Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer
Само за регистроване кориснике
2023
Аутори
Vuković, MiodragFilipović, Lidija
Popović, Milica
Petrović, Nina
Tanić, Miljana
Janković, Radmila
Korać, Aleksandra
Čavić, Milena
Конференцијски прилог (Објављена верзија)
Метаподаци
Приказ свих података о документуАпстракт
Introduction: The incidence of lung cancer (LC) in Serbia has increased over the last three decades. Up to 40% of patients with advanced non-small cell lung cancer (NSCLC) develop pleural effusion (PE). The aim of this study was to evaluate the usability of existing methods for isolation of extracellular vesicles from PE samples of patients with advanced non-small cell lung cancer and to characterize them for further use in the clinical/diagnostic/research setting. Material & Methods: PE samples diluted in PBS (1:1) from patients with advanced NSCLC were used. In-house spherical porous methacrylate-based copolymer coupled with VHH antibodies was used for the isolation of extracellular vesicles (EV) from PE samples. Flow-cytometry was performed for detection of exosomal markers. Results: A pool of PE was prepared from 5 patients with advanced NSCLC. Flow cytometry confirmed that the isolation of EVs was successful using the in-house affinity chromatography method. The presence of CD9 an...tigen was detected, as well as a decrease in the signal after the addition of Triton X-100. Further plans include the analysis of CD63 and CD81 antigens using flow cytometry, NTA analysis to determine the number and diameter of obtained vesicles, as well as TEM and SEM to determine their morphology. Discussion: We aim to investigate for the first time whether this method is applicable to pleural effusion samples as a new cancer liquid biopsy sample type. We also plan to evaluate the method in comparison with a commercial isolation kit.
Извор:
Small New World 2.0 : Book of abstracts, 2023, 51-51Издавач:
- Serbian Society for Extracellular Vesicles (SrbEVs)
- Austrian Society for Extracellular Vesicles (ASEV)
- Hungarian Society for Extracellular Vesicles (HSEV)
- Slovenian Network for Extracellular Vesicles (SiN-EV)
Напомена:
- Small New World 2.0; September 4-5, Graz, Austria, 2023.
Колекције
Институција/група
VinčaTY - CONF AU - Vuković, Miodrag AU - Filipović, Lidija AU - Popović, Milica AU - Petrović, Nina AU - Tanić, Miljana AU - Janković, Radmila AU - Korać, Aleksandra AU - Čavić, Milena PY - 2023 UR - https://vinar.vin.bg.ac.rs/handle/123456789/12502 AB - Introduction: The incidence of lung cancer (LC) in Serbia has increased over the last three decades. Up to 40% of patients with advanced non-small cell lung cancer (NSCLC) develop pleural effusion (PE). The aim of this study was to evaluate the usability of existing methods for isolation of extracellular vesicles from PE samples of patients with advanced non-small cell lung cancer and to characterize them for further use in the clinical/diagnostic/research setting. Material & Methods: PE samples diluted in PBS (1:1) from patients with advanced NSCLC were used. In-house spherical porous methacrylate-based copolymer coupled with VHH antibodies was used for the isolation of extracellular vesicles (EV) from PE samples. Flow-cytometry was performed for detection of exosomal markers. Results: A pool of PE was prepared from 5 patients with advanced NSCLC. Flow cytometry confirmed that the isolation of EVs was successful using the in-house affinity chromatography method. The presence of CD9 antigen was detected, as well as a decrease in the signal after the addition of Triton X-100. Further plans include the analysis of CD63 and CD81 antigens using flow cytometry, NTA analysis to determine the number and diameter of obtained vesicles, as well as TEM and SEM to determine their morphology. Discussion: We aim to investigate for the first time whether this method is applicable to pleural effusion samples as a new cancer liquid biopsy sample type. We also plan to evaluate the method in comparison with a commercial isolation kit. PB - Serbian Society for Extracellular Vesicles (SrbEVs) PB - Austrian Society for Extracellular Vesicles (ASEV) PB - Hungarian Society for Extracellular Vesicles (HSEV) PB - Slovenian Network for Extracellular Vesicles (SiN-EV) C3 - Small New World 2.0 : Book of abstracts T1 - Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer SP - 51 EP - 51 UR - https://hdl.handle.net/21.15107/rcub_vinar_12502 ER -
@conference{ author = "Vuković, Miodrag and Filipović, Lidija and Popović, Milica and Petrović, Nina and Tanić, Miljana and Janković, Radmila and Korać, Aleksandra and Čavić, Milena", year = "2023", abstract = "Introduction: The incidence of lung cancer (LC) in Serbia has increased over the last three decades. Up to 40% of patients with advanced non-small cell lung cancer (NSCLC) develop pleural effusion (PE). The aim of this study was to evaluate the usability of existing methods for isolation of extracellular vesicles from PE samples of patients with advanced non-small cell lung cancer and to characterize them for further use in the clinical/diagnostic/research setting. Material & Methods: PE samples diluted in PBS (1:1) from patients with advanced NSCLC were used. In-house spherical porous methacrylate-based copolymer coupled with VHH antibodies was used for the isolation of extracellular vesicles (EV) from PE samples. Flow-cytometry was performed for detection of exosomal markers. Results: A pool of PE was prepared from 5 patients with advanced NSCLC. Flow cytometry confirmed that the isolation of EVs was successful using the in-house affinity chromatography method. The presence of CD9 antigen was detected, as well as a decrease in the signal after the addition of Triton X-100. Further plans include the analysis of CD63 and CD81 antigens using flow cytometry, NTA analysis to determine the number and diameter of obtained vesicles, as well as TEM and SEM to determine their morphology. Discussion: We aim to investigate for the first time whether this method is applicable to pleural effusion samples as a new cancer liquid biopsy sample type. We also plan to evaluate the method in comparison with a commercial isolation kit.", publisher = "Serbian Society for Extracellular Vesicles (SrbEVs), Austrian Society for Extracellular Vesicles (ASEV), Hungarian Society for Extracellular Vesicles (HSEV), Slovenian Network for Extracellular Vesicles (SiN-EV)", journal = "Small New World 2.0 : Book of abstracts", title = "Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer", pages = "51-51", url = "https://hdl.handle.net/21.15107/rcub_vinar_12502" }
Vuković, M., Filipović, L., Popović, M., Petrović, N., Tanić, M., Janković, R., Korać, A.,& Čavić, M.. (2023). Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer. in Small New World 2.0 : Book of abstracts Serbian Society for Extracellular Vesicles (SrbEVs)., 51-51. https://hdl.handle.net/21.15107/rcub_vinar_12502
Vuković M, Filipović L, Popović M, Petrović N, Tanić M, Janković R, Korać A, Čavić M. Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer. in Small New World 2.0 : Book of abstracts. 2023;:51-51. https://hdl.handle.net/21.15107/rcub_vinar_12502 .
Vuković, Miodrag, Filipović, Lidija, Popović, Milica, Petrović, Nina, Tanić, Miljana, Janković, Radmila, Korać, Aleksandra, Čavić, Milena, "Isolation and characterization of extracellular vesicles from pleural effusion samples of patients with advanced non-small cell lung cancer" in Small New World 2.0 : Book of abstracts (2023):51-51, https://hdl.handle.net/21.15107/rcub_vinar_12502 .