TY  - JOUR
AU  - Lavtar, Polona
AU  - Rudolf, Gorazd
AU  - Maver, Aleš
AU  - Hodžić, Alenka
AU  - Starčević Čizmarević, Nada
AU  - Živković, Maja
AU  - Jazbec, Sasa Sega
AU  - Klemenc-Ketiš, Zalika
AU  - Kapović, Miljenko
AU  - Dinčić, Evica
AU  - Raičević, Ranko
AU  - Sepčić, Juraj
AU  - Lovrečić, Luca
AU  - Stanković, Aleksandra
AU  - Ristić, Smiljana
AU  - Peterlin, Borut
PY  - 2018
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1913
AB  - Prevalence of multiple sclerosis varies with geographic latitude. We hypothesized that this fact might be partially associated with the influence of latitude on circadian rhythm and consequently that genetic variability of key circadian rhythm regulators, ARNTL and CLOCK genes, might contribute to the risk for multiple sclerosis. Our aim was to analyse selected polymorphisms of ARNTL and CLOCK, and their association with multiple sclerosis. A total of 900 Caucasian patients and 1024 healthy controls were compared for genetic signature at 8 SNPs, 4 for each of both genes. We found a statistically significant difference in genotype (ARNTL rs3789327, P = 7.5.10(-5); CLOCK rs6811520 P = 0.02) distributions in patients and controls. The ARNTL rs3789327 CC genotype was associated with higher risk for multiple sclerosis at an OR of 1.67 (95% CI 1.35-2.07, P = 0.0001) and the CLOCK rs6811520 genotype CC at an OR of 1.40 (95% CI 1.13-1.73, P = 0.002). The results of this study suggest that genetic variability in the ARNTL and CLOCK genes might be associated with risk for multiple sclerosis.
T2  - PLOS One
T1  - Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis
VL  - 13
IS  - 1
SP  - e0190601
DO  - 10.1371/journal.pone.0190601
ER  - 

@article{
author = "Lavtar, Polona and Rudolf, Gorazd and Maver, Aleš and Hodžić, Alenka and Starčević Čizmarević, Nada and Živković, Maja and Jazbec, Sasa Sega and Klemenc-Ketiš, Zalika and Kapović, Miljenko and Dinčić, Evica and Raičević, Ranko and Sepčić, Juraj and Lovrečić, Luca and Stanković, Aleksandra and Ristić, Smiljana and Peterlin, Borut",
year = "2018",
abstract = "Prevalence of multiple sclerosis varies with geographic latitude. We hypothesized that this fact might be partially associated with the influence of latitude on circadian rhythm and consequently that genetic variability of key circadian rhythm regulators, ARNTL and CLOCK genes, might contribute to the risk for multiple sclerosis. Our aim was to analyse selected polymorphisms of ARNTL and CLOCK, and their association with multiple sclerosis. A total of 900 Caucasian patients and 1024 healthy controls were compared for genetic signature at 8 SNPs, 4 for each of both genes. We found a statistically significant difference in genotype (ARNTL rs3789327, P = 7.5.10(-5); CLOCK rs6811520 P = 0.02) distributions in patients and controls. The ARNTL rs3789327 CC genotype was associated with higher risk for multiple sclerosis at an OR of 1.67 (95% CI 1.35-2.07, P = 0.0001) and the CLOCK rs6811520 genotype CC at an OR of 1.40 (95% CI 1.13-1.73, P = 0.002). The results of this study suggest that genetic variability in the ARNTL and CLOCK genes might be associated with risk for multiple sclerosis.",
journal = "PLOS One",
title = "Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis",
volume = "13",
number = "1",
pages = "e0190601",
doi = "10.1371/journal.pone.0190601"
}

Lavtar, P., Rudolf, G., Maver, A., Hodžić, A., Starčević Čizmarević, N., Živković, M., Jazbec, S. S., Klemenc-Ketiš, Z., Kapović, M., Dinčić, E., Raičević, R., Sepčić, J., Lovrečić, L., Stanković, A., Ristić, S.,& Peterlin, B.. (2018). Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis. in PLOS One, 13(1), e0190601.
https://doi.org/10.1371/journal.pone.0190601

Lavtar P, Rudolf G, Maver A, Hodžić A, Starčević Čizmarević N, Živković M, Jazbec SS, Klemenc-Ketiš Z, Kapović M, Dinčić E, Raičević R, Sepčić J, Lovrečić L, Stanković A, Ristić S, Peterlin B. Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis. in PLOS One. 2018;13(1):e0190601.
doi:10.1371/journal.pone.0190601 .

