TY  - JOUR
AU  - Petrovic, S.
AU  - Takic, M.
AU  - Arsic, A.
AU  - Vučić, Vesna
AU  - Drakulić, Dunja R.
AU  - Milošević, Maja
AU  - Glibetić, Marija
PY  - 2014
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6095
AB  - The effects of 8-days treatment with 17 alpha-estradiol (33.3 mu g/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was found in BCO rats after the estradiol treatment. Both hormones elevated proportion of 18:1n-7 fatty acid in INT, but they failed to have such an effect in BCO. Estradiol increased 22:5n-3 and total n-3 polyunsaturated fatty acids (PUFA) in intact, and decreased 18:2n-6 in BCO rats. Significantly lower level of total n-3 was found in progesterone-treated than in estradiol-treated BCO rats. Given that n-3 PUFA have many beneficial effects on cell and tissue function, while n-6 PUFA have mostly the opposite effects, estradiol, rather than progesterone, was seen to improve plasma lipids and phospholipids FA profiles in INT and BCO animals. Estradiol significantly elevated the estimated activity of Delta 9-desaturases and progesterone of Delta 5-desaturase in BCO group, with no effects in INT rats.
T2  - Physiological Research
T1  - Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries
VL  - 63
IS  - 3
SP  - 331
EP  - 339
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6095
ER  - 

@article{
author = "Petrovic, S. and Takic, M. and Arsic, A. and Vučić, Vesna and Drakulić, Dunja R. and Milošević, Maja and Glibetić, Marija",
year = "2014",
abstract = "The effects of 8-days treatment with 17 alpha-estradiol (33.3 mu g/kg) and progesterone (1.7 mg/kg) on plasma lipids and fatty acid composition of plasma phospholipids were examined in intact (INT) and bilaterally common carotid arteries occluded (BCO) male Wistar rats. Significant decrease of triglyceride level was found in BCO rats after the estradiol treatment. Both hormones elevated proportion of 18:1n-7 fatty acid in INT, but they failed to have such an effect in BCO. Estradiol increased 22:5n-3 and total n-3 polyunsaturated fatty acids (PUFA) in intact, and decreased 18:2n-6 in BCO rats. Significantly lower level of total n-3 was found in progesterone-treated than in estradiol-treated BCO rats. Given that n-3 PUFA have many beneficial effects on cell and tissue function, while n-6 PUFA have mostly the opposite effects, estradiol, rather than progesterone, was seen to improve plasma lipids and phospholipids FA profiles in INT and BCO animals. Estradiol significantly elevated the estimated activity of Delta 9-desaturases and progesterone of Delta 5-desaturase in BCO group, with no effects in INT rats.",
journal = "Physiological Research",
title = "Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries",
volume = "63",
number = "3",
pages = "331-339",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6095"
}

Petrovic, S., Takic, M., Arsic, A., Vučić, V., Drakulić, D. R., Milošević, M.,& Glibetić, M.. (2014). Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries. in Physiological Research, 63(3), 331-339.
https://hdl.handle.net/21.15107/rcub_vinar_6095

Petrovic S, Takic M, Arsic A, Vučić V, Drakulić DR, Milošević M, Glibetić M. Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries. in Physiological Research. 2014;63(3):331-339.
https://hdl.handle.net/21.15107/rcub_vinar_6095 .

Petrovic, S., Takic, M., Arsic, A., Vučić, Vesna, Drakulić, Dunja R., Milošević, Maja, Glibetić, Marija, "Effect of Sex Hormones on Plasma Phospholipid Fatty Acid Composition in Intact Rats and Rats With Bilaterally Occluded Carotid Arteries" in Physiological Research, 63, no. 3 (2014):331-339,
https://hdl.handle.net/21.15107/rcub_vinar_6095 .

TY  - JOUR
AU  - Mladenovic, D.
AU  - Hrnčić, Dragan
AU  - Rašić-Marković, Aleksandra
AU  - Puškaš, Nela
AU  - Petrovic, S.
AU  - Stanojlović, Olivera
PY  - 2013
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/5226
AB  - Thioacetamide (TAA) is widely used as a model of hepatic encephalopathy (HE). The aim of our study was to investigate the effects of TAA on electroencephalographic (EEG) changes in rats and to compare them with human HE. Male Wistar rats were divided into groups: (1) saline-treated group and (2) TAA-treated groups: TAA(300) (300 mg/kg), TAA(600) (600 mg/kg), and TAA(900) (900 mg/kg). Daily dose of TAA (300 mg/kg) was administered intraperitoneally once (TAA(300)), twice (TAA(600)), or thrice (TAA(900)) in subsequent days. EEG changes were recorded about 24 h after the last dose of TAA. Absolute and relative power density in alpha bands were significantly higher in TAA(300) versus control group. In TAA(300), absolute beta power density was higher and relative beta power density was lower versus control group. Absolute alpha, theta, delta, and relative theta power were significantly lower, while relative power in delta band was significantly higher in TAA(900) versus control group (p LT 0.01). In conclusion, decrease in EEG voltage with an increase in delta relative power, which correspond to the EEG manifestations of severe HE in humans, was observed in TAA(900) group. Electrical activity in TAA(300) group correlates with mild HE in humans.
T2  - Human and Experimental Toxicology
T1  - Spectral analysis of thioacetamide-induced electroencephalographic changes in rats
VL  - 32
IS  - 1
SP  - 90
EP  - 100
DO  - 10.1177/0960327112456312
ER  - 

