TY  - JOUR
AU  - Aljancic, Ivana
AU  - Stanković, Miroslava
AU  - Tešević, Vele V.
AU  - Vujisić, Ljubodrag
AU  - Vajs, Vlatka
AU  - Milosavljević, Slobodan
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4002
AB  - This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.
T2  - Natural Product Communications
T1  - Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species
VL  - 5
IS  - 5
SP  - 729
EP  - 732
UR  - https://hdl.handle.net/21.15107/rcub_vinar_4002
ER  - 

@article{
author = "Aljancic, Ivana and Stanković, Miroslava and Tešević, Vele V. and Vujisić, Ljubodrag and Vajs, Vlatka and Milosavljević, Slobodan",
year = "2010",
abstract = "This study was conducted to elucidate the in vitro protective effect of five flavonoids [apigenin (1), apigenin-7-O-glucoside (2), centaureidin (3), jaceidin (4) and quercetin (5)] against chromosomal damage in mitogen-induced human lymphocytes. Using the Cytochalasin-B blocked micronucleus (CBMN) assay, in which the biomarker of chromosome breakage and/or chromosome loss is the elevated frequency of micronucleus (MN) in binucleated (BN) cells, the presence of flavonoid 2 in minimal concentration (3 mu g/mL) gave a 35.5% decrease M the frequency of MN when compared with control human lymphocytes. The same concentration of flavonoids 1, 3 and 4, reduced the MN frequency by 24.4%, 28.0% and 28.0%, respectively. Higher concentrations (6 mu g/mL and 10 mu g/mL) seemed less effective. Flavonoid 5 (3 mu g/mL.) induced a slight decrease in MN frequency (5%), while higher doses (6 mu g/mL and 10 mu g/mL) provoked an increase of DNA damage. The comparable values for the cytokinesis-block proliferation index (CBPI) of the tested flavonoids and positive control suggested an inhibitory effect on lymphocyte proliferation. In the DPPH scavenging assay, flavonoids 1-4 demonstrated modest activity, in a dose-dependent manner, compared with the synthetic antioxidants BHT and Trolox, while 5 exhibited comparably high antioxidative activity.",
journal = "Natural Product Communications",
title = "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species",
volume = "5",
number = "5",
pages = "729-732",
url = "https://hdl.handle.net/21.15107/rcub_vinar_4002"
}

Aljancic, I., Stanković, M., Tešević, V. V., Vujisić, L., Vajs, V.,& Milosavljević, S.. (2010). Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications, 5(5), 729-732.
https://hdl.handle.net/21.15107/rcub_vinar_4002

Aljancic I, Stanković M, Tešević VV, Vujisić L, Vajs V, Milosavljević S. Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species. in Natural Product Communications. 2010;5(5):729-732.
https://hdl.handle.net/21.15107/rcub_vinar_4002 .

Aljancic, Ivana, Stanković, Miroslava, Tešević, Vele V., Vujisić, Ljubodrag, Vajs, Vlatka, Milosavljević, Slobodan, "Protective Effect on Human Lymphocytes of Some Flavonoids Isolated from Two Achillea Species" in Natural Product Communications, 5, no. 5 (2010):729-732,
https://hdl.handle.net/21.15107/rcub_vinar_4002 .

TY  - JOUR
AU  - Lazarević-Pašti, Tamara
AU  - Momić, Tatjana
AU  - Onjia, Antonije E.
AU  - Vujisić, Ljubodrag
AU  - Vasić, Vesna M.
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4092
AB  - In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.
T2  - Microchimica Acta
T1  - Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods
VL  - 170
IS  - 3-4
SP  - 289
EP  - 297
DO  - 10.1007/s00604-010-0324-2
ER  - 

@article{
author = "Lazarević-Pašti, Tamara and Momić, Tatjana and Onjia, Antonije E. and Vujisić, Ljubodrag and Vasić, Vesna M.",
year = "2010",
abstract = "In order to improve the sensitivity of assays for inhibitors of the enzyme acetylcholine esterase (AChE), an effective method was developed for the conversion of the organophosphate pesticides (OPs) diazinon, malathion, chlorpyrifos, azinphos-methyl and phorate into more toxic inhibitors. This was accomplished by converting them from the thio form into their oxo form using the enzyme myeloperoxidase. The oxo forms, which are the only products of conversion, were determined by AChE bioassays, using either the free enzyme, or a flow injection analysis manifold with immobilized AChE and spectrophotometric detection. All modified OPs exhibited inhibitory power at ppb levels and within 10 min. The method is considered to represent an excellent means for improving the sensitivity of assays for determination of OPs.",
journal = "Microchimica Acta",
title = "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods",
volume = "170",
number = "3-4",
pages = "289-297",
doi = "10.1007/s00604-010-0324-2"
}

Lazarević-Pašti, T., Momić, T., Onjia, A. E., Vujisić, L.,& Vasić, V. M.. (2010). Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta, 170(3-4), 289-297.
https://doi.org/10.1007/s00604-010-0324-2

Lazarević-Pašti T, Momić T, Onjia AE, Vujisić L, Vasić VM. Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods. in Microchimica Acta. 2010;170(3-4):289-297.
doi:10.1007/s00604-010-0324-2 .