Lavtar, Polona, Rudolf, Gorazd, Maver, Aleš, Hodžić, Alenka, Starčević Čizmarević, Nada, Živković, Maja, Jazbec, Sasa Sega, Klemenc-Ketiš, Zalika, Kapović, Miljenko, Dinčić, Evica, Raičević, Ranko, Sepčić, Juraj, Lovrečić, Luca, Stanković, Aleksandra, Ristić, Smiljana, Peterlin, Borut, "Association of circadian rhythm genes ARNTL/BMAL1 and CLOCK with multiple sclerosis" in PLOS One, 13, no. 1 (2018):e0190601,
https://doi.org/10.1371/journal.pone.0190601 . .

TY  - JOUR
AU  - Kostic, Smiljana
AU  - Kolić, Ivana
AU  - Raičević, Ranko
AU  - Stojanovic, Zvezdana
AU  - Kostić, Dejan
AU  - Dinčić, Evica
PY  - 2017
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/1626
AB  - Background/Aim. Due to the fact that there is a relatively small number of data related to systemic insulin abnormalities in the multiple sclerosis (MS), the main objective of our study was to determine whether a dysbalance of glucose and insulin metabolism exist in patients with natural course of MS. Our hypothesis was that the metabolic disorder that characterizes state of the insulin resistance (IR) and reduced insulin sensitivity (IS) in untreated patients with MS could play a role in disease progression and degree of functional disability. Methods. The study included 31 patients with relapsing-remitting (RR) MS and 14 healthy controls from the same geographic area matched by age, ethnicity and number of smokers. The glucose tolerance, IS, and IR were examined using an oral glucose tolerance test (OGTT) and using basal plasma glucose and insulin levels. The functional disability and disease progression were evaluated by the Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS). Results. The MS patients tolerated glucose equally well as the healthy controls. Basal concentrations of insulin were significantly higher in the MS group p LT 0.05), as well as insulin plasma level 30 min after oral glucose load (p LT 0.01). The patients with MS had significantly higher values of homeostasis model assessment indexes of IR (HOMA-IR) (p = 0.027; p = 0.028). The percentage of IS (HOMA2 % S) and whole body IS index (ISI Matsuda) showed significantly lower values in the MS patients than in the controls (p = 0.005; p = 0.001). The insulinogenic index in the first 30 min of OGTT was significantly higher in MS patients (p = 0.005). The measures of functional disability and MS progression did not correlate significantly with the investigated parameters of IR and IS indexes. Conclusion. This study demonstrates for the first time the existence of hyperinsulinemia, reduced insulin sensitivity and normal glucose tolerance that indicate the initial phase of IR in the natural course of MS. Additional research is necessary in order to define the mechanisms of occurrence and the impact of IR on the complex pathophysiological processes in MS.
T2  - Vojnosanitetski pregled
T1  - Insulin resistance in drug naive patients with multiple sclerosis
VL  - 74
IS  - 6
SP  - 563
EP  - 570
DO  - 10.2298/VSP160218082K
ER  - 

@article{
author = "Kostic, Smiljana and Kolić, Ivana and Raičević, Ranko and Stojanovic, Zvezdana and Kostić, Dejan and Dinčić, Evica",
year = "2017",
abstract = "Background/Aim. Due to the fact that there is a relatively small number of data related to systemic insulin abnormalities in the multiple sclerosis (MS), the main objective of our study was to determine whether a dysbalance of glucose and insulin metabolism exist in patients with natural course of MS. Our hypothesis was that the metabolic disorder that characterizes state of the insulin resistance (IR) and reduced insulin sensitivity (IS) in untreated patients with MS could play a role in disease progression and degree of functional disability. Methods. The study included 31 patients with relapsing-remitting (RR) MS and 14 healthy controls from the same geographic area matched by age, ethnicity and number of smokers. The glucose tolerance, IS, and IR were examined using an oral glucose tolerance test (OGTT) and using basal plasma glucose and insulin levels. The functional disability and disease progression were evaluated by the Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS). Results. The MS patients tolerated glucose equally well as the healthy controls. Basal concentrations of insulin were significantly higher in the MS group p LT 0.05), as well as insulin plasma level 30 min after oral glucose load (p LT 0.01). The patients with MS had significantly higher values of homeostasis model assessment indexes of IR (HOMA-IR) (p = 0.027; p = 0.028). The percentage of IS (HOMA2 % S) and whole body IS index (ISI Matsuda) showed significantly lower values in the MS patients than in the controls (p = 0.005; p = 0.001). The insulinogenic index in the first 30 min of OGTT was significantly higher in MS patients (p = 0.005). The measures of functional disability and MS progression did not correlate significantly with the investigated parameters of IR and IS indexes. Conclusion. This study demonstrates for the first time the existence of hyperinsulinemia, reduced insulin sensitivity and normal glucose tolerance that indicate the initial phase of IR in the natural course of MS. Additional research is necessary in order to define the mechanisms of occurrence and the impact of IR on the complex pathophysiological processes in MS.",
journal = "Vojnosanitetski pregled",
title = "Insulin resistance in drug naive patients with multiple sclerosis",
volume = "74",
number = "6",
pages = "563-570",
doi = "10.2298/VSP160218082K"
}