@article{
author = "Mladenovic, D. and Hrnčić, Dragan and Rašić-Marković, Aleksandra and Puškaš, Nela and Petrovic, S. and Stanojlović, Olivera",
year = "2013",
abstract = "Thioacetamide (TAA) is widely used as a model of hepatic encephalopathy (HE). The aim of our study was to investigate the effects of TAA on electroencephalographic (EEG) changes in rats and to compare them with human HE. Male Wistar rats were divided into groups: (1) saline-treated group and (2) TAA-treated groups: TAA(300) (300 mg/kg), TAA(600) (600 mg/kg), and TAA(900) (900 mg/kg). Daily dose of TAA (300 mg/kg) was administered intraperitoneally once (TAA(300)), twice (TAA(600)), or thrice (TAA(900)) in subsequent days. EEG changes were recorded about 24 h after the last dose of TAA. Absolute and relative power density in alpha bands were significantly higher in TAA(300) versus control group. In TAA(300), absolute beta power density was higher and relative beta power density was lower versus control group. Absolute alpha, theta, delta, and relative theta power were significantly lower, while relative power in delta band was significantly higher in TAA(900) versus control group (p LT 0.01). In conclusion, decrease in EEG voltage with an increase in delta relative power, which correspond to the EEG manifestations of severe HE in humans, was observed in TAA(900) group. Electrical activity in TAA(300) group correlates with mild HE in humans.",
journal = "Human and Experimental Toxicology",
title = "Spectral analysis of thioacetamide-induced electroencephalographic changes in rats",
volume = "32",
number = "1",
pages = "90-100",
doi = "10.1177/0960327112456312"
}

Mladenovic, D., Hrnčić, D., Rašić-Marković, A., Puškaš, N., Petrovic, S.,& Stanojlović, O.. (2013). Spectral analysis of thioacetamide-induced electroencephalographic changes in rats. in Human and Experimental Toxicology, 32(1), 90-100.
https://doi.org/10.1177/0960327112456312

Mladenovic D, Hrnčić D, Rašić-Marković A, Puškaš N, Petrovic S, Stanojlović O. Spectral analysis of thioacetamide-induced electroencephalographic changes in rats. in Human and Experimental Toxicology. 2013;32(1):90-100.
doi:10.1177/0960327112456312 .

Mladenovic, D., Hrnčić, Dragan, Rašić-Marković, Aleksandra, Puškaš, Nela, Petrovic, S., Stanojlović, Olivera, "Spectral analysis of thioacetamide-induced electroencephalographic changes in rats" in Human and Experimental Toxicology, 32, no. 1 (2013):90-100,
https://doi.org/10.1177/0960327112456312 . .

TY  - JOUR
AU  - Petrović, S.
AU  - Velickovic, N.
AU  - Stanojević, Ivana
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Stanojlović, Miloš R.
AU  - Horvat, Anica
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4512
AB  - Our results, as well as those of others, have indicated that 17 beta-estradiol (E2) exerts its nongenomic effects in neuronal cells by affecting plasma membrane Ca2+ flux. In neuronal cells mitochondria possess Ca2+ buffering properties as they both sequester and release Ca2+. The goal of this study was to examine the rapid non-genomic effect of E2 on mitochondria! Ca2+ transport in hippocampal synaptosomes from ovariectomised rats. In addition, we aimed to determine if, and to what extent, E2 receptors participated in mitochondria! Ca2+ transport modulation by E2 in vitro. E2-specific binding and Ca2+ transport was monitored. At physiological E2 concentrations (0.1-1.5 nmol/L), specific E2 binding to mitochondria isolated from hippocampal synaptosomes was detected with a B-max and K-m of 37.6 +/- 2.6 fmol/mg protein and 0.69 +/- 0.14 nmol/L of free E2, respectively. The main mitochondrial Ca2+ influx mechanism is the Ruthenium Red-sensitive uniporter driven by mitochondrial membrane potential. Despite no effect of E2 on Ca2+ influx, a physiological E2 concentration (0.5 nmol/L) protected mitochondrial membrane potential and consequently Ca2+ influx from the uncoupling agent carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (1 mu mol/L). In neuronal cells the predominant mitochondria! Ca2+ efflux mechanism is the Na+/Ca2+ exchanger. E2 caused Ca2+ efflux inhibition (by 46%) coupled with increased affinity of the Na+/Ca2+ exchanger for Na+. Using E2 receptor (ER alpha and ER beta) antagonists and agonists, we confirmed ER betas involvement in E2-induced mitochondrial membrane potential protection as well as Ca2+ efflux inhibition. In summary, our results indicate that the nongenomic neuromodulatory role of E2 in rat hippocampus is achieved by affecting mitochondria! Ca2+ transport via, in part, mitochondrial ER beta. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.
T2  - Neuroscience
T1  - Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus
VL  - 192
SP  - 195
EP  - 204
DO  - 10.1016/j.neuroscience.2011.06.030
ER  - 

