TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Todorović, Danijela V.
AU  - Petrović, Ivan M.
AU  - Ruzvdijic, S
AU  - Raffaele, L
AU  - Sabini, MG
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Farruggia, G
AU  - Masotti, L
AU  - Kanazir, Dušan T.
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6321
AB  - The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.
T2  - Physica Medica
T1  - Inhibition of human melanoma cell growth by proton irradiation
VL  - 17
SP  - 71
EP  - 75
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6321
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Todorović, Danijela V. and Petrović, Ivan M. and Ruzvdijic, S and Raffaele, L and Sabini, MG and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Farruggia, G and Masotti, L and Kanazir, Dušan T.",
year = "2001",
abstract = "The aim of this work is the in vitro study of human melanoma cell growth modulation after irradiation with protons. Confluent cell monolayers were irradiated with single doses ranging from 1 to 20 Gy, using proton beams having energy of 22.6 MeV at the target. 48 hours after irradiation, cell growth, cell cycle analyses and initiation of cell death were followed. The obtained results were compared with the effects of glucocorticoid hormones. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analyses of melanomas after proton irradiation, have shown the G2/M arrest of irradiated cells corresponding with the increase of applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. Glucocorticoid treatment has shown modest melanoma cell growth inhibition, cell cycle arrest in G2/M phase and ladder pattern on agarose gel electrophoresis.",
journal = "Physica Medica",
title = "Inhibition of human melanoma cell growth by proton irradiation",
volume = "17",
pages = "71-75",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6321"
}

Ristić-Fira, A., Todorović, D. V., Petrović, I. M., Ruzvdijic, S., Raffaele, L., Sabini, M., Cirrone, G. A. P., Cuttone, G., Farruggia, G., Masotti, L.,& Kanazir, D. T.. (2001). Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica, 17, 71-75.
https://hdl.handle.net/21.15107/rcub_vinar_6321

Ristić-Fira A, Todorović DV, Petrović IM, Ruzvdijic S, Raffaele L, Sabini M, Cirrone GAP, Cuttone G, Farruggia G, Masotti L, Kanazir DT. Inhibition of human melanoma cell growth by proton irradiation. in Physica Medica. 2001;17:71-75.
https://hdl.handle.net/21.15107/rcub_vinar_6321 .

Ristić-Fira, Aleksandra, Todorović, Danijela V., Petrović, Ivan M., Ruzvdijic, S, Raffaele, L, Sabini, MG, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Farruggia, G, Masotti, L, Kanazir, Dušan T., "Inhibition of human melanoma cell growth by proton irradiation" in Physica Medica, 17 (2001):71-75,
https://hdl.handle.net/21.15107/rcub_vinar_6321 .

TY  - JOUR
AU  - Ristić-Fira, Aleksandra
AU  - Nikolic, D
AU  - Petrović, Ivan M.
AU  - Ruždijić, Sabera
AU  - Raffaele, L
AU  - Sabini, MG
AU  - Cirrone, Giuseppe Antonio Pablo
AU  - Cuttone, Giacomo
AU  - Farruggia, G
AU  - Masotti, L
AU  - Kanazir, Dušan T.
PY  - 2001
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2426
AB  - The aim of this work is the in vitro study of the late effects of single proton irradiation on HTB63 human melanoma cell growth, cell cycle and cell death. The experimental conditions were focused on analyzing the effects of irradiation on the periphery of tumour that can be, in clinical practice, close to critical organs. Confluent cell monolayers were irradiated with single doses ranging from 1 - 20 Gy, using proton beams having an energy of 22.6 MeV at the target. Antiproliferative effect of protons, cell cycle analysis and initiation of cell death, were followed 48 hours after irradiation. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analysis and cell viability have shown the G2/M and G1/G0 arrest of irradiated cells correlating with the increase of the applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. These data demonstrate that irradiation with protons, under the chosen experimental conditions, have significant effects on melanoma cell growth inhibition being dose dependent, G2/M cell cycle arrest and appearance of apoptotic nuclei, even 48 hours after irradiation. The results obtained may help the understanding of the relationship between cell proliferation, death and cell cycle regulation of melanomas after proton irradiation.
T2  - Journal of Experimental and Clinical Cancer Research
T1  - The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line
VL  - 20
IS  - 1
SP  - 135
EP  - 143
UR  - https://hdl.handle.net/21.15107/rcub_vinar_2426
ER  - 