Kostic, S., Kolić, I., Raičević, R., Stojanovic, Z., Kostić, D.,& Dinčić, E.. (2017). Insulin resistance in drug naive patients with multiple sclerosis. in Vojnosanitetski pregled, 74(6), 563-570.
https://doi.org/10.2298/VSP160218082K

Kostic S, Kolić I, Raičević R, Stojanovic Z, Kostić D, Dinčić E. Insulin resistance in drug naive patients with multiple sclerosis. in Vojnosanitetski pregled. 2017;74(6):563-570.
doi:10.2298/VSP160218082K .

Kostic, Smiljana, Kolić, Ivana, Raičević, Ranko, Stojanovic, Zvezdana, Kostić, Dejan, Dinčić, Evica, "Insulin resistance in drug naive patients with multiple sclerosis" in Vojnosanitetski pregled, 74, no. 6 (2017):563-570,
https://doi.org/10.2298/VSP160218082K . .

TY  - JOUR
AU  - Krsmanovic, Zeljko
AU  - Živković, Maja
AU  - Lepic, Toplica
AU  - Stanković, Aleksandra
AU  - Raičević, Ranko
AU  - Dinčić, Evica
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5618
AB  - Background: Recent evidence has indicated an association between chronic cerebrospinal venous insufficiency (CCSVI) and multiple sclerosis. Small internal jugular veins (IJVs) (with a cross-sectional area of less than 0.4 cm(2)) have been previously described as difficult to catheterize, and their presence may potentially affect cerebrospinal venous drainage. In this blinded extracranial color-Doppler study we had two principal aims: first, to assess prevalence of CCSVI among Serbian MS patients compared to healthy controls; and second, to assess prevalence of small IJVs (with a CSA LT = 0.4 cm(2)) among MS patients and controls. Methods: The sixty seven unrelated patients with clinical isolated syndrome (CIS), relapsing-remitting (RR), secondary progressive (SP) and primary progressive (PP) multiple sclerosis and 21 healthy controls were examined by high-resolution color-Doppler. Results: The ultrasonographic criteria of CCSVI (according to Zamboni) were positive in 11.9% of the patients and in none of the control subjects. The CCSVI-positive patients had significantly longer disease durations and were significantly more disabled (measured by their Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS) scores), but after adjustment for gender and disease duration, CCSVI was not an independent risk factor for multiple sclerosis severity. The small IJVs were found in 28.4% of the patients and 28.6% of the controls. The patients with small IJVs were associated with decreased venous outflow from the brain and presented with longer disease durations and significantly higher EDSS and MSSS scores compared to patients without small IJVs. A multivariate logistic regression analysis adjusted for gender and disease duration showed that small IJV is an independent factor associated with multiple sclerosis severity (EDSS GT = 6) (adjusted OR = 8.9, 95% CI: 1.8-45.6, p = 0.007). Among patients with small IJVs the 36.84% were also CCSVI positive. Conclusions: Both, CCSVI and small IJVs seem to influence or follow MS severity, but only small IJVs turned out to be an independent factor in this study. Thus, small IJVs with restricted outflow, which might be aspects of CCSVI different from the criteria originally described by Zamboni, emerge as a cofactor in the multifactorial pathophysiology of multiple sclerosis.
T2  - BMC Neurology
T1  - Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study
VL  - 13
DO  - 10.1186/1471-2377-13-90
ER  - 