@article{
author = "Petrović, S. and Velickovic, N. and Stanojević, Ivana and Milošević, Maja and Drakulić, Dunja R. and Stanojlović, Miloš R. and Horvat, Anica",
year = "2011",
abstract = "Our results, as well as those of others, have indicated that 17 beta-estradiol (E2) exerts its nongenomic effects in neuronal cells by affecting plasma membrane Ca2+ flux. In neuronal cells mitochondria possess Ca2+ buffering properties as they both sequester and release Ca2+. The goal of this study was to examine the rapid non-genomic effect of E2 on mitochondria! Ca2+ transport in hippocampal synaptosomes from ovariectomised rats. In addition, we aimed to determine if, and to what extent, E2 receptors participated in mitochondria! Ca2+ transport modulation by E2 in vitro. E2-specific binding and Ca2+ transport was monitored. At physiological E2 concentrations (0.1-1.5 nmol/L), specific E2 binding to mitochondria isolated from hippocampal synaptosomes was detected with a B-max and K-m of 37.6 +/- 2.6 fmol/mg protein and 0.69 +/- 0.14 nmol/L of free E2, respectively. The main mitochondrial Ca2+ influx mechanism is the Ruthenium Red-sensitive uniporter driven by mitochondrial membrane potential. Despite no effect of E2 on Ca2+ influx, a physiological E2 concentration (0.5 nmol/L) protected mitochondrial membrane potential and consequently Ca2+ influx from the uncoupling agent carbonyl cyanide 4-(trifluoromethoxy)phenylhydrazone (1 mu mol/L). In neuronal cells the predominant mitochondria! Ca2+ efflux mechanism is the Na+/Ca2+ exchanger. E2 caused Ca2+ efflux inhibition (by 46%) coupled with increased affinity of the Na+/Ca2+ exchanger for Na+. Using E2 receptor (ER alpha and ER beta) antagonists and agonists, we confirmed ER betas involvement in E2-induced mitochondrial membrane potential protection as well as Ca2+ efflux inhibition. In summary, our results indicate that the nongenomic neuromodulatory role of E2 in rat hippocampus is achieved by affecting mitochondria! Ca2+ transport via, in part, mitochondrial ER beta. (C) 2011 IBRO. Published by Elsevier Ltd. All rights reserved.",
journal = "Neuroscience",
title = "Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus",
volume = "192",
pages = "195-204",
doi = "10.1016/j.neuroscience.2011.06.030"
}

Petrović, S., Velickovic, N., Stanojević, I., Milošević, M., Drakulić, D. R., Stanojlović, M. R.,& Horvat, A.. (2011). Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus. in Neuroscience, 192, 195-204.
https://doi.org/10.1016/j.neuroscience.2011.06.030

Petrović S, Velickovic N, Stanojević I, Milošević M, Drakulić DR, Stanojlović MR, Horvat A. Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus. in Neuroscience. 2011;192:195-204.
doi:10.1016/j.neuroscience.2011.06.030 .

Petrović, S., Velickovic, N., Stanojević, Ivana, Milošević, Maja, Drakulić, Dunja R., Stanojlović, Miloš R., Horvat, Anica, "Inhibition of Mitochondrial Na+-Dependent Ca2+ Efflux By 17 Beta-Estradiol in the Rat Hippocampus" in Neuroscience, 192 (2011):195-204,
https://doi.org/10.1016/j.neuroscience.2011.06.030 . .

