TY  - CONF
AU  - Čolović, Mirjana
AU  - Gajski, Goran
AU  - Ma, Tian
AU  - Isaković, Anđelka
AU  - Misirlić-Denčić, Sonja
AU  - Kortz, Urlich
AU  - Krstić, Danijela
PY  - 2021
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12932
AB  - Polyoxometalates (POMs) are discrete, negatively charged metal-oxo clusters of early transition metal ions in high oxidation states. Their biological significance has greatly increased in recent years because of their approved anticancer, antibiotic, and antidiabetic properties. However, toxicity studies have reported adverse effects after in vivo POM studies, which limits their potential application in biomedicine. The aim of this study is to evaluate the in vitro cyto- and genotoxic properties of two polyoxopalladates(II) containing tetravalent metal ion guests, SnPd12 and PbPd12, that were found to possess potent antitumor activities against the human acute promyelocytic cell line HL-60. For this purpose, blood samples obtained from a healthy female donor were treated with three different concentrations (12.5, 25, and 50 µmol/L) of the tested POPs, and incubated at 37 o C for 4 and 24 h, respectively. Cytotoxicity studies were performed on isolated human peripheral blood lymphocytes which were stained with acridine orange and ethidium bromide, and then viewed under a fluorescence microscope. The genotoxicity was tested in whole blood by alkaline comet assay (microgel electrophoresis). The percentage of tail DNA was used to determine the level of DNA damage. The obtained cytotoxicity results indicated that neither SnPd12 nor PbPd12 induced statistically significant alterations of cell viability related to the control, at all of the investigated concentrations. Moreover, the results of the comet assay showed that none of the tested POPs resulted in a statistically significant relative increase of tail DNA. Accordingly, both SnPd12 and PbPd12 could be considered as safe promising antileukaemic drugs from a cyto/genotoxicity point of view.
PB  - Zagreb, Croatia : Institute for Medical Research and Occupational Health
C3  - Archives of Industrial Hygiene and Toxicology
T1  - Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs
VL  - 72
IS  - Suppl. 1
SP  - 66
EP  - 66
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12932
ER  - 

@conference{
author = "Čolović, Mirjana and Gajski, Goran and Ma, Tian and Isaković, Anđelka and Misirlić-Denčić, Sonja and Kortz, Urlich and Krstić, Danijela",
year = "2021",
abstract = "Polyoxometalates (POMs) are discrete, negatively charged metal-oxo clusters of early transition metal ions in high oxidation states. Their biological significance has greatly increased in recent years because of their approved anticancer, antibiotic, and antidiabetic properties. However, toxicity studies have reported adverse effects after in vivo POM studies, which limits their potential application in biomedicine. The aim of this study is to evaluate the in vitro cyto- and genotoxic properties of two polyoxopalladates(II) containing tetravalent metal ion guests, SnPd12 and PbPd12, that were found to possess potent antitumor activities against the human acute promyelocytic cell line HL-60. For this purpose, blood samples obtained from a healthy female donor were treated with three different concentrations (12.5, 25, and 50 µmol/L) of the tested POPs, and incubated at 37 o C for 4 and 24 h, respectively. Cytotoxicity studies were performed on isolated human peripheral blood lymphocytes which were stained with acridine orange and ethidium bromide, and then viewed under a fluorescence microscope. The genotoxicity was tested in whole blood by alkaline comet assay (microgel electrophoresis). The percentage of tail DNA was used to determine the level of DNA damage. The obtained cytotoxicity results indicated that neither SnPd12 nor PbPd12 induced statistically significant alterations of cell viability related to the control, at all of the investigated concentrations. Moreover, the results of the comet assay showed that none of the tested POPs resulted in a statistically significant relative increase of tail DNA. Accordingly, both SnPd12 and PbPd12 could be considered as safe promising antileukaemic drugs from a cyto/genotoxicity point of view.",
publisher = "Zagreb, Croatia : Institute for Medical Research and Occupational Health",
journal = "Archives of Industrial Hygiene and Toxicology",
title = "Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs",
volume = "72",
number = "Suppl. 1",
pages = "66-66",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12932"
}

Čolović, M., Gajski, G., Ma, T., Isaković, A., Misirlić-Denčić, S., Kortz, U.,& Krstić, D.. (2021). Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs. in Archives of Industrial Hygiene and Toxicology
Zagreb, Croatia : Institute for Medical Research and Occupational Health., 72(Suppl. 1), 66-66.
https://hdl.handle.net/21.15107/rcub_vinar_12932

Čolović M, Gajski G, Ma T, Isaković A, Misirlić-Denčić S, Kortz U, Krstić D. Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs. in Archives of Industrial Hygiene and Toxicology. 2021;72(Suppl. 1):66-66.
https://hdl.handle.net/21.15107/rcub_vinar_12932 .

Čolović, Mirjana, Gajski, Goran, Ma, Tian, Isaković, Anđelka, Misirlić-Denčić, Sonja, Kortz, Urlich, Krstić, Danijela, "Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs" in Archives of Industrial Hygiene and Toxicology, 72, no. Suppl. 1 (2021):66-66,
https://hdl.handle.net/21.15107/rcub_vinar_12932 .