@article{
author = "Krsmanovic, Zeljko and Živković, Maja and Lepic, Toplica and Stanković, Aleksandra and Raičević, Ranko and Dinčić, Evica",
year = "2013",
abstract = "Background: Recent evidence has indicated an association between chronic cerebrospinal venous insufficiency (CCSVI) and multiple sclerosis. Small internal jugular veins (IJVs) (with a cross-sectional area of less than 0.4 cm(2)) have been previously described as difficult to catheterize, and their presence may potentially affect cerebrospinal venous drainage. In this blinded extracranial color-Doppler study we had two principal aims: first, to assess prevalence of CCSVI among Serbian MS patients compared to healthy controls; and second, to assess prevalence of small IJVs (with a CSA LT = 0.4 cm(2)) among MS patients and controls. Methods: The sixty seven unrelated patients with clinical isolated syndrome (CIS), relapsing-remitting (RR), secondary progressive (SP) and primary progressive (PP) multiple sclerosis and 21 healthy controls were examined by high-resolution color-Doppler. Results: The ultrasonographic criteria of CCSVI (according to Zamboni) were positive in 11.9% of the patients and in none of the control subjects. The CCSVI-positive patients had significantly longer disease durations and were significantly more disabled (measured by their Expanded Disability Status Scale (EDSS) and Multiple Sclerosis Severity Score (MSSS) scores), but after adjustment for gender and disease duration, CCSVI was not an independent risk factor for multiple sclerosis severity. The small IJVs were found in 28.4% of the patients and 28.6% of the controls. The patients with small IJVs were associated with decreased venous outflow from the brain and presented with longer disease durations and significantly higher EDSS and MSSS scores compared to patients without small IJVs. A multivariate logistic regression analysis adjusted for gender and disease duration showed that small IJV is an independent factor associated with multiple sclerosis severity (EDSS GT = 6) (adjusted OR = 8.9, 95% CI: 1.8-45.6, p = 0.007). Among patients with small IJVs the 36.84% were also CCSVI positive. Conclusions: Both, CCSVI and small IJVs seem to influence or follow MS severity, but only small IJVs turned out to be an independent factor in this study. Thus, small IJVs with restricted outflow, which might be aspects of CCSVI different from the criteria originally described by Zamboni, emerge as a cofactor in the multifactorial pathophysiology of multiple sclerosis.",
journal = "BMC Neurology",
title = "Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study",
volume = "13",
doi = "10.1186/1471-2377-13-90"
}

Krsmanovic, Z., Živković, M., Lepic, T., Stanković, A., Raičević, R.,& Dinčić, E.. (2013). Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study. in BMC Neurology, 13.
https://doi.org/10.1186/1471-2377-13-90

Krsmanovic Z, Živković M, Lepic T, Stanković A, Raičević R, Dinčić E. Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study. in BMC Neurology. 2013;13.
doi:10.1186/1471-2377-13-90 .

Krsmanovic, Zeljko, Živković, Maja, Lepic, Toplica, Stanković, Aleksandra, Raičević, Ranko, Dinčić, Evica, "Small internal jugular veins with restricted outflow are associated with severe multiple sclerosis: a sonographer-blinded, case-control ultrasound study" in BMC Neurology, 13 (2013),
https://doi.org/10.1186/1471-2377-13-90 . .

TY  - JOUR
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Ristić, Smiljana
AU  - Lovrečić, Luca
AU  - Starčević Čizmarević, Nada
AU  - Đurić, Tamara
AU  - Sepčić, Juraj
AU  - Kapović, Miljenko
AU  - Raičević, Ranko
AU  - Peterlin, Borut
AU  - Alavantić, Dragan
AU  - Živković, Maja
PY  - 2010
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4004
AB  - The interleukin 7 receptor alpha single nucleotide polymorphism rs6897932 was identified as a multiple sclerosis susceptibility-modifying polymorphism in genome-wide and gene scan studies, mainly in populations in western countries. The aim of this study was to investigate the association of interleukin 7 receptor alpha rs6897932 with multiple sclerosis in populations from the Western Balkans: Serbia, Croatia, and Slovenia. A total of 678 unrelated white patients and 597 unrelated, ethnically matched healthy controls were included in the study. Genotyping was performed by real-time polymerase chain reaction. We found no significant difference in genotype or allele frequencies between controls and patients with multiple sclerosis either separately in Serbian, Croatian, and Slovenian populations or in the whole sample from the Western Balkans. The odds ratio for multiple sclerosis in this study was 1.04 (0.86-1.25) for the C allele. It is known that demographic as well as environmental factors have a substantial role in multiple sclerosis development, as well as population genetic background. The results of this study indicate that other types of genome variants should be required for the development and/or progression of multiple sclerosis, which may vary among populations.
T2  - Multiple Sclerosis Journal
T1  - Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans
VL  - 16
IS  - 5
SP  - 533
EP  - 536
DO  - 10.1177/1352458509360548
ER  - 