Lazarević-Pašti, Tamara, Momić, Tatjana, Onjia, Antonije E., Vujisić, Ljubodrag, Vasić, Vesna M., "Myeloperoxidase-mediated oxidation of organophosphorus pesticides as a pre-step in their determination by AChE based bioanalytical methods" in Microchimica Acta, 170, no. 3-4 (2010):289-297,
https://doi.org/10.1007/s00604-010-0324-2 . .

TY  - JOUR
AU  - Tešević, Vele V.
AU  - Nikicevic, Ninoslav
AU  - Milosavljević, Slobodan
AU  - Bajic, Danica
AU  - Vajs, Vlatka
AU  - Vučković, Ivan
AU  - Vujisić, Ljubodrag
AU  - Dordevic, Iris
AU  - Stanković, Miroslava
AU  - Velickovic, Milovan
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3651
AB  - In this study, volatile compounds were analyzed in five samples of home-made spirit beverage made by the distillation of fermented fruits of cornelian cherry (Cornus mas L.). The major volatile compounds, besides ethanol, identified and quantified were: methanol, acetaldehyde, I-propanol, ethyl acetate, 2-methyl-1-propanol, 1-butanol, amyl alcohols, 1-hexanol and 2-phenyl-ethanol, The minor volatiles were submitted to liquid liquid extraction with dichloromethane and analyzed by gas chromatography and gas chromatography/ /mass spectrometry (GC/MS). A total of 84 compounds were identified. The most abundant compounds were straight-chain free fatty acids, ethyl esters of C(6)-C(18) acids, limonene, 2-phenylethanol and 4-ethylphenol. Most of the compounds found in the Drenja spirits investigated in this study arc similar to those present in other alcoholic beverages.
T2  - Journal of the Serbian Chemical Society
T1  - Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry
VL  - 74
IS  - 2
SP  - 117
EP  - 128
DO  - 10.2298/JSC0902117T
ER  - 

@article{
author = "Tešević, Vele V. and Nikicevic, Ninoslav and Milosavljević, Slobodan and Bajic, Danica and Vajs, Vlatka and Vučković, Ivan and Vujisić, Ljubodrag and Dordevic, Iris and Stanković, Miroslava and Velickovic, Milovan",
year = "2009",
abstract = "In this study, volatile compounds were analyzed in five samples of home-made spirit beverage made by the distillation of fermented fruits of cornelian cherry (Cornus mas L.). The major volatile compounds, besides ethanol, identified and quantified were: methanol, acetaldehyde, I-propanol, ethyl acetate, 2-methyl-1-propanol, 1-butanol, amyl alcohols, 1-hexanol and 2-phenyl-ethanol, The minor volatiles were submitted to liquid liquid extraction with dichloromethane and analyzed by gas chromatography and gas chromatography/ /mass spectrometry (GC/MS). A total of 84 compounds were identified. The most abundant compounds were straight-chain free fatty acids, ethyl esters of C(6)-C(18) acids, limonene, 2-phenylethanol and 4-ethylphenol. Most of the compounds found in the Drenja spirits investigated in this study arc similar to those present in other alcoholic beverages.",
journal = "Journal of the Serbian Chemical Society",
title = "Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry",
volume = "74",
number = "2",
pages = "117-128",
doi = "10.2298/JSC0902117T"
}

Tešević, V. V., Nikicevic, N., Milosavljević, S., Bajic, D., Vajs, V., Vučković, I., Vujisić, L., Dordevic, I., Stanković, M.,& Velickovic, M.. (2009). Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry. in Journal of the Serbian Chemical Society, 74(2), 117-128.
https://doi.org/10.2298/JSC0902117T

Tešević VV, Nikicevic N, Milosavljević S, Bajic D, Vajs V, Vučković I, Vujisić L, Dordevic I, Stanković M, Velickovic M. Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry. in Journal of the Serbian Chemical Society. 2009;74(2):117-128.
doi:10.2298/JSC0902117T .

Tešević, Vele V., Nikicevic, Ninoslav, Milosavljević, Slobodan, Bajic, Danica, Vajs, Vlatka, Vučković, Ivan, Vujisić, Ljubodrag, Dordevic, Iris, Stanković, Miroslava, Velickovic, Milovan, "Characterization of volatile compounds of Drenja, an alcoholic beverage obtained from the fruits of cornelian cherry" in Journal of the Serbian Chemical Society, 74, no. 2 (2009):117-128,
https://doi.org/10.2298/JSC0902117T . .