TY  - CONF
AU  - Vuković, Miodrag
AU  - Filipović, Lidija
AU  - Popović, Milica
AU  - Petrović, Nina
AU  - Tanić, Miljana
AU  - Janković, Radmila
AU  - Korać, Aleksandra
AU  - Čavić, Milena
PY  - 2023
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/12502
AB  - Introduction: The incidence of lung cancer (LC) in Serbia has increased over the last three decades. Up to 40% of patients with advanced non-small cell lung cancer (NSCLC) develop pleural effusion (PE). The aim of this study was to evaluate the usability of existing methods for isolation of extracellular vesicles from PE samples of patients with advanced non-small cell lung cancer and to characterize them for further use in the clinical/diagnostic/research setting. Material & Methods: PE samples diluted in PBS (1:1) from patients with advanced NSCLC were used. In-house spherical porous methacrylate-based copolymer coupled with VHH antibodies was used for the isolation of extracellular vesicles (EV) from PE samples. Flow-cytometry was performed for detection of exosomal markers. Results: A pool of PE was prepared from 5 patients with advanced NSCLC. Flow cytometry confirmed that the isolation of EVs was successful using the in-house affinity chromatography method. The presence of CD9 antigen was detected, as well as a decrease in the signal after the addition of Triton X-100. Further plans include the analysis of CD63 and CD81 antigens using flow cytometry, NTA analysis to determine the number and diameter of obtained vesicles, as well as TEM and SEM to determine their morphology. Discussion: We aim to investigate for the first time whether this method is applicable to pleural effusion samples as a new cancer liquid biopsy sample type. We also plan to evaluate the method in comparison with a commercial isolation kit.
PB  - Serbian Society for Extracellular Vesicles (SrbEVs)
PB  - Austrian Society for Extracellular Vesicles (ASEV)
PB  - Hungarian Society for Extracellular Vesicles (HSEV)
PB  - Slovenian Network for Extracellular Vesicles (SiN-EV)
C3  - Small New World 2.0 : Book of abstracts
T1  - Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer
SP  - 51
EP  - 51
UR  - https://hdl.handle.net/21.15107/rcub_vinar_12502
ER  - 

@conference{
author = "Vuković, Miodrag and Filipović, Lidija and Popović, Milica and Petrović, Nina and Tanić, Miljana and Janković, Radmila and Korać, Aleksandra and Čavić, Milena",
year = "2023",
abstract = "Introduction: The incidence of lung cancer (LC) in Serbia has increased over the last three decades. Up to 40% of patients with advanced non-small cell lung cancer (NSCLC) develop pleural effusion (PE). The aim of this study was to evaluate the usability of existing methods for isolation of extracellular vesicles from PE samples of patients with advanced non-small cell lung cancer and to characterize them for further use in the clinical/diagnostic/research setting. Material & Methods: PE samples diluted in PBS (1:1) from patients with advanced NSCLC were used. In-house spherical porous methacrylate-based copolymer coupled with VHH antibodies was used for the isolation of extracellular vesicles (EV) from PE samples. Flow-cytometry was performed for detection of exosomal markers. Results: A pool of PE was prepared from 5 patients with advanced NSCLC. Flow cytometry confirmed that the isolation of EVs was successful using the in-house affinity chromatography method. The presence of CD9 antigen was detected, as well as a decrease in the signal after the addition of Triton X-100. Further plans include the analysis of CD63 and CD81 antigens using flow cytometry, NTA analysis to determine the number and diameter of obtained vesicles, as well as TEM and SEM to determine their morphology. Discussion: We aim to investigate for the first time whether this method is applicable to pleural effusion samples as a new cancer liquid biopsy sample type. We also plan to evaluate the method in comparison with a commercial isolation kit.",
publisher = "Serbian Society for Extracellular Vesicles (SrbEVs), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), Slovenian Network for Extracellular Vesicles (SiN-EV)",
journal = "Small New World 2.0 : Book of abstracts",
title = "Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer",
pages = "51-51",
url = "https://hdl.handle.net/21.15107/rcub_vinar_12502"
}

Vuković, M., Filipović, L., Popović, M., Petrović, N., Tanić, M., Janković, R., Korać, A.,& Čavić, M.. (2023). Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer. in Small New World 2.0 : Book of abstracts
Serbian Society for Extracellular Vesicles (SrbEVs)., 51-51.
https://hdl.handle.net/21.15107/rcub_vinar_12502

Vuković M, Filipović L, Popović M, Petrović N, Tanić M, Janković R, Korać A, Čavić M. Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer. in Small New World 2.0 : Book of abstracts. 2023;:51-51.
https://hdl.handle.net/21.15107/rcub_vinar_12502 .

Vuković, Miodrag, Filipović, Lidija, Popović, Milica, Petrović, Nina, Tanić, Miljana, Janković, Radmila, Korać, Aleksandra, Čavić, Milena, "Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer" in Small New World 2.0 : Book of abstracts (2023):51-51,
https://hdl.handle.net/21.15107/rcub_vinar_12502 .