@article{
author = "Stanković, Aleksandra and Dinčić, Evica and Ristić, Smiljana and Lovrečić, Luca and Starčević Čizmarević, Nada and Đurić, Tamara and Sepčić, Juraj and Kapović, Miljenko and Raičević, Ranko and Peterlin, Borut and Alavantić, Dragan and Živković, Maja",
year = "2010",
abstract = "The interleukin 7 receptor alpha single nucleotide polymorphism rs6897932 was identified as a multiple sclerosis susceptibility-modifying polymorphism in genome-wide and gene scan studies, mainly in populations in western countries. The aim of this study was to investigate the association of interleukin 7 receptor alpha rs6897932 with multiple sclerosis in populations from the Western Balkans: Serbia, Croatia, and Slovenia. A total of 678 unrelated white patients and 597 unrelated, ethnically matched healthy controls were included in the study. Genotyping was performed by real-time polymerase chain reaction. We found no significant difference in genotype or allele frequencies between controls and patients with multiple sclerosis either separately in Serbian, Croatian, and Slovenian populations or in the whole sample from the Western Balkans. The odds ratio for multiple sclerosis in this study was 1.04 (0.86-1.25) for the C allele. It is known that demographic as well as environmental factors have a substantial role in multiple sclerosis development, as well as population genetic background. The results of this study indicate that other types of genome variants should be required for the development and/or progression of multiple sclerosis, which may vary among populations.",
journal = "Multiple Sclerosis Journal",
title = "Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans",
volume = "16",
number = "5",
pages = "533-536",
doi = "10.1177/1352458509360548"
}

Stanković, A., Dinčić, E., Ristić, S., Lovrečić, L., Starčević Čizmarević, N., Đurić, T., Sepčić, J., Kapović, M., Raičević, R., Peterlin, B., Alavantić, D.,& Živković, M.. (2010). Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans. in Multiple Sclerosis Journal, 16(5), 533-536.
https://doi.org/10.1177/1352458509360548

Stanković A, Dinčić E, Ristić S, Lovrečić L, Starčević Čizmarević N, Đurić T, Sepčić J, Kapović M, Raičević R, Peterlin B, Alavantić D, Živković M. Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans. in Multiple Sclerosis Journal. 2010;16(5):533-536.
doi:10.1177/1352458509360548 .

Stanković, Aleksandra, Dinčić, Evica, Ristić, Smiljana, Lovrečić, Luca, Starčević Čizmarević, Nada, Đurić, Tamara, Sepčić, Juraj, Kapović, Miljenko, Raičević, Ranko, Peterlin, Borut, Alavantić, Dragan, Živković, Maja, "Interleukin 7 receptor alpha polymorphism rs6897932 and susceptibility to multiple sclerosis in the Western Balkans" in Multiple Sclerosis Journal, 16, no. 5 (2010):533-536,
https://doi.org/10.1177/1352458509360548 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Dinčić, Evica
AU  - Popović, Milan
AU  - Popovic, Smiljana
AU  - Raičević, Ranko
AU  - Alavantić, Dragan
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3771
AB  - Background: Recently a high-resolution HLA and SNP map was defined and the analysis provided informative tag SNPs that capture much of the common variation in the MHC region. This concept enables detection of smaller number of SNPs, making it surrogate markers for haplotype associated with certain disease. The SNP rs3135388 was proposed as a tagging SNP for DRB1*1501/DQB1*0602 alleles, associated with MS. The aim of the study was to investigate the HLA rs3135388 genotypes in association with MS in patients from Serbia. Methods: Two hundred sixty nine consecutive patients from Serbia with relapse-remitting and secondary progressive MS were recruited for the study. Genomic DNA was isolated from peripheral blood cells. We designed the TaqMan assay for high-throughput genotyping of HLA rs3135388 on 7500 Real-Time PCR System. Results: We found significantly higher frequency of rs3135388 A allele carriers in MS patients compared to controls (p LT 0.001, chi(2)). In our population the carriers of one A allele had adjusted OR 2.09 (95% CI 1.41-3.09, p LT 0.001) for MS susceptibility. Conclusion: We assessed significant association of rs3135388 A allele carriership with MS in patients from Serbia. This HLA-DRB1*1501 surrogate marker is useful in association studies in MS. (C) 2009 Elsevier B.V. All rights reserved.
T2  - Clinica Chimica Acta
T1  - The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR
VL  - 406
IS  - 1-2
SP  - 27
EP  - 30
DO  - 10.1016/j.cca.2009.05.004
ER  - 

