and field capacity, with most available Ca (70-80 %) found in the mobile, rather than the organically bound fraction.
T2  - Journal of the Serbian Chemical Society
T1  - Dynamics of soil chemistry in different serpentine habitats of Serbia
VL  - 79
IS  - 9
SP  - 1185
EP  - 1198
DO  - 10.2298/JSC130917028V
ER  - 

@article{
author = "Vicic, Drazen D. and Stoiljković, Milovan and Ninkov, Jordana M. and Bojat, Nenad C. and Sabovljevic, Marko S. and Stevanović, Branka M.",
year = "2014",
abstract = "To enhance understanding of edaphic conditions in serpentine habitats, a thorough investigation of the chemical and mechanical properties of three soils from disjunct ultramafic outcrops in the central Balkans was undertaken. Soil from a nearby chemically contrasting limestone habitat was also analyzed. Three plant species differently associated with serpentine habitats (Halacsya sendtneri, Cheilanthes marantae and Seseli rigidum) were references for site and soil selection. Twenty elements were determined, and fourteen were measured in seven sequentially extracted soil fractions. The quantified soil properties included pH, levels of free CaCO3, organic matter, P2O5, K2O, N, C, S, cation exchange capacity, total organic carbon, field capacity and soil mechanical composition. The usual harsh components for plant growth in serpentine soil, such as elevated Mg:Ca ratios, high levels of Ni, Cr or Co, were significantly lower in the available fractions. There was a significant positive correlation of organic matter and field capacity, with most available Ca (70-80 %) found in the mobile, rather than the organically bound fraction.",
journal = "Journal of the Serbian Chemical Society",
title = "Dynamics of soil chemistry in different serpentine habitats of Serbia",
volume = "79",
number = "9",
pages = "1185-1198",
doi = "10.2298/JSC130917028V"
}

Vicic, D. D., Stoiljković, M., Ninkov, J. M., Bojat, N. C., Sabovljevic, M. S.,& Stevanović, B. M.. (2014). Dynamics of soil chemistry in different serpentine habitats of Serbia. in Journal of the Serbian Chemical Society, 79(9), 1185-1198.
https://doi.org/10.2298/JSC130917028V

Vicic DD, Stoiljković M, Ninkov JM, Bojat NC, Sabovljevic MS, Stevanović BM. Dynamics of soil chemistry in different serpentine habitats of Serbia. in Journal of the Serbian Chemical Society. 2014;79(9):1185-1198.
doi:10.2298/JSC130917028V .

Vicic, Drazen D., Stoiljković, Milovan, Ninkov, Jordana M., Bojat, Nenad C., Sabovljevic, Marko S., Stevanović, Branka M., "Dynamics of soil chemistry in different serpentine habitats of Serbia" in Journal of the Serbian Chemical Society, 79, no. 9 (2014):1185-1198,
https://doi.org/10.2298/JSC130917028V . .

TY  - JOUR
AU  - Popovic Bubujuk, Slavica
AU  - Bojat, Nenad C.
AU  - Đelić, Ninoslav
AU  - Dronjak, Slađana
AU  - Kostadinovic, Ljiljana
AU  - Coghill Galonja, Tamara
AU  - Anđelković, Marko
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5852
AB  - Cadmium (Cd) is highly toxic heavy metal which may cause severe biological effects in vivo and in vitro. In this study, an evaluation of the acute Cd ability to trigger micronuclei (MNi) formation was carried out on 3-month-old male and female Albino Oxford (AO) rats using micronucleus (MN) test. Experimental animals were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight. Control animals received equal volume of sterile phosphate buffered saline. The results showed that 2 mg CdCl2 per kg b.w. concentration caused a highly statistically significant (P LT 0.001) increase in MNi formation in the bone marrow polychromatic erythrocytes (PCEs), exerting a clear-cut concentration-dependent effect. Lower concentrations of CdCl2 used (0.5 and 1 mg/kg b.w.) also caused MNi formation, but with lower statistical significance. Sex differences in MNi production in bone marrow PCEs after acute exposure to different experimental concentrations of CdCl2 were not observed in our study. Our results indicate the ability of CdCl2 to exerts genotoxic effects in bone marrow of AO rats, and complement previous data on the genotoxicity of this important environmental contaminant, burdening the body from different sources-major being industrial exposure, food and cigarette smoking.
T2  - Genetika
T1  - The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats
VL  - 45
IS  - 3
SP  - 727
EP  - 736
DO  - 10.2298/GENSR1303727B
ER  - 

@article{
author = "Popovic Bubujuk, Slavica and Bojat, Nenad C. and Đelić, Ninoslav and Dronjak, Slađana and Kostadinovic, Ljiljana and Coghill Galonja, Tamara and Anđelković, Marko",
year = "2013",
abstract = "Cadmium (Cd) is highly toxic heavy metal which may cause severe biological effects in vivo and in vitro. In this study, an evaluation of the acute Cd ability to trigger micronuclei (MNi) formation was carried out on 3-month-old male and female Albino Oxford (AO) rats using micronucleus (MN) test. Experimental animals were treated intraperitoneally with three different concentrations of cadmium chloride (CdCl2): 0.5, 1, and 2 mg CdCl2 per kg of body weight. Control animals received equal volume of sterile phosphate buffered saline. The results showed that 2 mg CdCl2 per kg b.w. concentration caused a highly statistically significant (P LT 0.001) increase in MNi formation in the bone marrow polychromatic erythrocytes (PCEs), exerting a clear-cut concentration-dependent effect. Lower concentrations of CdCl2 used (0.5 and 1 mg/kg b.w.) also caused MNi formation, but with lower statistical significance. Sex differences in MNi production in bone marrow PCEs after acute exposure to different experimental concentrations of CdCl2 were not observed in our study. Our results indicate the ability of CdCl2 to exerts genotoxic effects in bone marrow of AO rats, and complement previous data on the genotoxicity of this important environmental contaminant, burdening the body from different sources-major being industrial exposure, food and cigarette smoking.",
journal = "Genetika",
title = "The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats",
volume = "45",
number = "3",
pages = "727-736",
doi = "10.2298/GENSR1303727B"
}

Popovic Bubujuk, S., Bojat, N. C., Đelić, N., Dronjak, S., Kostadinovic, L., Coghill Galonja, T.,& Anđelković, M.. (2013). The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats. in Genetika, 45(3), 727-736.
https://doi.org/10.2298/GENSR1303727B

Popovic Bubujuk S, Bojat NC, Đelić N, Dronjak S, Kostadinovic L, Coghill Galonja T, Anđelković M. The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats. in Genetika. 2013;45(3):727-736.
doi:10.2298/GENSR1303727B .

Popovic Bubujuk, Slavica, Bojat, Nenad C., Đelić, Ninoslav, Dronjak, Slađana, Kostadinovic, Ljiljana, Coghill Galonja, Tamara, Anđelković, Marko, "The Effect of Different Acute Concentrations of Cadmium Chloride on the Frequency of Micronuclei in Ao Rats" in Genetika, 45, no. 3 (2013):727-736,
https://doi.org/10.2298/GENSR1303727B . .