TY  - JOUR
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Petrovic, S.
AU  - Milošević, Maja
AU  - Jovanović, N.
AU  - Horvat, Anica
PY  - 2011
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/4603
AB  - A family of enzymes named ecto-nucleoside triphosphate diphosphohydrolase (NTPDases) catalyzes the termination of ATP and ADP actions. Three different NTPDases (NTPDase 1-3), differing in their preference for a substrate, have been localized in the brain of adult mammals. The goal of our study was to clarify ATP and ADP hydrolyzing activities and kinetic parameters of NTPDases in synaptic plasma membranes (SPM) isolated from 15-, 30-, 60- and 90-days-old female rat brains. ATP and ADP hydrolysis were maximal in the presence of Mg(2+) and showed insensitivity to ion-transporting ATPase inhibitors. The pronounced increase in both, ATP and ADP hydrolysis, were found in the SPM isolated from rats in the first month of life, stayed at the same level in the second month, and then decreased in adulthood. Kinetic analysis are also developmental-dependent, and together with the rate of ATP:ADP hydrolysis, point that all three NTPDases are present in SPM isolated from different developmental stages, with different, developmental-dependent proportion of activities. The lowest velocity and the highest affinity were observed for ATP hydrolyses, while the highest velocity and lowest affinity were detected for ADP hydrolyses in SPM isolated from 15-day old rats. Since specific ATP and ADP hydrolysis were lowest in this stage, we concluded that velocity is crucial for ATPase-, while affinity is for ADPase-part of NTPDases. Increased NTPDases activities, changes in their hydrolysis velocity and substrates affinities during rat postnatal development indicate involvement of adenine nucleotides in processes implicated to neuronal maturation and augmented neuroprotection.
T2  - Russian Journal of Physical Chemistry A
T1  - Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development
VL  - 85
IS  - 13
SP  - 2416
EP  - 2421
DO  - 10.1134/S0036024411130292
ER  - 

@article{
author = "Stanojević, Ivana and Drakulić, Dunja R. and Petrovic, S. and Milošević, Maja and Jovanović, N. and Horvat, Anica",
year = "2011",
abstract = "A family of enzymes named ecto-nucleoside triphosphate diphosphohydrolase (NTPDases) catalyzes the termination of ATP and ADP actions. Three different NTPDases (NTPDase 1-3), differing in their preference for a substrate, have been localized in the brain of adult mammals. The goal of our study was to clarify ATP and ADP hydrolyzing activities and kinetic parameters of NTPDases in synaptic plasma membranes (SPM) isolated from 15-, 30-, 60- and 90-days-old female rat brains. ATP and ADP hydrolysis were maximal in the presence of Mg(2+) and showed insensitivity to ion-transporting ATPase inhibitors. The pronounced increase in both, ATP and ADP hydrolysis, were found in the SPM isolated from rats in the first month of life, stayed at the same level in the second month, and then decreased in adulthood. Kinetic analysis are also developmental-dependent, and together with the rate of ATP:ADP hydrolysis, point that all three NTPDases are present in SPM isolated from different developmental stages, with different, developmental-dependent proportion of activities. The lowest velocity and the highest affinity were observed for ATP hydrolyses, while the highest velocity and lowest affinity were detected for ADP hydrolyses in SPM isolated from 15-day old rats. Since specific ATP and ADP hydrolysis were lowest in this stage, we concluded that velocity is crucial for ATPase-, while affinity is for ADPase-part of NTPDases. Increased NTPDases activities, changes in their hydrolysis velocity and substrates affinities during rat postnatal development indicate involvement of adenine nucleotides in processes implicated to neuronal maturation and augmented neuroprotection.",
journal = "Russian Journal of Physical Chemistry A",
title = "Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development",
volume = "85",
number = "13",
pages = "2416-2421",
doi = "10.1134/S0036024411130292"
}

Stanojević, I., Drakulić, D. R., Petrovic, S., Milošević, M., Jovanović, N.,& Horvat, A.. (2011). Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development. in Russian Journal of Physical Chemistry A, 85(13), 2416-2421.
https://doi.org/10.1134/S0036024411130292

Stanojević I, Drakulić DR, Petrovic S, Milošević M, Jovanović N, Horvat A. Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development. in Russian Journal of Physical Chemistry A. 2011;85(13):2416-2421.
doi:10.1134/S0036024411130292 .

Stanojević, Ivana, Drakulić, Dunja R., Petrovic, S., Milošević, Maja, Jovanović, N., Horvat, Anica, "Kinetic characterization of ecto-nucleoside triphosphate diphosphohydrolases in brain nerve terminals during rat postnatal development" in Russian Journal of Physical Chemistry A, 85, no. 13 (2011):2416-2421,
https://doi.org/10.1134/S0036024411130292 . .

