The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR
Нема приказа
Аутори
Živković, MajaStanković, Aleksandra
Dinčić, Evica
Popović, Milan
Popovic, Smiljana
Raičević, Ranko
Alavantić, Dragan
Чланак у часопису
Метаподаци
Приказ свих података о документуАпстракт
Background: Recently a high-resolution HLA and SNP map was defined and the analysis provided informative tag SNPs that capture much of the common variation in the MHC region. This concept enables detection of smaller number of SNPs, making it surrogate markers for haplotype associated with certain disease. The SNP rs3135388 was proposed as a tagging SNP for DRB1*1501/DQB1*0602 alleles, associated with MS. The aim of the study was to investigate the HLA rs3135388 genotypes in association with MS in patients from Serbia. Methods: Two hundred sixty nine consecutive patients from Serbia with relapse-remitting and secondary progressive MS were recruited for the study. Genomic DNA was isolated from peripheral blood cells. We designed the TaqMan assay for high-throughput genotyping of HLA rs3135388 on 7500 Real-Time PCR System. Results: We found significantly higher frequency of rs3135388 A allele carriers in MS patients compared to controls (p LT 0.001, chi(2)). In our population the carrier...s of one A allele had adjusted OR 2.09 (95% CI 1.41-3.09, p LT 0.001) for MS susceptibility. Conclusion: We assessed significant association of rs3135388 A allele carriership with MS in patients from Serbia. This HLA-DRB1*1501 surrogate marker is useful in association studies in MS. (C) 2009 Elsevier B.V. All rights reserved.
Кључне речи:
HLA-DRB1*1501 / rs3135388 / Gene polymorphism / Multiple sclerosisИзвор:
Clinica Chimica Acta, 2009, 406, 1-2, 27-30Финансирање / пројекти:
- Serbian Government [M145023]
DOI: 10.1016/j.cca.2009.05.004
ISSN: 0009-8981
PubMed: 19433080
WoS: 000268923900006
Scopus: 2-s2.0-67650327397
Колекције
Институција/група
VinčaTY - JOUR AU - Živković, Maja AU - Stanković, Aleksandra AU - Dinčić, Evica AU - Popović, Milan AU - Popovic, Smiljana AU - Raičević, Ranko AU - Alavantić, Dragan PY - 2009 UR - https://vinar.vin.bg.ac.rs/handle/123456789/3771 AB - Background: Recently a high-resolution HLA and SNP map was defined and the analysis provided informative tag SNPs that capture much of the common variation in the MHC region. This concept enables detection of smaller number of SNPs, making it surrogate markers for haplotype associated with certain disease. The SNP rs3135388 was proposed as a tagging SNP for DRB1*1501/DQB1*0602 alleles, associated with MS. The aim of the study was to investigate the HLA rs3135388 genotypes in association with MS in patients from Serbia. Methods: Two hundred sixty nine consecutive patients from Serbia with relapse-remitting and secondary progressive MS were recruited for the study. Genomic DNA was isolated from peripheral blood cells. We designed the TaqMan assay for high-throughput genotyping of HLA rs3135388 on 7500 Real-Time PCR System. Results: We found significantly higher frequency of rs3135388 A allele carriers in MS patients compared to controls (p LT 0.001, chi(2)). In our population the carriers of one A allele had adjusted OR 2.09 (95% CI 1.41-3.09, p LT 0.001) for MS susceptibility. Conclusion: We assessed significant association of rs3135388 A allele carriership with MS in patients from Serbia. This HLA-DRB1*1501 surrogate marker is useful in association studies in MS. (C) 2009 Elsevier B.V. All rights reserved. T2 - Clinica Chimica Acta T1 - The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR VL - 406 IS - 1-2 SP - 27 EP - 30 DO - 10.1016/j.cca.2009.05.004 ER -
@article{ author = "Živković, Maja and Stanković, Aleksandra and Dinčić, Evica and Popović, Milan and Popovic, Smiljana and Raičević, Ranko and Alavantić, Dragan", year = "2009", abstract = "Background: Recently a high-resolution HLA and SNP map was defined and the analysis provided informative tag SNPs that capture much of the common variation in the MHC region. This concept enables detection of smaller number of SNPs, making it surrogate markers for haplotype associated with certain disease. The SNP rs3135388 was proposed as a tagging SNP for DRB1*1501/DQB1*0602 alleles, associated with MS. The aim of the study was to investigate the HLA rs3135388 genotypes in association with MS in patients from Serbia. Methods: Two hundred sixty nine consecutive patients from Serbia with relapse-remitting and secondary progressive MS were recruited for the study. Genomic DNA was isolated from peripheral blood cells. We designed the TaqMan assay for high-throughput genotyping of HLA rs3135388 on 7500 Real-Time PCR System. Results: We found significantly higher frequency of rs3135388 A allele carriers in MS patients compared to controls (p LT 0.001, chi(2)). In our population the carriers of one A allele had adjusted OR 2.09 (95% CI 1.41-3.09, p LT 0.001) for MS susceptibility. Conclusion: We assessed significant association of rs3135388 A allele carriership with MS in patients from Serbia. This HLA-DRB1*1501 surrogate marker is useful in association studies in MS. (C) 2009 Elsevier B.V. All rights reserved.", journal = "Clinica Chimica Acta", title = "The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR", volume = "406", number = "1-2", pages = "27-30", doi = "10.1016/j.cca.2009.05.004" }
Živković, M., Stanković, A., Dinčić, E., Popović, M., Popovic, S., Raičević, R.,& Alavantić, D.. (2009). The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR. in Clinica Chimica Acta, 406(1-2), 27-30. https://doi.org/10.1016/j.cca.2009.05.004
Živković M, Stanković A, Dinčić E, Popović M, Popovic S, Raičević R, Alavantić D. The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR. in Clinica Chimica Acta. 2009;406(1-2):27-30. doi:10.1016/j.cca.2009.05.004 .
Živković, Maja, Stanković, Aleksandra, Dinčić, Evica, Popović, Milan, Popovic, Smiljana, Raičević, Ranko, Alavantić, Dragan, "The tag SNP for HLA-DRB1*1501, rs3135388, is significantly associated with multiple sclerosis susceptibility: Cost-effective high-throughput detection by real-time PCR" in Clinica Chimica Acta, 406, no. 1-2 (2009):27-30, https://doi.org/10.1016/j.cca.2009.05.004 . .