@article{
author = "Živković, Maja and Stanković, Aleksandra and Dinčić, Evica and Popović, Milan and Popovic, Smiljana and Raičević, Ranko and Alavantić, Dragan",
year = "2009",
abstract = "Background: Recently a high-resolution HLA and SNP map was defined and the analysis provided informative tag SNPs that capture much of the common variation in the MHC region. This concept enables detection of smaller number of SNPs, making it surrogate markers for haplotype associated with certain disease. The SNP rs3135388 was proposed as a tagging SNP for DRB1*1501/DQB1*0602 alleles, associated with MS. The aim of the study was to investigate the HLA rs3135388 genotypes in association with MS in patients from Serbia. Methods: Two hundred sixty nine consecutive patients from Serbia with relapse-remitting and secondary progressive MS were recruited for the study. Genomic DNA was isolated from peripheral blood cells. We designed the TaqMan assay for high-throughput genotyping of HLA rs3135388 on 7500 Real-Time PCR System. Results: We found significantly higher frequency of rs3135388 A allele carriers in MS patients compared to controls (p LT 0.001, chi(2)). In our population the carriers of one A allele had adjusted OR 2.09 (95% CI 1.41-3.09, p LT 0.001) for MS susceptibility. Conclusion: We assessed significant association of rs3135388 A allele carriership with MS in patients from Serbia. This HLA-DRB1*1501 surrogate marker is useful in association studies in MS. (C) 2009 Elsevier B.V. All rights reserved.",
journal = "Clinica Chimica Acta",
title = "The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR",
volume = "406",
number = "1-2",
pages = "27-30",
doi = "10.1016/j.cca.2009.05.004"
}

Živković, M., Stanković, A., Dinčić, E., Popović, M., Popovic, S., Raičević, R.,& Alavantić, D.. (2009). The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR. in Clinica Chimica Acta, 406(1-2), 27-30.
https://doi.org/10.1016/j.cca.2009.05.004

Živković M, Stanković A, Dinčić E, Popović M, Popovic S, Raičević R, Alavantić D. The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR. in Clinica Chimica Acta. 2009;406(1-2):27-30.
doi:10.1016/j.cca.2009.05.004 .

Živković, Maja, Stanković, Aleksandra, Dinčić, Evica, Popović, Milan, Popovic, Smiljana, Raičević, Ranko, Alavantić, Dragan, "The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR" in Clinica Chimica Acta, 406, no. 1-2 (2009):27-30,
https://doi.org/10.1016/j.cca.2009.05.004 . .

TY  - JOUR
AU  - Đurić, Tamara
AU  - Živković, Maja
AU  - Radak, Đorđe J.
AU  - Jekić, Đole
AU  - Radak, Sandra
AU  - Stojković, Ljiljana S.
AU  - Raičević, Ranko
AU  - Stanković, Aleksandra
AU  - Alavantić, Dragan
PY  - 2008
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3571
AB  - Objectives: Stromelysin-1 (MMP-3) as a key member of metalloproteinase family could have an important role in atherogenesis. The 5A/6A polymorphism in the promoter of MMP-3 gene affects the level of MMP-3 gene expression. We assessed whether the MMP-3 promoter low-and high-activity genotypes are related to susceptibility for carotid atherosclerosis (CA) in Serbian population. Design and methods: The study group of case-control design consisted of 515 participants. The 265 patients with ultrasonographic evidence of carotid plaque presence were recruited for the study. The 5A/6A polymorphism was typed by RFLP-PCR. Results: There was significantly higher prevalence of genotypes containing 6A allele in the patients with CA compared to controls (p LT 0.05). The model of inheritance with the dominant effect of 6A allele gave elevated and significant OR for carotid atherosclerosis (adjusted OR 2.35, CI = 1.0-5.5, p=0.048). Conclusions: Subjects carrying genotypes with 6A allele had significantly higher susceptibility to carotid atherosclerosis. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.
T2  - Clinical Biochemistry
T1  - Association of MMP-3 5A/6A gene polymorphism with susceptibility to carotid atherosclerosis
VL  - 41
IS  - 16-17
SP  - 1326
EP  - 1329
DO  - 10.1016/j.clinbiochem.2008.08.081
ER  - 

@article{
author = "Đurić, Tamara and Živković, Maja and Radak, Đorđe J. and Jekić, Đole and Radak, Sandra and Stojković, Ljiljana S. and Raičević, Ranko and Stanković, Aleksandra and Alavantić, Dragan",
year = "2008",
abstract = "Objectives: Stromelysin-1 (MMP-3) as a key member of metalloproteinase family could have an important role in atherogenesis. The 5A/6A polymorphism in the promoter of MMP-3 gene affects the level of MMP-3 gene expression. We assessed whether the MMP-3 promoter low-and high-activity genotypes are related to susceptibility for carotid atherosclerosis (CA) in Serbian population. Design and methods: The study group of case-control design consisted of 515 participants. The 265 patients with ultrasonographic evidence of carotid plaque presence were recruited for the study. The 5A/6A polymorphism was typed by RFLP-PCR. Results: There was significantly higher prevalence of genotypes containing 6A allele in the patients with CA compared to controls (p LT 0.05). The model of inheritance with the dominant effect of 6A allele gave elevated and significant OR for carotid atherosclerosis (adjusted OR 2.35, CI = 1.0-5.5, p=0.048). Conclusions: Subjects carrying genotypes with 6A allele had significantly higher susceptibility to carotid atherosclerosis. (C) 2008 The Canadian Society of Clinical Chemists. Published by Elsevier Inc. All rights reserved.",
journal = "Clinical Biochemistry",
title = "Association of MMP-3 5A/6A gene polymorphism with susceptibility to carotid atherosclerosis",
volume = "41",
number = "16-17",
pages = "1326-1329",
doi = "10.1016/j.clinbiochem.2008.08.081"
}