TY  - CONF
AU  - Petrovic, S.
AU  - Milošević, Maja
AU  - Drakulić, Dunja R.
AU  - Stanojević, Ivana
AU  - Velickovic, N.
AU  - Horvat, Anica
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6803
C3  - FEBS Journal
T1  - 17beta-estradiol binding to synaptosomal mitochondria isolated from rat brain nucleus caudatus, hippocampus and brain steam
VL  - 276
SP  - 247
EP  - 247
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6803
ER  - 

@conference{
author = "Petrovic, S. and Milošević, Maja and Drakulić, Dunja R. and Stanojević, Ivana and Velickovic, N. and Horvat, Anica",
year = "2009",
journal = "FEBS Journal",
title = "17beta-estradiol binding to synaptosomal mitochondria isolated from rat brain nucleus caudatus, hippocampus and brain steam",
volume = "276",
pages = "247-247",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6803"
}

Petrovic, S., Milošević, M., Drakulić, D. R., Stanojević, I., Velickovic, N.,& Horvat, A.. (2009). 17beta-estradiol binding to synaptosomal mitochondria isolated from rat brain nucleus caudatus, hippocampus and brain steam. in FEBS Journal, 276, 247-247.
https://hdl.handle.net/21.15107/rcub_vinar_6803

Petrovic S, Milošević M, Drakulić DR, Stanojević I, Velickovic N, Horvat A. 17beta-estradiol binding to synaptosomal mitochondria isolated from rat brain nucleus caudatus, hippocampus and brain steam. in FEBS Journal. 2009;276:247-247.
https://hdl.handle.net/21.15107/rcub_vinar_6803 .

Petrovic, S., Milošević, Maja, Drakulić, Dunja R., Stanojević, Ivana, Velickovic, N., Horvat, Anica, "17beta-estradiol binding to synaptosomal mitochondria isolated from rat brain nucleus caudatus, hippocampus and brain steam" in FEBS Journal, 276 (2009):247-247,
https://hdl.handle.net/21.15107/rcub_vinar_6803 .

TY  - CONF
AU  - Drakulić, Dunja R.
AU  - Velickovic, N.
AU  - Milošević, Maja
AU  - Petrovic, S.
AU  - Stanojević, Ivana
AU  - Horvat, Anica
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6804
C3  - FEBS Journal
T1  - Dexamethasone effects on rat hippocampal apoptotic molecules expression
VL  - 276
SP  - 260
EP  - 260
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6804
ER  - 

@conference{
author = "Drakulić, Dunja R. and Velickovic, N. and Milošević, Maja and Petrovic, S. and Stanojević, Ivana and Horvat, Anica",
year = "2009",
journal = "FEBS Journal",
title = "Dexamethasone effects on rat hippocampal apoptotic molecules expression",
volume = "276",
pages = "260-260",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6804"
}

Drakulić, D. R., Velickovic, N., Milošević, M., Petrovic, S., Stanojević, I.,& Horvat, A.. (2009). Dexamethasone effects on rat hippocampal apoptotic molecules expression. in FEBS Journal, 276, 260-260.
https://hdl.handle.net/21.15107/rcub_vinar_6804

Drakulić DR, Velickovic N, Milošević M, Petrovic S, Stanojević I, Horvat A. Dexamethasone effects on rat hippocampal apoptotic molecules expression. in FEBS Journal. 2009;276:260-260.
https://hdl.handle.net/21.15107/rcub_vinar_6804 .

Drakulić, Dunja R., Velickovic, N., Milošević, Maja, Petrovic, S., Stanojević, Ivana, Horvat, Anica, "Dexamethasone effects on rat hippocampal apoptotic molecules expression" in FEBS Journal, 276 (2009):260-260,
https://hdl.handle.net/21.15107/rcub_vinar_6804 .

TY  - CONF
AU  - Velickovic, N.
AU  - Drakulić, Dunja R.
AU  - Petrovic, S.
AU  - Stanojević, Ivana
AU  - Milošević, Maja
AU  - Horvat, Anica
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6805
C3  - FEBS Journal
T1  - Radiation-induced HPA axis activation is associated with up-regulation of pro-inflammatory cytokines in rat brain
VL  - 276
SP  - 269
EP  - 269
UR  - https://hdl.handle.net/21.15107/rcub_vinar_6805
ER  - 

@conference{
author = "Velickovic, N. and Drakulić, Dunja R. and Petrovic, S. and Stanojević, Ivana and Milošević, Maja and Horvat, Anica",
year = "2009",
journal = "FEBS Journal",
title = "Radiation-induced HPA axis activation is associated with up-regulation of pro-inflammatory cytokines in rat brain",
volume = "276",
pages = "269-269",
url = "https://hdl.handle.net/21.15107/rcub_vinar_6805"
}

Velickovic, N., Drakulić, D. R., Petrovic, S., Stanojević, I., Milošević, M.,& Horvat, A.. (2009). Radiation-induced HPA axis activation is associated with up-regulation of pro-inflammatory cytokines in rat brain. in FEBS Journal, 276, 269-269.
https://hdl.handle.net/21.15107/rcub_vinar_6805

Velickovic N, Drakulić DR, Petrovic S, Stanojević I, Milošević M, Horvat A. Radiation-induced HPA axis activation is associated with up-regulation of pro-inflammatory cytokines in rat brain. in FEBS Journal. 2009;276:269-269.
https://hdl.handle.net/21.15107/rcub_vinar_6805 .

