Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin
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Korićanac, LelaTodorović, Danijela V.
Popović, Nataša M.
Demajo, Miroslav
Ruždijić, Sabera
Ristić-Fira, Aleksandra
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Novel antineoplastic agents, 8-chloroadenosine 3,5-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, and apoptosis. The aim of this work was the in vitro study of either 8-Cl-cAMP or TR effects on B16/F10 and B16/C3 mouse melanoma cell growth and cell death. Significant cell growth inhibition was obtained after the application of 8-Cl-cAMP or TR. The presence and number of apoptotic cells was evaluated using agarose gel electrophoresis and flow cytometry. The number of apoptotic nuclei, after treatment with antineoplastic agents, did not significantly change in B16/F10 cells, although it did show a significant increase in B16/C3 cells. The expression of c-myc did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. The same results were obtained in... B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression showed a significant increase in B16/C3 cells after treatment with TR. Concerning the effects that the analyzed agents exhibited on melanoma cells and other cancer cells, further preclinical studies of these drugs will potentially lead to better understanding of the molecular mechanisms of their action and finally more efficient therapeutic approaches to malignant diseases.
Keywords:
melanoma / apoptosis / 8-Cl-cAMP / tiazofurinSource:
Annals of the New York Academy of Sciences, 2004, 1030, 384-392Note:
- Conference on Signal Transduction Pathways, Chromatin Structures and Gene Expression Mechanisms as Therapeutic Targets (Science Week 2004), Jan 25-31, 2004, Luxembourg, Luxembourg
DOI: 10.1196/annals.1329.048
ISSN: 0077-8923
PubMed: 15659821
WoS: 000228128100047
Scopus: 2-s2.0-15044352169
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VinčaTY - JOUR AU - Korićanac, Lela AU - Todorović, Danijela V. AU - Popović, Nataša M. AU - Demajo, Miroslav AU - Ruždijić, Sabera AU - Ristić-Fira, Aleksandra PY - 2004 UR - https://vinar.vin.bg.ac.rs/handle/123456789/6500 AB - Novel antineoplastic agents, 8-chloroadenosine 3,5-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, and apoptosis. The aim of this work was the in vitro study of either 8-Cl-cAMP or TR effects on B16/F10 and B16/C3 mouse melanoma cell growth and cell death. Significant cell growth inhibition was obtained after the application of 8-Cl-cAMP or TR. The presence and number of apoptotic cells was evaluated using agarose gel electrophoresis and flow cytometry. The number of apoptotic nuclei, after treatment with antineoplastic agents, did not significantly change in B16/F10 cells, although it did show a significant increase in B16/C3 cells. The expression of c-myc did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. The same results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression showed a significant increase in B16/C3 cells after treatment with TR. Concerning the effects that the analyzed agents exhibited on melanoma cells and other cancer cells, further preclinical studies of these drugs will potentially lead to better understanding of the molecular mechanisms of their action and finally more efficient therapeutic approaches to malignant diseases. T2 - Annals of the New York Academy of Sciences T1 - Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin VL - 1030 SP - 384 EP - 392 DO - 10.1196/annals.1329.048 ER -
@article{ author = "Korićanac, Lela and Todorović, Danijela V. and Popović, Nataša M. and Demajo, Miroslav and Ruždijić, Sabera and Ristić-Fira, Aleksandra", year = "2004", abstract = "Novel antineoplastic agents, 8-chloroadenosine 3,5-monophosphate (8-Cl-cAMP) and tiazofurin (TR), have been shown to be effective against different malignant cells. Through specific mechanisms of action they modulate the cellular signal transduction pathway, thereby causing growth inhibition, cell differentiation, and apoptosis. The aim of this work was the in vitro study of either 8-Cl-cAMP or TR effects on B16/F10 and B16/C3 mouse melanoma cell growth and cell death. Significant cell growth inhibition was obtained after the application of 8-Cl-cAMP or TR. The presence and number of apoptotic cells was evaluated using agarose gel electrophoresis and flow cytometry. The number of apoptotic nuclei, after treatment with antineoplastic agents, did not significantly change in B16/F10 cells, although it did show a significant increase in B16/C3 cells. The expression of c-myc did not significantly change in B16/F10 cells after treatment with 8-Cl-cAMP or TR. The same results were obtained in B16/C3 cells after treatment with 8-Cl-cAMP. The level of c-myc expression showed a significant increase in B16/C3 cells after treatment with TR. Concerning the effects that the analyzed agents exhibited on melanoma cells and other cancer cells, further preclinical studies of these drugs will potentially lead to better understanding of the molecular mechanisms of their action and finally more efficient therapeutic approaches to malignant diseases.", journal = "Annals of the New York Academy of Sciences", title = "Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin", volume = "1030", pages = "384-392", doi = "10.1196/annals.1329.048" }
Korićanac, L., Todorović, D. V., Popović, N. M., Demajo, M., Ruždijić, S.,& Ristić-Fira, A.. (2004). Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin. in Annals of the New York Academy of Sciences, 1030, 384-392. https://doi.org/10.1196/annals.1329.048
Korićanac L, Todorović DV, Popović NM, Demajo M, Ruždijić S, Ristić-Fira A. Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin. in Annals of the New York Academy of Sciences. 2004;1030:384-392. doi:10.1196/annals.1329.048 .
Korićanac, Lela, Todorović, Danijela V., Popović, Nataša M., Demajo, Miroslav, Ruždijić, Sabera, Ristić-Fira, Aleksandra, "Inhibition of B16 mouse melanoma cell growth and induction of apoptotic cell death with 8-chloroadenosine-3 5,-monophosphate and tiazofurin" in Annals of the New York Academy of Sciences, 1030 (2004):384-392, https://doi.org/10.1196/annals.1329.048 . .