Đurić, T., Živković, M., Radak, Đ. J., Jekić, Đ., Radak, S., Stojković, L. S., Raičević, R., Stanković, A.,& Alavantić, D.. (2008). Association of MMP-3 5A/6A gene polymorphism with susceptibility to carotid atherosclerosis. in Clinical Biochemistry, 41(16-17), 1326-1329.
https://doi.org/10.1016/j.clinbiochem.2008.08.081

Đurić T, Živković M, Radak ĐJ, Jekić Đ, Radak S, Stojković LS, Raičević R, Stanković A, Alavantić D. Association of MMP-3 5A/6A gene polymorphism with susceptibility to carotid atherosclerosis. in Clinical Biochemistry. 2008;41(16-17):1326-1329.
doi:10.1016/j.clinbiochem.2008.08.081 .

Đurić, Tamara, Živković, Maja, Radak, Đorđe J., Jekić, Đole, Radak, Sandra, Stojković, Ljiljana S., Raičević, Ranko, Stanković, Aleksandra, Alavantić, Dragan, "Association of MMP-3 5A/6A gene polymorphism with susceptibility to carotid atherosclerosis" in Clinical Biochemistry, 41, no. 16-17 (2008):1326-1329,
https://doi.org/10.1016/j.clinbiochem.2008.08.081 . .

TY  - JOUR
AU  - Živković, Maja
AU  - Đurić, Tamara
AU  - Dinčić, Evica
AU  - Raičević, Ranko
AU  - Alavantić, Dragan
AU  - Stanković, Aleksandra
PY  - 2007
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3299
AB  - Matrix metalloproteinase-9 (MMP-9) is suggested to play a role in MS by mediating T cell migration across subendothelial basement membrane and by contribution to myelin breakdown. We studied the association of MMP-9 -1562 C/T gene polymorphisms with MS susceptibility and severity in 187 patients from Serbia. The significant decrease in T allele carriership (p=0.01), was found in female NIS patients, In addition, a trend toward lower MSSS in T allele carriers was noticed (CC, mean 5.7 +/- 2.5 vs. CT+TT, mean 4.9 +/- 2.5). Further studies in different populations are needed to resolve the potential influence of MMP-9 gene polymorphism on MS. (C) 2007 Elsevier B.V. All rights reserved.
T2  - Journal of Neuroimmunology
T1  - Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis
VL  - 189
IS  - 1-2
SP  - 147
EP  - 150
DO  - 10.1016/j.jneuroim.2007.06.022
ER  - 

@article{
author = "Živković, Maja and Đurić, Tamara and Dinčić, Evica and Raičević, Ranko and Alavantić, Dragan and Stanković, Aleksandra",
year = "2007",
abstract = "Matrix metalloproteinase-9 (MMP-9) is suggested to play a role in MS by mediating T cell migration across subendothelial basement membrane and by contribution to myelin breakdown. We studied the association of MMP-9 -1562 C/T gene polymorphisms with MS susceptibility and severity in 187 patients from Serbia. The significant decrease in T allele carriership (p=0.01), was found in female NIS patients, In addition, a trend toward lower MSSS in T allele carriers was noticed (CC, mean 5.7 +/- 2.5 vs. CT+TT, mean 4.9 +/- 2.5). Further studies in different populations are needed to resolve the potential influence of MMP-9 gene polymorphism on MS. (C) 2007 Elsevier B.V. All rights reserved.",
journal = "Journal of Neuroimmunology",
title = "Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis",
volume = "189",
number = "1-2",
pages = "147-150",
doi = "10.1016/j.jneuroim.2007.06.022"
}

Živković, M., Đurić, T., Dinčić, E., Raičević, R., Alavantić, D.,& Stanković, A.. (2007). Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis. in Journal of Neuroimmunology, 189(1-2), 147-150.
https://doi.org/10.1016/j.jneuroim.2007.06.022

Živković M, Đurić T, Dinčić E, Raičević R, Alavantić D, Stanković A. Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis. in Journal of Neuroimmunology. 2007;189(1-2):147-150.
doi:10.1016/j.jneuroim.2007.06.022 .