Velickovic, N., Drakulić, Dunja R., Petrovic, S., Stanojević, Ivana, Milošević, Maja, Horvat, Anica, "Radiation-induced HPA axis activation is associated with up-regulation of pro-inflammatory cytokines in rat brain" in FEBS Journal, 276 (2009):269-269,
https://hdl.handle.net/21.15107/rcub_vinar_6805 .

TY  - JOUR
AU  - Milošević, Maja
AU  - Petrovic, S.
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Velickovic, N.
AU  - Horvat, Anica
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6813
AB  - The aim of this study was to examine in vitro chelators ability to prevent copper-induced inhibition of rat myometrial ecto-ATPase activity. The effects of increasing CuSO(4) concentrations, in the absence and presence of 1 mmol/l EDTA, showed sigmoidal and complete inhibition relative to the control enzyme activity. IC(50) values, 1.15 x 10(-4) and 1.71 x 10(-3) mol/l in the absence and presence of EDTA, respectively, were determined by Hill analysis from experimental curves. According to the results presented in this work, 1 mmol/l EDTA increased by one order of magnitude CuSO(4) concentration for half-maximal inhibition (IC(50)), by decreasing Cu(2+) concentrations, available to form inactive CuATP(2-) complex.
T2  - Russian Journal of Physical Chemistry A
T1  - Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity
VL  - 83
IS  - 9
SP  - 1592
EP  - 1595
DO  - 10.1134/S0036024409090313
ER  - 

@article{
author = "Milošević, Maja and Petrovic, S. and Stanojević, Ivana and Drakulić, Dunja R. and Velickovic, N. and Horvat, Anica",
year = "2009",
abstract = "The aim of this study was to examine in vitro chelators ability to prevent copper-induced inhibition of rat myometrial ecto-ATPase activity. The effects of increasing CuSO(4) concentrations, in the absence and presence of 1 mmol/l EDTA, showed sigmoidal and complete inhibition relative to the control enzyme activity. IC(50) values, 1.15 x 10(-4) and 1.71 x 10(-3) mol/l in the absence and presence of EDTA, respectively, were determined by Hill analysis from experimental curves. According to the results presented in this work, 1 mmol/l EDTA increased by one order of magnitude CuSO(4) concentration for half-maximal inhibition (IC(50)), by decreasing Cu(2+) concentrations, available to form inactive CuATP(2-) complex.",
journal = "Russian Journal of Physical Chemistry A",
title = "Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity",
volume = "83",
number = "9",
pages = "1592-1595",
doi = "10.1134/S0036024409090313"
}

Milošević, M., Petrovic, S., Stanojević, I., Drakulić, D. R., Velickovic, N.,& Horvat, A.. (2009). Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity. in Russian Journal of Physical Chemistry A, 83(9), 1592-1595.
https://doi.org/10.1134/S0036024409090313

Milošević M, Petrovic S, Stanojević I, Drakulić DR, Velickovic N, Horvat A. Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity. in Russian Journal of Physical Chemistry A. 2009;83(9):1592-1595.
doi:10.1134/S0036024409090313 .

Milošević, Maja, Petrovic, S., Stanojević, Ivana, Drakulić, Dunja R., Velickovic, N., Horvat, Anica, "Effect of EDTA on copper-induced inhibition of rat myometrial ecto-ATPase activity" in Russian Journal of Physical Chemistry A, 83, no. 9 (2009):1592-1595,
https://doi.org/10.1134/S0036024409090313 . .

