In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy
2015
Authors
Dimić, IvanaCvijović-Alagić, Ivana
Obradović, Nataša
Petrović, Jelena
Putić, Slaviša S.
Rakin, Marko P.
Bugarski, Branko M.
Article (Published version)
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Show full item recordAbstract
Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity that represents an ideal environment for metallic ion release and biodegradation. The metallic ions released from dental materials can cause local and/or systemic adverse effects in the human body. Therefore, dental materials are required to possess appropriate mechanical, physical, chemical and biological properties. The biocompatibility of metallic materials is very important for dental applications. Accordingly, the aim of this study was to examine metallic ion release and cytotoxicity of Co-30Cr-5Mo cast alloy as the initial phase of biocompatibility evaluation. Determination of the viability of human (MRC-5) and animal (L929) fibroblast cells were conducted using three in vitro test methods: the colorimetric methyl-thiazol-tetrazolium (MTT) test, the dye exclusion test (DET) and the agar diffusion test (ADT). Furthermore, the morphology and growth of the cells were analyzed using s...canning electron microscopy (SEM). The obtained results indicated that Co-30Cr-5Mo alloy did not release harmful elements in concentrations high enough to have detrimental effects on human and animal fibroblasts under the given experimental conditions. Moreover, the fibroblast cells showed good adhesion on the surface of the Co-30Cr-5Mo alloy. Therefore, it could be concluded that Co-30Cr-5Mo alloy is a biocompatible material that could be safely used in dentistry.
Keywords:
Co-based alloy / biomaterials / cytotoxicity / fibroblastsSource:
Journal of the Serbian Chemical Society, 2015, 80, 12, 1541-1552Funding / projects:
- Novel encapsulation and enzyme technologies for designing of new biocatalysts and biologically active compounds targeting enhancement of food quality, safety and competitiveness (RS-MESTD-Integrated and Interdisciplinary Research (IIR or III)-46010)
- Micromechanical criteria of damage and fracture (RS-MESTD-Basic Research (BR or ON)-174004)
DOI: 10.2298/JSC150505070M
ISSN: 0352-5139
WoS: 000367186500008
Scopus: 2-s2.0-84983402048
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VinčaTY - JOUR AU - Dimić, Ivana AU - Cvijović-Alagić, Ivana AU - Obradović, Nataša AU - Petrović, Jelena AU - Putić, Slaviša S. AU - Rakin, Marko P. AU - Bugarski, Branko M. PY - 2015 UR - https://vinar.vin.bg.ac.rs/handle/123456789/870 AB - Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity that represents an ideal environment for metallic ion release and biodegradation. The metallic ions released from dental materials can cause local and/or systemic adverse effects in the human body. Therefore, dental materials are required to possess appropriate mechanical, physical, chemical and biological properties. The biocompatibility of metallic materials is very important for dental applications. Accordingly, the aim of this study was to examine metallic ion release and cytotoxicity of Co-30Cr-5Mo cast alloy as the initial phase of biocompatibility evaluation. Determination of the viability of human (MRC-5) and animal (L929) fibroblast cells were conducted using three in vitro test methods: the colorimetric methyl-thiazol-tetrazolium (MTT) test, the dye exclusion test (DET) and the agar diffusion test (ADT). Furthermore, the morphology and growth of the cells were analyzed using scanning electron microscopy (SEM). The obtained results indicated that Co-30Cr-5Mo alloy did not release harmful elements in concentrations high enough to have detrimental effects on human and animal fibroblasts under the given experimental conditions. Moreover, the fibroblast cells showed good adhesion on the surface of the Co-30Cr-5Mo alloy. Therefore, it could be concluded that Co-30Cr-5Mo alloy is a biocompatible material that could be safely used in dentistry. T2 - Journal of the Serbian Chemical Society T1 - In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy VL - 80 IS - 12 SP - 1541 EP - 1552 DO - 10.2298/JSC150505070M ER -
@article{ author = "Dimić, Ivana and Cvijović-Alagić, Ivana and Obradović, Nataša and Petrović, Jelena and Putić, Slaviša S. and Rakin, Marko P. and Bugarski, Branko M.", year = "2015", abstract = "Metallic materials, such as Co-Cr-Mo alloys, are exposed to aggressive conditions in the oral cavity that represents an ideal environment for metallic ion release and biodegradation. The metallic ions released from dental materials can cause local and/or systemic adverse effects in the human body. Therefore, dental materials are required to possess appropriate mechanical, physical, chemical and biological properties. The biocompatibility of metallic materials is very important for dental applications. Accordingly, the aim of this study was to examine metallic ion release and cytotoxicity of Co-30Cr-5Mo cast alloy as the initial phase of biocompatibility evaluation. Determination of the viability of human (MRC-5) and animal (L929) fibroblast cells were conducted using three in vitro test methods: the colorimetric methyl-thiazol-tetrazolium (MTT) test, the dye exclusion test (DET) and the agar diffusion test (ADT). Furthermore, the morphology and growth of the cells were analyzed using scanning electron microscopy (SEM). The obtained results indicated that Co-30Cr-5Mo alloy did not release harmful elements in concentrations high enough to have detrimental effects on human and animal fibroblasts under the given experimental conditions. Moreover, the fibroblast cells showed good adhesion on the surface of the Co-30Cr-5Mo alloy. Therefore, it could be concluded that Co-30Cr-5Mo alloy is a biocompatible material that could be safely used in dentistry.", journal = "Journal of the Serbian Chemical Society", title = "In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy", volume = "80", number = "12", pages = "1541-1552", doi = "10.2298/JSC150505070M" }
Dimić, I., Cvijović-Alagić, I., Obradović, N., Petrović, J., Putić, S. S., Rakin, M. P.,& Bugarski, B. M.. (2015). In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy. in Journal of the Serbian Chemical Society, 80(12), 1541-1552. https://doi.org/10.2298/JSC150505070M
Dimić I, Cvijović-Alagić I, Obradović N, Petrović J, Putić SS, Rakin MP, Bugarski BM. In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy. in Journal of the Serbian Chemical Society. 2015;80(12):1541-1552. doi:10.2298/JSC150505070M .
Dimić, Ivana , Cvijović-Alagić, Ivana, Obradović, Nataša, Petrović, Jelena, Putić, Slaviša S., Rakin, Marko P., Bugarski, Branko M., "In vitro biocompatibility assessment of Co-Cr-Mo dental cast alloy" in Journal of the Serbian Chemical Society, 80, no. 12 (2015):1541-1552, https://doi.org/10.2298/JSC150505070M . .