Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs
2021
Преузимање 🢃
Аутори
Čolović, MirjanaGajski, Goran
Ma, Tian
Isaković, Anđelka
Misirlić-Denčić, Sonja
Kortz, Urlich
Krstić, Danijela
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Polyoxometalates (POMs) are discrete, negatively charged metal-oxo clusters of early transition metal ions in high oxidation states. Their biological significance has greatly increased in recent years because of their approved anticancer, antibiotic, and antidiabetic properties. However, toxicity studies have reported adverse effects after in vivo POM studies, which limits their potential application in biomedicine. The aim of this study is to evaluate the in vitro cyto- and genotoxic properties of two polyoxopalladates(II) containing tetravalent metal ion guests, SnPd12 and PbPd12, that were found to possess potent antitumor activities against the human acute promyelocytic cell line HL-60. For this purpose, blood samples obtained from a healthy female donor were treated with three different concentrations (12.5, 25, and 50 µmol/L) of the tested POPs, and incubated at 37 o C for 4 and 24 h, respectively. Cytotoxicity studies were performed on isolated human peripheral blood lymphocytes... which were stained with acridine orange and ethidium bromide, and then viewed under a fluorescence microscope. The genotoxicity was tested in whole blood by alkaline comet assay (microgel electrophoresis). The percentage of tail DNA was used to determine the level of DNA damage. The obtained cytotoxicity results indicated that neither SnPd12 nor PbPd12 induced statistically significant alterations of cell viability related to the control, at all of the investigated concentrations. Moreover, the results of the comet assay showed that none of the tested POPs resulted in a statistically significant relative increase of tail DNA. Accordingly, both SnPd12 and PbPd12 could be considered as safe promising antileukaemic drugs from a cyto/genotoxicity point of view.
Кључне речи:
antitumour drugs / cell viability / comet assay / in vitro toxicity / polyoxopalladatesИзвор:
Archives of Industrial Hygiene and Toxicology, 2021, 72, Suppl. 1, 66-66Издавач:
- Zagreb, Croatia : Institute for Medical Research and Occupational Health
Напомена:
- 6th Croatian Congress of Toxicology with International Participation : CROTOX 2021 : Book of abstracts : October 3-6, 2021, Rabac, Croatia.
Колекције
Институција/група
VinčaTY - CONF AU - Čolović, Mirjana AU - Gajski, Goran AU - Ma, Tian AU - Isaković, Anđelka AU - Misirlić-Denčić, Sonja AU - Kortz, Urlich AU - Krstić, Danijela PY - 2021 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12932 AB - Polyoxometalates (POMs) are discrete, negatively charged metal-oxo clusters of early transition metal ions in high oxidation states. Their biological significance has greatly increased in recent years because of their approved anticancer, antibiotic, and antidiabetic properties. However, toxicity studies have reported adverse effects after in vivo POM studies, which limits their potential application in biomedicine. The aim of this study is to evaluate the in vitro cyto- and genotoxic properties of two polyoxopalladates(II) containing tetravalent metal ion guests, SnPd12 and PbPd12, that were found to possess potent antitumor activities against the human acute promyelocytic cell line HL-60. For this purpose, blood samples obtained from a healthy female donor were treated with three different concentrations (12.5, 25, and 50 µmol/L) of the tested POPs, and incubated at 37 o C for 4 and 24 h, respectively. Cytotoxicity studies were performed on isolated human peripheral blood lymphocytes which were stained with acridine orange and ethidium bromide, and then viewed under a fluorescence microscope. The genotoxicity was tested in whole blood by alkaline comet assay (microgel electrophoresis). The percentage of tail DNA was used to determine the level of DNA damage. The obtained cytotoxicity results indicated that neither SnPd12 nor PbPd12 induced statistically significant alterations of cell viability related to the control, at all of the investigated concentrations. Moreover, the results of the comet assay showed that none of the tested POPs resulted in a statistically significant relative increase of tail DNA. Accordingly, both SnPd12 and PbPd12 could be considered as safe promising antileukaemic drugs from a cyto/genotoxicity point of view. PB - Zagreb, Croatia : Institute for Medical Research and Occupational Health C3 - Archives of Industrial Hygiene and Toxicology T1 - Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs VL - 72 IS - Suppl. 1 SP - 66 EP - 66 UR - https://hdl.handle.net/21.15107/rcub_vinar_12932 ER -
@conference{ author = "Čolović, Mirjana and Gajski, Goran and Ma, Tian and Isaković, Anđelka and Misirlić-Denčić, Sonja and Kortz, Urlich and Krstić, Danijela", year = "2021", abstract = "Polyoxometalates (POMs) are discrete, negatively charged metal-oxo clusters of early transition metal ions in high oxidation states. Their biological significance has greatly increased in recent years because of their approved anticancer, antibiotic, and antidiabetic properties. However, toxicity studies have reported adverse effects after in vivo POM studies, which limits their potential application in biomedicine. The aim of this study is to evaluate the in vitro cyto- and genotoxic properties of two polyoxopalladates(II) containing tetravalent metal ion guests, SnPd12 and PbPd12, that were found to possess potent antitumor activities against the human acute promyelocytic cell line HL-60. For this purpose, blood samples obtained from a healthy female donor were treated with three different concentrations (12.5, 25, and 50 µmol/L) of the tested POPs, and incubated at 37 o C for 4 and 24 h, respectively. Cytotoxicity studies were performed on isolated human peripheral blood lymphocytes which were stained with acridine orange and ethidium bromide, and then viewed under a fluorescence microscope. The genotoxicity was tested in whole blood by alkaline comet assay (microgel electrophoresis). The percentage of tail DNA was used to determine the level of DNA damage. The obtained cytotoxicity results indicated that neither SnPd12 nor PbPd12 induced statistically significant alterations of cell viability related to the control, at all of the investigated concentrations. Moreover, the results of the comet assay showed that none of the tested POPs resulted in a statistically significant relative increase of tail DNA. Accordingly, both SnPd12 and PbPd12 could be considered as safe promising antileukaemic drugs from a cyto/genotoxicity point of view.", publisher = "Zagreb, Croatia : Institute for Medical Research and Occupational Health", journal = "Archives of Industrial Hygiene and Toxicology", title = "Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs", volume = "72", number = "Suppl. 1", pages = "66-66", url = "https://hdl.handle.net/21.15107/rcub_vinar_12932" }
Čolović, M., Gajski, G., Ma, T., Isaković, A., Misirlić-Denčić, S., Kortz, U.,& Krstić, D.. (2021). Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs. in Archives of Industrial Hygiene and Toxicology Zagreb, Croatia : Institute for Medical Research and Occupational Health., 72(Suppl. 1), 66-66. https://hdl.handle.net/21.15107/rcub_vinar_12932
Čolović M, Gajski G, Ma T, Isaković A, Misirlić-Denčić S, Kortz U, Krstić D. Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs. in Archives of Industrial Hygiene and Toxicology. 2021;72(Suppl. 1):66-66. https://hdl.handle.net/21.15107/rcub_vinar_12932 .
Čolović, Mirjana, Gajski, Goran, Ma, Tian, Isaković, Anđelka, Misirlić-Denčić, Sonja, Kortz, Urlich, Krstić, Danijela, "Cyto/genotoxicity evaluation of promising antileukaemic palladium-based drugs" in Archives of Industrial Hygiene and Toxicology, 72, no. Suppl. 1 (2021):66-66, https://hdl.handle.net/21.15107/rcub_vinar_12932 .