TY  - JOUR
AU  - Stanojević, Ivana
AU  - Drakulić, Dunja R.
AU  - Velickovic, N.
AU  - Milošević, Maja
AU  - Petrovic, S.
AU  - Horvat, Anica
PY  - 2009
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/6814
AB  - Cell membrane is highly sensitive to irradiation which, acting directly or indirectly, may disturb functions of constitutive proteins including membrane enzymes. Plasma membrane surface-located enzyme chain of ecto-nucleotide triphospho diphosphohydrolases (NTPDases) and 5-nucleotidase are involved in termination of cell purinergic signalization by hydrolyzing extracellular, excitatory adenosine triphosphate (ATP), as well as nucleotide di-, and mono-phosphate (ADP and AMP) to neuroprotective adenosine. Extracellular ATP, ADP, and AMP hydrolyzes were examined in purified synaptic plasma membranes after whole-body acute irradiation. All measurements were done 24 h after irradiation of developing (15-, 30-day-old) and adult (90-day-old) rats with low (50 cGy) and high (2 Gy) dose of gamma-rays. Both, high and low doses inhibited nucleotide hydrolyses in 15-day-old rats; in 30-day-old rats low dose of radiation inhibited ADP and AMP hydrolyses while high dose inhibited only ATP hydrolyse. In adult rats high dose induced no effects, while low dose stimulated nucleotides hydrolyses. According to obtained results it was concluded that ecto-nucleotidases of young rats are more sensitive to irradiation, since even low dose induces inhibition of ecto-nucleotidases activities. Ionizing radiation, by decreasing brain nucleotide hydrolyses in developing rats, induces accumulation of ATP and decreases production of adenosine in synaptic cleft which could be neurocytotoxic. On the contrary, in adult rats low dose of radiation stimulates NTPDase and 5-nucleotidase activity and protective adenosine production which indicates protective and adaptive mechanisms developed in adult brain neuronal cells.
T2  - Russian Journal of Physical Chemistry A
T1  - Effects of acute gamma-irradiation on extracellular adenine nucleotide hydrolysis in developing rat brain
VL  - 83
IS  - 9
SP  - 1596
EP  - 1601
DO  - 10.1134/S0036024409090325
ER  - 

@article{
author = "Stanojević, Ivana and Drakulić, Dunja R. and Velickovic, N. and Milošević, Maja and Petrovic, S. and Horvat, Anica",
year = "2009",
abstract = "Cell membrane is highly sensitive to irradiation which, acting directly or indirectly, may disturb functions of constitutive proteins including membrane enzymes. Plasma membrane surface-located enzyme chain of ecto-nucleotide triphospho diphosphohydrolases (NTPDases) and 5-nucleotidase are involved in termination of cell purinergic signalization by hydrolyzing extracellular, excitatory adenosine triphosphate (ATP), as well as nucleotide di-, and mono-phosphate (ADP and AMP) to neuroprotective adenosine. Extracellular ATP, ADP, and AMP hydrolyzes were examined in purified synaptic plasma membranes after whole-body acute irradiation. All measurements were done 24 h after irradiation of developing (15-, 30-day-old) and adult (90-day-old) rats with low (50 cGy) and high (2 Gy) dose of gamma-rays. Both, high and low doses inhibited nucleotide hydrolyses in 15-day-old rats; in 30-day-old rats low dose of radiation inhibited ADP and AMP hydrolyses while high dose inhibited only ATP hydrolyse. In adult rats high dose induced no effects, while low dose stimulated nucleotides hydrolyses. According to obtained results it was concluded that ecto-nucleotidases of young rats are more sensitive to irradiation, since even low dose induces inhibition of ecto-nucleotidases activities. Ionizing radiation, by decreasing brain nucleotide hydrolyses in developing rats, induces accumulation of ATP and decreases production of adenosine in synaptic cleft which could be neurocytotoxic. On the contrary, in adult rats low dose of radiation stimulates NTPDase and 5-nucleotidase activity and protective adenosine production which indicates protective and adaptive mechanisms developed in adult brain neuronal cells.",
journal = "Russian Journal of Physical Chemistry A",
title = "Effects of acute gamma-irradiation on extracellular adenine nucleotide hydrolysis in developing rat brain",
volume = "83",
number = "9",
pages = "1596-1601",
doi = "10.1134/S0036024409090325"
}

Stanojević, I., Drakulić, D. R., Velickovic, N., Milošević, M., Petrovic, S.,& Horvat, A.. (2009). Effects of acute gamma-irradiation on extracellular adenine nucleotide hydrolysis in developing rat brain. in Russian Journal of Physical Chemistry A, 83(9), 1596-1601.
https://doi.org/10.1134/S0036024409090325

Stanojević I, Drakulić DR, Velickovic N, Milošević M, Petrovic S, Horvat A. Effects of acute gamma-irradiation on extracellular adenine nucleotide hydrolysis in developing rat brain. in Russian Journal of Physical Chemistry A. 2009;83(9):1596-1601.
doi:10.1134/S0036024409090325 .

Stanojević, Ivana, Drakulić, Dunja R., Velickovic, N., Milošević, Maja, Petrovic, S., Horvat, Anica, "Effects of acute gamma-irradiation on extracellular adenine nucleotide hydrolysis in developing rat brain" in Russian Journal of Physical Chemistry A, 83, no. 9 (2009):1596-1601,
https://doi.org/10.1134/S0036024409090325 . .

