Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation
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2019
Authors
Joksić, GordanaValenta-Šobot, Ana
Filipović Tričković, Jelena G.
Maletić, Dejan
Puač, Nevena
Malović, Gordana N.
Petrović, Zoran Lj.
Lazović, Saša
Article (Published version)
,
© 2019, Indian Academy of Sciences
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In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4-1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2-3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h. © 2019, Indian Academy of Sciences.
Keywords:
Apoptosis / cold atmospheric plasma / ionizing radiation / primary human fibroblasts / repair kineticsSource:
Current Science, 2019, 116, 7, 1229-1233Funding / projects:
- Radiosensitivity of human genome (RS-MESTD-Basic Research (BR or ON)-173046)
DOI: 10.18520/cs/v116/i7/1229-1233
ISSN: 0011-3891
WoS: 000463900200034
Scopus: 2-s2.0-85064957173
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VinčaTY - JOUR AU - Joksić, Gordana AU - Valenta-Šobot, Ana AU - Filipović Tričković, Jelena G. AU - Maletić, Dejan AU - Puač, Nevena AU - Malović, Gordana N. AU - Petrović, Zoran Lj. AU - Lazović, Saša PY - 2019 UR - https://vinar.vin.bg.ac.rs/handle/123456789/8178 AB - In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4-1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2-3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h. © 2019, Indian Academy of Sciences. T2 - Current Science T1 - Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation VL - 116 IS - 7 SP - 1229 EP - 1233 DO - 10.18520/cs/v116/i7/1229-1233 ER -
@article{ author = "Joksić, Gordana and Valenta-Šobot, Ana and Filipović Tričković, Jelena G. and Maletić, Dejan and Puač, Nevena and Malović, Gordana N. and Petrović, Zoran Lj. and Lazović, Saša", year = "2019", abstract = "In this study we evaluate apoptosis time window of primary fibroblasts treated with cold atmospheric plasma (CAP), power range 0.4-1.4 W, for 30 sec, using γ-H2AX phosphorylation assay and flow cytometry. In contrast to irradiation where maximum of γ-H2AX foci appeared 30 min after irradiation and apoptosis 24 h later irrespective of radiation dose, treatment with CAP (power of 0.4 and 0.6) induces maximum of γ-H2AX foci 2 h after treatment. Apoptosis occurred in a power-dependent manner, with time shift of 2-3 h. Besides power-dependent time shift in apoptosis induction, apoptosis time window is the same and lasts for 2 h. © 2019, Indian Academy of Sciences.", journal = "Current Science", title = "Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation", volume = "116", number = "7", pages = "1229-1233", doi = "10.18520/cs/v116/i7/1229-1233" }
Joksić, G., Valenta-Šobot, A., Filipović Tričković, J. G., Maletić, D., Puač, N., Malović, G. N., Petrović, Z. Lj.,& Lazović, S.. (2019). Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation. in Current Science, 116(7), 1229-1233. https://doi.org/10.18520/cs/v116/i7/1229-1233
Joksić G, Valenta-Šobot A, Filipović Tričković JG, Maletić D, Puač N, Malović GN, Petrović ZL, Lazović S. Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation. in Current Science. 2019;116(7):1229-1233. doi:10.18520/cs/v116/i7/1229-1233 .
Joksić, Gordana, Valenta-Šobot, Ana, Filipović Tričković, Jelena G., Maletić, Dejan, Puač, Nevena, Malović, Gordana N., Petrović, Zoran Lj., Lazović, Saša, "Apoptosis time window induced by cold atmospheric plasma: Comparison with ionizing radiation" in Current Science, 116, no. 7 (2019):1229-1233, https://doi.org/10.18520/cs/v116/i7/1229-1233 . .