@article{
author = "Ristić-Fira, Aleksandra and Nikolic, D and Petrović, Ivan M. and Ruždijić, Sabera and Raffaele, L and Sabini, MG and Cirrone, Giuseppe Antonio Pablo and Cuttone, Giacomo and Farruggia, G and Masotti, L and Kanazir, Dušan T.",
year = "2001",
abstract = "The aim of this work is the in vitro study of the late effects of single proton irradiation on HTB63 human melanoma cell growth, cell cycle and cell death. The experimental conditions were focused on analyzing the effects of irradiation on the periphery of tumour that can be, in clinical practice, close to critical organs. Confluent cell monolayers were irradiated with single doses ranging from 1 - 20 Gy, using proton beams having an energy of 22.6 MeV at the target. Antiproliferative effect of protons, cell cycle analysis and initiation of cell death, were followed 48 hours after irradiation. The inhibition of melanoma cell growth was observed, especially after single application of 12 and 16 Gy. Cell cycle analysis and cell viability have shown the G2/M and G1/G0 arrest of irradiated cells correlating with the increase of the applied dose. The flow cytometric analysis has shown presence of apoptotic nuclei. These data demonstrate that irradiation with protons, under the chosen experimental conditions, have significant effects on melanoma cell growth inhibition being dose dependent, G2/M cell cycle arrest and appearance of apoptotic nuclei, even 48 hours after irradiation. The results obtained may help the understanding of the relationship between cell proliferation, death and cell cycle regulation of melanomas after proton irradiation.",
journal = "Journal of Experimental and Clinical Cancer Research",
title = "The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line",
volume = "20",
number = "1",
pages = "135-143",
url = "https://hdl.handle.net/21.15107/rcub_vinar_2426"
}

Ristić-Fira, A., Nikolic, D., Petrović, I. M., Ruždijić, S., Raffaele, L., Sabini, M., Cirrone, G. A. P., Cuttone, G., Farruggia, G., Masotti, L.,& Kanazir, D. T.. (2001). The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line. in Journal of Experimental and Clinical Cancer Research, 20(1), 135-143.
https://hdl.handle.net/21.15107/rcub_vinar_2426

Ristić-Fira A, Nikolic D, Petrović IM, Ruždijić S, Raffaele L, Sabini M, Cirrone GAP, Cuttone G, Farruggia G, Masotti L, Kanazir DT. The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line. in Journal of Experimental and Clinical Cancer Research. 2001;20(1):135-143.
https://hdl.handle.net/21.15107/rcub_vinar_2426 .

Ristić-Fira, Aleksandra, Nikolic, D, Petrović, Ivan M., Ruždijić, Sabera, Raffaele, L, Sabini, MG, Cirrone, Giuseppe Antonio Pablo, Cuttone, Giacomo, Farruggia, G, Masotti, L, Kanazir, Dušan T., "The late effects of proton irradiation on cell growth, cell cycle arrest and apoptosis in a human melanoma cell line" in Journal of Experimental and Clinical Cancer Research, 20, no. 1 (2001):135-143,
https://hdl.handle.net/21.15107/rcub_vinar_2426 .

TY  - JOUR
AU  - Joksić, Gordana
AU  - Pajović, Snežana B.
AU  - Stankovic, M
AU  - Pejić, Snežana
AU  - Kasapović, Jelena
AU  - Cuttone, Giacomo
AU  - Calonghi, N
AU  - Masotti, L
AU  - Kanazir, Dušan T.
PY  - 2000
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/2358
AB  - The goal of this study was to provide data on the dose-dependent production of dicentrics and micronuclei in human lymphocytes irradiated with 22.6 MeV protons and to estimate the possible contribution of intracellular superoxide dismutases (SOD) to the relative biological effectiveness (RBE) of protons. For the dose-response study, heparinized whole blood of a healthy volunteer was irradiated with protons and Xrays employing radiation doses of 0.5-4 Gy. Three biological endpoints were analyzed: chromosomal aberrations, micronuclei, and specific activity of cytosolic (CuZnSOD) and mitochondrial (MnSOD) superoxide dismutases in harvested human blood cells. Dicentric dose-response curves fit a linear-quadratic form (alpha = 0.094 +/- 0.006, beta = 0.032 +/- 0.001) induced with X-rays and (alpha = 0.119 +/- 0.057, beta = 0.029 +/- 0.014) for 22.6 MeV protons. Protons were more effective than X-rays in producing exchanges, particularly at 0.5 and 1 Gy. In contrast to X-ray irradiated samples where a significant increase in the specific activity of MnSOD was recorded (up to a radiation dose of 1 Gy), irradiation with protons markedly reduced its activity. As a consequence of the reduced activity of MnSOD, the chromosomal dose-response curve became quadratic. The RBE for dicentrics varies with dose (from 2.2 to 1.01) and reduced activity of MnSOD is an important contributor to the RBE of protons. SODs, particularly MnSOD, play an important role in defending DNA from reactive oxygen species. A reduced activity of SOD, particularly MnSOD, is an important contributor to the RBE of protons.
T2  - Cellular and Molecular Life Sciences / CMLS
T1  - Chromosome aberrations, micronuclei, and activity of superoxide dismutases in human lymphocytes after irradiation in vitro
VL  - 57
IS  - 5
SP  - 842
EP  - 850
DO  - 10.1007/s000180050046
ER  - 