TY  - JOUR
AU  - Horvat, Anica
AU  - Momić, Tatjana
AU  - Banjac, Ana
AU  - Petrović S.
AU  - Nikezić, Gordana S.
AU  - Demajo, Miroslav
PY  - 2006
UR  - https://vinar.vin.bg.ac.rs/handle/123456789/3040
AB  - The effect of drugs from the class of cardiac (methyldigoxin, verapamil, propranolol), antiepileptic ( carbamazepine), sedative (diazepam) and antihistaminic (promethazine) drugs on Na,K-ATPase activity of plasma membranes was studied in rat brain synaptosomes. Methyldigoxin in a concentration of 0.1 mmol/l inhibits enzyme activity by 80%. Verapamil, propranolol and promethazine in concentrations of 20, 20 and 2 mmol/l respectively, entirely inhibit the ATPase activity. Carbamazepine and diazepam in concentrations of 0.02-60 mmol/l have no effect on the activity of this enzyme. According to the drug concentrations that inhibit 50% of enzyme activity (IC50), the potency can be listed in the following order: methyldigoxin GT GT promethazine GT verapamil GT = propranolol. From the inhibition of commercially available purified Na, K-ATPase isolated from porcine cerebral cortex in the presence of chosen drugs, as well as from kinetic studies on synaptosomal plasma membranes, it may be concluded that the drugs inhibit enzyme activity, partly by acting directly on the enzyme proteins. Propranolol, verapamil and promethazine inhibitions acted in an uncompetitive manner. The results suggest that these three drugs may contribute to neurological dysfunctions and indicate the necessity to take into consideration the side effects of the investigated drugs during the treatment of various pathological conditions.
T2  - Physiological Research
T1  - Selective inhibition of brain Na,K-ATPase by drugs
VL  - 55
IS  - 3
SP  - 325
EP  - 338
UR  - https://hdl.handle.net/21.15107/rcub_vinar_3040
ER  - 

@article{
author = "Horvat, Anica and Momić, Tatjana and Banjac, Ana and Petrović S. and Nikezić, Gordana S. and Demajo, Miroslav",
year = "2006",
abstract = "The effect of drugs from the class of cardiac (methyldigoxin, verapamil, propranolol), antiepileptic ( carbamazepine), sedative (diazepam) and antihistaminic (promethazine) drugs on Na,K-ATPase activity of plasma membranes was studied in rat brain synaptosomes. Methyldigoxin in a concentration of 0.1 mmol/l inhibits enzyme activity by 80%. Verapamil, propranolol and promethazine in concentrations of 20, 20 and 2 mmol/l respectively, entirely inhibit the ATPase activity. Carbamazepine and diazepam in concentrations of 0.02-60 mmol/l have no effect on the activity of this enzyme. According to the drug concentrations that inhibit 50% of enzyme activity (IC50), the potency can be listed in the following order: methyldigoxin GT GT promethazine GT verapamil GT = propranolol. From the inhibition of commercially available purified Na, K-ATPase isolated from porcine cerebral cortex in the presence of chosen drugs, as well as from kinetic studies on synaptosomal plasma membranes, it may be concluded that the drugs inhibit enzyme activity, partly by acting directly on the enzyme proteins. Propranolol, verapamil and promethazine inhibitions acted in an uncompetitive manner. The results suggest that these three drugs may contribute to neurological dysfunctions and indicate the necessity to take into consideration the side effects of the investigated drugs during the treatment of various pathological conditions.",
journal = "Physiological Research",
title = "Selective inhibition of brain Na,K-ATPase by drugs",
volume = "55",
number = "3",
pages = "325-338",
url = "https://hdl.handle.net/21.15107/rcub_vinar_3040"
}

Horvat, A., Momić, T., Banjac, A., Petrović S., Nikezić, G. S.,& Demajo, M.. (2006). Selective inhibition of brain Na,K-ATPase by drugs. in Physiological Research, 55(3), 325-338.
https://hdl.handle.net/21.15107/rcub_vinar_3040

Horvat A, Momić T, Banjac A, Petrović S., Nikezić GS, Demajo M. Selective inhibition of brain Na,K-ATPase by drugs. in Physiological Research. 2006;55(3):325-338.
https://hdl.handle.net/21.15107/rcub_vinar_3040 .

Horvat, Anica, Momić, Tatjana, Banjac, Ana, Petrović S., Nikezić, Gordana S., Demajo, Miroslav, "Selective inhibition of brain Na,K-ATPase by drugs" in Physiological Research, 55, no. 3 (2006):325-338,
https://hdl.handle.net/21.15107/rcub_vinar_3040 .