Živković, Maja, Đurić, Tamara, Dinčić, Evica, Raičević, Ranko, Alavantić, Dragan, Stanković, Aleksandra, "Matrix metalloproteinase-9-1562 C/T gene polymorphism in Serbian patients with multiple sclerosis" in Journal of Neuroimmunology, 189, no. 1-2 (2007):147-150,
https://doi.org/10.1016/j.jneuroim.2007.06.022 . .

TY  - JOUR
AU  - Dinčić, Evica
AU  - Živković, Maja
AU  - Stanković, Aleksandra
AU  - Obradović, Dragana
AU  - Alavantić, Dragan
AU  - Kostic, Vladimir
AU  - Raičević, Ranko
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3078
AB  - We have investigated separate as well as combined influence of IL-1 beta TaqI, IL-1ra VNTR and CTLA-4 +49 A/G polymorphisms on susceptibility, clinical course and progression of MS in 162 Serbian patients. We found significant independent relative risk for MS susceptibility in noncarriers of IL-Ira allele 2 (OR=2.2, CI= 1.3-3.7, p=0.003) and CTLA-4 +49 AA genotype (OR=2.0, CI=1.2-3.5, p=0.01) as well as their combined effect (OR=4.4, CI=2.0-9.7, p=0.0003). Our result supports the significant and combined effect of IL-1ra VNTR and CTLA-4 polymorphisms on MS justifying the need for further haplotype analysis in different populations. (c) 2006 Elsevier B.V. All rights reserved.
T2  - Journal of Neuroimmunology
T1  - Association of polymorphisms in CTLA-4, IL-1ra and IL-1 beta genes with multiple sclerosis in Serbian population
VL  - 177
IS  - 1-2
SP  - 146
EP  - 150
DO  - 10.1016/j.jneuroim.2006.05.005
ER  - 

@article{
author = "Dinčić, Evica and Živković, Maja and Stanković, Aleksandra and Obradović, Dragana and Alavantić, Dragan and Kostic, Vladimir and Raičević, Ranko",
year = "2006",
abstract = "We have investigated separate as well as combined influence of IL-1 beta TaqI, IL-1ra VNTR and CTLA-4 +49 A/G polymorphisms on susceptibility, clinical course and progression of MS in 162 Serbian patients. We found significant independent relative risk for MS susceptibility in noncarriers of IL-Ira allele 2 (OR=2.2, CI= 1.3-3.7, p=0.003) and CTLA-4 +49 AA genotype (OR=2.0, CI=1.2-3.5, p=0.01) as well as their combined effect (OR=4.4, CI=2.0-9.7, p=0.0003). Our result supports the significant and combined effect of IL-1ra VNTR and CTLA-4 polymorphisms on MS justifying the need for further haplotype analysis in different populations. (c) 2006 Elsevier B.V. All rights reserved.",
journal = "Journal of Neuroimmunology",
title = "Association of polymorphisms in CTLA-4, IL-1ra and IL-1 beta genes with multiple sclerosis in Serbian population",
volume = "177",
number = "1-2",
pages = "146-150",
doi = "10.1016/j.jneuroim.2006.05.005"
}

Dinčić, E., Živković, M., Stanković, A., Obradović, D., Alavantić, D., Kostic, V.,& Raičević, R.. (2006). Association of polymorphisms in CTLA-4, IL-1ra and IL-1 beta genes with multiple sclerosis in Serbian population. in Journal of Neuroimmunology, 177(1-2), 146-150.
https://doi.org/10.1016/j.jneuroim.2006.05.005

Dinčić E, Živković M, Stanković A, Obradović D, Alavantić D, Kostic V, Raičević R. Association of polymorphisms in CTLA-4, IL-1ra and IL-1 beta genes with multiple sclerosis in Serbian population. in Journal of Neuroimmunology. 2006;177(1-2):146-150.
doi:10.1016/j.jneuroim.2006.05.005 .

Dinčić, Evica, Živković, Maja, Stanković, Aleksandra, Obradović, Dragana, Alavantić, Dragan, Kostic, Vladimir, Raičević, Ranko, "Association of polymorphisms in CTLA-4, IL-1ra and IL-1 beta genes with multiple sclerosis in Serbian population" in Journal of Neuroimmunology, 177, no. 1-2 (2006):146-150,
https://doi.org/10.1016/j.jneuroim.2006.05.005 . .