Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity
2013
Authors
Cvijic, GordanaLakić, Iva
Vujovic, R.
Jasnic, N.
Djurasevic, S.
Dronjak, Slađana
Đorđević, Jelena D.
Article
Metadata
Show full item recordAbstract
The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chroni...c exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity.
Keywords:
Isolation / immobilization / IBAT / UCP-1 / MAO-A / SOD / catalaseSource:
Archives of Biological Sciences, 2013, 65, 3, 919-927Funding / projects:
- Effects of metabolic and nonmetabolic stressors on the expression and action of neuroendocrine regulators of energy homeostasis (RS-MESTD-Basic Research (BR or ON)-173023)
DOI: 10.2298/ABS1303919C
ISSN: 0354-4664
WoS: 000322348800014
Scopus: 2-s2.0-84882616747
Collections
Institution/Community
VinčaTY - JOUR AU - Cvijic, Gordana AU - Lakić, Iva AU - Vujovic, R. AU - Jasnic, N. AU - Djurasevic, S. AU - Dronjak, Slađana AU - Đorđević, Jelena D. PY - 2013 UR - https://vinar.vin.bg.ac.rs/handle/123456789/5603 AB - The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chronic exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity. T2 - Archives of Biological Sciences T1 - Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity VL - 65 IS - 3 SP - 919 EP - 927 DO - 10.2298/ABS1303919C ER -
@article{ author = "Cvijic, Gordana and Lakić, Iva and Vujovic, R. and Jasnic, N. and Djurasevic, S. and Dronjak, Slađana and Đorđević, Jelena D.", year = "2013", abstract = "The aim of this study was to examine whether the thermogenic potential of rat interscapular brown adipose tissue (IBAT) changes in response to acute and/or chronic exposure to non-thermal stressors (immobilization and isolation), by measuring the uncoupling protein 1 (UCP-1) content, MAO-A, SOD and CAT activities, as well as the number of IBAT sympathetic noradrenaline-containing nerve fibers. Both acute immobilization (2 h) and chronic isolation (21 days), as well as their combined effects, significantly increased the IBAT UCP-1 content in comparison to non-stressed animals. When applied individually, stressors increased the number of sympathetic fibers in comparison to controls, whereas in combination they decreased it. The activity of IBAT monoamine oxidase-A (MAO-A) decreased under the influence of each stressor independent of its type or duration. SOD activity coincided with MAO-A decrement, whereas CAT activity had an opposite pattern of changes. We conclude that acute and chronic exposure to non-thermal stressors, immobilization and isolation, respectively, affect the metabolic potential of rat IBAT, judging by the increase in UCP-1 content and sympathetic outflow. However, when acute immobilization was applied as a novel stressor to previously chronically isolated animals, an increase in the UCP-1 content was accompanied by a lower IBAT sympathetic outflow, suggesting that IBAT metabolic function under various stress condition is not solely dependent on SNS activity.", journal = "Archives of Biological Sciences", title = "Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity", volume = "65", number = "3", pages = "919-927", doi = "10.2298/ABS1303919C" }
Cvijic, G., Lakić, I., Vujovic, R., Jasnic, N., Djurasevic, S., Dronjak, S.,& Đorđević, J. D.. (2013). Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity. in Archives of Biological Sciences, 65(3), 919-927. https://doi.org/10.2298/ABS1303919C
Cvijic G, Lakić I, Vujovic R, Jasnic N, Djurasevic S, Dronjak S, Đorđević JD. Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity. in Archives of Biological Sciences. 2013;65(3):919-927. doi:10.2298/ABS1303919C .
Cvijic, Gordana, Lakić, Iva, Vujovic, R., Jasnic, N., Djurasevic, S., Dronjak, Slađana, Đorđević, Jelena D., "Single and Combined Effects of Acute and Chronic Non-Thermal Stressors on Rat Interscapular Brown Adipose Tissue Metabolic Activity" in Archives of Biological Sciences, 65, no. 3 (2013):919-927, https://doi.org/10.2298/ABS1303919C . .