@article{
author = "Joksić, Gordana and Pajović, Snežana B. and Stankovic, M and Pejić, Snežana and Kasapović, Jelena and Cuttone, Giacomo and Calonghi, N and Masotti, L and Kanazir, Dušan T.",
year = "2000",
abstract = "The goal of this study was to provide data on the dose-dependent production of dicentrics and micronuclei in human lymphocytes irradiated with 22.6 MeV protons and to estimate the possible contribution of intracellular superoxide dismutases (SOD) to the relative biological effectiveness (RBE) of protons. For the dose-response study, heparinized whole blood of a healthy volunteer was irradiated with protons and Xrays employing radiation doses of 0.5-4 Gy. Three biological endpoints were analyzed: chromosomal aberrations, micronuclei, and specific activity of cytosolic (CuZnSOD) and mitochondrial (MnSOD) superoxide dismutases in harvested human blood cells. Dicentric dose-response curves fit a linear-quadratic form (alpha = 0.094 +/- 0.006, beta = 0.032 +/- 0.001) induced with X-rays and (alpha = 0.119 +/- 0.057, beta = 0.029 +/- 0.014) for 22.6 MeV protons. Protons were more effective than X-rays in producing exchanges, particularly at 0.5 and 1 Gy. In contrast to X-ray irradiated samples where a significant increase in the specific activity of MnSOD was recorded (up to a radiation dose of 1 Gy), irradiation with protons markedly reduced its activity. As a consequence of the reduced activity of MnSOD, the chromosomal dose-response curve became quadratic. The RBE for dicentrics varies with dose (from 2.2 to 1.01) and reduced activity of MnSOD is an important contributor to the RBE of protons. SODs, particularly MnSOD, play an important role in defending DNA from reactive oxygen species. A reduced activity of SOD, particularly MnSOD, is an important contributor to the RBE of protons.",
journal = "Cellular and Molecular Life Sciences / CMLS",
title = "Chromosome aberrations, micronuclei, and activity of superoxide dismutases in human lymphocytes after irradiation in vitro",
volume = "57",
number = "5",
pages = "842-850",
doi = "10.1007/s000180050046"
}

Joksić, G., Pajović, S. B., Stankovic, M., Pejić, S., Kasapović, J., Cuttone, G., Calonghi, N., Masotti, L.,& Kanazir, D. T.. (2000). Chromosome aberrations, micronuclei, and activity of superoxide dismutases in human lymphocytes after irradiation in vitro. in Cellular and Molecular Life Sciences / CMLS, 57(5), 842-850.
https://doi.org/10.1007/s000180050046

Joksić G, Pajović SB, Stankovic M, Pejić S, Kasapović J, Cuttone G, Calonghi N, Masotti L, Kanazir DT. Chromosome aberrations, micronuclei, and activity of superoxide dismutases in human lymphocytes after irradiation in vitro. in Cellular and Molecular Life Sciences / CMLS. 2000;57(5):842-850.
doi:10.1007/s000180050046 .

Joksić, Gordana, Pajović, Snežana B., Stankovic, M, Pejić, Snežana, Kasapović, Jelena, Cuttone, Giacomo, Calonghi, N, Masotti, L, Kanazir, Dušan T., "Chromosome aberrations, micronuclei, and activity of superoxide dismutases in human lymphocytes after irradiation in vitro" in Cellular and Molecular Life Sciences / CMLS, 57, no. 5 (2000):842-850,
https://doi.org/10.1007/s000180050046 . .